A rapid and portable dual-mode RAA-CRISPR/Cas12a system for one-pot detection of spring viraemia of carp virus (SVCV) by Tian Hu, Zewei Hou, Yichen Zhao, Ye Zhang, Yufeng Si, Liqun Lu, Hao Wang

“…Sensitivity analysis revealed detection limits of 102 copies/μL for the fluorescence assay and 100 copies/μL for the LFD assay, representing a 10–1000-fold improvement over traditional nested PCR (103 copies/μL). …”
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Isolation of a SARS-CoV-2 strain from pediatric patients in South Korea: biologic and genetic characterization by Hee Chun Chung, Sung Jae Kim, Su Jin Hwang, Su Jin Hwang, Sung Hoon Park, Sung Hoon Park, Kyoung Min Park, Hyeon Woo Chung, Hyeon Woo Chung, Si Hwan Ko, Dong il Park, Jun-Yeop Shim, Van Giap Nguyen, Jae Myun Lee, Jae Myun Lee

“…Cytopathic effects appeared within 48 h post-infection, marked by aggregates of dead cells. Genomic analysis classified N15 within lineage 19B, showing 99.9% similarity to the Wuhan-Hu-1 strain. …”
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The influence of glutathione metabolism on alkaline adaptation of Amur ide (Leuciscus waleckii) and potential role of gut microbiota by Zou Yuting, Li Chenghao, Huang Jing, Yang Haochen, Luo Liang, Liew Honjung, Chang Yumei

“…Under high stress conditions (50 mmol/L), JY further synergistically upregulated gpx to activated the glutathione peroxidase (GPx) pathway to eliminate excess ROS. …”
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Bifunctional catalysts derived from mineral ores for cost-effective and robust CO2 capture and conversion by Wenqi Fan, Qian Wu, Liang Huang, Xinglei Zhao, Shipeng Ding, Qiang Wang, Ming Xue, Xingchun Li

“…Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS) analysis revealed both the redox mechanism and formate reaction pathway existed in CO2 hydrogenation to CO. …”
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Results of a Multicenter Study on the Efficacy and Safety of Inosine Glycyl-Cysteinyl-Glutamate Disodium in the Treatment of Acute Ethanol Poisoning by I. A. Shikalova, A. N. Lodyagin, V. G. Antonov, A. Y. Simonova, N. B. Loginova, E. V. Baskakov, L. G. Zaitseva, V. V. Shilov, S. P. Katasonov

“…The hepatoprotective effect of the drug was revealed, it was manifested by a decrease in ALT (p<0.001), AST (p<0.001) and direct bilirubin (p<0.03); the effect of the drug on metabolic processes — a decrease in lactate (p<0.02), an increase in BE-ECF (p<0.01), glucose (p<0.01) 3 hours after drug administration, an increase in potassium after 24 hours (p<0.03). The analysis of safety data did not reveal statistically significant differences between the treatment groups, no serious adverse events were recorded.Conclusion The study demonstrated the efficacy and safety of inosine glycyl-cysteinyl-glutamate disodium (Molixan®) in the treatment of severe ethanol poisoning in a single dose of 3.0 mg/kg administered intravenously…”
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Deciphering the anticoccidial mechanism of Qinghao Changshan formula through network pharmacology and molecular docking by Dong Tian, Hui Fu, Hongxia Tao, Miaolan Li, Qinghua Zhang, Weidong Deng

“…Eight core targets–Amyloid Beta Precursor Protein, interleukin 6, TNF Receptor Associated Factor 1, Platelet Derived Growth Factor Receptor Beta, EGFR, ESR1, Erb-B2 Receptor Tyrosine Kinase 2, and PGR–were identified through PPI network analysis. GO and KEGG enrichment revealed key pathways including focal adhesion, calcium signaling, mitogen-activated protein kinase, ErbB signaling pathway, forkhead box O, and gap junction pathways. …”
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Tracing priming effects in palsa peat carbon dynamics using a stable isotope-assisted metabolomics approach by Christian Ayala-Ortiz, Moira Hough, Elizabeth K. Eder, David W. Hoyt, Rosalie K. Chu, Jason Toyoda, Steven J. Blazewicz, Patrick M. Crill, Ruth Varner, Scott R. Saleska, Virginia I. Rich, Virginia I. Rich, Malak M. Tfaily, Malak M. Tfaily

