FROM SEEING TO BELIEVING: A 43-YEAR LONG JOURNEY IN IMMUNOSTAINING

“… In the last 43 years, thanks to fantastic collaborators, we used antibodies in immunostaining and published a few firsts: TP53 in breast cancer (1988), FFPE-proofs anti Ki-67 antibody MIB 1 and the citrate antigen retrieval buffer (1992), reticular cells in human bone marrow (1993), BCL6 in germinal centers (1995), PRDM1 in plasma cells (2005), the sub cellular localization and distribution of AID and the function of IRF4 (2006). …”
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Thymol, Alpha‐Tocopherol and Ascorbyl Palmitate as a Natural Feed Supplements to Modulate Immune Variables and Heterophil to Lymphocyte Ratio in Broiler Chickens by Emiliano Ariel Videla, Jorge Martin Caliva, Romina Paula Andrea Picco, Raúl Héctor Marín, Agustin Luna, Franco Nicolas Nazar

“…However, their effects on immune function and stress responses in broilers require further investigation. …”
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Sympathetic nerves are sparsely distributed in rat mesenteric perivascular adipose tissue by William F. Jackson, Emma D. Flood, D. Adam Lauver, Gregory D. Fink, Stephanie W. Watts, Brian D. Gulbransen

“…Perivascular adipose tissue (PVAT) importantly affects the contractile function of conduit and resistance arteries. Some findings suggest that this effect of PVAT is controlled in part by sympathetic neural input directly to various PVAT depots. …”
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High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma by Xianlin Zeng, Xianlin Zeng, Xianlin Zeng, Xianhuai Jin, Xianhuai Jin, Ji Leng, Ji Leng, Ji Leng, Shuai Zhang, Yun Wang, Yun Wang, Yun Wang, Jin Chen, Jin Chen, Jin Chen, Shichao Zhang, Shichao Zhang, Shichao Zhang, Lijing Teng, Lijing Teng, Lijing Teng, Zuquan Hu, Zuquan Hu, Zuquan Hu, Shi Zhou, Zhu Zeng, Zhu Zeng, Zhu Zeng, Zhu Zeng, Zhu Zeng, Jinhua Long, Jinhua Long, Jinhua Long

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Vectorized Treg-depleting αCTLA-4 elicits antigen cross-presentation and CD8+ T cell immunity to reject ‘cold’ tumors by Laetitia Fend, Eric Quemeneur, Monika Semmrich, Jean-Baptiste Marchand, Matilda Rehn, Nathalie Silvestre, Linda Mårtensson, Johann Foloppe, Ingrid Teige, Björn Frendeus, Petra Holmkvist, Carolin Svensson, Christelle Remy, Patricia Kleinpeter, Jules Deforges, Fred Junghus, Kirstie L Cleary, Mimoza Bodén

“…Here we assess and identify spatially restricted exposure to a novel strongly Treg-depleting, checkpoint-blocking, vectorized αCTLA-4, as a highly efficacious and potentially safe strategy to target CTLA-4.Methods A novel human IgG1 CTLA-4 antibody (4-E03) was identified using function-first screening for monoclonal antibodies (mAbs) and targets associated with superior Treg-depleting activity. …”
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Pembrolizumab promotes degradation of cyclin dependent kinase 6 and suppresses ovarian cancer progression in vitro by Yi Wu, Ziyan Xu, Zuqiang Kou, Qiuling Ye, Liting Chen, Boyu Gou

“…Abstract Pembrolizumab is a novel humanized anti-PD-1 monoclonal antibody capable of enhancing T-cell mediated antitumor immunity. …”
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Steroid-Dependent Crohn’s Disease by William J Sandborn

“…Controlled ileal release budesonide, 6 mg/day, is an effective steroid-sparing agent, but it does result in some decrease in adrenal function. …”
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Astragalus polysaccharide enhances maternal mucosal immunity against PEDV by Jianli Tang, Shuaiyong Wang, Jianmei Tang, Jinming Li

“…Further analysis demonstrated that APS improved intestinal immune function and homeostasis in piglets. Overall, APS supplementation proved to be an effective immune booster, enhancing PEDV vaccine-induced mucosal immunity and providing a promising strategy for improving maternal immunity and piglet protection against PEDV.IMPORTANCEThis study highlights the limitations of current porcine epidemic diarrhea virus (PEDV) vaccines in inducing sufficient maternal milk IgA, which is crucial for protecting neonatal piglets. …”
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Photoelectrochemical immunosensing of amyloid-β peptide using nanoplasmonic macroporous bismuth vanadate for Alzheimer's disease diagnosis by Wenting Chen, Zhicheng Luo, Jiaxin Guo, Liang Li, Hao Wu

“…The sensing probe is composed of plasmonic Au nanoparticles (NPs) on the surface of 3DOM BiVO4 with the Aβ-specific antibody. Attributing to the nanoplasmonic effect and the periodic ordered porous structure, the Au NPs/3DOM BiVO4 photoelectrode shows a significant photocurrent response resulting from the strong light scattering effect and the enhanced separation of photoinduced charge carriers under simulated sunlight irradiation (AM 1.5G, 100 mW cm-2). …”
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Elevated plasma CXCL12 leads to pain chronicity via positive feedback upregulation of CXCL12/CXCR4 axis in pain synapses by Shi-Ze Leng, Mei-Jia Fang, Yi-Min Wang, Zhen-Jia Lin, Qian-Yi Li, Ya-Nan Xu, Chun-Lin Mai, Jun-Ya Wan, Yangyinhui Yu, Ming Wei, Ying Li, Yu-Fan Zheng, Kai-Lang Zhang, Ya-Juan Wang, Li-jun Zhou, Zhi Tan, Hui Zhang

