The TgAMPK-TgPFKII axis essentially regulates protein lactylation in the zoonotic parasite Toxoplasma gondii by Chenghuan Li, Yang Zhao, Qilong Li, Ran Chen, Ying Feng, Xiaoyu Sang, Xiangrui Li, Bang Shen, Ning Jiang, Qijun Chen

“…By elucidating the cellular localization and functional importance of TgPFKII, as well as its regulation through AMPK-specific inhibitors, we provide comprehensive insights into the metabolic and signaling networks that underpin T. gondii pathogenicity. …”
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CPLX1 is a novel prognostic biomarker in CRC correlating with immunotherapy resistance and ferroptosis by Canyu Liu, Qiujun Liu, Yuanhao Lv, Tingmin Chang, Shiyi Song, Yuang Ding, Jiateng Zhong, Jiateng Zhong, Jiateng Zhong, Jiateng Zhong, Jiateng Zhong, Yanxuan Liu

“…Additionally, mutational analyses through cBioPortal and COSMIC datasets were employed to identify CPLX1 mutations in COAD. Co-expression and functional enrichment analyses, alongside Gene Set Enrichment Analysis (GSEA), were also conducted to delineate pathways impacted by CPLX1.ResultsOur findings indicate that high expression levels of CPLX1 are significantly correlated with poor prognostic outcomes in CRC patients. …”
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Intravenous Nanoemulsions Loaded with Phospholipid Complex of a Novel Pyrazoloquinolinone Ligand for Enhanced Brain Delivery by Tijana Stanković, Tanja Ilić, Branka Divović Matović, Milos Petkovic, Vladimir Dobričić, Ivan Jančić, Biljana Bufan, Kristina Jezdić, Jelena Đoković, Ivana Pantelić, Danijela Randjelović, Dishary Sharmin, James M. Cook, Miroslav M. Savić, Snežana Savić

“…There were subtle differences in the pharmacokinetic parameters in favor of a dual surface-functionalized nanoemulson containing the glucose transporter-1-targeting ligand (mannose). …”
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Predictive Role of Complete Blood Count-Derived Inflammation Indices and Optical Coherence Tomography Biomarkers for Early Response to Intravitreal Anti-VEGF in Diabetic Macular Ed... by Ece Ergin, Ana Maria Dascalu, Daniela Stana, Laura Carina Tribus, Andreea Letitia Arsene, Marina Ionela Nedea, Dragos Serban, Claudiu Eduard Nistor, Corneliu Tudor, Dan Dumitrescu, Paul Lorin Stoica, Bogdan Mihai Cristea

“…<b>Conclusions:</b> CBC-derived inflammation indices and OCT biomarkers have prognostic value in predicting early response to anti-VEGF therapy in DME in terms of functional and anatomical outcomes. These findings could help identify poor responders and guide personalized treatment strategies.…”
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Mitigating heat stress in Garut lambs: Synergistic effects of Lemuru fish oil, vitamin E, and selenium on antioxidant defense, hematology, and physiological responses by Aeni Nurlatifah, Dewi Apri Astuti, Herdis Herdis, Iis Arifiantini, Fitra Aji Pamungkas, Santoso Santoso, Didid Diapari, Pradita Iustitia Sitaresmi, Enny Tantini Setiatin, Athar Manabi Diansyah

“…Background and Aim: Tropical climates expose livestock to prolonged heat stress, leading to compromised health, growth, and productivity. …”
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The short-chain fatty acid receptors Gpr41/43 regulate bone mass by promoting adipogenic differentiation of mesenchymal stem cells by Friederike Behler-Janbeck, Anke Baranowsky, Timur A. Yorgan, Michelle Y. Jaeckstein, Anna Worthmann, Marceline M. Fuh, Karthikeyan Gunasekaran, Gisa Tiegs, Michael Amling, Thorsten Schinke, Joerg Heeren

“…SCFAs exert various metabolic functions, which in part can be explained by activation of G protein-coupled receptors (Gpr) 41 and 43. …”
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Identification of prognostic biomarkers related to epithelial-mesenchymal transition and anoikis in hepatocellular carcinoma using transcriptomics and single-cell sequencing by Maobing Wang, Lu Cheng, Lu Cheng, Lu Cheng, Lu Cheng, Kuo Qi, Kuo Qi, Kuo Qi, Kuo Qi, Haiping Wang, Haiping Wang, Haiping Wang, Haiping Wang, Xun Li, Xun Li, Xun Li, Xun Li

“…Subsequently, the potential functions of biomarkers were explored through methods such as enrichment analysis and immune microenvironment analysis. …”
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YWHAG promotes bladder cancer metastasis by regulating TMOD3 to activate ERK1/2 and JNK phosphorylation in the MAPK pathway by Tai Tian, Shiming He, Han Hao, Bao Guan, Yanqing Gong, Jian Fan, Zhenpeng Zhu, Wenzhi Gao, Yucai Wu, Ninghan Feng, Aixiang Wang, Yuexian Guo, Xuesong Li

