Development of a refined experimental mouse model of myasthenia gravis with anti-acetylcholine receptor antibodies by Axel You, Léa S. Lippens, Odessa-Maud Fayet, Solène Maillard, Laureline Betemps, Antony Grondin, Jean-Thomas Vilquin, Nadine Dragin, Rozen Le Panse

“…Clinical symptoms were scored, antibody subtypes against T-AChR and mouse AChR were measured, and NMJ morphology and functionality were evaluated. …”
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Development of blood-brain barrier-penetrating antibodies for neutralizing tick-borne encephalitis virus in the brain by Mizuki Fukuta, Sayo Fukano, Naoya Maekawa, Shintaro Kobayashi, Shunsuke Okamoto, Minato Hirano, Junko Nio-Kobayashi, Hiroaki Kariwa, Shigeru Kawakami, Satoru Konnai, Kentaro Yoshii

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New approach to generating of human monoclonal antibodies specific to the proteolytic domain of botulinum neurotoxin A by Marina Vladimirovna Silkina, Alena Sergeevna Kartseva, Alena Konstantinovna Riabko, Mariia Aleksandrovna Makarova, Metkhun Madibronovich Rogozin, Yana Olegovna Romanenko, Igor Georgievich Shemyakin, Ivan Alekseevich Dyatlov, Victoria Valerievna Firstova

“…Immunotherapy with neutralizing monoclonal antibodies (MAbs) is considered to be the most effective immediate response to BoNT exposure. …”
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Leveraging T cell co-stimulation for enhanced therapeutic efficacy of trispecific antibodies targeting prostate cancer by Jiaqi Zhao, Xinyu Gu, Yanping Sun, Linling Zhou, Jie Bi, Liqiang Pan

“…Furthermore, T cells treated with co-stimulatory trispecific antibodies demonstrated enhanced metabolic activity characterized by increased oxidative phosphorylation and elevated glycolysis.Conclusions Collectively, incorporating co-stimulatory receptor targeting domains represents a potentially effective strategy to unlock the full therapeutic potential of T-cell-engaging antibodies for the treatment of solid tumors.…”
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Circulating Endothelial Progenitor Cells in Patients with Established Cardiovascular Disease Treated with PCSK9 Monoclonal Antibodies by Chen Gurevitz, Osnat Itzhaki Ben Zadok, Dorit Leshem-Lev, Lital Hodeda, Aviad Rotholz, Ran Kornowski, Alon Eisen

“…Objectives: We examined the effect of proprotein convertase subtilisin kexin type 9 monoclonal antibodies (PCSK9 mAb) on cEPCs in adults with hypercholesterolemia and cardiovascular disease, aiming to establish a pleotropic class effect. …”
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γ-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. by Corina Wilding, Katharina Bell, Sabine Beck, Sebastian Funke, Norbert Pfeiffer, Franz H Grus

“…These findings let us assume a novel physiological function of γ-synuclein antibodies and give insights in the role of autoantibodies in glaucoma. …”
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Contribution of Fc-dependent cell-mediated activity of a vestigial esterase-targeting antibody against H5N6 virus infection by Zhiqiang Zheng, Su Hui Catherine Teo, Suganya Cheyyatraivendran Arularasu, Zhehao Liu, Nur Khairiah Mohd-Ismail, Chee Keng Mok, Chee Bing Ong, Justin Jang-hann Chu, Yee-Joo Tan

“…Due to the continuous evolution of this virus subtype and its ability to transmit to humans, there is an urgent need to develop effective antiviral therapeutics. In this study, a murine monoclonal antibody 9F4 was shown to display broad binding affinity against H5Nx viruses. …”
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Potency-optimized CD28-activating bispecific antibody for the targeted treatment of Nectin-4 positive cancers by Manpreet Kaur, Starlynn C Clarke, Katherine E Harris, Nathan D Trinklein, Elaine C Chen, Katja Rüger, Udaya S Rangaswamy, Laura M Davison, Sebastian Moreno Arteaga, Imani Smith, Ruth Chu, Soumili Chattopadhyay, Mathias Rickert, Shelley Force Aldred

