LncRNA SLNCR phenocopies the E2F1 DNA binding site to promote melanoma progression by Kushani Shah, Eleni Anastasakou, Leinal Sejour, Yufei Wang, Leon Wert-Lamas, Christopher Rauchet, Sabine Studer, Simon Goller, Robert J. Distel, Wayne Marasco, Lalith Perera, Ioannis S. Vlachos, Carl D. Novina

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The Prognostic Potential of Insulin-like Growth Factor-Binding Protein 1 for Cardiovascular Complications in Peripheral Artery Disease by Ben Li, Farah Shaikh, Houssam Younes, Batool Abuhalimeh, Abdelrahman Zamzam, Rawand Abdin, Mohammad Qadura

“…Of all proteins studied, only insulin-like growth factor-binding protein 1 (IGFBP-1) showed a significant elevation among patients who suffered MACE versus those who remained event-free (20.66 [SD 3.91] vs. 13.94 [SD 3.80] pg/mL; <i>p</i> = 0.012). …”
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Comparative molecular dynamics simulation of apo and holo forms of the P53 mutant C176F: a structural perspective by R. Hephzibah Cathryn, C. George Priya Doss

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HpFBH3 transactivates HpCO7 via binding to the E-boxes in the promoter and may accelerate flower formation in pitaya by Xiaowei Cai, Ling Xiao, Xiangmei Nie, Qiandong Hou, Sulin Wen, Kun Yang, Xiaopeng Wen

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Key RNA-binding proteins in renal fibrosis: a comprehensive bioinformatics and machine learning framework for diagnostic and therapeutic insights by Jie Chen, Binghan Zhang, Qixuan Huang, Ronghua Fang, Ziyu Ren, Dongfang Liu

“…Emerging evidence suggests RNA-binding proteins (RBPs) are pivotal in regulating cellular mechanisms underlying fibrosis.Methods Utilizing an extensive GEO dataset (175 renal fibrosis and 99 normal kidney samples), we identified and validated key RBPs through integrated bioinformatics and machine learning approaches, including lasso and logistic regression models. …”
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Widespread 3′UTR capped RNAs derive from G-rich regions in proximity to AGO2 binding sites by Nejc Haberman, Holly Digby, Rupert Faraway, Rebecca Cheung, Anob M. Chakrabarti, Andrew M. Jobbins, Callum Parr, Kayoko Yasuzawa, Takeya Kasukawa, Chi Wai Yip, Masaki Kato, Hazuki Takahashi, Piero Carninci, Santiago Vernia, Jernej Ule, Christopher R. Sibley, Aida Martinez-Sanchez, Boris Lenhard

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A single mutation in Taiwanese H6N1 influenza hemagglutinin switches binding to human‐type receptors by Robert P de Vries, Netanel Tzarum, Wenjie Peng, Andrew J Thompson, Iresha N Ambepitiya Wickramasinghe, Alba T Torrents de la Pena, Marielle J van Breemen, Kim M Bouwman, Xueyong Zhu, Ryan McBride, Wenli Yu, Rogier W Sanders, Monique H Verheije, Ian A Wilson, James C Paulson

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Vaccinia Virus Immunomodulator A46: A Lipid and Protein-Binding Scaffold for Sequestering Host TIR-Domain Proteins. by Sofiya Fedosyuk, Gustavo Arruda Bezerra, Katharina Radakovics, Terry K Smith, Massimo Sammito, Nina Bobik, Adam Round, Lynn F Ten Eyck, Kristina Djinović-Carugo, Isabel Usón, Tim Skern

“…Using a combination of high resolution X-ray structures of the N- and C-terminal domains and SAXS analysis of full-length protein A46(1-240), we present here a structural model of A46 in a tetrameric assembly. Integrating affinity measurements and structural data, we propose how A46 simultaneously interferes with several TIR-domain containing proteins to inhibit NF-κB activation and postulate that A46 employs a bipartite binding arrangement to sequester the host immune adaptors TRAM and MyD88.…”
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Genome-wide identification, transcriptional profiling, and miRNA-binding site analysis of the LBD gene family in the camphor tree by Jiaqi Zhang, Luyang Shan, Haoran Qi, Haoran Qi, Caihui Chen, Yongda Zhong, Meng Xu

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From immune evasion to broad in silico binding: computational optimization of SARS-CoV-2 RBD-targeting nanobody by Shuyuan Cao, Bo Sun, Feng Gao, Feng Gao, Feng Gao

