A Peptide‐Derived Aggregation‐Induced Emission Nanobioprobe: Unlocking Selective Detection of Fusobacterium nucleatum and Noninvasive Screening of Colorectal Cancer by Hongyu Liu, Tengling Wu, Yunjian Yu, Youtao Xin, Hegang Lu, Shengke Zhao, Meihui Su, Lu Ga, Alideertu Dong, Mahmoud Elsabahy, Hui Gao

“…However, the current detection methods involve exorbitant costs and complex procedures, which are inconvenient for large‐scale screening. …”
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Analysis of polyclonal and monoclonal antibody to the influenza virus nucleoprotein in different oligomeric states by Mallory L. Myers, Michael T. Conlon, John R. Gallagher, De'Marcus D. Woolfork, Noah D. Khorrami, William B. Park, Regan K. Stradtman-Carvalho, Audray K. Harris

“…Furthermore, we show that the classical mouse anti-NP mAb HB65 could detect NP in some commercial influenza vaccines. MAb HB65 bound a linear epitope with nanomolar affinity. …”
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A novel fluorescent probe, triphenylamine rhodamine-3-acetic acid (mRA) for the detection of Amyloid-β aggregates in Alzheimer's disease by Raja Chinnappan, Raja Chinnappan, Mateen A. Khan, Taj Mohammad, Sarah Mohammed Allwaibh, Shanmugam Easwaramoorthi, Ahmed Yaqinuddin, Sandhanasamy Devansan, Tanveer Ahmad Mir, Tanveer Ahmad Mir, Imtaiyaz Hassan

“…Herein, an aggregation-induced fluorescence probe, triphenylamine rhodamine-3-acetic acid (mRA), was used to detect Aβ aggregates. The fluorescence results showed a strong interaction between the fluorescence probe mRA and Aβ aggregates. mRA specifically binds with high affinity to Aβ aggregates, and the limit of detection (LOD) of Aβ aggregates was 0.12 μg/mL. …”
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Detection of Calmodulin-Binding Proteins Using a 32P-Labeled GST-Calmodulin Fusion Protein and a Novel Renaturation Protocol by Roland Fischer, Yu Wei, Martin Berchtold

“…The fusion protein is easily purified from bacterial lysates by affinity chromatography using glutathione-Sepharose® 4B beads. …”
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Artificial-Intelligence Bio-Inspired Peptide for Salivary Detection of SARS-CoV-2 in Electrochemical Biosensor Integrated with Machine Learning Algorithms by Marcelo Augusto Garcia-Junior, Bruno Silva Andrade, Ana Paula Lima, Iara Pereira Soares, Ana Flávia Oliveira Notário, Sttephany Silva Bernardino, Marco Fidel Guevara-Vega, Ghabriel Honório-Silva, Rodrigo Alejandro Abarza Munoz, Ana Carolina Gomes Jardim, Mário Machado Martins, Luiz Ricardo Goulart, Thulio Marquez Cunha, Murillo Guimarães Carneiro, Robinson Sabino-Silva

“…Cyclic voltammetry (CV) using a [Fe(CN)₆]^3−/4 solution detected virus levels in saliva samples with and without SARS-CoV-2. …”
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CNF: An Automated Test Case Generation Method Based on Neuron Coverage Guidance in Key Network Layers by Yiran Gu, Yunlai Sun, Yiling Wu, Fei Yan

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An Unlabeled Electrochemical Immunosensor Uses Poly(thionine) and Graphene Quantum Dot-Modified Activated Marigold Flower Carbon for Early Prostate Cancer Detection by Suparat Cotchim, Supatinee Kongkaew, Panote Thavarungkul, Proespichaya Kanatharana, Warakorn Limbut

“…The sensor could detect concentrations as low as 0.005 ng mL⁻¹, and had a quantification limit of 0.017 ng mL⁻¹. …”
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A novel MRI contrast agent NaGdF4@PEG–CLS@MMP-13 NPs for detecting articular cartilage injury by Jian Xu, Yijun Zhang, Mao Lin, Binbin Ma, Hongpu He, Fangyi Jiang, Shukun He, Chengjie Yuan

“…Abstract Early detection of cartilage injuries is crucial due to their limited self-repair capacity and risk of joint dysfunction. …”
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Synergistic Signal Amplification via Weak Value Amplification Effect and Sandwich Structure for Highly Sensitive and Specific Real-Time Detection of CA125 by Bei Wang, Yang Xu, Han Li, Zishuo Song, Tian Guan, Yonghong He

“…The dual-recognition mechanism combines the high affinity of aptamers and the specificity of antibodies, significantly improving detection performance while utilizing antibodies for signal amplification. …”
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A Novel Chiral Molecularly Imprinted Electrochemical Sensor Based on β-CD Functionalized Graphene Quantum Dots for Enantioselective Detection of D-Carnitine by Feng Yang, Xin Qi, Yan Chen, Kai Tang, Mengyang Fang, Yanwei Song, Jiufen Liu, Lianming Zhang

“…In this study, β-cyclodextrin (β-CD) functionalized graphene quantum dots (GQDs) was employed to augment the array of chiral recognition sites, thereby enhancing the affinity of GQDs/β-CD composite for imprinting molecules and realizing heightened chiral selectivity. …”
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A simple, rapid, and low-cost approach for colloidal nanoparticle-based surface enhanced Raman Scattering detection of endosulfan pesticide at trace levels by A Falamas, I A Brezestean, N Tosa, S Boca, C Farcau

