A gene‐centered C. elegans protein–DNA interaction network provides a framework for functional predictions by Juan I Fuxman Bass, Carles Pons, Lucie Kozlowski, John S Reece‐Hoyes, Shaleen Shrestha, Amy D Holdorf, Akihiro Mori, Chad L Myers, Albertha JM Walhout

“…We used this network as a backbone to predict TF binding sites for 77 TFs, two‐thirds of which are novel, as well as integrate gene expression, protein–protein interaction, and phenotypic data to predict regulatory and biological functions for multiple genes and TFs.…”
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Quantification and discovery of sequence determinants of protein‐per‐mRNA amount in 29 human tissues by Basak Eraslan, Dongxue Wang, Mirjana Gusic, Holger Prokisch, Björn M Hallström, Mathias Uhlén, Anna Asplund, Frederik Pontén, Thomas Wieland, Thomas Hopf, Hannes Hahne, Bernhard Kuster, Julien Gagneur

“…While PTR ratios span more than 2 orders of magnitude, our integrative model predicts PTR ratios at a median precision of 3.2‐fold. …”
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ECM1 expression in chronic liver disease: Regulation by EGF/STAT1 and IFNγ/NRF2 signalling by Yujia Li, Chenjun Huang, Weiguo Fan, Seddik Hammad, Cyrill Géraud, Lea Berger, Shanshan Wang, Ye Yao, Chenhao Tong, Claudia Rubie, Laura Kim Feiner, Zeribe C. Nwosu, Frederik Link, Pia Erdösi, Weronika Piorońska, Kerry Gould, Christoph Meyer, Rilu Feng, Hui Liu, Chen Shao, Bing Sun, Huiguo Ding, Roman Liebe, Matthias P.A. Ebert, Honglei Weng, Matthias Glanemann, Chunfang Gao, Peter ten Dijke, Steven Dooley, Sai Wang

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Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration by Tongbin Liu, Tongbin Liu, Akram Hassan, Matheel Zohair Yousif Alrawas, Caiyun Cui, Zaihan Ariffin

“…The pH-dependent release system maintained effective Cu2+ levels while reducing cytotoxicity.DiscussionBy integrating PDA-mediated Cu2+ coordination with PCL nanofibers, we created a multifunctional platform that balances antimicrobial defense and bone regeneration. …”
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Pan‐Variant SARS‐CoV‐2 Vaccines Induce Protective Immunity by Targeting Conserved Epitopes by Masaud Shah, Sung Ung Moon, Ji‐Yon Shin, Ji‐Hye Choi, Doyoon Kim, Hyun Goo Woo

“…Unique peptides with conserved MHC‐anchoring residues are identified, enabling binding to a spectrum of MHC‐I and MHC‐II haplotypes. …”
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NERVE 2.0: boosting the new enhanced reverse vaccinology environment via artificial intelligence and a user-friendly web interface by Andrea Conte, Nicola Gulmini, Francesco Costa, Matteo Cartura, Felix Bröhl, Francesco Patanè, Francesco Filippini

“…When NERVE (New Enhanced RV Environment), the first RV software integrating tools to perform the selection of VCs, was released, it prompted further development in the field. …”
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Dual-labeled anti-GD2 targeted probe for intraoperative molecular imaging of neuroblastoma by Lauren Taylor Rosenblum, ReidAnn E. Sever, Ryan Gilbert, David Guerrero, Sarah R. Vincze, Dominic M. Menendez, Peggy A. Birikorang, Mikayla R. Rodgers, Ambika Parmar Jaswal, Alexander C. Vanover, Joseph D. Latoche, Angel G. Cortez, Kathryn E. Day, Lesley M. Foley, Chaim T. Sneiderman, Itay Raphael, T. Kevin Hitchens, Jessie R. Nedrow, Gary Kohanbash, W. Barry Edwards, Marcus M. Malek

“…Abstract Background Surgical resection is integral for the treatment of neuroblastoma, the most common extracranial solid malignancy in children. …”
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A Novel Parvovirus Associated with the Whitefly <i>Bemisia tabaci</i> by Fani Gousi, Zineb Belabess, Nathalie Laboureau, Michel Peterschmitt, Mikhail M. Pooggin

“…Furthermore, we provide evidence for the genetic variants of BtaDV circulating in whitefly populations and for its partial sequences integrated into the <i>B. tabaci</i> genome, with one integrant locus potentially expressing a fusion protein composed of viral Rep endonuclease and host DNA-binding domains. …”
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Exploring the molecular mechanisms of subarachnoid hemorrhage and potential therapeutic targets: insights from bioinformatics and drug prediction by Yi Liu, Yang Zhang, Huan Wei, Li Wang, Lishang Liao

“…Its treatment remains a significant challenge, especially due to the incomplete understanding of its molecular mechanisms. In this study, we integrated comprehensive bioinformatics analyses with experimental validation to explore the potential pathogenic mechanisms and immune cell infiltration characteristics of SAH, aiming to identify novel diagnostic biomarkers and therapeutic targets. …”
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Proton magnetic resonance spectroscopy reveals neuroprotection by oral minocycline in a nonhuman primate model of accelerated NeuroAIDS. by Eva-Maria Ratai, Jeffrey P Bombardier, Chan-Gyu Joo, Lakshmanan Annamalai, Tricia H Burdo, Jennifer Campbell, Robert Fell, Reza Hakimelahi, Julian He, Patrick Autissier, Margaret R Lentz, Elkan F Halpern, Eliezer Masliah, Kenneth C Williams, Susan V Westmoreland, R Gilberto González