“…Building on these findings, we established a streamlined nanobody optimization pipeline integrating high-throughput mutagenesis of complementarity-determining regions (CDRs) and hotspot residues, protein-protein docking, and MD simulations.ResultsMD analysis confirmed that the immune evasion mechanism of KP.3/XEC is significantly associated with the Q493E mutation, which weakens electrostatic interactions between the nanobodies and the receptor binding domain (RBD). …”
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Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src by Zhenxi Li, Xinghai Yang, Ruifeng Fu, Zhipeng Wu, Shengzhao Xu, Jian Jiao, Ming Qian, Long Zhang, Chunbiao Wu, Tianying Xie, Jiqiang Yao, Zhixiang Wu, Wenjun Li, Guoli Ma, Yu You, Yihua Chen, Han-kun Zhang, Yiyun Cheng, Xiaolong Tang, Pengfei Wu, Gewei Lian, Haifeng Wei, Jian Zhao, Jianrong Xu, Lianzhong Ai, Stefan Siwko, Yue Wang, Jin Ding, Gaojie Song, Jian Luo, Mingyao Liu, Jianru Xiao

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Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells by Ana-Maria Nitulescu, Weijie Du, Viktor Glaser, Jonas Kath, Eric J. Aird, Grégoire Cullot, Robert Greensmith, Nanna Steengaard Mikkelsen, Maik Stein, Rasmus O. Bak, Michael Kaminski, Jacob E. Corn, Dimitrios L. Wagner

“…Here, we investigate CTS design for AsCas12a Ultra by exploring PAM orientation and binding requirements. Additionally, we rule out ssDNase activity of AsCas12a under cell-physiological Mg2+ conditions. …”
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Cartilage-bone inspired the construction of soft-hard composite material with excellent interfacial binding performance and low friction for artificial joints by Qin Chen, Xinyue Zhang, Siyu Liu, Kai Chen, Cunao Feng, Xiaowei Li, Jianwei Qi, Yong Luo, Hongtao Liu, Dekun Zhang

“…The load-bearing properties, binding performance, and tribological behaviors for different forms of the soft-hard integrated materials were investigated. …”
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Protein phosphatase-1 regulates the binding of filamin C to FILIP1 in cultured skeletal muscle cells under mechanical stress by Thomas Kokot, Johannes P. Zimmermann, Anja N. Schwäble, Lena Reimann, Anna L. Herr, Nico Höfflin, Maja Köhn, Bettina Warscheid

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Functional Involvement of TANK-Binding Kinase 1 in the MyD88-Dependent NF-κB Pathway Through Syk by Han Gyung Kim, Ji Hye Kim, Tao Yu, Jae Youl Cho

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Targeted Delivery of Nanovaccine to Dendritic Cells via DC-Binding Peptides Induces Potent Antiviral Immunity in vivo [Expression of Concern] by Lu Y, Liu ZH, Li YX, Xu HL, Fang WH, He F

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Prediction of drug-drug plasma protein binding interactions of resveratrol in combination with celecoxib and leflunomide by molecular docking combined with an ultrafiltration techn... by Zhou Peng, Hua Fang

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Antagonistic Trends Between Binding Affinity and Drug-Likeness in SARS-CoV-2 Mpro Inhibitors Revealed by Machine Learning by Anacleto Silva de Souza, Vitor Martins de Freitas Amorim, Eduardo Pereira Soares, Robson Francisco de Souza, Cristiane Rodrigues Guzzo

“…Notably, PK descriptors often exhibited opposing trends to binding affinity: hydrophilic features enhanced binding affinity but compromised PK properties, whereas hydrogen bonding, hydrophobic, and π–π interactions in Mpro subsites S2 and S3/S4 are fundamental for binding affinity. …”
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Ligand binding at the A-cluster in full-length or truncated acetyl-CoA synthase studied by X-ray absorption spectroscopy. by Peer Schrapers, Julia Ilina, Christina M Gregg, Stefan Mebs, Jae-Hun Jeoung, Holger Dau, Holger Dobbek, Michael Haumann

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The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcription by Igor Martianov, Stephanie Le Gras, Guillaume Davidson, Irwin Davidson

“…Summary: The multiprotein complex TFIID, comprising the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs), is an essential component of the RNA polymerase II (Pol II) preinitiation complex (PIC). …”
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