RUBY® – a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties by Barnabas Nyesiga, Mattias Levin, Anna Säll, Anna Rosén, Kim Jansson, Sara Fritzell, Karin Hägerbrand, Dietmar Weilguny, Laura von Schantz

“…Despite the large number of existing bispecific antibody (bsAb) formats, the generation of novel bsAbs is still associated with development and bioprocessing challenges. …”
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The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy by Xiu Li Feng, Man Man Zong, Guang Fang Zhou, Yang Zheng, Yuan Nan Yu, Rui Bing Cao, Pu Yan Chen, Mei Yang

“…The bursa of Fabricius is an acknowledged central humoral immune organ unique to birds, which is vital to B cell differentiation and antibody production. However, the function and mechanism of the biological active peptide isolated from bursa on B cell development and autophagy were less reported. …”
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A Targeted Integration-Based CHO Cell Platform for Simultaneous Antibody Display and Secretion by Jessica P. Z. Ng, Mariati Mariati, Jiawu Bi, Matthew Wook Chang, Yuansheng Yang

“…It provides a powerful and scalable tool for accelerating the development of functional, manufacturable therapeutic antibodies.…”
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Targeting Antibodies to Carbon Nanotube Field Effect Transistors by Pyrene Hydrazide Modification of Heavy Chain Carbohydrates by Steingrimur Stefansson, Hena H. Kwon, Saeyoung Nate Ahn

“…Many carbon nanotube field-effect transistor (CNT-FET) studies have used immobilized antibodies as the ligand binding moiety. …”
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An antibody-drug conjugate targeting soluble and membrane-bound TGFα is effective against pancreatic tumors by Inés Romero-Pérez, Juan Carlos Montero, Mónica Redondo-Puente, María del Carmen Gómez-García, Mireia Morell-Ginestà, Gabriel Capellá, Atanasio Pandiella

“…The therapeutic potential of targeting TGFα was evaluated through the development of two monoclonal antibodies (5F1 and 16B10) specific for TGFα. Results These antibodies effectively bound to proTGFα-expressing cells, with minimal off-target effects in TGFA-knockout cell lines. …”
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CoronaVac-induced antibodies that facilitate Fc-mediated neutrophil phagocytosis track with COVID-19 disease resolution by Chuang Li, Jie Yu, Rahma Issa, Lili Wang, Mingzhe Ning, Shengxia Yin, Jie Li, Chao Wu, Yuxin Chen

“…In addition to neutralization, antibodies can mediate various Fc-dependent effector functions, including antibody-dependent cellular phagocytosis (ADCP), antibody-dependent neutrophil phagocytosis (ADNP) and antibody-dependent cellular cytotoxicity (ADCC). …”
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Impact of Protein Kinase C Activation and Monoclonal Antibodies on Immune Checkpoint Regulation and B Cell Function in Patients with Chronic Lymphocytic Leukemia by Aviwe Ntsethe, Phiwayinkosi Vusi Dludla, Bongani Brian Nkambule

“…This study investigated B cell function in untreated patients with CLL, and we further explored the effects of ex vivo protein kinase C activation on immune checkpoint expression and B cell profiles. …”
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Discovery and functional characterization of canine PD-L1-targeted antibodies for evaluating antitumor efficacy in a canine osteosarcoma xenograft model by Min-young Song, Jaewon Cho, Hyosung Park, Yujeong Song, Keon Kim, Jae-Hee Ahn, Chang-Min Lee, Dae Hee Kim, Hyun-Jeong Ko

“…The clone #15 exhibited the highest binding affinity and most pronounced antitumor effects. The PD-1/PD-L1 interaction was inhibited by antibody #15. …”
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Emerging trends in synthesis, characterization, and mechanism of action of antibody-drug and antibody-nanoparticle conjugates by Tanu Dixit, Annamraju Aswini, Harshal Nikam, Anuradha Vaidya, Selvan Ravindran

“…This conjugation promotes controlled drug release, higher drug-to-antibody ratios (DAR), and reduced off-target effects. …”
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Immunotherapy against tau fragment diminishes AD pathology, improving synaptic function and cognition by Jie Xiang, Zhengjiang Qian, Ye Xi, Yanuo Wei, Guangxing Wang, Xia Liu, Zhi-Hao Wang, Zhentao Zhang, Shengxi Wu, Keqiang Ye

“…We conducted chronic intraperitoneal administration of the antibody to evaluate its effects on tau aggregation, synaptic integrity, and cognitive function. …”
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Broadly neutralizing antibodies isolated from HEV convalescents confer protective effects in human liver-chimeric mice by George Ssebyatika, Katja Dinkelborg, Luisa J. Ströh, Florian Hinte, Laura Corneillie, Lucas Hueffner, Elina M. Guzman, Prossie L. Nankya, Nina Plückebaum, Lukas Fehlau, Jonathan Garn, Nele Meyer, Sarah Prallet, Ann-Kathrin Mehnert, Anke R. M. Kraft, Lieven Verhoye, Carina Jacobsen, Eike Steinmann, Heiner Wedemeyer, Abel Viejo-Borbolla, Viet Loan Dao Thi, Thomas Pietschmann, Marc Lütgehetmann, Philip Meuleman, Maura Dandri, Thomas Krey, Patrick Behrendt

