Method for Printing Functional Protein Microarrays by James B. Delehanty, Frances S. Ligler

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Pre-Vaccination Immune Profiles and Responsiveness to Innate Stimuli Predict Reactogenicity and Antibody Magnitude Following mRNA Vaccination by Amanda E. Zelkoski, Emilie Goguet, Emily Samuels Darcey, Mohamad-Gabriel Alameh, Hooda Said, Simon Pollett, John H. Powers, Eric D. Laing, Cara Olsen, Edward Mitre, Allison M. W. Malloy

“…Background: While mRNA vaccines effectively limit hospitalization and severe COVID-19 disease, the precise early innate immune mechanisms associated with their efficacy and reactogenicity remain underexplored. …”
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Comprehensive analysis of nasal IgA antibodies induced by intranasal administration of the SARS-CoV-2 spike protein by Kentarou Waki, Hideki Tani, Eigo Kawahara, Yumiko Saga, Takahisa Shimada, Emiko Yamazaki, Seiichi Koike, Yoshitomo Morinaga, Masaharu Isobe, Nobuyuki Kurosawa

“…Our investigation is the first to demonstrate the function of nasal IgA at the monoclonal level, showing that nasal immunization can provide effective immunity against SARS-CoV-2 by inducing multimeric secretory IgAs at the target site of the virus infection.…”
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Selection of single chain antibody fragments binding to the extracellular domain of 4-1BB receptor by phage display technology by Salman Bagheri, Mehdi Yousefi, Elmira Safaie Qamsari, Farhad Riazi-Rad, Mohsen Abolhassani, Vahid Younesi, Ruhollah Dorostkar, Ali Akbar Movassaghpour, Zahra Sharifzadeh

“…Flow cytometry analysis demonstrated an acceptable specific binding of the single chain variable fragments to 4-1BB expressed on CCRF-CEM cells, while no binding was observed with an irrelevant antibody. Anti-4-1BB single chain variable fragments enhanced surface CD69 expression and interleukin-2 production in stimulated CCRF-CEM cells which confirmed the agonistic effect of the selected single chain variable fragments. …”
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Post‐COVID immunity in patients with solid tumor or hematological malignancies treated with SARS‐CoV‐2 monoclonal antibodies by Gilberto Sabino‐Santos, Cathryn E. Leggio, Sean M. Litwin, Najia Waheed, Shuangyi Bai, Sinem Ulusan, Anoli Karunathilake, Debra H. Elliott, Ashley R. Smira, Sruti Chandra, Lin Li, Bo Ning, Tony Hu, John S. Schieffelin, Bronwyn M. Gunn, James E. Robinson, Jyotsna Fuloria, Elizabeth B. Norton

“…Abstract Purpose SARS‐CoV‐2 monoclonal antibody (mAB) therapy has effectively treated severe COVID‐19, although how this contributes to protective antiviral immunity in settings of malignancy is poorly defined. …”
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Targeting the major pro-inflammatory interleukin-6-type cytokine receptor gp130 by antagonistic single domain antibodies by Silke Pudewell, Julia Heuser, Inna Dorogobed, Britta Lipinski, Thi Hong Hue Tran, Pia Metzenmacher, Richard Kunze, Felix Geyer, Stefan Zielonka, Doreen M. Floss, Harald Kolmar, Jens M. Moll, Jürgen Scheller

“…Furthermore, we functionally demonstrate the inhibitory effect of the selected nanobodies in cell-based transmigration assays of the human colorectal cancer cell line HT-29.DiscussionIn summary, our study has identified and characterized four novel inhibitory high-affinity gp130 nanobodies with potential for use in cytokine-dependent autoimmunity or cancer therapy.…”
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Engineered Bacterial Outer Membrane Vesicles Hitchhiking on Neutrophils for Antibody Drug Delivery to Enhance Postoperative Immune Checkpoint Therapy by Meng Guan, Xiao‐Ting Xie, Dong Zhou, Kai Cheng, Bin Zhang, Xin‐Yue Xu, Yong Li, Yi‐Tong Zhou, Wei Peng, Li‐Li Chen, Peng‐Shuo Dong, Si Chen, Jia‐Hua Zou, Bo Liu, Yuan‐Di Zhao, Jin‐Xuan Fan

“…Additionally, tumor cells overexpress the immune checkpoint CD47, further weakening the phagocytic and cytotoxic functions of macrophages. Here, the bacterial outer membrane vesicles (OMV) hitchhiking on neutrophils are utilized to precisely deliver immune checkpoint blockade antibodies to the tumor resection site. …”
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Novel fully human high-affinity anti-TREM2 antibody shows efficacy in clinically relevant Alzheimer´s mouse model by Markus Kraller, Julia Faßbender, Ammar Jabali, Joseph Kroeger, Barbara Fink, Bastian Popper, Martin Ungerer, Miriam A. Christlmeier

“…Additional safety investigations in mice showed no negative effects on blood cells or major organs. Conclusion Compared to existing humanized anti-hTREM2 antibodies that have been investigated in clinical trials, M07-TFN showed best-in-class affinities and agonist potencies. …”
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Fc-optimized CD276 antibody enhances NK cell activation against non-small cell lung cancer by Sylwia A. Stefańczyk, Sylwia A. Stefańczyk, Xenija Kaiser, Xenija Kaiser, Ilona Hagelstein, Ilona Hagelstein, Samuel Holzmayer, Samuel Holzmayer, Latifa Zekri, Latifa Zekri, Susanne Jung, Susanne Jung, Susanne Jung, Melanie Märklin, Melanie Märklin

