Development and pharmacokinetic assessment of a fully canine anti-PD-1 monoclonal antibody for comparative translational research in dogs with spontaneous tumors by Sho Yoshimoto, Nicholas Chester, Ailian Xiong, Enrico Radaelli, Hong Wang, Marc Brillantes, Gayathri Gulendran, Patrick Glassman, Don L. Siegel, Nicola J. Mason

“…The lead scFv candidate re-formatted into a fully canine IgGD reversed the inhibitory effects of cPD-1:cPD-L1 interaction on canine chimeric antigen receptor (CAR) T cell function. …”
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Quercetagetin alleviates inflammatory osteoclastogenesis and collagen antibody-induced arthritis via Nrf2 signaling and Pten/AKT/Nfatc1 axis by Haojue Wang, Tao Yuan, Jingpeng Wang, Dengju Li, Wayne Yuk-wai Lee, Ziqing Li, Shui Sun

“…In vivo study further validated the therapeutic effect of Quercetagetin in collagen antibody-induced arthritis (CAIA) mice. …”
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CLEVER-1 targeting antibody, bexmarilimab, supports HLA-DR expression and alters ex vivo responsiveness to azacitidine and venetoclax in myeloid malignancies by Sofia Aakko, Arno Ylitalo, Heikki Kuusanmäki, Jenna H. Rannikko, Mari Björkman, Jami Mandelin, Caroline A. Heckman, Mika Kontro, Maija Hollmén

“…CLEVER-1 is a myeloid checkpoint protein, which can be targeted with a therapeutic function blocking antibody, bexmarilimab. Bexmarilimab has shown clinical efficacy in different solid tumors. …”
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Nanodesigner: resolving the complex-CDR interdependency with iterative refinement by Melissa Maria Rios Zertuche, Şenay Kafkas, Dominik Renn, Magnus Rueping, Robert Hoehndorf

“…Abstract Camelid heavy-chain only antibodies consist of two heavy chains and single variable domains (VHHs), which retain antigen-binding functionality even when isolated. …”
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A glyco-engineering approach for site-specific conjugation to Fab glycans by Maria L. Jaramillo, Traian Sulea, Yves Durocher, Mauro Acchione, Melissa J. Schur, Anna Robotham, John F. Kelly, Marie-France Goneau, Alma Robert, Yuneivy Cepero-Donates, Michel Gilbert

“…Effective processes for synthesizing antibody-drug conjugates (ADCs) require: 1) site-specific incorporation of the payload to avoid interference with binding to the target epitope, 2) optimal drug/antibody ratio to achieve sufficient potency while avoiding aggregation or solubility problems, and 3) a homogeneous product to facilitate approval by regulatory agencies. …”
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Plasma cell targeting with the anti-CD38 antibody daratumumab in myalgic encephalomyelitis/chronic fatigue syndrome—a clinical pilot study by Øystein Fluge, Øystein Fluge, Ingrid Gurvin Rekeland, Kari Sørland, Kine Alme, Kristin Risa, Ove Bruland, Karl Johan Tronstad, Olav Mella

“…Our working hypothesis is that ME/CFS in a subgroup of patients is associated with functional autoantibodies emerging after an infection, and that plasma-cell depletion with transient reductions in serum immunoglobulins will have a beneficial effect on patients’ symptoms.ObjectiveTo evaluate feasibility and toxicity of plasma-cell targeting treatment using the subcutaneous anti-CD38 antibody daratumumab (Darzalex®) in moderate to severe ME/CFS, and to assess the clinical course through 12–24 months follow-up after daratumumab intervention.MethodsWe performed a prospective, open-label pilot trial (EudraCT 2022–000281-18). …”
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Interplay of heavy chain introns influences efficient transcript splicing and affects product quality of recombinant biotherapeutic antibodies from CHO cells by Emma Kelsall, Claire Harris, Titash Sen, Diane Hatton, Sarah Dunn, Suzanne Gibson

“…Introns are included in genes encoding therapeutic proteins for their well-documented function of boosting expression. However, mis-splicing of introns in recombinant immunoglobulin (IgG) heavy chain (HC) transcripts can produce amino acid sequence product variants. …”
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A High-Affinity Monoclonal Antibody Against the Pancreatic Ductal Adenocarcinoma Target, Anterior Gradient-2 (AGR2/PDIA17) by Reeder M. Robinson, Leticia Reyes, Benjamin N. Christopher, Ravyn M. Duncan, Rachel A. Burge, Julie Siegel, Patrick Nasarre, Pingping Wang, John P. O’Bryan, G. Aaron Hobbs, Nancy Klauber-DeMore, Nathan G. Dolloff

“…Given the dire need for new therapeutic options for PDAC patients, we investigated the expression and function of AGR2 in PDAC and developed a novel series of affinity-matured AGR2-specific single-chain variable fragments (scFvs) and monoclonal antibodies. …”
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Tumor-associated bacteria activate PRDX1-driven glycolysis to promote immune evasion and PD-1 antibody resistance in hepatocellular carcinoma by Heng Zhang, Xi Lan, Liquan Cai, Xunfeng Gao, Feng Gao, Dan Yu, Jinlong Zhang, Jinhui Zhang, Qinwen Tai

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Development and Characterization of a Primary Ciliated Porcine Airway Model for the Evaluation of In Vitro Mucociliary Clearance and Mucosal Drug Delivery by Janik Martin, Veronika Neubauer, Rebecca Rittersberger, Simon Treitler, Patrick Kopp, Cemre Günday, Iman Shrimo, Annabelle Dabbars, Frank Rosenau, Akif Emre Türeli, Nazende Günday-Türeli, Oliver Haedicke-Peters, Katharina Schindowski

