Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats by Andreas V. Madsen, Peter Kristensen, Alexander K. Buell, Steffen Goletz

“…Bispecific antibodies (bsAbs) enable dual binding of different antigens with potential synergistic targeting effects and innovative therapeutic possibilities. …”
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AHR Agonist ITE Boosted PD1 Antibody’s Effects by Inhibiting Myeloid-Derived Cells Suppressive Cells in an Orthotopic Mouse Glioma Model by Pei Gong, Lijiao Zhao, Yunlong Ma, Qiuting Shu, Hui Sun, Jing Lu, Fanhua Meng, Fang Wan

“…AHR is known to mediate immune-suppressive functions of certain tryptophan metabolites such as kynurenine; yet, there lack of reports on how AHR agonists affect glioma immunity. …”
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The effect of pre-existing HLA sensitization on the renal allograft function according to angionephroscintigraphy results by O. G. Sinyakova, N. E. Kudryashova, E. V. Migunova, N. V. Borovkova, A. V. Pinchuk, N. V. Shmarina

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Effectiveness of benralizumab in the Tokyo Asthma Study (TOAST): A real-world prospective interventional trial by Katsunori Masaki, Maho Suzukawa, Hitoshi Sasano, Norihiro Harada, Yasunari Miyazaki, Hideki Katsura, Etsuko Tagaya, Junko Terada, Masayuki Hojo, Naoya Sugimoto, Hiroyuki Nagase, Yuta Kono, Hisato Hiranuma, Yasuhiro Gon, Ryo Takemura, Misato Irie, Reina Nakamura, Hiroki Kabata, Jun Miyata, Koichi Fukunaga

“…Background: Biologics are integral in the management of severe asthma. As the effectiveness of the anti-IL-5 receptor antibody benralizumab in Japan remains elusive, this study aimed to assess its real-world effectiveness in Japanese patients with severe asthma. …”
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Long‐term effects of obesity on COVID‐19 patients discharged from hospital by Luorui Shang, Li Wang, Fangyuan Zhou, Jinxiao Li, Yuhan Liu, Shenglan Yang

“…In addition, we found significant abnormalities in metabolic indicators such as blood lipids, uric acid, and liver function in obese patients. Most importantly, the antibody titer of COVID‐19 obese patients was inversely correlated with BMI. …”
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A humanized anti-MSLN×4-1BB bispecific antibody exhibits potent antitumour activity through 4-1BB signaling activation and fc function without systemic toxicity by Da-yan Zhang, Zhi-hua Zhang, Wen-ting Liu, Wei-ming Zhou, Peng-fei Zhou, Juan-juan Wei, Xue-jing Dai, Xiao-li Zeng, Yu-qiong Zhou, Han-wang Li, Heng Zhang, Ao-lin Shen, Lian-sheng Cheng, Guo-dong Shen, Yi-fu He

“…In particular, HK013-G1 retained Fc function and induced ADCC effect in tumour cells, whereas HK013-G4 did not. …”
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Applications of mutagenesis methods on affinity maturation of antibodies in vitro by LIU Yuan, LIN Manman, ZHANG Xiao, XU Chongxin, JIAO Linxia, ZHONG Jianfeng, WU Aihua, LIU Xianjin

“…As an essential determinant for antibody function for therapeutic and diagnostics use, a lots of antibodies, either isolated from antibody libraries or cloned from monoclonal antibodies, have been targeted to generate high affinity by directed evolution.Affinity maturation in vitro could mimic the mutation and selection process of affinity maturation in vivo, it could help to improve the affinity of the antibodies derived from antibody libraries and make the natural antibodies to break through affinity ceiling. …”
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Inhibitory Effect of Human Anti-CA I Autoantibodies and Development of Monoclonal Antibody mAb 2B8 Targeting Carbonic Anhydrase I by Petra Chalova, Barbora Jankovicova, Veronika Dvorakova, Eliska Zelinkova, Zuzana Bilkova, Marcela Slovakova, Lucie Korecka, Petr Muller, Maksym Danchenko, Lenka Minichova, Jan Lakota, Ludovit Skultety

“…Particularly, autoantibodies targeting CA I and II appear to induce cellular damage by inhibiting their respective protein’s catalytic functions. Our study illuminates the profound impact of anti-CA I autoantibodies from patient serum on the esterase activity of human CA I, exhibiting inhibitory effects akin to the acetazolamide inhibitor. …”
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CD94-driven in vitro expansion of highly functional adaptive NKG2C+ NKG2A- CD57+ NK cells from CMV+ healthy donors by Chiara Giordano, Simona Carlomagno, Simona Carlomagno, Michela Falco, Claudia Cantoni, Claudia Cantoni, Massimo Vitale, Ignazio Caruana, Johannes Dirks, Alberto Serio, Letizia Muccio, Giulia Bartalucci, Alessandra Bo, Franco Locatelli, Franco Locatelli, Cristina Bottino, Cristina Bottino, Simona Sivori, Simona Sivori, Mariella Della Chiesa, Mariella Della Chiesa