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The Modulation of Fibrosis in Vocal Fold Repair: A Study on c-Met Agonistic Antibodies and Hepatocyte Growth in Animal Studies by Hyun-Il Shin, Hyunsu Choi, Jae-Kyun Jung, Choung-Soo Kim

“…This research examines the effectiveness of injections containing c-Met agonistic antibodies relative to HGF in reducing fibrosis within a rat model of vocal fold injury. …”
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Rapid Microfluidic Biosensor for Point-of-Care Determination of Rheumatoid Arthritis via Anti-Cyclic Citrullinated Peptide Antibody Detection by Wei-Yu Tai, To-Lin Chen, Hsing-Meng Wang, Lung-Ming Fu

“…The proposed microfluidic detection platform offers a fast and effective method for accurately identifying and quantifying the anti-CCP Ab biomarker. …”
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Analysis of the serum proteome profile of wild stump-tailed macaques (Macaca arctoides) seropositive for Zika virus antibodies in Thailand by Pakorn Ruengket, Sittiruk Roytrakul, Daraka Tongthainan, Kobporn Boonnak, Kanokwan Taruyanon, Bencharong Sangkharak, Wirasak Fungfuang

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High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand function... by Xiaoyue Shang, Nina Bartels, Johann Moritz Weck, Sabine Suppmann, Jérôme Basquin, Gajen Thaventhiran, Amelie Heuer-Jungemann, Cornelia Monzel

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Antithyroglobulin and Antiperoxidase Antibodies Can Negatively Influence Pregnancy Outcomes by Disturbing the Placentation Process and Triggering an Imbalance in Placental Angiogen... by Kamila Tańska, Piotr Glinicki, Beata Rebizant, Piotr Dudek, Wojciech Zgliczyński, Małgorzata Gietka-Czernel

“…However, several detrimental effects of thyroid antibodies were observed. These included a positive correlation between TgAb and the sEng/PlGF ratio in the first trimester and positive correlations between TPOAb and sFlt-1 and between TgAb and the sFlt-1/PlGF ratio in the third trimester. …”
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The effect of tofacitinib on function and quality of life indicators in patients with rheumatoid arthritis resistant to synthetic and biological disease-modifying antirheumatic dru... by D. E. Karateev, D. E. Abdulganieva, A. R. Babaeva, A. A. Baranov, L. P. Evstigneeva, O. N. Ivanova, G. V. Lukina, E. L. Luchikhina, V. I. Mazurov, A. S. Misiyuk, O. V. Semagina, A. E. Sizikov, V. N. Sorotskaya

“…The results of our multicenter study using considerable Russian material confirmed the pronounced positive effect of TOFA used as a second-line agent (after s-DMARD failure) and a third-line agent (after s-DMARD and BA failure) on patients' assessment of disease activity, functional ability in daily life, and quality of life.…”
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Unexpected PD‐L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies by Tanmoy Mondal, Gururaj N Shivange, Rachisan GT Tihagam, Evan Lyerly, Michael Battista, Divpriya Talwar, Roxanna Mosavian, Karol Urbanek, Narmeen S Rashid, J Chuck Harrell, Paula D Bos, Edward B Stelow, M Sharon Stack, Sanchita Bhatnagar, Jogender Tushir‐Singh

“…Abstract Lack of effective immune infiltration represents a significant barrier to immunotherapy in solid tumors. …”
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Clinical Benefits and Risks of Antiamyloid Antibodies in Sporadic Alzheimer Disease: Systematic Review and Network Meta-Analysis With a Web Application by Danko Jeremic, Juan D Navarro-Lopez, Lydia Jimenez-Diaz

“…ResultsAmong 7 treatments tested in 21,236 patients (26 studies with low risk of bias or with some concerns), donanemab was the best-ranked treatment on cognitive and functional measures, and it was almost 2 times more effective than aducanumab and lecanemab and significantly more beneficial than other treatments on the global (cognitive and functional) Clinical Dementia Rating Scale-Sum of Boxes (NNT=10, 95% CI 8-16). …”
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Immunoregulatory functions of immune complexes in vaccine and therapy by Yu‐mei Wen, Libing Mu, Yan Shi

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Nanobody-enhanced chimeric antigen receptor T-cell therapy: overcoming barriers in solid tumors with VHH and VNAR-based constructs by Shasha Guo, Xiaozhi Xi

“…While CAR-T therapy has effectively treated various blood cancers, it faces significant challenges in addressing solid tumors. …”
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Long-term outcomes of dupilumab therapy in severe asthma: A retrospective, multicenter, real-world study by Carlo Mümmler, MD, Alexandra Lenoir, MD, Jeremias Götschke, MD, Michael Gerckens, MD, Merle Kaiser, MD, Moritz Kayser, MD, Nora Drick, MD, Hendrik Suhling, MD, Leonie Biener, MD, Carmen Pizarro, MD, Dirk Skowasch, MD, Nikolaus Kneidinger, MD, PhD, Jürgen Behr, MD, Katrin Milger, MD

“…Conclusions: Dupilumab represents an effective long-term treatment option for patients with severe asthma, with sustained treatment effects up to 36 months. …”
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