“…Background T-cell costimulation is crucial for an effective and sustained antitumor immune response, and inadequate expression of costimulatory ligands within tumors can impair T-cell function. …”
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Experience of using polymethyl methacrylate membranes for anti-HLA antibodies’ elimination by hemodialysis in renal transplant recipients by E. I. Pervakova, V. V. Vasilets, O. N. Rzhevskaya, N. V. Borovkova, A. V. Pinchuk

“…The use of polymethyl methacrylate membrane-based dialyzers in the renal allograft recipients having a high level of preexisting anti-HLA antibodies seems promising for the period of oligoanuria until the renal allograft function is restored, since both hemodialysis and the sorption of pre-existing (and also newly synthesized) antibodies take place simultaneously.…”
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Development of monoclonal antibodies against P. gingivalis Mfa1 and their protective capacity in an experimental periodontitis model by Mingya Cao, Siyu Wang, Shengke Zhou, Min Yan, Yu Zou, Yuan Cui, Xinyu Lou, Yichang Gao, Ying Chen, Zijing Han, Yi Qian, Jingying Chen, Xia Li

“…Studies have shown that two types of fimbriae of Porphyromonas gingivalis, FimA and Mfa1, are important for colonization and infection through their binding to host tissues and other bacteria. While anti-FimA antibodies have been shown to improve periodontitis, the effect of anti-Mfa1 antibodies on P. gingivalis infection and periodontitis was previously unknown. …”
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Preclinical Characterization of Efficacy and Pharmacodynamic Properties of Finotonlimab, a Humanized Anti-PD-1 Monoclonal Antibody by Yunqi Yao, Xiaoning Yang, Jing Li, Erhong Guo, Huiyu Wang, Chunyun Sun, Zhangyong Hong, Xiao Zhang, Jilei Jia, Rui Wang, Juan Ma, Yaqi Dai, Mingjing Deng, Chulin Yu, Lingling Sun, Liangzhi Xie

“…<b>Results</b>: The results demonstrated that finotonlimab is effective in stimulating human T cell function in vitro and exhibits marked antitumor efficacy in vivo using both PD-1-humanized and PBMC-reconstructed mouse models. …”
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Preclinical characterization of the novel anti-SIRPα antibody BR105 that targets the myeloid immune checkpoint by Juan Chen, Gang Chen, Na Li, Yao Chen, Lei Nie, Zhen-Hua Wu, Xiao-Feng Mei, Xiao-Ze Wang, Ting-Ting Guo, Mei-Zhu Jiang, Ji-Teng Wang, Hai-Bin Wang

“…BR105 has a favorable safety profile and shows no adverse effects on T cell functionality. These data support further clinical development of BR105, especially as a therapeutic agent to enhance efficacy when used in combination with tumor-targeting antibodies or antibodies that target other immune checkpoints.…”
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An ANGPTL4 inhibitory antibody safely improves lipid profiles in non-human primatesResearch in context by Beryl B. Cummings, Page R. Bouchard, Mark N. Milton, Peter F. Moesta, Vyas Ramanan, John W. Trauger, Eleftheria Maratos-Flier, Andrei Voznesensky, Igor Splawski, Amitabh V. Nimonkar, Keith DiPetrillo, Daniel LaSala, Meihui Pan, Meghan M. Flaherty, Francois Huet, Sukhdeep K. Sahambi, Jijun Dong, Deborah Knee, Regis Cebe, Thomas Huber, Joshua Lehrer-Graiwer, Rebecca A. Juliano, Ethan J. Weiss

“…Human ANGPTL4 loss-of-function (LoF) is associated with reduced plasma triglyceride (TG), remnant cholesterol (RC), and apolipoprotein B (ApoB) levels, and lower risk of type 2 diabetes and ASCVD, without observable safety concerns. …”
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Integrating Biochemical and Computational Approaches Reveal Structural Insights in Trastuzumab scFv-Fc Antibody Engineering by Olga Bednova, Jessica Pougoue Ketchemen, Hazem Mslati, Mark Barok, Heikki Joensuu, Natalie Zeytuni, Francesco Gentile, Leon Sanche, Humphrey Fonge, Jeffrey Victor Leyton

