Membrane-dependent dynamics and dual translocation mechanisms of ABCB4: Insights from molecular dynamics simulations by Veronica Crespi, Ágota Tóth, Angelika Janaszkiewicz, Thomas Falguières, Florent Di Meo

“…ABCB4 is an ATP-binding cassette transporter expressed at the canalicular membrane of hepatocytes and responsible for translocating phosphatidylcholine into bile. …”
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HMGB1 coordinates SASP‐related chromatin folding and RNA homeostasis on the path to senescence by Konstantinos Sofiadis, Natasa Josipovic, Milos Nikolic, Yulia Kargapolova, Nadine Übelmesser, Vassiliki Varamogianni‐Mamatsi, Anne Zirkel, Ioanna Papadionysiou, Gary Loughran, James Keane, Audrey Michel, Eduardo G Gusmao, Christian Becker, Janine Altmüller, Theodore Georgomanolis, Athanasia Mizi, Argyris Papantonis

“…To address this, we mapped HMGB1 binding genome‐wide in two primary cell lines. We integrated ChIP‐seq and Hi‐C with graph theory to uncover clustering of HMGB1‐marked topological domains that harbor genes involved in paracrine senescence. …”
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Design, Synthesis, and Antifungal Analysis of Pyrazoline Derivatives Against <i>Candida</i> Species: A Comprehensive <i>In Vitro</i> and <i>In Silico</i> Approach by Muhammad Rohim, Yuli Haryani, Neni Frimayanti, Fauzan Zein Muttaqin, Hilwan Yuda Teruna, Rudi Hendra

“…A robust association between binding energy and MIC substantiates the predictive use of computational data. …”
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Deep learning-based dipeptidyl peptidase IV inhibitor screening, experimental validation, and GaMD/LiGaMD analysis by Yi He, Yan Zhang, Minghao Liu, Jiaying Li, Wannan Li, Weiwei Han

“…Results In this study, a DPP4 inhibitor screening method was developed, integrating receptor-based ConPLex, ligand-based KPGT, and molecular docking to enhance screening accuracy. …”
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An in silico to in vivo approach identifies retinoid-X receptor activating tert-butylphenols used in food contact materials by Brenda J. Mengeling, Azhagiya Singam Ettayapuram Ramaprasad, Martyn T. Smith, Dania Turkieh, Nicole C. Kleinstreuer, Kamel Mansouri, Kathleen A. Durkin, Michele A. La Merrill, J. David Furlow

“…We tested six butylphenols with increasing bulk at C4 in vivo for their ability to disrupt thyroid hormone receptor (TR) signaling, using an integrated luciferase reporter driven by TR-RXR binding and quantifiable morphological changes in a Xenopus laevis precocious metamorphosis assay. …”
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A Short-Chain Analogue of Seminolipid: Synthesis and Inhibitory Effect on Mouse Fertilization by Seung Gee Lee, Leila Vahdati, Laura Morelli, Luigi Panza, Federica Compostella, Nongnuj Tanphaichitr

“…SGG liposomes have direct affinity for the zona pellucida (ZP), the egg extracellular matrix. SGG is also integral to the formation of sperm lipid rafts, which are platforms on the sperm surface for ZP binding. …”
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Elucidation of Expression Patterns and Functional Properties of Archaerhodopsin Derived from <i>Halorubrum</i> sp. Ejinoor by Luomeng Chao, Yuxia Yang

“…Through heterologous expression in <i>E. coli</i> BL21 (DE3) and integrative biophysical analyses, we demonstrate that <i>He</i>AR adopts a stable trimeric architecture (129 kDa) with detergent-binding characteristics mirroring bacteriorhodopsin (BR); however, it exhibits a 10 nm bathochromic spectral shift (λmax = 550 nm) and elevated proton affinity (Asp-95 pKa = 3.5 vs. …”
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Characterization of membrane potential dependency of mitochondrial Ca2+ uptake by an improved biophysical model of mitochondrial Ca2+ uniporter. by Ranjan K Pradhan, Feng Qi, Daniel A Beard, Ranjan K Dash

“…The model is critical in developing mechanistic, integrated models of mitochondrial bioenergetics and Ca(2+) handling.…”
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Characterization of the vitrification parameters for oviduct aggregates in Camelus dromedarius by Mohamed M. M. El-Sokary, Mohamed M. M. El-Sokary, Hamad A. Albreiki, Seham F. Shehata, Karima Gh. M. Mahmoud

“…Challenges in preserving the structural integrity and viability of camel oviductal aggregates remain a key critical gap during cryopreservation. …”
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The microtubule-severing enzyme spastin regulates spindle dynamics to promote chromosome segregation in Trypanosoma brucei by Thiago Souza Onofre, Qing Zhou, Ziyin Li