“…Astrogliosis and microglial activation were quantified by measuring glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (IBA-1), respectively. …”
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Therapeutic hemoglobin levels after gene transfer in β-thalassemia mice and in hematopoietic cells of β-thalassemia and sickle cells disease patients. by Laura Breda, Carla Casu, Sara Gardenghi, Nicoletta Bianchi, Luca Cartegni, Mohandas Narla, Karina Yazdanbakhsh, Marco Musso, Deepa Manwani, Jane Little, Lawrence B Gardner, Dorothy A Kleinert, Eugenia Prus, Eitan Fibach, Robert W Grady, Patricia J Giardina, Roberto Gambari, Stefano Rivella

“…We found that insertion of an ankyrin insulator leads to higher, potentially therapeutic levels of human β-globin through a novel mechanism that links the rate of transcription of the transgenic β-globin mRNA during erythroid differentiation with polysomal binding and efficient translation, as reported here for the first time. …”
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Polyethyleneimine-functionalized iron oxide nanoparticle-based method for isolation of Deoxyribonucleic Acid (DNA) from saliva samples in forensic investigations by Imran Khan, Gaurav Kaushik, Chaitenya Verma, Vinay Kumar, Richa Vashishtha

“…DNA yield, purity, and integrity were assessed using UV–vis spectrophotometry and agarose gel electrophoresis. …”
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FUNDING MECHANISMS FOR IMPLEMENTATION OF MARINE STRATEGY FRAMEWORK DIRECTIVE: EU EXPERIENCE by S. Kniaz, E. Pavlenko, O. Rubel, O. Zavnerchik

“…In particular, one of the objectives of the strategy is the development of non-legally binding and practical documents, such as co-financing of MSFD measures. …”
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Innovation in Comprehensive Transportation Network Planning in the Context of National Spatial Development: Institutional Constraints and Policy Responses by Huanyu Yang, Wei Huang, Dong Yang, Ying Jiang

“…This study investigates the institutional innovation pathways for integrating comprehensive transportation networks into China’s territorial spatial planning system, with a focus on resolving the conflicts between ecological conservation and infrastructure development. …”
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Intervention effect and mechanism of Dracocephalum moldavica L. extract on bleomycin-induced pulmonary fibrosis in rats by Xiaoyu SUN, Li CHEN, Ruifang GAO, Hui YU, Min JIN

“…The molecular docking results showed that the binding energy between the active components (quercetin, apigenin, aesculetin, quercitrin) of Dracocephalum moldavica L. extract and the core targets of pulmonary fibrosis (TNF, IL-6, ALB, AKT1) were all <-29.288 kJ·mol−1, indicating good binding ability. …”
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Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugatesResearch in context by Yuichi Adachi, Kotaro Miyake, Kika Ohira, Shingo Satoh, Kentaro Masuhiro, Ryuya Edahiro, Yuya Shirai, Maiko Naito, Yujiro Naito, Takayuki Shiroyama, Shohei Koyama, Haruhiko Hirata, Kota Iwahori, Izumi Nagatomo, Yoshito Takeda, Atsushi Kumanogoh

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Tetra-anionic porphyrin mimics protein-protein interactions between regulatory particles and the catalytic core, allosterically activating human 20S proteasome by A. M. Santoro, M. Persico, A. D’Urso, A. Cunsolo, O. Tkachuk, D. Milardi, R. Purrello, G. R. Tundo, D. Sbardella, P. A. Osmulski, M. Gaczynska, M. Coletta, C. Fattorusso

“…Based on the hypothesis of an electrostatic key code driving catalytic core particle (20S) activation by regulatory particles (RPs), we identified the tetra-anionic meso-Tetrakis(4-sulphonatophenyl)-porphyrin (H2TPPS) as a new activator of human proteasome. By means of an integrated approach, including bioinformatics, enzymatic kinetic analysis, atomic force microscopy, and dynamic docking simulations, we show how binding of H2TPPS affects the closed/open conformational equilibrium of human 20S to ultimately promote substrate gate opening and proteolytic activity. …”
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Galectin-1 in Cardiovascular Pathogenesis: Unraveling Dual Roles and Mechanistic Insights in Emerging Research by Po-Yuan Chen, Chun-Yao Cheng, Chun-Chao Chen, Huan-Yuan Chen, Ju-Chi Liu, Wen-Rui Hao, Tzu-Hurng Cheng, Jin-Jer Chen

“…Galectin-1 (Gal-1), a β-galactoside-binding lectin, plays a complex role in cardiovascular diseases (CVDs), exerting both protective and pathological effects depending on the context. …”
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Microbial degradation and watershed weathering jointly regulate soil organic matter stabilization in alpine wetlands by Bin Niu, Tianzhu Lei, Qiuyu Chen, Yilun Hu, Hailiang Dong, Yibo Yang, Jizhong Zhou, Gengxin Zhang

“…Our results highlight the need to integrate weathering and microbial functions into soil carbon models in alpine wetlands responding to climate warming.…”
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Design of orthogonal genetic switches based on a crosstalk map of σs, anti‐σs, and promoters by Virgil A Rhodius, Thomas H Segall‐Shapiro, Brian D Sharon, Amar Ghodasara, Ekaterina Orlova, Hannah Tabakh, David H Burkhardt, Kevin Clancy, Todd C Peterson, Carol A Gross, Christopher A Voigt

“…Abstract Cells react to their environment through gene regulatory networks. Network integrity requires minimization of undesired crosstalk between their biomolecules. …”
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