“…The SIAM approach detected subtle molecular changes indicating minimal and transient priming activity that was undetectable through conventional gas measurements alone. …”
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Screening and identification of HLA-A2-restricted neoepitopes for immunotherapy in endocrine therapy-resistant breast cancer by Mingshuang Wang, Liwei Pang, Yingjie Sun, Jingjing Han, Jiani Fan, Wenhui Shen, Xiaonan Hu, Bingqian Yang, Haoming Ning, Yanan Kong, Duo Li, Wenshan Zhao, Ranran Shi, Ling Ran, Yuanming Qi, Yahong Wu

“…The induced CTLs exhibited specific recognition and cytotoxic activity against both T2A2 cells loaded with mutant neoepitopes and HLA-A*0201-positive breast cancer cells transfected with minigene encoding mutant neoepitopes. …”
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Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways by Sofie Rennen, Victor Bosteels, Clint De Nolf, Sandra Maréchal, Jessica Vetters, Karo Van Lil, Greta Webb, Janne Swinnen, Evelien Van De Velde, Farzaneh Fayazpour, Ria Roelandt, Niels Vandamme, Kevin Verstaen, Stefaan C. De Smedt, Karine Breckpot, Stijn Vanhee, Philippe Gevaert, Eric Hoste, Bruno G. De Geest, Rein Verbeke, Ine Lentacker, Sophie Janssens

“…This reveals that LNPs are not decoded as “dangerous” by DCs, and that the cargo is essential to provide adjuvant activity, which is highly relevant for the targeted design of LNP-based therapies.…”
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Development of a Method and a Scheme for the Use of a Live Recombinant Vaccine "GamLPV". Safety and Tolerability of Double Intranasal Vaccination of Healthy Adult Volunteers by G. I. Karataev, A. Yu. Medkova, E. G. Semin, L. N. Sinyashina, R. A. Sioundioukova, S. V. Kulikov, N. A. Snegireva, I. N. Chernishova, M. V. Gavrilova, K. K. Bushkova, A. A. Lidzhieva, L. V. Kolobukhina, I. S. Kruzhkova, L. N. Merkulova, M. G. Rusanova, M. V. Bazarova, N. A. Antipyat, S. V. Smetanina, E. A. Smolyarchuk, I. N. Dyakov

“…Both proposed methods of double administration can be used to plan a multicenter study to research the immunogenicity and protective activity of GamLPV.…”
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MODERN PECULIARITIES OF THE MEDICAL EXPOSURE LEVELS FORMING OF THE TATARSTAN REPUBLIC POPULATION DURING X-RAY PROCEDURES IMPLEMENTATION by S. A. Ryzhkin, S. I. Ivanov, M. A. Patjashina, R. K. Ismagilov, N. A. Akopova, S. V. Loginova, D. R. Nasybullin

“…The number of special investigations has increased in 2.1 times, but the relative value remained at average level of 0.4% on general background of the increasing of X-ray activity in the region. This fact influenced the change in the radiation-hygienic indexes of medical radiation exposure of the population of RT. …”
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Characterization of the virulence shaping and adaptability in the methicillin-resistant Staphylococcus aureus ST9 lineage by Yiyi Chen, Feiteng Zhu, Yueqin Hong, Yeqiong Liu, Haiping Wang, Shengnan Jiang, Mengzhen Chen, Shujuan Ji, Zhengan Wang, Yunsong Yu, Yan Chen, Lu Sun

“…These agr-deficient strains exhibited reduced hemolytic activity, enhanced biofilm formation, and attenuated virulence in a Galleria mellonella infection model. …”
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Mechanisms of Cefiderocol Resistance in Carbapenemase-Producing Enterobacterales: Insights from Comparative Genomics by Alexander Tristancho-Baró, Ana Isabel López-Calleja, Ana Milagro, Mónica Ariza, Víctor Viñeta, Blanca Fortuño, Concepción López, Miriam Latorre-Millán, Laura Clusa, David Badenas-Alzugaray, Rosa Martínez, Carmen Torres, Antonio Rezusta

