Unraveling mitotic protein networks by 3D multiplexed epitope drug screening by Lorenz J Maier, Stefan M Kallenberger, Katharina Jechow, Marcel Waschow, Roland Eils, Christian Conrad

“…As a proof‐of‐concept, we mapped twelve epitopes in 3D‐cultured spheroids and investigated the network effects of twelve mitotic cancer drugs. Our approach reveals novel insights into spindle fragility and chromatin stress, and predicts unknown interactions between proteins in specific mitotic pathways. 3D SPECS's ability to map potential drug targets by multiplexed immunofluorescence in 3D cell culture combined with our automated high‐content assay will inspire future functional protein expression and drug assays.…”
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Transgenic overexpression of LARGE induces α-dystroglycan hyperglycosylation in skeletal and cardiac muscle. by Martin Brockington, Silvia Torelli, Paul S Sharp, Ke Liu, Sebahattin Cirak, Susan C Brown, Dominic J Wells, Francesco Muntoni

“…Overexpression of LARGE induces hyperglycosylation of α-dystroglycan in both wild type and in cells from dystroglycanopathy patients, irrespective of their primary gene defect, restoring functional glycosylation. Viral delivery of LARGE to skeletal muscle in animal models of dystroglycanopathy has identical effects in vivo, suggesting that the restoration of functional glycosylation could have therapeutic applications in these disorders. …”
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The clinical and pathogenetic value of Foxp3+ T regulatory cells in rheumatoid arthritis by A. S. Avdeeva, Yu. P. Rubtsov, D. T. Dyikanov, E. L. Nasonov

“…It is believed that a quantitative defect in CD4+CD25+Foxp3+CD127 regulatory cells is especially characteristic of early RA and associated with the risk of the latter in asymptomatic patients positive for anti-cyclic citrullinated peptide antibodies. The use of disease-modifying antirheumatic drugs and biologic agents is accompanied by a certain change in the level and functional activity of Tregs, which is responsible for the therapeutic effect of the medicaments.Thus, an important part is assigned to Tregs in the pathogenesis of autoimmune rheumatic diseases, RA in particular. …”
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The Overlapping Biology of Sepsis and Cancer and Therapeutic Implications by Amit Kumar Tripathi, Yogesh Srivastava

“…Anti-inflammatory therapies, such as IL-6 and TNF-α inhibitors, show promise in mitigating inflammation, while immune checkpoint inhibitors like anti-PD-1 and anti-CTLA-4 antibodies are being explored to restore immune function in sepsis and enhance antitumor immunity in cancer. …”
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Combination of GLP-1 Receptor Activation and Glucagon Blockage Promotes Pancreatic β-Cell Regeneration In Situ in Type 1 Diabetic Mice by Liangbiao Gu, Dandan Wang, Xiaona Cui, Tianjiao Wei, Kun Yang, Jin Yang, Rui Wei, Tianpei Hong

“…Liraglutide, a widely used antidiabetic glucagon-like peptide-1 (GLP-1) analog, has displayed diverse β-cell-protective effects in type 2 diabetic animals. Glucagon receptor (GCGR) monoclonal antibody (mAb), a preclinical agent that blocks glucagon pathway, can promote the recovery of functional β-cell mass in type 1 diabetic mice. …”
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Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. by Nalini Marino, Sam Illingworth, Prithvi Kodialbail, Ashvin Patel, Hugo Calderon, Rochelle Lear, Kerry D Fisher, Brian R Champion, Alice C N Brown

“…Functional antibody could be synthesised and secreted from infected tumour cells without impacting the activity of the virus particle in terms of oncolytic potency, manufacturing yields or selectivity for tumour cells. …”
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A Self‐Assembling LYTAC Mediates CTGF Degradation and Remodels Inflammatory Tumor Microenvironment for Triple‐Negative Breast Cancer Therapy by Jia‐Yi Lin, Ye Wu, Xiao‐Hui Liang, Min Tang, Xin Sun, Sheng‐Xin Lu, Jin‐Mei Jin, Xin Guo, Bei Wang, Hong‐Zhuan Chen, Wei‐Dong Zhang, Xin Luan

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Sera contributing to mycobacterial growth restriction in vitro display enhanced Fc-mediated phagocytosis by Krista E. van Meijgaarden, Patricia S. Grace, Wenjun Wang, Delia Goletti, Fabrizio Palmieri, Manfred Wuhrer, Tom H.M. Ottenhoff, Simone A. Joosten

“…Together, we demonstrate disease state independent direct effects of sera to mycobacterial growth control and antibody-FcR interactions modulating phagocytic capacity.…”
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Features of women’s reproductive dysfunctions associated with prolonged stressful situations by O. H. Horbatiuk, A. S. Shatkovska, A. P. Hryhorenko, O. V. Vaskiv, I. O. Bets, I. M. Kustovska, A. I. Petrash

“…The aim of the work was a comprehensive study of the hormonal function of pituitary gland, ovaries, thyroid gland and adrenal glands in women of reproductive age with various types of menstrual cycle disorders caused by a prolonged stressful situation. …”
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Humanized scFv Molecule Specific to an Extracellular Epitope of P2X4R as Therapy for Chronic Pain Management by Adinarayana Kunamneni, Karin N. Westlund

