Screening and characterization of the scFv for chimeric antigen receptor T cells targeting CEA-positive carcinoma by Chengcheng Zhang, Linling Wang, Qianzhen Zhang, Junjie Shen, Xia Huang, Meiling Wang, Yi Huang, Jun Chen, Yanmin Xu, Wenxu Zhao, Yanan Qi, Yunyan Li, Yanjiao Ou, Zhi Yang, Cheng Qian

“…Therefore, this study aimed to identify the optimal scFv and investigate its biological functions to further optimize the therapeutic potential of CAR-T cells targeting CEA-positive carcinoma.MethodsWe screened four reported humanized or fully human anti-CEA antibodies (M5A, hMN-14, BW431/26, and C2-45), and inserted them into a 3rd-generation CAR structure. …”
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Visualizing the enteric nervous system using genetically engineered double reporter mice: Comparison with immunofluorescence. by Yanfen Jiang, Hui Dong, Lars Eckmann, Elaine M Hanson, Katherine C Ihn, Ravinder K Mittal

“…<h4>Background and aims</h4>The enteric nervous system (ENS) plays a crucial role in the control of gastrointestinal motility, secretion and absorption functions. Immunohistochemistry has been widely used to visualize neurons of the ENS for more than two decades. …”
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Notch signaling in the tumor immune microenvironment of colorectal cancer: mechanisms and therapeutic opportunities by Jiachun Sun, Yi Chen, Ziyi Xu, Weizheng Wang, Penghui Li

“…Aberrant Notch signaling in CRC fosters immune evasion and tumor progression through its effects on the balance and functionality of immune cells, including myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs). …”
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A Highly Sensitive Silicon-Core Quantum Dot Fluorescent Probe for Vomitoxin Detection in Cereals by Caiwen Dong, Yaqin Li, Xincheng Sun, Xuehao Yang, Tao Wei

“…This toxin has strong immunosuppressive toxic effects and can cause serious damage to human and animal health. …”
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Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co₃O₄ Nanocatalyst by Changfeng Zhu, Yujiao Zhao, Jiyang Liu

“…Using interleukin-6 (IL-6) as a proof-of-concept demonstration, the epoxy functionalization of the SNF outer surface enabled the covalent immobilization of capture antibodies, forming a specific immunorecognition interface. …”
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Viral Mimetic Bacterial Outer Membrane Vesicles for Targeting Angiotensin-Converting Enzyme 2 by Ahn G, Yoon HW, Jeong JH, Kim YH, Shin WR, Song MS, Ahn JY

“…Gna Ahn,1,2,&ast; Hyo-Won Yoon,1,&ast; Ju Hwan Jeong,3,4,&ast; Yang-Hoon Kim,1,&ast; Woo-Ri Shin,1,5,6 Min-Suk Song,3 Ji-Young Ahn1 1Department of Microbiology, Chungbuk National University, Cheongju, Republic of Korea; 2Center for Ecology and Environmental Toxicology, Chungbuk National University, Cheongju, Republic of Korea; 3Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea; 4Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea; 5Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA; 6Department of Animal Bioscience & Integrated Biotechnology, Gyeongsang National University, Jinju, Republic of Korea&ast;These authors contributed equally to this workCorrespondence: Ji-Young Ahn; Min-Suk Song, Tel +82-43-261-2301 ; +82-43-261-3778, Email jyahn@chungbuk.ac.kr; songminsuk@chungbuk.ac.krPurpose: Outer membrane vesicles (OMVs) derived from Gram-negative bacteria naturally serve as a heterologous nano-engineering platform, functioning as effective multi-use nanovesicles for diagnostics, vaccines, and treatments against pathogens. …”
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Host Immune Response to Mycobacterium tuberculosis Infection: Implications for Vaccine Development by Liu Q, Que S, Qiu Y, Tang M, Liu S, Yang G, Wang Y, Deng A, Hu X, Lian X, Gao Q

