IL1RAP Expression in Human Atherosclerosis: A Target of Novel Antibodies to Reduce Vascular Inflammation and Adhesion by Madelene Lindkvist, Anna Göthlin Eremo, Geena Varghese Paramel, Sheikh Anisul Haque, Camilla Rydberg Millrud, Sara Rattik, Caitríona Grönberg, David Liberg, Allan Sirsjö, Karin Fransén

“…The functional effect of IL1RAP antibodies on endothelial cells were analyzed with adhesion and permeability assays. …”
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Deciphering cross-species reactivity of LAMP-1 antibodies using deep mutational epitope mapping and AlphaFold by Tiphanie Pruvost, Magali Mathieu, Steven Dubois, Bernard Maillère, Emmanuelle Vigne, Hervé Nozach

“…We used deep mutational scanning (DMS) of the human LAMP-1 antigen displayed on yeast surface and leveraged next-generation sequencing to observe the effect of individual mutants on the binding of two LAMP-1 antibodies and to determine their functional epitopes on LAMP-1. …”
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Juxtamembrane shedding of Plasmodium falciparum AMA1 is sequence independent and essential, and helps evade invasion-inhibitory antibodies. by Anna Olivieri, Christine R Collins, Fiona Hackett, Chrislaine Withers-Martinez, Joshua Marshall, Helen R Flynn, J Mark Skehel, Michael J Blackman

“…Apical membrane antigen-1 (AMA1) is a merozoite protein that plays a critical role in invasion. Antibodies against AMA1 prevent invasion and can protect against malaria in vivo, so AMA1 is of interest as a malaria vaccine candidate. …”
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Generation of Site‐Specifically Labeled Affinity Reagents via Use of a Self‐Labeling Single Domain Antibody by Stanley Fayn, Swarnali Roy, Chino C. Cabalteja, Woonghee Lee, Hima Makala, Kwamena Baidoo, Divya Nambiar, Julia Sheehan‐Klenk, Joon‐Yong Chung, Jesse Buffington, Mitchell Ho, Freddy E. Escorcia, Ross W. Cheloha

“…These effects are particularly pronounced in studies using small antibody fragments, such as single‐domain antibodies, or nanobodies. …”
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A New Antibody–Cytokine Construct Targeting Natural Killer Cells: An Immunotherapeutic Approach to Chronic Lymphocytic Leukemia by Michela Flego, Mauro Andreotti, Francesca Romana Mauro, Maria Beatrice Arasi, Silvia Zamboni, Zuleika Michelini, Sara Pepe, Clementina Maria Galluzzo, Roberta Amici, Diego Moricoli, Chiara Mazzei, Alessandro Ascione, Alessandra Mallano

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Neutralising and Non-neutralising Antibodies to SARS-CoV-2: Role during Infection and in the Evolution of Antigenic structure by S. K. Pylaeva, A. A. Sinyugina, L. I. Kozlovskaya, E. A. Artamonova, A. A. Erovichenkov, R. F. Sayfullin, I. V. Gordeychuk, A. A Ishmukhametov

“…To examine the specifics of antibody formation, as well as the spectrum and functional activity of these antibodies in patients with COVID-19.Conclusions. …”
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Affinity-controlled capture and release of engineered monoclonal antibodies by macroporous dextran hydrogels using coiled-coil interactions by Seyed Farzad Baniahmad, Romane Oliverio, Ines Obregon-Gomez, Alma Robert, Anne E.G. Lenferink, Elena Pazos, Nick Virgilio, Xavier Banquy, Gregory De Crescenzo, Yves Durocher

“…Long-term delivery is a successful strategy used to reduce the adverse effects of monoclonal antibody (mAb)-based treatments. …”
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Monoclonal antibodies: their place in applied medicine and their role in the newest infectious disease – history, present, and future. Literature review by Petar Yordanov Atanasov, Maria Georgieva Moneva-Sakelarieva, Yozlem Ali Kobakova, Stefka Achkova Ivanova, Maria Vakrilova Becheva, Angelina Georgieva Kirkova-Bogdanova, Maria Sevdelinova Chaneva, Ventseslava Petrova Atanasova, Radoslav Fedeev Todorov, Nikolay Zarkov Bashev, Ismail Elhanov Bashov, Adel Habib Ibrahim

“…Efforts are being directed not only at improving the structural and functional properties of existing monoclonal antibodies but also at creating new types of antibodies with smaller molecular weights and higher specificity. …”
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A broadly neutralizing antibody recognizes a unique epitope with a signature motif common across coronaviruses by Lei Yan, Fulian Wang, Michelle Hill, Juliane Brun, Ze Liang, Xinyu Shi, Liangminghui Zhang, Xiuxiu He, Yu Li, Qianping Huang, Xuxue Dong, Huanzhen Liu, Yi Zhang, Lili Liu, Raymond A. Dwek, Nicole Zitzmann, Aibin Liang, Guang Yang

“…Notably, this cryptic epitope reveals a signature motif that extends throughout the core region of coronaviruses and is also present in various RNA viruses, including HIV and Marburgvirus. 3D1 functions as a natural or background antibody capable of binding to a diverse array of non-self antigens. 3D1’s cross-reactivity underscores the effectiveness of the library approach, which encompasses the entire antibody repertoire.…”
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CLN-619, a MICA/B monoclonal antibody that promotes innate immune cell-mediated antitumor activity by Naveen K Mehta, Kristan Meetze, Patrick A Baeuerle, Jennifer S Michaelson, Kerry A Whalen, Kavya Rakhra, Catherine C Henry, Safak Yalcin, Neil W Gibson

