Antibody Aggregate Removal by Multimodal Chromatography by Veronika Rupčíková, Tomáš Molnár, Tomáš Kurák, Milan Polakovič

“…The review primarily assesses commercial multimodal resins in terms of aggregate removal efficiency, binding capacity, and scalability. Additionally, advancements in prototype resins and multimodal membranes are discussed. …”
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Covalent organic framework without cocatalyst loading for efficient photocatalytic sacrificial hydrogen production from water by Xuejiao Du, Haifeng Ji, Yang Xu, Shiwen Du, Zhaochi Feng, Beibei Dong, Ruihu Wang, Fuxiang Zhang

“…Abstract Metals are typically essential as either integral components within photocatalysts or as cocatalyst modifiers to enable efficient artificial photosynthesis, such as water splitting and carbon dioxide reduction. …”
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Synergistic optimizations of efficacy and membrane permeability of IRAK4 inhibitors: identifying new lead compounds for anti-inflammatory therapeutics by Kewon Kim, Ahyoung Jang, Hyeonsoo Han, Taeho Kim, Hwangseo Park, Sungwoo Hong

“…We initiated a drug discovery campaign based on the N2,N4-diphenylpyrimidine-2,4-diamine (DPDA) scaffold, employing an integrated strategy that combined structure-based de novo design, three-dimensional quantitative structure–activity relationship (3D-QSAR) modelling, and biochemical evaluation. …”
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Analysing protein complexes in plant science: insights and limitation with AlphaFold 3 by Pei-Yu Lin, Shiang-Chin Huang, Kuan-Lin Chen, Yu-Chun Huang, Chia-Yu Liao, Guan-Jun Lin, HueyTyng Lee, Pao-Yang Chen

“…It also discusses current limitations and offers perspectives on integrating molecular dynamics and experimental validation to enhance its capabilities.…”
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Swelling polymer-regulated green synthesis of flexible ZIF-8 membrane by Hongyu Ma, Ruoxin Wang, Kaiqiang He, Yaoxin Hu, George P. Simon, Huanting Wang

“…This membrane demonstrated good structural integrity and mechanical stability whilst achieving an H2 permeance of 6.81 ​× ​10−7 ​mol ​m−2 ​s−1·pa−1 and an ideal H2/CH4 selectivity of 24, which was able to retain its gas separation performance after 10 mild bends. …”
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Proteomics‐Empowered Microfluidic‐SERS Immunoassay for Identifying and Detecting Biomarkers of Micropapillary Lung Adenocarcinoma by Dechun Zhang, Kaiming Peng, Hui Xu, Yanping Chen, Jing Wang

“…To enable sensitive, multiplex detection of 4 upregulated DEPs, the nanomixing effect is leveraged to enhance target protein‐SERS barcode interactions while minimizing nonspecific binding to antibody‐functionalized gold electrodes. …”
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Structurally convergent antibodies derived from different vaccine strategies target the influenza virus HA anchor epitope with a subset of VH3 and VK3 genes by Ting-Hui Lin, Chang-Chun David Lee, Monica L. Fernández-Quintero, James A. Ferguson, Julianna Han, Xueyong Zhu, Wenli Yu, Jenna J. Guthmiller, Florian Krammer, Patrick C. Wilson, Andrew B. Ward, Ian A. Wilson

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Recent Advances in Enhancing the Sensitivity of Biosensors Based on Field Effect Transistors by Yuying Zhou, Tongshi Feng, Yao Li, Xiang Ao, Shengfa Liang, Xiaonan Yang, Lingfei Wang, Xiaoxin Xu, Wenchang Zhang

“…This review focuses on the current approaches from multiple perspectives, including overcoming the influence of Debye shielding effects, promoting the binding efficiency of analytes and FETs surface, and improving the structures of FETs device. …”
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Linked circadian outputs control elongation growth and flowering in response to photoperiod and temperature by Daniel D Seaton, Robert W Smith, Young Hun Song, Dana R MacGregor, Kelly Stewart, Gavin Steel, Julia Foreman, Steven Penfield, Takato Imaizumi, Andrew J Millar, Karen J Halliday

“…In flowering, the model predicted regulatory links from the clock to CYCLING DOF FACTOR 1 (CDF1) and FLAVIN‐BINDING, KELCH REPEAT, F‐BOX 1 (FKF1) transcription. …”
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Physiologically Based Pharmacokinetic Models for Infliximab, Ipilimumab, and Nivolumab Developed with GastroPlus to Predict Hepatic Concentrations by Celeste Vallejo, Cameron Meaney, Lara Clemens, Kyunghee Yang, Viera Lukacova, Haiying Zhou