“…Surface Enhanced Raman Spectroscopy (SERS) is a highly specific, sensitive, and portable technique with great potential for on-site pesticide detection and monitoring. Endosulfan, an organochlorine pesticide known for its high toxicity, slow degradation, and bioaccumulation, has poor affinity for metallic SERS substrates. …”
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Investigation of the pathogenic potential and the possibility of cross-species transmission of H5 avian influenza viruses detected on the territory of the Russian Federation in 201... by Nikolay G. Zinyakov, Alena D. Grekhneva, Artem V. Andriyasov, Evgeniya V. Ovchinnikova, Nelli A. Guseva, Anton A. Kozlov, Zoya B. Nikonova, Pavel D. Zhestkov, Dmitry B. Andreychuk, Ilya A. Chvala

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Non-lethal detection of mtDNA from the monogenean Gyrodactylus mutabilitas which recorded for the first time from the gill mucus of Cyprinus carpio, Iraq by Rana Shalal

“…The distribution of this monogenean on the gill arches of the hosts revealed a specific right-side preference, with a notable affinity for the second gill arches. Typically, accurate diagnosis of the monogenean relies on traditional methods by their morphology and it can only be achieved by fish post-mortem dissection. …”
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In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter by Ka Lok Hong, Eamonn Maher, Ryan M. Williams, Letha J. Sooter

“…A modified sandwich ELISA assay was developed utilizing the selected ssDNA MRE as the antigen capturing element and achieved a sensitive detection of 50 nM of toxin B in human fecal preparations.…”
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Preclinical and clinical evaluation of [64Cu]Cu-PSMA-Q PET/CT for prostate cancer detection and its comparison with [18F]FDG imaging by Fei Chen, Hao Zhang, Yousheng Zhan, Xiaohong Huang, Zongxi He, Daiyuan Ma, Tielong Tang, Suping Li

“…Its binding affinity, cellular uptake, and internalization were assessed in vitro using prostate-specific membrane antigen (PSMA)-positive LNCaP C4-2B cells. …”
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In evolution’s unending race: ancestral STING sensors in Salmo salar mediate intracellular bacterial detection and programmed cell death through evolutionarily conserved pathways by Alejandro J. Yañez, Alejandro J. Yañez, Alejandro J. Yañez, Alejandro J. Yañez, Jorge F. Beltrán, Claudia A. Barrientos, Genaro Soto-Rauch, Marcelo Aguilar, Marcelo Aguilar, Adolfo Isla, Adolfo Isla, Adolfo Isla, Sandra N. Flores-Martin, Francisco T. Yañez, Yassef Yuivar, Adriana Ojeda, Felipe Almendras, Patricio Bustos, Marcos Mancilla

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Remapping the spatial distribution of neutralizing sites and their immunodominance on the capsid of different topotypes of FMDV serotype O by site-directed competitive ELISA for de... by Qiongqiong Zhao, Fengjuan Li, Shulun Huang, Xiangchuan Xing, Ying Sun, Pinghua Li, Huifang Bao, Yuanfang Fu, Pu Sun, Xingwen Bai, Hong Yuan, Xueqing Ma, Zhixun Zhao, Jing Zhang, Jian Wang, Tao Wang, Dong Li, Qiang Zhang, Ping Qian, Yimei Cao, Kun Li, Xiangmin Li, Zengjun Lu

“…This study provides comprehensive insights into the antigenic structure of FMDV serotype O and lays the groundwork for developing new methods to detect NAbs.…”
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Influence-Based Community Partition with DegreeRank Label Propagation (DRLP) Algorithm for Social Networks by Mingwu Li, Ailian Wang, Xuyang Gao, Bolin Li

“…We present a damping factor reflecting the affinity between nodes, which can be adjusted to affect the change of affinity between nodes caused by unexpected events, aiming to simulate interpersonal communication in real networks. …”
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Tailored Fluorescent Metal–Organic Frameworks Hybrid Membrane Sensor Arrays: Simultaneous and Selective Quantification of Multiple Antibiotics by Tongtong Ma, Qiao Huang, Lei Yuan, Shugang Yan, Yalin Mo, Yibin Ying, Yingchun Fu, Jinming Pan

“…Abstract Sensor array offers significant potential for rapid, high‐throughput antibiotic detection. However, cross‐reactivity‐based sensor arrays often lack accuracy, despite comprehensive data analysis; while traditional high‐affinity‐based sensors based on antibodies/aptamers frequently suffer from complicated design and poor robustness. …”
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Development and validation of an analytical procedure for the determination of residual protein A in active substances of therapeutic monoclonal antibodies by E. V. Nozdrina, D. A. Mazalev, D. R. Rogozina, S. P. Zhivoderov, I. V. Lyagoskin, R. R. Shukurov

“…An important quality-control issue for therapeutic monoclonal antibodies (mAbs) is the determination of residual protein A leaching from the carrier during the purification of mAbs by affinity chromatography. However, unrelated compounds (immunoglobulins) present in the sample can complicate the immunoenzymatic detection of protein A (matrix effect), potentially leading to false-negative test results. …”
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