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Novel insights into ORFV B2L DNA vaccine-mediated gut microbiota modulation and immune augmentation in rats by Umar Farooq, Umar Farooq, Guiqiong Liu, Guiqiong Liu, Sohail Ahmed, Sohail Ahmed, Huiguo Yang, Mehboob Ahmed, Xunping Jiang, Xunping Jiang

“…In sum, the results of the present study demonstrate that the ORFV B2L DNA vaccine (GV) elicited a more robust immune response and exerted a beneficial effect on composition and function of the gut microbiota compared with ORF live-attenuated vaccine. …”
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Allo-Priming Reverses Immunosenescence and May Restore Broad Respiratory Viral Protection and Vaccine Responsiveness to the Elderly: Results of a Phase I/II Clinical Trial by Canhui Liu, Xiaochuan Yang, Jorge Paoli-Bruno, David Sikes, Alejandra V. Marin-Ruiz, Nicole Thomas, Ryan Shane, Michael Har-Noy

“…Methods: A phase I/II, multi-center, open-label clinical trial was conducted in 40 healthy adults over 65 years of age to investigate the safety of allo-priming and the effects this vaccination strategy has on cellular immune function over time. …”
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Discovering Molecules That Regulate Efferocytosis Using Primary Human Macrophages and High Content Imaging. by Sandra Santulli-Marotto, Alexis Gervais, Jamie Fisher, Brandy Strake, Carol Anne Ogden, Chelsea Riveley, Jill Giles-Komar

“…Using high content imaging, we have developed a system for evaluating the effects of antibody treatment on apoptotic cell uptake in primary human macrophages by comparing the Phagocytic Index (PI) for each antibody. …”
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Macrophages generated in 3D mechanical microenvironment contribute to recruiting Peritonitis-associated neutrophils in vivo by YanRan Wang, XiaoHang Zou, YuQing Liu, GuoLin Shi, Xiang Li, Xiao Han, Pan Zhang, Hui Yang

“…Therefore, in this study, we aim to confirm the recruited effect of 3D-macrophage subsets on neutrophils during the immune process. …”
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Binding of CIB1 to the αIIb tail of αIIbβ3 is required for FAK recruitment and activation in platelets. by Meghna U Naik, Tejal U Naik, Ross Summer, Ulhas P Naik

“…<h4>Methods</h4>We used αIIb cytoplasmic domain peptide and CIB1-function blocking antibody to inhibit interaction of CIB1 with αIIb subunit as well as Cib1-/- platelets to evaluate the consequence of CIB1 interaction with αIIb on outside-in signaling.…”
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Influenza A virus N-linked glycosylation sites with very low glycan occupancy: identification, evolutionary dynamics, and impact on virus biology by Dr Irina Alymova, Dr Wen-Pin Tzeng, Dr Alexander Jureka, Dr John Barr, Dr Dongxia Wang, Dr Betlehem Mekonnen, Mr Shane Gansebom, Dr Ian York

“…Antibody responses generated by glycosylation mutant viruses were compared to those of the wild-type virus. …”
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Impact of Terminalia chebula Retz polysaccharide and Rubia cordifolia L. processed Terminalia chebula Retz polysaccharides on cyclophosphamide-induced immunosuppression in Chinese... by Jing Wang, Tiejin Tong, Qiang Wu, Min He

“…Plant-derived polysaccharides, such as those from Terminalia chebula Retz (TC) and Rubia cordifolia L. processed Terminalia chebula Retz polysaccharide (RTCP), offer promising alternatives due to their ability to enhance host defense mechanisms without adverse effects. This study investigates RTCP’s efficacy in mitigating cyclophosphamide (CTX)-induced immunosuppression in quails, focusing on immune function restoration.MethodsOne hundred and twenty 21-day-old healthy quails were randomly divided into six groups (n=20/group): a blank control (saline), a model group (saline + CTX), and four RTCP-treated groups (0.25, 0.5, 0.75, 1 g/kg + CTX). …”
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Evaluation of immune response to hepatitis B vaccine and its determinants among immunized anti-HBc-negative children in Ethiopia: A community-based cross-sectional study by Adane Adugna, Desalegn Abebaw, Mohammed Jemal

“…Conclusion: Childhood vaccination against hepatitis B is effective in only 65 % of children. In the study area, protective antibody levels (anti-HBs ≥10 mIU/ml) were reduced among immunized anti-HBc-negative children.…”
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IL-33/ST2 axis in diverse diseases: regulatory mechanisms and therapeutic potential by Feiya Sheng, Mi Li, Jia-Mei Yu, Si-Yu Yang, Liang Zou, Guan-Jun Yang, Le-Le Zhang, Le-Le Zhang

“…The mechanism of IL-33/ST2 in different diseases and its mediating effect on different immune cells have been summarized, as well as the antibody drugs targeting IL-33 or ST2, natural compounds with a mediating effect, and small molecule substances targeting relative pathway. …”
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Adrenergic Autoantibody‐Induced Postural Tachycardia Syndrome in Rabbits by Hongliang Li, Gege Zhang, Liping Zhou, Zachary Nuss, Marci Beel, Brendon Hines, Taylor Murphy, Jonathan Liles, Ling Zhang, David C. Kem, Xichun Yu

“…The heart rate response to infusion of the β‐adrenoceptor agonist isoproterenol was significantly enhanced in immunized animals, suggesting a positive allosteric effect of β1AR antibodies. In contrast, the blood pressure response to infusion of the α1‐adrenergic receptor agonist phenylephrine was attenuated in immunized animals, indicating a negative allosteric effect of α1‐adrenergic receptor antibodies. …”
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