“…This study describes the humanization methodology and characterization of an effective non-opioid single-chain fragment variable (scFv) biologic that reverses pain-related behaviors, in this case by targeting P2X4. …”
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TNF-Like Weak Inducer of Apoptosis Aggravates Left Ventricular Dysfunction after Myocardial Infarction in Mice by Kai-Uwe Jarr, Sabine Eschricht, Linda C. Burkly, Michael Preusch, Hugo A. Katus, Norbert Frey, Emmanuel Chorianopoulos

“…Aim of the Study. To analyze the effects of sTWEAK on myocardial function and gene expression in response to experimental myocardial infarction in mice. …”
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Critical role of Oas1g and STAT1 pathways in neuroinflammation: insights for Alzheimer’s disease therapeutics by Zhixin Xie, Linxi Li, Weizhong Hou, Zhongxi Fan, Lifan Zeng, Limin He, Yunxiang Ji, Jingbai Zhang, Fangran Wang, Zhou Xing, Yezhong Wang, Yongyi Ye

“…Interferon responses and Interferon-stimulated genes (ISGs) significantly function in neuroinflammatory and neurodegenerative diseases involving AD. …”
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An analysis of real-life data of infants born to mothers with autoimmune thyroiditis: do they need to be followed-up? by Beatrice Righi, Nives Melli, Alessandra Cassio, Alessio Canovi, Francesco Leo, Chiara Sartori, Alessandra Polese, Rossana Colla, Alessandro De Fanti, Giancarlo Gargano, Maria Elisabeth Street

“…However, because of the possible negative effects of maternal anti-thyroid antibodies, we underline the importance of monitoring thyroid autoimmunity during pregnancy, including both anti-TG besides anti-TPO antibodies.…”
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Impact of Nintedanib on tumor angiogenesis and vascular normalization in a mouse model of colorectal cancer by Naita M. Wirsik, Jens M. Seeger, Thomas Schmidt, Frank Hilberg, Hans F. Fuchs, Christiane J. Bruns, Oliver Coutelle, Hamid Kashkar, Lars M. Schiffmann

“…Abstract Background For many solid types of cancer including colorectal cancer treatment with the VEGF antibody bevacizumab as anti-angiogenic treatment has become standard of care. …”
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Ileal mucosal epithelial cell proliferation in old mice and in mice with Alzheimer's disease by Dinić Jovana, Đorđević Isidora, Vuković David, Puškaš Nela

“…Introduction: The epithelial cell layer of the gastrointestinal tract (GIT) undergoes constant renewal initiated by the proliferation of cells in the crypts of Lieberkuhn. Changes in GIT function are often seen in elderly patients and patients with neurodegenerative diseases, such as Alzheimer's disease. …”
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Role of proinflammatory cytokines in Hashimoto's thyroiditis associated with psychiatric disorders by P. A. Sobolevskaia, A. N. Gvozdeckii, I. V. Kudryavtsev, V. A. Chereshnev, L. P. Сhurilov

“…In addition, the ability of a number of cytokines outside the CNS itself to act on vagal afferents and through them to convey information to the CNS, affecting its state and functions, has been proven. It is reasonable to assume that minimal fluctuations in cytokine levels may also affect the state and function of the CNS. …”
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Insight into the avidity–affinity relationship of the bivalent, pH-dependent interaction between IgG and FcRn by Johannes Reusch, Jan Terje Andersen, Ulrich Rant, Tilman Schlothauer

“…Thus, the methodology provides reliable kinetic rate parameters for both binding modes and their direct relationship as a function of pH. Also, it deciphers the potential effect of the variable Fab arms on FcRn binding, in which SPR has limitations. …”
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New Possibilities for Evaluating Markers of Alzheimer's Disease in Blood Serum by S. V. Vorobev, A. Yu. Plekhanov, I. K. Ternovykh, A. S. Usikov, A. А. Lebedev, A. N. Smirnov, N. M. Shmidt, M. S. Dunaevskiy, S. P. Lebedev

“…Results. Graphene functionalized with sulfo groups from pyranine exhibits sufficient ability to immobilize antibodies. …”
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Efficacy of Aflibercept (8 mg) for Diabetic Macular Edema in Vitrectomized Eyes Refractory to the Other Anti-VEGF Drug Therapies: A Report of Three Cases by Kashiwagi I, Nonaka R, Sasaki S, Yamamoto T, Yasuda K, Shimura M

“…In this case, brolucizumab, which has a high anti-VEGF molar concentration, and faricimab, which has a low anti-VEGF molar concentration but anti-angiopoietin (Ang)-2 activity, were not effective, but aflibercept (8 mg), whose VEGF molar concentration was intermediate between the two, was effective. …”
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Neutrophil extracellular traps impede cancer metastatic seeding via protease-activated receptor 2-mediated downregulation of phagocytic checkpoint CD24 by Yu Liu, Xiaoli Zhang, Kun Chen, Hongying Wang, Junhu Yuan, Yiming Ma, Jianhui Ma, Fanyu Zhang, Bing-zhi Wang, Yinong Wang, Xinhua Zhao

“…Furthermore, PAR2 blockade combined with an anti-EGFR antibody (cetuximab (CTX)) synergistically enhanced the phagocytic ability of macrophages and suppressed liver metastasis in vivo.Conclusions NET-derived elastase inactivated PAR2 canonical signaling and promoted phagocytosis by downregulating CD24, which functions as a phagocytotic checkpoint in CRC liver metastasis. …”
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