An evolutionarily conserved metabolite inhibits biofilm formation in Escherichia coli K-12 by Jingzhe Guo, Wilhelmina T Van De Ven, Aleksandra Skirycz, Venkatesh P. Thirumalaikumar, Liping Zeng, Quanqing Zhang, Gerd Ulrich Balcke, Alain Tissier, Katayoon Dehesh

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p-CuGaO2/β-Ga2O3 interfaces: A high-throughput approach for interface prediction and generation for power device applications by Chowdam Venkata Prasad, Jang Hyeok Park, Qui Thanh Hoai Ta, Kyong Jae Kim, Ho Jung Jeon, Muzaffar Ali Mir, Mohsin Raza, Nguyen Ngoc Tri, Honggyun Kim, Madani Labed, You Seung Rim

“…This study integrates a p-type copper gallium oxide (p-CuGaO2) interlayer to enhance the performance of β-Ga2O3-based power devices, addressing challenges in achieving reliable p-type doping. …”
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Structural basis for catalysis and selectivity of phospholipid synthesis by eukaryotic choline-phosphotransferase by Jacquelyn R. Roberts, Yasuhiro Horibata, Frank E. Kwarcinski, Vinson Lam, Ashleigh M. Raczkowski, Akane Hubbard, Betsy White, Hiroyuki Sugimoto, Gregory G. Tall, Melanie D. Ohi, Shoji Maeda

“…Structural and functional analysis of these binding-sites reveal the critical residues for the DAG acyl-chain preference and the choline/ethanolamine selectivity. …”
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Optogenetic storage and release of protein and mRNA in live cells and animals by Chaeyeon Lee, Jeonghye Yu, Jongpil Shin, Jihwan Yu, Yungyeong Heo, Moosung Lee, Daseuli Yu, YongKeun Park, Won Do Heo

“…Here, we introduce RELISR (Reversible Light-Induced Store and Release), an optogenetic condensate system that enables reversible storage and release of proteins or mRNAs. RELISR integrates multivalent scaffolds, optogenetic switches, and cargo-binding domains to trap cargo in the dark and release it upon blue-light exposure. …”
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Brown adipocyte exosome - derived C22:6 inhibits the IL-1β signaling pathway to alleviate rheumatoid arthritis by Rui Jiang, Rui Jiang, Rui Jiang, Yuanyuan Huang, Yuanyuan Huang, Rongcai Ye, Rongcai Ye, Yujian Zhang, Yujian Zhang, Meng Dong, Meng Dong, Hanlin Zhang, Hanlin Zhang, Ziyu Cheng, Ziyu Cheng, Zhi Zhang, Zhi Zhang, Jiaqi Zhang, Qiaoli Zhang, Gang Sun, Wanzhu Jin, Wanzhu Jin, Wanzhu Jin

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Dynamic and structural insights into allosteric regulation on MKP5 a dual-specificity phosphatase by Erin Skeens, Federica Maschietto, Ramu Manjula, Shanelle Shillingford, James Murphy, Elias J. Lolis, Victor S. Batista, Anton M. Bennett, George P. Lisi

“…Collectively, these results demonstrate critical roles for the allosteric site in coordinating both MKP5 catalysis and MAPK binding.…”
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GOT2: a moonlighting enzyme at the crossroads of cancer metabolism and theranostics by Junxi Hu, Qingwen Liu, Qinglin Ren, Wenbo He, Jiaqi Hou, Xiaolin Wang, Xiaolin Wang, Yusheng Shu, Yusheng Shu

“…Many classical metabolic enzymes, beyond their involvement in pathways like glycolysis and glutamine metabolism, also function as transcription factors, RNA-binding proteins, or signaling molecules. These dual roles are crucial in processes such as cancer metabolic reprogramming, immune evasion, and drug resistance. …”
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Quantitative Super‐Resolution Imaging of Molecular Tension by Seong Ho Kim, Adam B. Yasunaga, Hongyuan Zhang, Kevin D. Whitley, Isaac T. S. Li

“…The technique integrates a molecular‐beacon PAINT imager with a hairpin molecular tension probe, achieving a force quantification range of 9–30 pN and maintaining a spatial resolution of 30–120 nm in low and high‐density regions. …”
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Design, Synthesis, enzyme inhibitory activity and molecular docking studies of new 1,2,3-triazole linked nucleobases by Aditya Aggarwal, Chanchal Vashisth, Komal Bharti, Neera Raghav, Surender Kumar

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Molecular model of TFIIH recruitment to the transcription-coupled repair machinery by Tanmoy Paul, Chunli Yan, Jina Yu, Susan E. Tsutakawa, John A. Tainer, Dong Wang, Ivaylo Ivanov

“…Combining existing structural data with AlphaFold predictions, we build an integrative model of the initial TFIIH-bound TCR complex. …”
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Ligand efficacy shifts a nuclear receptor conformational ensemble between transcriptionally active and repressive states by Brian S. MacTavish, Di Zhu, Jinsai Shang, Qianzhen Shao, Yuanjun He, Zhongyue J. Yang, Theodore M. Kamenecka, Douglas J. Kojetin