“…<b>Background/Objectives</b>: Cefiderocol is a novel siderophore cephalosporin with potent in vitro activity against a broad spectrum of Gram-negative bacteria, including carbapenemase-producing Enterobacterales (CPE). …”
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Integrated genome mining and molecular networking uncover the biosynthetic potential of a novel mangrove-derived strain Streptomyces sp. B1866 by Liyuan Jiang, Wenjin Hu, Jinling Qin, Baorui Teng, Siwei Chen, Dan Zhang, Hui Lei, XinLi Pan

“…The structure of 1 was deduced from the analysis of 1D/2D NMR, HRESIMS data, and electronic circular dichroism (ECD) comparisons. …”
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Role of functional mapping on Gallium-68 perfusion positron emission tomography and computed tomographic imaging (PET/CT) to assess the risk of long-term radiation-induced lung tox... by François Lucia, David Bourhis, Frédérique Blanc-Béguin, Gaëlle Goasduff, Mohamed Hamya, Simon Hennebicq, Maëlle Mauguen, Romain Floch, Margaux Geier, Ulrike Schick, Maëlys Consigny, Olivier Pradier, Grégoire Le Gal, Pierre-Yves Salaun, Vincent Bourbonne, Pierre-Yves Le Roux

“…The primary endpoint of this analysis was grade ≥2 long-term RILT at 12 months as assessed with NCI CTCAE v.5. …”
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EXPLORING THE CORRELATION BETWEEN GAIT SPEED AND BALANCE IN LIMB PROSTHESIS USERS: A PILOT STUDY by Abdullah Alhuzaymi, Goeran Fiedler

“…Possible confounding variables include the activity level and the respectively prescribed prosthetic technology. …”
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The Costs of Digital Health Interventions to Improve Immunization Data in Low- and Middle-Income Countries: Multicountry Mixed Methods Study by Carlo Federici, Maria Verykiou, Marianna Cavazza, Willyhelmina Olomi, Piero Irakiza, Kizito Kayumba, Edith Rodriguez, Luis Enrique Castillo Mendoza, Stefano Malvolti, Claire Hugo, Nyanda Elias Ntinginya, Souleymane Camara, Issa Sabi, Nagnouma Sano, Hassan Sibomana, Jeanine Condo, Aleksandra Torbica, Claudio Jommi, Carsten Mantel, Viviana Mangiaterra

“…Primary survey data were collected to gauge the operational expenses of immunization information systems, both with and without electronic tools, using an activity-based costing approach in 275 facilities. …”
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Vitamin A deficiency triggers colonic methylation potentially impairing colonic neuron via downregulation SGK1/FOXO pathway by Bei Tong, Junyan Yan, Zhujun Sun, Ruifang Luo, Fang Lin, Riqiang Hu, Ting Yang, Yuting Wang, Jie Chen

“…A combined methylomic and transcriptomic analysis identified that methylation levels of Sgk1, a key gene associated with enteric neural development, were elevated in the VAD group, and the activity of the SGK1/FOXO signaling axis was reduced. …”
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Increased Levels of Erythropoietin in Nipple Aspirate Fluid and in Ductal Cells from Breast Cancer Patients by Ferdinando Mannello, Laura Fabbri, Eleonora Ciandrini, Gaetana A. Tonti

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HEALTH TECHNOLOGIES FOR INCREASING ADAPTIVE CAPABILITIESOF PREGNANT WOMEN by T. S. Krivonogova, O. M. Gerget, A. A. Ber, Ya. S. Pekker

“…Rotov was used, namely, the consideration of information measure as a measure of biological object preference behavior. Analysis of the results with information criterion of curve types using cluster analysis allowed to identify 4 standard types of adaptation strategies for pregnant women: adaptive, compensatory, compensatory-adaptive and maladaptive compensatory type of response. …”
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