Enhanced efficacy of immune checkpoint inhibitors by folate-targeted multifunctional drug through synergistic therapy inducing ferroptosis and immunogenic cell death in bladder can... by Yibo Shi, Guangrui Fan, Enguang Yang, Yuanfeng Zhang, Hui Ding, Junqiang Tian, Liang Cheng, Hanzhang Wang, Tianzhi Hao, Baodui Wang, Zhiping Wang

“…Results: DIFP-FA, by incorporating doxorubicin and indocyanine green, leverages specific binding to folate receptors for precise targeting and efficient internalization into BC cells. …”
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Strength and microstructural evaluation of sustainable geopolymer mortars using calcium carbonate sludge and fly ash as precursors by Menglim Hoy, Soe Thandar, Suksun Horpibulsuk, Avirut Chinkulkijniwat, Apichat Suddeepong, Apinun Buritatum, Teerasak Yaowarat, Teerat Tesanasin, Jitwadee Horpibulsuk, Mantana Julvorawong

“…Furthermore, this study uniquely integrates machine learning (XGBoost and SHAP analysis) to predict compressive strength and revealed that the sodium silicate-to-sodium hydroxide ratio was the most influential parameter, followed by NaOH molarity. …”
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Uremic toxin receptor NR1H3 contributes to hyperlipidemia- and chronic kidney disease-accelerated vascular inflammation, which is partially suppressed by novel YBX2 anti-ROS pathwa... by Yifan Lu, Yu Sun, Fatma Saaoud, Keman Xu, Ying Shao, Baosheng Han, Xiaohua Jiang, Laisel Martinez, Roberto I. Vazquez-Padron, Sadia Mohsin, Huaqing Zhao, Hong Wang, Xiaofeng Yang

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Reverse vaccinology and immunoinformatics approaches for multi-epitope vaccine design against Klebsiella pneumoniae reveal a novel vaccine target protein by Mayada M. Elfadil, Samah Omer A. Samhoon, Moaaz M. Saadaldin, Sabah A.E. Ibrahim, Ahmed Abdelghyoum M. Mohamed, Omnia H. Suliman, Osama Mohamed, Nadzirah Damiri, Mohd Firdaus-Raih, Sofia B. Mohamed, Qurashi. M. Ali

“…Molecular docking revealed high-affinity binding to Toll-like receptor 4 (TLR4) (−278.22 kcal/mol), stabilized by eight hydrogen bonds and two salt bridges. …”
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Plastic additive bisphenol S induces depression by promoting AZU1/CTSD proteins to mediate plasma-related proteins and metabolites: A comprehensive multi-omics analysis by Ni-ren Li, Pai Xie, Yi-xuan Zeng, Bing-ying Deng, Si-fan Lu, Yu-feng Gu, Yanhong Ma, Chao-hua Luo, Yi Liu

“…Molecular docking and molecular dynamics (MD) simulations were conducted to assess the binding stability between BPS and its target proteins. …”
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The antimicrobial peptide Cec4 has therapeutic potential against clinical carbapenem-resistant Klebsiella pneumoniae by Lu Li, Yang Zeng, Minfang Tian, Huijun Cao, Zhilang Qiu, Guo Guo, Feng Shen, Yuping Wang, Jian Peng

“…Its mechanism of action, such as destroying cell membranes and binding nucleic acid, has been revealed by various techniques, and its effectiveness has been confirmed in vivo, providing a promising candidate drug for combating CRKP infection.…”
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In silico and in vivo evaluations of fisetin and fisetin-loaded nanosuspension on monoamine oxidase inhibition in Aβ(25–35) induced dementia in mice model by Siti Zaidathul Iman Zolkiffly, Mizaton Hazizul Hasan, Siti Azma Jusoh, Ashok Kumar Janakiraman, Sathesh Kumar Sukumaran, Noreen Husain, Yuslina Zakaria, Hanish Singh Jayasingh Chellammal