“…Abstract Objective This study aims to investigate the molecular mechanisms by which YWHAG (Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma) promotes metastasis in bladder cancer. …”
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Effects of High-Concentrate Diets on Growth Performance, Serum Biochemical Indexes, and Rumen Microbiota in House-Fed Yaks by Ben Zhang, Xingdong Wang, Ziqiang Ding, Yandong Kang, Shaoke Guo, Mengli Cao, Liyan Hu, Lin Xiong, Jie Pei, Xian Guo

“…The aim of this study is to determine the effects of a high-concentrate diet on growth performance, serum biochemical indexes, and rumen microbiota in house-fed yaks. …”
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Design and methods of an adaptive trial to test comparative effectiveness of readmission reduction approaches following infection and sepsis hospitalizations (ACCOMPLISH) by Kristin Mayes, Victor B. Talisa, Adelina Malito, Florian B. Mayr, Kelly Williams, Kalpana Char, Richard Wadas, Elizabeth Lorenzi, Kert Viele, Rana Awdish, Derek C. Angus, Chung-Chou Ho Chang, Sachin Yende

“…Secondary endpoints, such as functional status and health-related quality of life, will be measured at baseline and 90 days. …”
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Chloroquine induces eryptosis in P. falciparum-infected red blood cells and the release of extracellular vesicles with a unique protein profile by Claudia Carrera-Bravo, Claudia Carrera-Bravo, Tianchi Zhou, Tianchi Zhou, Trang Chu, Jing Wen Hang, Harshvardhan Modh, Chenyuan Huang, Chenyuan Huang, Sitong Zhang, Sitong Zhang, Haining Hao, Haining Hao, Haining Hao, María José Cabada-García, Benoit Malleret, Benoit Malleret, Matthias G. Wacker, Jiong-Wei Wang, Jiong-Wei Wang, Jiong-Wei Wang, Jiong-Wei Wang, Laurent Rénia, Kevin S. W. Tan

“…Notably, the outward budding and blebbing of the iRBC plasma membrane led to the formation of EVs, which are complex structures with unique functional properties. Proteomic characterization of EVs from CQ-treated iRBCs revealed a high enrichment of proteasome and ribosome protein clusters. …”
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Direct-fed microbials optimize ruminal fermentation, microbial ecosystem and milk quality to enhance the lactation performance of Sanhe dairy cows by Aoyu Jiang, Zixin Liu, Ziyan Yang, Shizhe Zhang, Jian Wu, Chuanshe Zhou, Zhiliang Tan

“…In predictive analysis of microbial functional genes, the treatment group was enriched with more genes encoding hemicellulose-degrading enzymes such as GH10, GH30, and GH67, while the relative abundance of genes encoding enzymes that metabolize ammonia was increased. …”
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A genome-wide association study identifies new loci associated with response to SARS-CoV-2 mRNA-1273 vaccine in a cohort of healthy healthcare workers by Antonio José Cabrera-Serrano, Antonio José Cabrera-Serrano, Lucía Ruiz-Durán, Lucía Ruiz-Durán, Juan Francisco Gutiérrez-Bautista, Juan Francisco Gutiérrez-Bautista, Juan Francisco Gutiérrez-Bautista, Juan Francisco Gutiérrez-Bautista, María Carretero-Fernández, María Carretero-Fernández, Rob ter Horst, Rob ter Horst, Rob ter Horst, Yang Li, Yang Li, Fernando Jesús Reyes-Zurita, Francisco José García-Verdejo, Francisco José García-Verdejo, Mihai G. Netea, Mihai G. Netea, Pedro Sánchez-Rovira, Pedro Sánchez-Rovira, Miguel Ángel López-Nevot, Miguel Ángel López-Nevot, Miguel Ángel López-Nevot, Antonio Sampedro, Antonio Sampedro, Juan Sainz, Juan Sainz, Juan Sainz, Juan Sainz

“…Functional results showed that some of the novel risk alleles influence the absolute counts of specific B cell subsets (p=2.57×10-5-8.82×10-3), which are involved in immune signaling pathways and metabolic processes. …”
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EURAD state-of-the-art report: thermo-hydro-mechanical behaviour of clay buffers at high temperatures by María Victoria Villar, Katerina Cernochova, Jaime Cuevas, Antonio Gens, Natalia Gimeno, Caroline Graham, Jon Harrington, Vlastislav Kašpar, Stephan Kaufhold, Olivier Leupin, David Mašín, Jan Najser, Markus Olin, Heini Reijonen, Šárka Šachlová, Sergey Sayenko, Daniel Svensson, Jiri Svoboda, Gianni Vettese, Janne Yliharju, Borys Zlobenko