“…The results showed that the scFv subunits exhibited stochastic interplays that lead to diverse shapes and were associated with functional performance. This new understanding of scFv-Fc antibodies and their structural and functional nuances provides important details to further guide the design of more effective and less toxic therapeutics.…”
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Safety and Efficacy of Influenza Vaccination in Kidney Graft Recipients in Late Period After Kidney Transplantation by Anna Zawiasa-Bryszewska, Maja Nowicka, Monika Górska, Piotr Edyko, Krzysztof Edyko, Damian Tworek, Adam Antczak, Jacek Burzyński, Ilona Kurnatowska

“…Subjective symptoms and side effects were recorded in paper journals. Antibody levels were assessed with ELISA prior to and 3 months following vaccination. …”
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Robust production of monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-Cλ interface by John D. Bagert, Vaheh Oganesyan, Chi-I Chiang, Mike Iannotti, Jia Lin, Chunning Yang, Sterling Payne, Will McMahon, Samuel Edwards, Andrew Dippel, Mark Hutchinson, Fengying Huang, Vineela Aleti, Chendi Niu, Chen Qian, Jessica Denham, Sofia Ferreira, Pallab Pradhan, Mark Penney, Chunlei Wang, Wenhai Liu, Even Walseng, Yariv Mazor

“…Bispecific antibodies represent an increasingly large fraction of biologics in therapeutic development due to their expanded scope in functional capabilities. …”
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CD47 antibody-armed oncolytic adenovirus promotes chimeric antigen receptor macrophage phagocytosis and antitumor immunity by Zhongbing Qi, Shichuan Hu, Jing Zhao, Xianglin Xu, Anliang Huang, Yu Qin, Yao Zhang, Qingzhe Yang, Jianchuan Hu, Chao Su, Ping Cheng

“…Macrophages, being a potentially effective therapeutic target, have led to the development of several therapeutic strategies due to their unique phagocytic function. …”
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Mitochondrial metabolic reprogramming of macrophages and T cells enhances CD47 antibody-engineered oncolytic virus antitumor immunity by Ping Cheng, Shichuan Hu, Zhongbing Qi, Xianglin Xu, Xiangyu Long, Anliang Huang, Jiyan Liu

“…Background Although immunotherapy can reinvigorate immune cells to clear tumors, the response rates are poor in some patients. Here, CD47 antibody-engineered oncolytic viruses (oAd-αCD47) were employed to lyse tumors and activate immunity. …”
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Siglec-15 antibody–GM-CSF chimera suppresses tumor progression via reprogramming tumor-associated macrophages by Jun Li, Hongyan Li, Zemeng Ma, Xiaoyao Hao, Wenwen Dai, Jinyu Liu, Mingjiu Chen, Shuang Qu, Quanli Zhang, Jiajing Luo, Shouyong Gu, Dihan Zhu, Ke Zen

“…Macrophage reprogramming and antigen presentation were evaluated using bone marrow-derived macrophages and human peripheral blood mononuclear cell-derived macrophages treated with anti-S15×GM CSF, followed by flow cytometry and immunofluorescence assays.Results Here we report that anti-S15×GM CSF displays superior function to suppress the progression of Siglec-15-overexpressing MC38 colon cancer engrafted in mice compared to anti-Siglec-15 antibody or GM-CSF alone. …”
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Vaccination with conserved regions of erythrocyte-binding antigens induces neutralizing antibodies against multiple strains of Plasmodium falciparum. by Julie Healer, Jennifer K Thompson, David T Riglar, Danny W Wilson, Yu-H C Chiu, Kazutoyo Miura, Lin Chen, Anthony N Hodder, Carole A Long, Diana S Hansen, Jake Baum, Alan F Cowman

“…<h4>Results</h4>This has shown that the conserved Region III-V, of the P. falciparum erythrocyte-binding antigen (EBA)-175 was able to induce antibodies that potently inhibit merozoite invasion across diverse parasite strains, including those reliant on invasion pathways independent of EBA-175 function. …”
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