“…This new protocol for expanding functional adaptive NK cells is safe, cost-effective and easily implementable in a GMP context, suitable for innovative immunotherapeutic purposes.…”
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Antibody–Drug Conjugates (ADCs): current and future biopharmaceuticals by Ruili Wang, Baohui Hu, Ziyu Pan, Chongxia Mo, Xin Zhao, Guojia Liu, Ping Hou, Qi Cui, Zhao Xu, Wenjia Wang, Zhaojin Yu, Lin Zhao, Miao He, Yan Wang, Chen Fu, Minjie Wei, Lifeng Yu

“…This combination enables targeted delivery of cytotoxic agents to tumor site through recognizing target antigens by antibody while minimizing off-target effects on healthy tissues. …”
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Impact of IgG subclass on monoclonal antibody developability by Paul Cain, Lihua Huang, Yu Tang, Victor Anguiano, Yiqing Feng

“…Our results elucidated molecular attributes that are common to all IgG subclasses, as well as those that are unique to certain Fc domains, providing new insight into the effects of IgG subclass on antibody manufacturability and stability. …”
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Effects of Moringa oleifera leaf powder on production performance, immune function and antioxidant capacity of quails by WANG Zhigang, XU Chao, LI Xiahui, LIN Shiyu, DU Xiaxia, RAN Chonglin, SHU Gang

“…In order to study the effects of Moringa oleifera leaf powder on production performance, immune function and serum antioxidant indices of quails, different doses of M. oleifera leaf powder were added into basal diets. …”
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Evaluation of the effects of thyroid functions on frailty in geriatric patients using the Edmonton, SOF and FRAIL Scales by Galip Can Uyar, Mustafa Kemal Kılıç

“…While age-related chronic conditions have been extensively studied, the relationship between thyroid function and frailty remains underexplored. Objective This study aimed to evaluate the effects of thyroid dysfunction on frailty among individuals aged 65 years and older. …”
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Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…However, the frequent occurrence of a non-canonical disulfide bond between complementarity-determining region (CDR) H1 (C35a) and CDRH2 (C50) is often seen as a liability for therapeutic antibody development, despite limited reports of its effect on antibody binding, function, and stability. …”
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Effect of chemoradiotherapy on the dynamics of circulating lymphocyte subsets in patients with non-metastatic nasopharyngeal carcinoma by Lilan Yi, Lilan Yi, Yinfang Gu, Yinfang Gu, Longhua Guo, Longhua Guo, Xiaofang Zou, Xiaofang Zou, Guowu Wu, Guowu Wu

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Effect of Levothyroxine Replacement on Cognitive Function Impairment in a Sample of Egyptian Population with Subclinical Hypothyroidism by Salwa Seddik Hosny El-khwaga, Dina Ahmed Marwan, Nahla Nader Adly, Marina El-Amir Hakim, Ahmed Mohamed Bahaaeldin

“…Conclusion: SHT has a great effect on cognitive impairment, as normalization in TSH level results in improvement in cognitive function. …”
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Effect of SkQ1 Antioxidant on Structural and Functional Conditions of The Brain in PostResuscitation Period by M. L. Lovat, M. Sh. Avrushchenko, O. A. Averina, V. V. Pavshintsev, I. V. Ostrova, Y. V. Zarzhetsky, V. V. Moroz, M. V. Egorov

“…Data confirm the positive effects of SkQ1 on structural and functional status of the brain postre suscitation and suggest possible use of SkQ1 for the prevention or correction of posthypoxic encephalopathies.…”
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PCSK9-antibodies fail to block PCSK9-induced inflammation in macrophages and cannot recapitulate protective effects of PCSK9-deficiency in experimental myocardial infarction by Simon Rauterberg, Simon Rauterberg, Carmen Härdtner, Jennifer Hein, Paola Schrepf, Remi Peyronnet, Christoph Koentges, Tamara A. Vico, Carolin Ehlert, Bianca Dufner, Diana Lindner, Constantin von zur Mühlen, Dennis Wolf, Dirk Westermann, Ingo Hilgendorf, Alexander von Ehr

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Design of a fragment crystallizable-engineered tetravalent bispecific antibody targeting programmed cell death-1 and vascular endothelial growth factor with cooperative biological... by Tingting Zhong, Lingzhi Zhang, Zhaoliang Huang, Xinghua Pang, Chunshan Jin, Wenrong Liu, Juan Du, Wen Yin, Na Chen, Jing Min, Michelle Xia, Baiyong Li

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EFFECT OF POLYPHENOLIC COMPOUNDS ISOLATED FROM CARTHAMUS TINCTORIUS AND CALENDULA OFFICINALIS L., ON FUNCTIONAL ACTIVITY OF IMMUNE CELLS UNDER CONDITIONS OF CYTOSTATIC IMMUNOSUPPRE... by N. V. Masnaya, N. V. Isaikina, Ye. Yu. Sherstoboev, G. I. Kalinkina

“…The purpose of the study – to study the effect of polyphenolic compounds extracted from the flowers of safflower oil and calendula, the functional activity of immune cells in cytotoxic immune suppression.Conventional methods determined the total number of splenocytes, relative (%) and absolute (106), the number of antibody-forming cells (AFC) in the spleen of mice by local hemolysis by Cunningham.Evaluated the effect of compounds of natural origin on the cellular immune response in the delayed-type hypersensitivity (DTH). …”
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