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Co-expression of anti-rotavirus proteins (llama VHH antibody fragments) in Lactobacillus: development and functionality of vectors containing two expression cassettes in tandem. by Gökçe Günaydın, Beatriz Alvarez, Yin Lin, Lennart Hammarström, Harold Marcotte

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Profound Functional Suppression of Tumor-Infiltrating T-Cells in Ovarian Cancer Patients Can Be Reversed Using PD-1-Blocking Antibodies or DARPin® Proteins by Emelie Foord, Charlotte Klynning, Esther Schoutrop, Judith M. Förster, Jennifer Krieg, Anette Mörtberg, Mischa R. Müller, Christel Herzog, Dieter Schiegg, Denis Villemagne, Ulrike Fiedler, Dan Snell, Benjamin Kebble, Jonas Mattsson, Victor Levitsky, Michael Uhlin

“…Our aim was to explore the enhancement of T-cell function by using novel PD-1-blocking proteins and compare with clinically approved monoclonal antibodies (mAbs). …”
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23ME-00610, a genetically informed, first-in-class antibody targeting CD200R1 to enhance antitumor T cell function by Jill Fenaux, Xin Fang, Yao-ming Huang, Cristina Melero, Caroline Bonnans, Earth Light Lowe, Tiziana Palumbo, Cecilia Lay, Zuoan Yi, Aileen Zhou, Mauro Poggio, Wei-Jen Chung, Sophia R. Majeed, Dylan Glatt, Alice Chen, Maike Schmidt, Clarissa C. Lee

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Novel cytokine–antibody fusion protein, N-820, to enhance the functions of ex vivo expanded natural killer cells against Burkitt lymphoma by John Lee, Dean Lee, Patrick Soon-Shiong, Yaya Chu, Gaurav Nayyar, Nang Kham Su, Jeremy M Rosenblum, Jeffrey T Safrit, Matthew Barth, Mitchell S Cairo

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Therapeutic effect of fully human anti-Nrp-1 antibody on non–small cell lung cancer in vivo and in vitro by Bo Zhang, Qin Liu, Lin Li, Yingchun Ye, Xiyuan Guo, Wenfeng Xu, Ligang Chen, Xianming Mo, Siji Nian, Qing Yuan

“…The aim of this study was to develop a fully human anti-Nrp-1 antibody with therapeutic effects against NSCLC in vitro and in vivo. …”
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Case Report: Therapeutic effect of efgartigimod in refractory anti-GQ1b antibody syndrome coexisting with myasthenia gravis by Keiko Watanabe, Seiya Takahashi, Akane Kanda, Takuya Watanabe, Yuki Kakinuma, Satoshi Yano, Ryuta Kinno, Ryuta Kinno

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A novel anti-NGF PEGylated Fab’ provides analgesia with lower risk of adverse effects by Yukari Koya, Hirotsugu Tanaka, Eiji Yoshimi, Nobuaki Takeshita, Shuji Morita, Hiroki Morio, Kanako Mori, Hiroshi Fushiki, Masazumi Kamohara

“…Nerve growth factor (NGF) has emerged as a key driver of pain perception in several chronic pain conditions, including osteoarthritis (OA), and plays an important role in the generation and survival of neurons. Although anti-NGF antibodies improve pain control and physical function in patients with clinical chronic pain conditions, anti-NGF IgGs are associated with safety concerns such as effects on fetal and postnatal development and the risk of rapidly progressive osteoarthritis. …”
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Patrolling monocytes mediate virus neutralizing IgG effector functions: beyond neutralization capacity by Abdelrahman Elwy, Swati Dhiman, Hossam Abdelrahman, Julia Specht, Theresa Charlotte Christ, Julia Falkenstein, Harpreet Kaur, Lisa Holnsteiner, Judith Lang, Matthias Mack, Falk Nimmerjahn, Wiebke Hansen, Karl Sebastian Lang

“…Identifying the types of effector cells engaged in antibody-mediated effector functions is essential for regulating their activities. …”
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TNFR2/CCR8 bispecific antibody enhances antitumor activity through depleting Ti-Tregs and boosting effector CD8+ T cell function by Ran Wang, Jiefang Xu, Shipeng Cheng, Zhiyang Ling, Wangmo Sonam, Jichao Yang, Fuquan Jin, Jing Wen, Xiao Lu, Liyan Ma, Yaguang Zhang, Xiaoyu Sun, Chunyan Yi, Bing Sun

“…Taken together, our findings suggest that precision depletion of Ti-Tregs via the bispecific TNFR2/CCR8 antibody is a potential therapeutic for cancer immunotherapy, while combination with anti-PD1 amplifies the antitumor effect.…”
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