“…Results: Fisetin docked to the catalytic positions of MAO-A and B, unveiling good binding scores and molecular interactions with amino acid residues for inhibition activities. …”
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Parp3 assists muscle function and skeletal muscle differentiation by selectively adjusting H3K27me3 enrichment by Zuleyha Yildirim, Aurélia Noll, Kathline Martin-Hernandez, Jean-Christophe Amé, Najat Hanini, Nadia Messaddeq, Isabelle Robert, Bernardo Reina San Martin, Gunn Hildrestrand, Magnar Bjoras, Françoise Dantzer

“…For some genes, Parp3 also helps the binding of Ezh2, the histone methyltransferase that catalyzes H3K27me3. …”
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Positive selection on rare variants of IGF1R and BRD4 underlying the cold adaptation of wild boar by Jianhai Chen, Ivan Jakovlić, Mikhail Sablin, Shengqian Xia, Zhixiang Xu, Yapin Guo, Renzuo Kuang, Jie Zhong, Yangying Jia, Nhien Thuy Thi Tran, Hao Yang, Hong Ma, Nikica Šprem, Jianlin Han, Di Liu, Yunxia Zhao, Shuhong Zhao

“…The H3K27ac CUT&Tag profiling revealed that the rs341219502 variant of IGF1R is linked to the gain of novel binding sites for three transcription factors involving regulatory changes in enhancer function. …”
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Deciphering the anticoccidial mechanism of Qinghao Changshan formula through network pharmacology and molecular docking by Dong Tian, Hui Fu, Hongxia Tao, Miaolan Li, Qinghua Zhang, Weidong Deng

“…This study aimed to elucidate the active components and molecular mechanisms of QHCS against coccidiosis using an integrated approach combining network pharmacology and molecular docking. …”
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Genotypic difference in response to copper stress in upland cotton as revealed by physiological and molecular expression analyses by Jianfei Wu, Tao Wang, Yin Huang, Shuiping Xiao, Xiaoxia Luo, Yanfeng Deng, Xiu Yang, Qingquan Kong, Feiyu Tang

“…Then both lines were subjected to a comprehensive comparative study, aiming to unravel the cotton Cu tolerance mechanism through integrated morphological, physio-biochemical, Cu uptake and distribution, and related molecular expression analyses. …”
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Case Report: Compound heterozygous variants in BHLHA9 cause complex syndactyly with oligodactyly, renal artery variation, and facial scar by Weidong Wei, Weidong Wei, Xiaosha Wang, Xiaosha Wang, Tao Zhang, Tao Zhang, Yongxing Zhong, Yongxing Zhong, Jintang Zhang, Jintang Zhang, Hua Yuan, Hua Yuan, Xiaoliang Shi, Xiaoliang Shi, Yao He, Yao He, Haitao Pan, Haitao Pan, Zhen Yang, Yuejuan Wang

“…The two variants all located within the helix-loop-helix (HLH) domain, a critical region for protein-protein interactions and DNA binding. Evolutionary conservation analysis revealed that the affected residues are highly conserved across species, and structural predictions suggested that the two variants may disrupt the HLH domain's structural integrity. …”
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Multivalent fusion protein targeting VEGFR2 and DR5 receptors: assessing the antiangiogenic and antitumor effects via multimodal microangiography by Irina N. Druzhkova, Anna G. Orlova, Anastasiia S. Fedulova, Arina V. Avakiants, Alina A. Isakova, Ekaterina V. Kukovyakina, Yuan Zijian, Ekaterina A. Plotnikova, Galina V. Trunova, Andrey A. Pankratov, Anton A. Plekhanov, Alexey A. Kurnikov, Pavel V. Subochev, Alexey K. Shaytan, Marine E. Gasparian, Mikhail P. Kirpichnikov, Dmitry A. Dolgikh, Daniel Razansky, Anne V. Yagolovich