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Preclinical Evaluation of a B7-H3 Targeting Antibody Enhancing NK Cell-Mediated Cytotoxicity for Ovarian Cancer Treatment by Hagelstein I, Mattern S, Wang K, Haueisen YE, Greiner SM, Englisch A, Staebler A, Singer S, Lutz MS

“…The dismal prognosis of the disease necessitates the urgent development of novel therapies. Monoclonal antibodies (mAbs) have transformed cancer treatment, yet their effectiveness in ovarian cancer remains limited. …”
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Engineering chimeric human and mouse major histocompatibility complex (MHC) class I tetramers for the production of T-cell receptor (TCR) mimic antibodies. by Demin Li, Carol Bentley, Jenna Yates, Maryam Salimi, Jenny Greig, Sarah Wiblin, Tasneem Hassanali, Alison H Banham

“…Therapeutic monoclonal antibodies targeting cell surface or secreted antigens are among the most effective classes of novel immunotherapies. …”
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Improved Biological Impacts of Anti-EGFR Monoclonal Antibody in KRAS-Mutant Colorectal Cancer Cells by Silica-Coated Magnetic Nanoparticle Conjugation by Maedeh Yousefi, Hamed Farzi-Khajeh, Mostafa Akbarzadeh-Khiavi, Azam Safary, Khosro Adibkia

“…Methods: MNPs were synthesized and surface-coated with silica, followed by functionalization and stabilization with polyethylene glycol (PEG). …”
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Belatacept-based immunosuppression in heart transplant recipients: A single center experience by Wairimu Magua, PhD, MS, Maggie Wang, MD, MSc, Darlington Pobee, MD, Anna Morris, PhD, Alexis K. Okoh, MD, Emily M. Eichenberger, MD, MHS, Geeta M. Karadkhele, MS, Divya Gupta, MD, J. David Vega, MD, Christian P. Larsen, MD, PhD, Anne Van Beuningen, MD, Alanna A. Morris, MD MSc

“…Background: We aim to describe the effect of belatacept on de novo donor-specific antibodies (DSA) formation, rejection, and renal function in heart transplant recipients. …”
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Immunoregulatory function of Ganoderma spore oil in immunocompromised mice by YI Youjin, HU shun, XIONG Xingyao, LIU Dongbo, ZHONG Yingli, YI Chuanzhu

“…In addition, the Ganoderma spore oil had remarkable promoting effect on immune regulation of immunocompromised mice, including cell immunity, humoral immunity and non-specific immunity.These results indicate Ganoderma spore oil can stimulate both specific and non-specific immune function in mice, and enhance the immunoregulation functions of the immunocompromised mice according to the Chinese Theory Evaluating Procedure and Test Method of Health Foods.…”
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Anti‐PF4 antibody inhibit proliferation of lung cancer cells without TP53 mutation by inducing apoptosis and cell cycle arrest by Mengjia Qian, Zhihui Min, Yanxia Zhan, Lili Ji, Bijun Zhu, Miaomiao Zhang, Qi Shen, Pengcheng Xu, Hao Chen, Yunfeng Cheng

“…Methods Anti‐PF4 antibody was used to neutralise PF4 to regulate platelet function in the co‐culture of platelets and lung cancer cells lines of H1299 and A549. …”
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Assessing thyroid health: phenotypic age compared to chronological age by Dongyu Yang, Cihang Lu, Haonan Zhang, Xiaoguang Shi, Ying Sun, Ying Shao, Shuting Fan, Lijun Tian, Di Teng

“…Mediation analysis revealed that mean cell volume mediated 10% of the association between phenotypic age and overt hypothyroidism, while lymphocyte percentage exhibited a negative mediation effect (−26%) in the association between phenotypic age and subclinical hypothyroidism.DiscussionPhenotypic age better captures aging-related changes in thyroid function than chronological age and may serve as a useful biological aging marker in clinical endocrine research.…”
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C-terminal tagging impairs AGO2 function by Kunal M. Shah, Alex F. F. Crozier, Anika Assaraf, Muzjda Arya, Paul Grevitt, Faraz Mardakheh, Michael J. Plevin, Tyson V. Sharp

“…Affinity tagging and antibody-based methods can introduce artefacts, and both the N- and C-terminal domains of AGO2 are critical for its function. …”
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Utility of 131I-HLX58-Der for the Precision Treatment: Evaluation of a Preclinical Radio-Antibody-Drug-Conjugate Approach in Mouse Models by Liu Y, Wang X, Zhang N, He S, Zhang J, Xu X, Song S

“…The average tumor size in groups treated with 131I-HLX58-Der and HLX58-Der was reduced by factors of 12.15 and 4.80, respectively, compared to the control group. 131I-HLX58-Der demonstrated no toxic effects on hepatorenal function, routine blood tests, or major organs in mice when compared to the control group.Conclusion: These findings validate the potential of RADCs targeting CLDN18.2 in treating CLDN18.2-expressing solid tumors. …”
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GDF15 Neutralization Ameliorates Muscle Atrophy and Exercise Intolerance in a Mouse Model of Mitochondrial Myopathy by Stephen E. Flaherty III, LouJin Song, Bina Albuquerque, Anthony Rinaldi, Mary Piper, Dinesh Hirenallur Shanthappa, Xian Chen, John Stansfield, Shoh Asano, Evanthia Pashos, Trenton Thomas Ross, Srinath Jagarlapudi, Abdul Sheikh, Bei Zhang, Zhidan Wu

“…Previous studies have shown that GDF15 neutralization is effective in improving skeletal muscle mass and function in cancer cachexia. …”
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