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GramSeq-DTA: A Grammar-Based Drug–Target Affinity Prediction Approach Fusing Gene Expression Information by Kusal Debnath, Pratip Rana, Preetam Ghosh

“…This chemical perturbation information is processed, and a compact dataset is prepared, serving as the functional feature set of the drugs. By integrating the drug, gene, and target features in the model, our approach outperforms the current state-of-the-art DTA prediction models when validated on widely used DTA datasets (BindingDB, Davis, and KIBA). …”
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TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family by Philip Schmiege, Dawid Jaślan, Michael Fine, Nidish Ponath Sadanandan, Alexandra Hatton, Nadia Elghobashi-Meinhardt, Christian Grimm, Xiaochun Li

“…Abstract TRPML2 activity is critical for endolysosomal integrity and chemokine secretion, and can be modulated by various ligands. …”
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Investigation of Fructus sophorae extract’s therapeutic mechanism in atrophic vaginitis based on network pharmacology and experimental validation by Jiarui Zheng, Jiarui Zheng, Weiming Wang, Wenting Song, Yehao Zhang, Mingjiang Yao, Guangrui Wang, Zishan Huang, Feng Li, Junguo Ren, Li Lin, Xiaodi Fan, Jianxun Liu

“…Molecular docking analysis revealed strong binding affinity between FS components and the functional domains of EGFR, AKT1, and ESR1. …”
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Myocardial dysfunction caused by MyBPC3 P459fs mutation in hypertrophic cardiomyopathy: evidence from multi-omics approaches and super-resolution imaging by Yupeng Wu, Yupeng Wu, Yuzhu Zhang, Yuzhu Zhang, Qirui Zheng, Qirui Zheng, Qiyuan Wang, Qiyuan Wang, Xingyu Fang, Xingyu Fang, Zaihan Zhu, Zaihan Zhu, Jing Lu, Dandan Sun, Dandan Sun

“…Moreover, our metabolomic and proteomic data suggested several key components of mitochondrial membrane integrity, myocardial remodeling, myocardial energy metabolism, oxidative stress, inflammation, and actin binding capacity were significantly altered in response to P459fs mutation.ConclusionsThis investigation indicated myocardial dysfunction and myocardial fiber disarray in clinical HCMs with MyBPC3 P459fs mutation and added potential pathways underlying this. …”
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Unlocking the lipid code: SREBPs as key drivers in gastrointestinal tumour metabolism by Haowen Tang, Yuting Zhang, Danni Zhao, Minjie Guo, Xiao Yuan, Xu Wang

“…As central regulatory hubs for lipid metabolism, sterol regulatory element binding proteins (SREBPs) integrate dietary metabolic signals and carcinogenic stimuli through subtype-specific mechanisms, thereby promoting malignant tumour phenotypes. …”
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A framework for complex signal processing via synthetic biological operational amplifiers by Wenjun Cao, Lili Liu, Qingxu Sun, Yang Shan, Ye Chen

“…In this study, we introduce a framework that integrates orthogonal operational amplifiers (OAs) into standardized biological processes to enable efficient signal decomposition and amplification. …”
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In vitro mechanistic studies and potential health benefits of a standardized bilberry extract in low mood and cognitive enhancement by Mehtap Kara, Gozde Hasbal-Celikok, Jacob Wilson, Pilar Gómez-Serranillos, Tugba Yilmaz-Ozden, Ezgi Öztas, Gul Özhan, Özge Sultan Zengin, Marta Sánchez Gómez-Serranillos, Claudia Owsianik, Fazle Rabbani, Merve Tunç, Nazlı Arda, Nazia M. Memon, Ikram Ujjan, Kenny Hawkins, Justine Davis, Gabriel Wilson, Giovanna Petrangolini, Amjad Khan, Amjad Khan

“…Neuroprotection was investigated using SH-SY5Y cells exposed to H2O2, assessing cell viability (MTT), membrane integrity (LDH release), and BDNF expression. Cytotoxicity was determined through the MTT assay in SH-SY5Y cells. …”
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3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis by Marco Masera, Chiara Cicconetti, Francesca Ferrero, Salvatore Oliviero, Ivan Molineris

“…Building on the computational tool 3plex, we developed 3plex Web, an accessible platform that enhances the prediction of RNA:DNA triplexes by integrating interactive visualization, statistical evaluation, and user-friendly downstream analysis workflows. 3plex Web implements new features such as input randomization for statistical assessments, interactive profile plotting for triplex stability, and customizable DNA Binding Domain (DBD) selection. …”
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Exploring anti-SARS-CoV-2 natural products: dual-viral target inhibition by delphinidin and the anti-coronaviral efficacy of deapio platycodin D by Jiani Lu, Yan Tang, Hongtao Li, Saisai Tian, Xixiang Chen, Xueyue Song, Pengcheng Qin, Jianrong Xu, Haiyan Zhu, Liqiang Ni, Huarong Du, Weidong Zhang, Weihua Li, Lili Chen

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