“…Knockdown of spastin impairs spindle integrity and disrupts chromosome alignment and segregation. …”
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Physical activity promotion in the early childcare setting: a content analysis of the federal-state-wide educational framework plans in Germany by Stefanie Do, Maike Till, Sven Messing, Peter Gelius, Mirko Brandes, Jonas D. Finger, Sylke Oberwöhrmann, Sarah Forberger, Antje Hebestreit

“…We aim to analyze the integration of physical activity in educational framework plans in Germany. …”
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The histological development of the fetal human inferior colliculus during the second trimester by Reetuparna Nanda, Mihail Bota, Jaikishan Jayakumar, Jaikishan Jayakumar, Suresh S, S. Lata, E. Harish Kumar, Chitra Srinivasan, Sudha Vasudevan, Kumutha Jayaraman, Mohanasankar Sivaprakasam, Mohanasankar Sivaprakasam, Mohanasankar Sivaprakasam, Richa Verma

“…The inferior colliculus (IC) is an important midbrain station of the auditory pathway, as well as an important hub of multisensory integration. The adult mammalian IC can be subdivided into three nuclei, with distinct cyto- and myeloarchitectonical profiles and distinct calcium binding proteins expression patterns. …”
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The role of ATF3 in precision medicine of brain arteriovenous malformation: based on endothelial cell proliferation by Jiwei Ma, Lei Meng, Jiyu Ji, Xiangyang Wang, Nan Wu, Lei Sun, Shupeng Zhao

“…With the rapid advancement of single-cell sequencing (scRNA-seq) and the integration of multi-omics data offers a novel perspective for precision medicine.MethodsWe first analyzed scRNA-seq data from the GEO database. …”
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Naphthalimide-polyamine conjugates: a promising avenue for targeted anticancer therapy by Zhiyong Tian, Luyao Tian, Chaojie Wang

“…Future research focuses on AI-driven polyamine enhancement, nanoplatforms that can cross the blood-brain barrier (such as Angiopep-2-functionalized Ti@FeAu), and non-apoptotic cell death mechanisms like pyroptosis. Through the integration of structural innovation and multi-mechanistic synergy, this research sets up a design framework for precision oncology, illustrated by AI-optimized polyamine chains and nanoplatforms capable of crossing the blood-brain barrier. …”
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Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus by A. L. Matveev, Ya. A. Khlusevich, I. K. Baykov, I. V.  Babkin, E. P. Goncharova, V. V. Morozova, N. V. Tikunova

“…To develop the producing strain, random integration of the plasmid containing the gene encoding the target protein into the genome of the host cells is commonly used. …”
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TRIM25‐Mediated INSIG1 Ubiquitination Promotes MASH Progression Through Reprogramming Lipid Metabolism by Hao Zhang, Xiangxu Kong, Wei Wang, Huaxin Zhou, Haoran Qu, Zhengyao Guan, Honglei Wu, Xiangyu Zhai, Bin Jin

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Profiling of fecal analytes as a potential biomarker in rheumatoid arthritis by Zhiyi Wang, Yujia Shi, Yachen Yang, Bangdong Gong, Jianmin Xie

“…BackgroundLoss of gut barrier integrity has been observed in rheumatoid arthritis (RA). …”
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Systematic identification of post-transcriptional regulatory modules by Matvei Khoroshkin, Andrey Buyan, Martin Dodel, Albertas Navickas, Johnny Yu, Fathima Trejo, Anthony Doty, Rithvik Baratam, Shaopu Zhou, Sean B. Lee, Tanvi Joshi, Kristle Garcia, Benedict Choi, Sohit Miglani, Vishvak Subramanyam, Hailey Modi, Christopher Carpenter, Daniel Markett, M. Ryan Corces, Faraz K. Mardakheh, Ivan V. Kulakovskiy, Hani Goodarzi

“…Abstract In our cells, a limited number of RNA binding proteins (RBPs) are responsible for all aspects of RNA metabolism across the entire transcriptome. …”
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Nanobiosensors for revolutionizing parasitic infections diagnosis: a critical review to improve global health with an update on future challenges prospect by Soheil Sadr, Ashkan Hajjafari, Alireza Sazmand, Cinzia Santucciu, Giovanna Masala, Mahdi Soroushianfar, Shakiba Nazemian, Abbas Rahdar, Sadanand Pandey, Moez Guettari, Hassan Borji

“…Regarding Schistosoma, graphene oxide (GO)-based nanobiosensors with a soluble egg antigen (SEA) binding, and for Taenia, metallic nanobiosensors can detect parasites’ biomarkers even at low concentrations. …”
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14C-Methylamine-glutaraldehyde conjugation as an alternative to iodination for protein labeling by Penelope L. Moore, Leonard H. Damelin, Tim J. Harrison

“…The radiolabeled α-2-macroglobulin was activated using established protocols and tested for functional integrity using competitive binding assays in the presence of recombinant receptor associated protein, an alternative ligand for the α-2-macroglobulin cellular receptor. …”
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