“…<b>Methods:</b> The models include distribution and clearance mechanisms specific to large molecules, FcRn binding dynamics, and target-mediated drug disposition (TNF-α for infliximab, CTLA-4 for ipilimumab, and PD-1 for nivolumab). …”
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Conjoint analysis of methylation, transcriptomic, and proteomic profiles in pemphigus vulgaris by Xiaojia Luo, Jianting Ouyang, Fuqiong Jiang, Yaozhong Zhang, Yuan Wang, Yongzhuo Wu, Lingyu Hu

“…Abstract Background The underlying pathogenesis of pemphigus vulgaris, an autoimmune skin disorder, remains incompletely understood. An integrative analysis comprising DNA methylation, mRNA expression, and proteomic data in patients with pemphigus vulgaris was conducted to identify potential pathogenic contributors and explore the molecular mechanisms involved in its pathogenesis. …”
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Discovery of novel and highly potent dual-targeting PKMYT1/HDAC2 inhibitors for hepatocellular carcinoma through structure-based virtual screening and biological evaluation by Yang Yang, Yuting Wang, Jing Chen, Miao-Miao Niu, Yongbin Wang, Xing Jin

“…Molecular dynamics have confirmed the strong binding stability of PKHD-5 with PKMYT1 and HDAC2. …”
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From inflammation to immune regulation: The dual nature of dietary lectins in health and disease by Emadeldin H.E. Konozy, Makarim Elfadil M. Osman

“…However, they may also contain lectins, carbohydrate-binding proteins with potential health risks. While some lectins exhibit stability and resistance to digestion, posing threats to gastrointestinal integrity and immune function, others, such as those from butterfly peas and pink bauhinia, show immunomodulatory properties that could bolster immune responses. …”
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A Multi-Input Neural Network Model for Accurate MicroRNA Target Site Detection by Mohammad Mohebbi, Amirhossein Manzourolajdad, Ethan Bennett, Phillip Williams

“…However, it is challenging due to the high cost and time demands of experimental methods and the high false-positive rates of computational approaches. (2) Methods: We introduce a Multi-Input Neural Network (MINN) algorithm that integrates diverse biologically relevant features, including the microRNA duplex structure, substructures, minimum free energy, and base-pairing probabilities. …”
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Influence of carbon variation on the microstructure and hardness of CrTiAlCN coatings by Awais Akhtar, Anand Vyas, Haihui Ruan

“…The structural, morphological, surface chemical binding states, nanostructure, and nanohardness of the CrTiAlCN coatings were analyzed using microstructural and mechanical characterizations. …”
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Exploring bioactive phytoconstituents as USP21 inhibitors for therapeutic development against cancer by Saleha Anwar, Mohd Shahnawaz Khan, Dharmendra Kumar Yadav, Moyad Shahwan, Anas Shamsi

“…The selected compounds were subjected to physicochemical properties and binding affinity analysis for primary screening against USP21. …”
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Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription by Sandra Vergara, Xiaohong Zhou, Ulises Santiago, Mounia Alaoui-El-Azher, James F. Conway, Nicolas Sluis-Cremer, Guillermo Calero

“…In the reactant state, dATP binding to RT-template/primer involves a single Mg2+ (site B) inducing formation of a negatively charged pocket where a second floating Mg2+ can bind (site A). …”
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A Combined Study on Optimization, In Silico Modeling, and Genetic Modification of Large Scale Microbial Cellulase Production by Md. Raisul Islam Rabby, Zabed Bin Ahmed, Gobindo Kumar Paul, Nafisa Nusrat Chowdhury, Fatema Akter, Mamudul Hasan Razu, Pranab Karmaker, Mala Khan

“…As a result, this review provides insights into the interrelationship between the characteristics of optimizing different culture conditions, genetic modification, and in silico enzyme modeling for the production of cellulase enzymes, which may aid in the advancement of large-scale integrated enzyme manufacturing of substrate-specific enzymes.…”
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Deciphering functional landscape and clinical implications of enhancer RNAs in lung adenocarcinoma by Aike Li, Liguang Zhang, Xinsheng Du, Yi Dong, Yan Guo, Jiwei Zhao, Xiuli Fang, Pingping Lin

“…This study aimed to investigate the role of eRNAs in LUAD pathogenesis and evaluate their potential as diagnostic biomarkers and therapeutic targets. Through integrated bioinformatics and experimental approaches, we identified differentially expressed eRNAs associated with LUAD progression. …”
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PECAM-1 Leu125Val (rs688) Polymorphism and Diabetic Nephropathy in Caucasians with Type 2 Diabetes Mellitus by Matej Završnik, Stojan Kariž, Jana Makuc, Maja Šeruga, Ines Cilenšek, Daniel Petrovič

“…Platelet endothelial cell adhesion molecule-1 (PECAM-1) plays a key role in the transendothelial migration of circulating leukocytes during inflammation and in the maintenance of vascular endothelial integrity. We hypothesized that genetic variation in PECAM-1 gene could be associated with diabetic nephropathy (DN) and with the level of soluble PECAM-1 in Caucasians with type 2 diabetes mellitus (T2DM). …”
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