“…We discuss how Mtb is recognized and phagocytosed by macrophages, how it evades immune responses, the recruitment and mobilization of neutrophils and monocytes, the role of natural killer cells during the infection process, how dendritic cells initiate adaptive immunity, the important roles of CD4+ T cells and their subtypes in TB infection, how CD8+ T cells exert cytotoxic functions, and how B cells produce antibodies and exhibit memory characteristics to eliminate pathogens. …”
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CCL3 correlates with ferroptosis in intervertebral disc degeneration and its prognostic significance by He Chen, Peng Xu, Leijing Mao, Yicong Wang, Zian Feng, Zhongxin Wang, Cailiang Shen, Yuanhong Xu

“…Despite its significant impact, effective diagnostic markers for early-stage IVDD remain elusive. …”
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CD28 shapes T cell receptor signaling by regulating Lck dynamics and ZAP70 activation by Kumarkrishna Raychaudhuri, Kumarkrishna Raychaudhuri, Rohita Rangu, Alison Ma, Neriah Alvinez, Andy D. Tran, Sandeep Pallikkuth, Katherine M. McIntire, Joseph A. Garvey, Jason Yi, Lawrence E. Samelson

“…We used anti-TCR (CD3) antibodies to trigger formation of TCR microclusters (MC), which are submicron-sized basic signaling units formed during T cell activation. …”
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CRISPR/Cas9-mediated PD-1 attenuation enhances tumor infiltrating lymphocyte-based adoptive cellular therapy in humanized-PDX model of hepatocellular carcinoma by Wu Ge, Huaping Liu, Di Wu, Yang Hu, Qi Liang, Muqi Liu, Hao Liu, Jianmin Wu, Juan Zhang, Yao Deng, Kedar Ghimire, Pengfei Rong, Wei Wang, Xiaoqian Ma

“…The phenotypic and functional changes were analyzed by flow cytometry. …”
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Reshaping the tumor microenvironment of cold soft-tissue sarcomas with anti-angiogenics: a phase 2 trial of regorafenib combined with avelumab by Maud Toulmonde, Jean-Philippe Guégan, Mariella Spalato-Ceruso, Thibaud Valentin, Rastilav Bahleda, Florent Peyraud, Christophe Rey, Michèle Kind, Coralie Cantarel, Carine Bellera, Lucile Vanhersecke, Alban Bessede, Antoine Italiano

“…Abstract The majority of sarcomas are under the influence of a tumor microenvironment that dampens immune activity, resulting in resistance to monoclonal antibodies targeting immune checkpoints and reduced clinical effectiveness. …”
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Trichuris trichiura egg extract proteome reveals potential diagnostic targets and immunomodulators. by Katalina Cruz, Antonio Marcilla, Patrick Kelly, Michel Vandenplas, Antonio Osuna, María Trelis

“…The accumulation of the long-term effects translates into poverty promoting sequelae and a cycle of impoverishment. …”
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Performance of Extracellular Vesicles From Leishmania (Leishmania) infantum for Serological Diagnosis of Human and Canine Visceral Leishmaniasis by Allecineia Bispo da Cruz, Francieli Marinho Carneiro, Noemi Nosomi Taniwaki, Gislene Mitsue Namiyama, Débora Oliveira dos Santos, Katia Gomes Castellão, Isabelle Martins Ribeiro Ferreira, Roberto Mitsuyoshi Hiramoto, Vera Lucia Pereira-Chioccola

“…(L.) infantum when used as antigen in ELISA identified the host antibodies. The methodology was effective for serological diagnosis of VL, since results exhibited good sensitivity and specificity for human and canine sera.…”
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Role of IL-17a in the Pathogenesis of Celiac Disease:  A Case–Control Study by Fatima Ali Khudair, Thanaa Shams Aldeen