“…Here, we investigated how the antiangiogenic activity of the SRH-DR5-B-iRGD fusion protein contributes to its antitumor effects. Methods An integrated approach has been applied involving molecular modeling of SRH-DR5-B-iRGD binding to DR5 receptor, optoacoustic (OA) and optical coherence tomography-based microangiography (OCT-MA) imaging of the vessel networks in xenografts of human glioblastoma and pancreatic adenocarcinoma in nude mice, supported by immunohistochemical (IHC) staining for vascularization marker CD31, and in vitro and in vivo bioactivity studies. …”
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The alteration of IL-17 signaling pathway in bipolar disorder: a preliminary study with transcriptomic perspective by Xiaobo Wang, Xiaobo Wang, Su Yu, Zhen Gao, Fangming Xu, Yumei Wang, Yumei Wang, Yumei Wang, Tianle Zhang, Tingting Xie, Xihua Jia

“…Enrichment analysis suggested that biological processes and signaling pathways related to extracellular matrix, stress response, heparin binding, and immune inflammation were upregulated in the BD group. …”
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NAP1L5 in acute myeloid leukemia: a prognostic biomarker and potential therapeutic target by Meng Wang, Meng Wang, Zhibin Xie, Yuanyuan Tan, Yan Zhou, Tingting Zhang, Yuqing Du, Huan Wu, Lili Zhou, Jian Ge

“…A predictive model integrating NAP1L5 expression and clinical AML features exhibited robust prognostic utility. …”
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Quantitative MODS-Wayne assay for rapid detection of pyrazinamide resistance in Mycobacterium tuberculosis from sputum samples by Emily Toscano-Guerra, Roberto Alcántara, Katherine Lozano Untiveros, Robert Gilman, Louis Grandjean, Mirko Zimic, Patricia Sheen

“…Notably, samples with mutations in the pyrazinamidase metal-binding site exhibited significantly lower POA levels compared with mutations in the enzyme periphery. …”
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The FKBPL-based therapeutic peptide, AD-01, protects the endothelium from hypoxia-induced damage by stabilising hypoxia inducible factor-α and inflammation by Sahar Ghorbanpour, Siân Peta Cartland, Hao Chen, Sanchit Seth, Rupert C. Ecker, Claire Richards, Dunja Aksentijevic, Matthew P. Padula, Louise Cole, Majid Ebrahimi Warkiani, Mary Meltem Kavurma, Lana McClements

“…This has impeded the development of effective therapies, particularly for peripheral artery disease. FK506-binding protein like (FKBPL) and its therapeutic peptide mimetic, AD-01, are crucial negative regulators of angiogenesis, however their roles in CVD are unknown. …”
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RBP7 knockdown inhibits proliferation of human hepatocellular carcinoma and activates the p38 MAPK pathway by Jinhai Li, Jinhai Li, Huawei Zhai, Fujing Cai, Xian Zhang, Xian Zhang, Yu Zhou, Shuqun Li, Shuqun Li, Huachun Song, Haifeng Zhang, Guangzheng Sun, Minghui Zhu, Jing Yuan, Ningxin Zhang, Ningxin Zhang, Maolin Yan, Maolin Yan

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Three Weeks on a Ketogenic Diet Reduces Free Testosterone and Free Estradiol in Middle-Aged Obese Men and Women by Mads Svart, Nikolaj Rittig, Thien Vinh Luong, Nigopan Gopalasingam, Esben Thyssen Vestergaard, Lars Gormsen, Esben Søndergaard, Henrik Holm Thomsen, Claus H. Gravholt

“…This investigation integrated samples from two distinct studies. Study I was a randomized, controlled, crossover trial with ten healthy, young male participants receiving either a weight-adjusted ketone ester or control (water, CTR) and vice versa following an overnight fast. …”
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CircSpna2 attenuates cuproptosis by mediating ubiquitin ligase Keap1 to regulate the Nrf2‐Atp7b signalling axis in depression after traumatic brain injury in a mouse model by Mengran Du, Jiayuanyuan Fu, Jie Zhang, Ziyu Zhu, Xuekang Huang, Weilin Tan, Lian Liu, Zhijian Huang, Xin Liu, Qiuhao Tan, ZhengBu Liao, Yuan Cheng

“…Mechanistically, circSpna2 was found to bind ubiquitin ligase Keap1, modulating the Nrf2‐Atp7b signaling pathway and influencing cuproptosis (docking score: −331.88). …”
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