Generation of New Hairless Alleles by Genomic Engineering at the Hairless Locus in Drosophila melanogaster. by Heiko Praxenthaler, Thomas K Smylla, Anja C Nagel, Anette Preiss, Dieter Maier

“…To this end the endogenous H locus was replaced with an attP site by homologous recombination, serving as a landing platform for subsequent site directed integration of different H constructs. This way we generated a complete H knock out allele HattP, reintroduced a wild type H genomic and a cDNA-construct (Hgwt, Hcwt) as well as two constructs encoding H proteins defective of Su(H) binding (HLD, HiD). …”
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QKDTI A quantum kernel based machine learning model for drug target interaction prediction by Gundala Pallavi, Ali Altalbe, R. Prasanna Kumar

“…It used Quantum Support Vector Regression (QSVR) with quantum feature mapping that takes into account a quantum feature space for molecular descriptors and allows encoding molecular and protein features, improved predictions of binding affinities. To enhance the model to be more computationally feasible, integration of the Nystrom approximation into the model allows providing an efficient kernel approximation while reducing overhead expenses. …”
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The N-terminus of murine leukaemia virus p12 protein is required for mature core stability. by Darren J Wight, Virginie C Boucherit, Madushi Wanaguru, Efrat Elis, Elizabeth M A Hirst, Wilson Li, Marcelo Ehrlich, Eran Bacharach, Kate N Bishop

“…Altogether, our data imply that p12 stabilises the mature MLV core, preventing premature loss of capsid, and that this is mediated by direct binding of p12 to the capsid shell. In this manner, p12 is also retained in the pre-integration complex where it facilitates tethering to mitotic chromosomes. …”
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Extending the Schizosaccharomyces pombe molecular genetic toolbox. by Dorota Fennessy, Agnes Grallert, Andrea Krapp, Adisa Cokoja, Alan J Bridge, Janni Petersen, Avinash Patel, Victor A Tallada, Elvan Boke, Ben Hodgson, Viesturs Simanis, Iain M Hagan

“…We describe how a natMX6/rpl42+ cassette can be used for positive and negative selection for integration at a targeted locus. To facilitate the evaluation of the impact of a series of mutations on the function of a gene of interest we have generated three vector series that rely upon different selectable markers to direct the expression of tagged/untagged molecules from distinct genomic integration sites. pINTL and pINTK vectors use ura4+ selection to direct disruptive integration of leu1+ and lys1+ respectively, while pINTH vectors exploit nourseothricin resistance to detect the targeted disruption of a hygromycin B resistance conferring hphMX6 cassette that has been integrated on chromosome III. …”
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Nanobodies: From Discovery to AI-Driven Design by Haoran Zhu, Yu Ding

“…We then explore their structure–function relationship, emphasizing features like their single-domain architecture and long CDR3 loop that contribute to their binding versatility. Additionally, we examine the growing interest in multiepitope nanobodies, in which binding to different epitopes on the same antigen not only enhances neutralization and specificity but also allows these nanobodies to be used as controllable modules for precise antigen manipulation. …”
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Applications and challenges in designing VHH-based bispecific antibodies: leveraging machine learning solutions by Michael Mullin, James McClory, Winston Haynes, Justin Grace, Nathan Robertson, Gino van Heeke

“…The development of bispecific antibodies that bind at least two different targets relies on bringing together multiple binding domains with different binding properties and biophysical characteristics to produce a drug-like therapeutic. …”
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Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking by Barbora Kaščáková, Anna Koutská, Michaela Burdová, Petra Havlíčková, Ivana Kutá Smatanová

“…Protein crystallogenesis represents a key step in X‐ray crystallography studies that employ co‐crystallization and ligand soaking for investigating ligand binding to proteins. Co‐crystallization is a method that enables the precise determination of binding positions, although it necessitates a significant degree of optimization. …”
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Recurrent targeted genes of hepatitis B virus in the liver cancer genomes identified by a next-generation sequencing-based approach. by Dong Ding, Xiaoyan Lou, Dasong Hua, Wei Yu, Lisha Li, Jun Wang, Feng Gao, Na Zhao, Guoping Ren, Lanjuan Li, Biaoyang Lin

“…Finally, we identified 8 genes that were recurrent target genes by HBV integration including fibronectin 1 (FN1) and telomerase reverse transcriptase (TERT1), two known recurrent target genes, and additional novel target genes such as SMAD family member 5 (SMAD5), phosphatase and actin regulator 4 (PHACTR4), and RNA binding protein fox-1 homolog (C. elegans) 1 (RBFOX1). …”
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Recent Advances in Phthalocyanine-Based Hybrid Composites for Electrochemical Biosensors by Keshavananda Prabhu Channabasavana Hundi Puttaningaiah, Jaehyun Hur