“…Given that being able to tolerate higher temperatures would have significant advantages, the work package HITEC of the EURAD project aimed at determining the influence of temperature above 100°C on buffer properties, trying to establish if the safety functions are unacceptably impaired. …”
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Prognostic value of global plaque volume calculated from the 3D reconstruction of the coronary tree in patients without significant coronary artery disease. A multicenter study by Carlos Cortés, Julio Ruiz-Ruiz, Fernando Rivero, Ramón López-Palop, Octavio Jiménez, Alfonso Freites, Luis R. Gonçalves-Ramírez, María Rosario Ortas Nadal, Sara Blasco, Mario García-Gómez, Clara Fernández, Luca Scorpiglione, J. Alberto San Román Calvar, Ignacio J. Amat-Santos

“…However, some patients without significant angiographic coronary artery disease will experience cardiovascular events at the follow-up. This study aims to determine the prognostic value of the global plaque volume (GPV) in patients with stable coronary artery disease without functionally significant lesions at a 5-year follow-up. …”
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SARS-CoV-2 immune responses in patients with multiple myeloma and lenalidomide maintenance therapy by Ioana Martac, Sina A. Beer, Aileen Schenk, Osama Ahmad, Claus-Philipp Maier, Claus-Philipp Maier, Gülay Demirel, Beate Preuß, Reinhild Klein, Anna M. P. Stanger, Britta Besemer, Luca Hensen, Claudia Lengerke

“…Here, immunomodulatory drugs (IMiDs) like Lenalidomide (Lena) become interesting, as they may have stimulatory effects on T-cell functioning.MethodsIn this study we investigated immune responses elicited by COVID-19 vaccine or infection comparing 43 healthy volunteers (avg. 35y, 72.1% female, 81.4% previously COVID-19 infected), with 41 MM patients under Lena maintenance therapy (avg. 63.8y, 51.2% female, 61% previously COVID-19 infected). …”
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Comprehensive analysis of exosome-related gene signature in multiple myeloma prognosis and immune microenvironment evaluation by Dong Zheng, Bingxin Zhang, Quanqiang Wang, Sisi Zheng, Yibo Xia, Ziwei Zheng, Zhili Lin, Shuxia Zhu, Xinyi Zhang, Luning Cui, Hansen Ying, Tianyu Zhang, Shujuan Zhou, Zixing Chen, Enqing Lan, Yu Zhang, Xuanru Lin, Jingjing Chen, Honglan Qian, Xudong Hu, Yan Zhuang, Zuoting Xie, Xiangjing Zhou, Zhouxiang Jin, Songfu Jiang, Yongyong Ma

“…However, the connection between myeloma and exosome-related genes (ERGs) has not been explored. Therefore, we aim to establish a more accurate model to evaluate the prognosis of MM patients based on the exosome-related genes. …”
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DREB Genes from Common Bean (Phaseolus vulgaris L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity by Enéas Ricardo Konzen, Gustavo Henrique Recchia, Fernanda Cassieri, Danielle Gregorio Gomes Caldas, Jorge C. Berny Mier y Teran, Paul Gepts, Siu Mui Tsai

“…The expression patterns and nucleotide diversity of each DREB found in this study revealed fundamental characteristics for further research aimed at understanding the molecular mechanisms associated with drought, salt, and cold tolerance in common bean, which could be performed based on association mapping and functional analyses.…”
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lncRNA Ubr5 promotes BMSCs apoptosis and inhibits their proliferation and osteogenic differentiation in weightless bone loss by Dong Wang, Yuan Gao, Yingjun Tan, Na Li, Xi Li, Jiaxiang Li, Yikai Pan, Xingcheng Zhao, Ming Yan, Yongchun Wang

“…Subsequently, the molecular functions and signaling pathways enriched by DEG were comprehensively analyzed by GO and KEGG. …”
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Unique immune characteristics and differential anti-PD-1-mediated reinvigoration potential of CD8+ TILs based on BRCA1/2 mutation status in epithelial ovarian cancers by SungHoon Kim, Sang Wun Kim, Su-Hyung Park, Eui-Cheol Shin, Junsik Park, Yong Jae Lee, Jung Yun Lee, Mi-Kyoung Seo, Jung Chul Kim

“…Background We aimed to investigate the distinct immunological characteristics of the tumor immune microenvironment in epithelial ovarian cancer (EOC) according to BRCA1/2 mutations status and differential PD-1 expression levels.Methods Tumor-infiltrating lymphocytes (TILs) were collected from patients with newly diagnosed advanced-stage EOC (YUHS cohort, n=117). …”
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