“…Interleukin 17A (IL-17A) is a multifaceted pro inflammatory cytokine essential for vital functions such as host immunological defense, tissue healing, and the pathogenesis of inflammatory diseases. …”
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Changes in AXL and/or MITF melanoma subpopulations in patients receiving immunotherapy by M. Willemsen, J. Bulgarelli, S.K. Chauhan, R.R. Lereim, D. Angeli, G. Grisendi, G. Krebbers, I. Davidson, J.A. Kyte, M. Guidoboni, R.M. Luiten, W.J. Bakker

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Statin-Induced Necrotizing Autoimmune Myopathy: Case Report of a Patient under Chronic Treatment by Ilaria Anna Bellofatto, Marta Sessarego, Amedeo Tirandi, Chiara Olivero, Cosimo Sgura, Elia Maioli, Aurora Gavoci, Elisa Schiavetta, Federica Frè, Benedetta Saccomanno, Federico Zaottini, Riccardo Picasso, Chiara Fiorillo, Luca Liberale, Luciano Carlo Ottonello, Nicholas Bardi, Fabrizio Montecucco

“…He suffered from muscle functional impairment mainly involving proximal lower limb muscles which progressed to the point that he almost became bedridden. …”
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Fusion of SARS-CoV-2 neutralizing LCB1 peptide with Bacillus amyloliquefaciens RNase improves antiviral efficacy by Nikita N. Kostin, Tatyana V. Bobik, Elena V. Konovalova, Milita V. Kocharovskaya, Maria A. Simonova, Natalia Yu Rushkevich, Yuliana A. Mokrushina, George A. Skryabin, Roman S. Kalinin, Sergey I. Kovalchuk, Rustam H. Ziganshin, Leonid A. Kaluzhskiy, Oksana V. Gnedenko, Evgeniy O. Yablokov, Alexis S. Ivanov, Aleksandr S. Chernov, Vitaly A. Kazakov, Oksana N. Khokhlova, Arkady N. Murashev, Senlian Hong, Elena I. Shramova, Alexey A. Schulga, Ekaterina N. Lyukmanova, Mikhail P. Kirpichnikov, Ivan V. Smirnov, Alexey A. Belogurov, Yuri P. Rubtsov, Alexey V. Stepanov, Hongkai Zhang, Alexander G. Gabibov, Sergey M. Deyev

“…Abstract Virus-neutralizing peptides (VNPs) emerged as promising antiviral drug candidates with unprecedented specificity and cost-effectiveness during the recent COVID-19 pandemic. However, limited avidity, lack of effector functions, short circulatory half-life, and restricted administration routes make them inferior compared to neutralizing antibodies. …”
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Contextual Vulnerability Should Guide Fair Subject Selection in Xenotransplantation Clinical Trials by Gianna Strand

“…Clinician-investigators in contemporary literature repeatedly recommend that these trials enroll subjects who are medically eligible for, but effectively excluded or outright denied access to, an allograft.…”
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Prospects of therapeutic action on FGFR signaling pathway by M. Yu. Fedyanin, D. N. Khmelkova, T. S. Serebriyskaya, T. A. Nikolskaya, S. A. Tyulyandin

“…The most of these drugs are tyrosine kinase inhibitors and monoclonal antibodies. When we say about therapeutic effects on the FGFR signaling pathway, we say about opportunity not only block the ligands and FGFRs, but the under- lying molecular and signaling pathways activated by FGFRs. …”
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N-glycosylation of PD-L1 modulates the efficacy of immune checkpoint blockades targeting PD-L1 and PD-1 by Bar Kaufman, Muhammad Abu-Ahmad, Olga Radinsky, Eman Gharra, Tal Manko, Baisali Bhattacharya, Daniela Gologan, Nofar Erlichman, Tsipi Meshel, Yoav Nuta, Tomer Cooks, Moshe Elkabets, Adit Ben-Baruch, Angel Porgador

“…Results The binding of the PD-L1N35A mutant to PD-1 was not effectively blocked by anti-PD-L1 and anti-PD-1 ICBs. …”
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