“…Herein, we discuss the synthetic strategies, material properties, working mechanisms, and integration methods for designing electrochemical biosensors. …”
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HIV-1 protease and reverse transcriptase control the architecture of their nucleocapsid partner. by Gilles Mirambeau, Sébastien Lyonnais, Dominique Coulaud, Laurence Hameau, Sophie Lafosse, Josette Jeusset, Isabelle Borde, Michèle Reboud-Ravaux, Tobias Restle, Robert J Gorelick, Eric Le Cam

“…The HIV-1 nucleocapsid is formed during protease (PR)-directed viral maturation, and is transformed into pre-integration complexes following reverse transcription in the cytoplasm of the infected cell. …”
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Role of APOBEC3 in genetic diversity among endogenous murine leukemia viruses. by Patric Jern, Jonathan P Stoye, John M Coffin

“…In phylogenetic analyses, Pmvs and Mpmvs were monophyletic, whereas Xmvs were divided into several clades, implying a greater number of replication cycles between the integration events. Four distinct primer binding site types (Pro, Gln1, Gln2 and Thr) were dispersed within the phylogeny, indicating frequent mispriming. …”
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Structural bioinformatics approaches for predicting novel drug targets in hepatitis C virus proteins: a comprehensive analysis by Miao Qu, Mingzhu Gao, Xisheng Sang, Miao Yu, Zihe Guan, Weizhi Chang

“…The research focused on predicting binding sites, evaluating protein-ligand interactions, and assessing the therapeutic potential of identified targets. …”
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A perspective on the selection and design of nanozyme-based aptasensors for small molecules by Tanu Bhardwaj, Tarun Kumar Sharma

“…In these sensors, aptamers confer specificity to the target analyte, while nanozymes function as transducers, converting a binding event (the binding of the aptamer to its target) into a detectable signal. …”
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Holographic reconstruction of black hole spacetime: machine learning and entanglement entropy by Byoungjoon Ahn, Hyun-Sik Jeong, Keun-Young Kim, Kwan Yun

“…We find that the metrics for a tight-binding chain and the Gubser-Rocha model are similar. …”
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Irans full membership in the SCO:Economic and Security implications by Mohsen Shariatinia

“…In other areas of security and economics, cooperation is not binding and is a function of consensus among members.…”
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A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75. by Stephen Hare, Ming-Chieh Shun, Saumya Shree Gupta, Eugene Valkov, Alan Engelman, Peter Cherepanov

“…Lens epithelium derived growth factor (LEDGF), also known as PC4 and SFRS1 interacting protein 1 (PSIP1) and transcriptional co-activator p75, is the cellular binding partner of lentiviral integrase (IN) proteins. …”
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Advanced strategies for screening and identifying RNA-targeted small molecules: Bridging therapeutic potential and innovation by Zukela Ruzi, Daxiong Han, Kailibinuer Aierken

“…Complementary approaches, including structure-based drug design (SBDD) and fragment-based drug discovery (FBDD), have provided critical insights into RNA-ligand interactions, enabling rational optimization of candidate molecules. The integration of emerging technologies, such as RNA sequencing and chemical crosslinking, has further enabled transcriptome-wide mapping of interactions and predictive modeling of binding affinity. …”
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Against the clock to address plastic pollution: Critical challenges to elaborate a comprehensive and ambitious Plastic Treaty by Leandra R. Gonçalves, D. G. Webster, Natalia M. Grilli, Carla I. Elliff, Vitoria M. Scrich, Giovanna S. R. Lopes, Alexander Turra

“…Amidst uncertainties and power dynamics influencing state positions, this research identifies 10 critical, yet unresolved, points within the treaty negotiations (i.e., the scope of the treaty, equity and differentiation, involvement of non-state actors, integration with existing agreements, standards and specifications, trade implications, monitoring and reporting, responsibility and historical contributions, binding vs. non-binding commitments, and financing and technology transfer). …”
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Hydrogel Conjugation: Engineering of Hydrogels for Drug Delivery by Linh Dinh, Sung-Joo Hwang, Bingfang Yan

“…In addition, the integration of therapeutic agents with nucleic acid-based hydrogels can be leveraged for sequence-specific binding, representing a leap forward in biomaterials. …”
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Troubles with Cognitive Neuroscience by Gabriel Vacariu, Mihai Vacariu

“…In few words, we present the main actual problems of cognitive neuroscience: the binding problem, localization, differentiation–integration in the brain, the troubles created by the brain imaging, and optimism vs. skepticism in cognitive neuroscience. …”
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