“…Treatment of cancer cell lines with CLN-619 induced antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis. …”
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An Antibody Screen of a Plasmodium vivax Antigen Library Identifies Novel Merozoite Proteins Associated with Clinical Protection. by Camila T França, Jessica B Hostetler, Sumana Sharma, Michael T White, Enmoore Lin, Benson Kiniboro, Andreea Waltmann, Andrew W Darcy, Connie S N Li Wai Suen, Peter Siba, Christopher L King, Julian C Rayner, Rick M Fairhurst, Ivo Mueller

“…<h4>Background</h4>Elimination of Plasmodium vivax malaria would be greatly facilitated by the development of an effective vaccine. A comprehensive and systematic characterization of antibodies to P. vivax antigens in exposed populations is useful in guiding rational vaccine design.…”
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Engineering FcRn binding kinetics dramatically extends antibody serum half-life and enhances therapeutic potential by Sanghwan Ko, Migyeong Jo, Munsu Kyung, Wonju Lee, Woo Hyung Ko, Jung-Hyun Na, Youn Seo Chun, Byoung Joon Ko, Sang Taek Jung

“…To address this, we developed an Fc variant that precisely modulates pH-dependent FcRn binding kinetics, accelerates FcRn association at acidic pH, and promotes rapid dissociation at neutral pH, thereby enhancing FcRn-driven intracellular transport, outcompeting endogenous IgG, and achieving unprecedented improvement in the serum half-life of therapeutic antibodies. Results Using comprehensive site-directed saturation mutagenesis coupled with functional screening, we generated a diverse panel of Fc variants and identified two with distinct FcRn binding kinetics: YML (L309Y/Q311M/M428L), which exhibited superior FcRn association at acidic pH and accelerated dissociation at neutral pH, and EML (L309E/Q311M/M428L), which displayed attenuated binding kinetics. …”
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Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein by Jian He, Mingzhan Luo, Mengyang Zhang, Xiaomin Hu, Yangkun Liu, Lunguang Yao

“…Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. …”
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Neutralizing monoclonal antibodies protect against human adenovirus type 55 infection in transgenic mice and tree shrews by Xinglong Liu, Zhengfeng Li, Xiao Li, Xiaoyan Zhang, Yali Zheng, Wan Su, Ying Feng, Yutong Liu, Weixuan Wu, Xikui Sun, Nana Wang, Xianmiao Ye, Zhichao Zhou, Wenkuan Liu, Jun He, Wei Wang, Linbing Qu, Rong Zhou, Ling Chen, Liqiang Feng

“…Re-emerging human adenovirus type 55 (HAdV55) has become a significant threat to public health due to its widespread circulation and the association with severe pneumonia, but an effective anti-HAdV55 agent remains unavailable. Herein, we report the generation of macaque-derived, human-like monoclonal antibodies (mAbs) protecting against HAdV55 infection with high potency. …”
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Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies by Xinyi Zhang, Xinyi Zhang, Zhongge Zhu, Peijie Zhai, Peijie Zhai, Peng Lv, You Yang, Chuanhang Cheng, Busen Wang, Ting Fang, Guanying Zhang, Xiangyang Chi, Jianmin Li, Wei Chen, Yunzhu Dong

“…Subsequently, we predicted key functional residues through structural modeling of antigen-antibody complexes and validated their roles via mutagenesis studies. …”
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Engineering of CD8+ T cells with an HIV-specific synthetic notch receptor to secrete broadly therapeutic antibodies for combining antiviral humoral and cellular immune responses by Lina Meng, Haichi Zhao, Shangkun Chang, Weiting Li, Yinghui Tian, Ruihong Wang, Libian Wang, Tiejun Gu, Jiaxin Wu, Bin Yu, Chu Wang, Xianghui Yu

“…Our findings demonstrate that the engineered CD4-17b SNR enables controllable production of functional anti-HIV-1 broadly neutralizing antibody and bispecific T cell-engaging protein upon recognition of the viral particle and cell surface antigens by the bifunctional synNotch-T cells. …”
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The Commercial Antibodies Widely Used to Measure H3 K56 Acetylation Are Non-Specific in Human and Drosophila Cells. by Sangita Pal, Hillary Graves, Ryosuke Ohsawa, Ting-Hsiang Huang, Pingping Wang, Laura Harmacek, Jessica Tyler

“…Much of our understanding of the function of histone post-translational modifications in metazoans is inferred from their genomic localization and / or extrapolated from yeast studies. …”
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A myostatin inhibitory antibody combined with insulin, partially rescues the musculoskeletal phenotype of female insulin-deficient diabetic mice by R. Clay Bunn, Reuben Adatorwovor, Philip D. Ray, Alexander R. Keeble, Christopher S. Fry, Sasidhar Uppuganti, Jeffry S. Nyman, John L. Fowlkes, Evangelia Kalaitzoglou

“…In vivo contractile muscle function testing showed that insulin increased muscle torque in diabetic mice, however there was no effect of the MyoAb. …”
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A Semi-Mechanistic Mathematical Model of Immune Tolerance Induction to Support Preclinical Studies of Human Monoclonal Antibodies in Rats by Paridhi Gupta, Josiah T. Ryman, Vibha Jawa, Bernd Meibohm

“…<b>Background/Objectives:</b> The administration of human monoclonal antibodies (mAb) in preclinical pharmacokinetics and toxicology studies often triggers an immune response, leading to the formation of anti-drug antibodies (ADA). …”
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Anti-Interleukin-16-Neutralizing Antibody Attenuates Cardiac Inflammation and Protects against Cardiac Injury in Doxorubicin-Treated Mice by Jianwei Zhang, Zicong Yang, Zhishan Liang, Mengjie Wang, Changxing Hu, Chao Chang, Lei Shi, Qingwei Ji, Ling Liu

“…Interleukin-16 (IL-16) is an important inflammatory regulator and has been shown to have a powerful effect on the regulation of the inflammatory response. …”
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