Tomato yellow leaf curl virus detection based on cross-domain shared attention and enhanced BiFPN by Henghui Mo, Linjing Wei

“…Based on the YOLOv8n model, we propose the YOLOv8n-CDSA-BiFPN model for detecting TYLCV. We introduce an Adaptive Random Mix-Cut Splicing (ARMS) image augmentation technique that merges affected leaf images with healthy leaves, enhancing the training and validation datasets through noise addition, geometric transformations, color changes, and affine transformations, thus increasing the diversity of background targets. …”
Get full text

Sensitive and specific detection of the non-human sialic Acid N-glycolylneuraminic acid in human tissues and biotherapeutic products. by Sandra L Diaz, Vered Padler-Karavani, Darius Ghaderi, Nancy Hurtado-Ziola, Hai Yu, Xi Chen, Els C M Brinkman-Van der Linden, Ajit Varki, Nissi M Varki

“…Thus, there is need for the sensitive and specific detection of Neu5Gc in human tissues and biotherapeutic products. …”
Get full text

Unsupervised Binary Classifier-Based Object Detection Algorithm with Integrated Background Subtraction Suitable for Use with Aerial Imagery by Gabija Veličkaitė, Ignas Daugėla, Ivan Suzdalev

“…The proposed system, SARGAS, combines a custom convolutional neural network (CNN) classifier with MOG2 background subtraction and partial affine transformations for camera stabilization. A secondary CNN refines detections and reduces false positives. …”
Get full text

The staphylococcal collagen adhesin CNA35 effectively detects collagen and its fragments in blots after SDS-PAGE by Elena N. Pokidysheva, Jennifer Diaz Sales, Shinomi Yagi, Tomonori Ueno, Kanako Sasai, Alice Makarenko, Hans Peter Bächinger, Kazunori Mizuno, Sergei P. Boudko

“…The extracellular portion of CNA, known as CNA35, which has a molecular weight of 35 kDa, has been used for in vitro, ex vivo, and in vivo staining of various collagen types in tissues.Detecting various types of collagens necessitates the use of type- and specie-specific antibodies, which typically exhibit weak affinities for the triple helical regions of collagens. …”
Get full text

Novel, Speedy, and Eco-Friendly Carboxymethyl Cellulose-Nitrogen Doped Carbon Dots Biosensors with DFT Calculations, Molecular Docking, and Experimental Validation by Hebat-Allah S. Tohamy

Get full text

Engineering functionally-optimized aptamers against SARS-Cov-2 for blocking spike-ACE2 interaction and aptasensor detection by Xiangdong Lai, Weiwei Zhao, Lihua Jiang, Jing Li, Muhammad Faizan Munawer, Jiejuan Lai, Jesus Alejandro Martinez Juarez, Miraj Ud din, Xiaoyang Zhang, Zhongquan Song, Tao Wu, Yiyue Ge, Hui Jiang, Xiaohui Liu, Xuemei Wang

“…The progeny Seq3 exhibited a higher affinity for S1 and a better blocking effect on S1-angiotensin-converting enzyme 2 (ACE2) interaction compared to Apt2. …”
Get full text

A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein by Sonia Mukherjee, Pierre Leblanc, Mark C Poznansky, Ann E Sluder

Get full text

Clustering by Detecting Density Peaks and Assigning Points by Similarity-First Search Based on Weighted K-Nearest Neighbors Graph by Qi Diao, Yaping Dai, Qichao An, Weixing Li, Xiaoxue Feng, Feng Pan

Get full text

Moving Vehicle Detection and Tracking Based on Optical Flow Method and Immune Particle Filter under Complex Transportation Environments by Wei Sun, Min Sun, Xiaorui Zhang, Mian Li

“…Video-based moving vehicle detection and tracking is an important prerequisite for vehicle counting under complex transportation environments. …”
Get full text

A protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. by Kailash P Patra, Mayuko Saito, Vidya L Atluri, Hortensia G Rolán, Briana Young, Tobias Kerrinnes, Henk Smits, Jessica N Ricaldi, Eduardo Gotuzzo, Robert H Gilman, Renee M Tsolis, Joseph M Vinetz

“…We sought to optimize a monoclonal antibody-based assay to detect Brucella melitensis lipopolysaccharide in blood by conjugating B. melitensis LPS to keyhole limpet hemocyanin, an immunogenic protein carrier to maximize IgG affinity of monoclonal antibodies. …”
Get full text

Penicillin-Binding Proteins and Graphene/Chitosan Nanocomposite-Based Electrochemical Assay for Multiple Beta-Lactam Antibiotics Detection in Milk by Senwei Jiang, Hangzhen Lan, Daodong Pan, Lu Huang, Yichun Wu

“…PBP1a and PBP2x, which have high affinity and specificity for the six BLAs, were used as the receptors and their performance was compared. …”
Get full text

Oxidase-Like Catalytic Performance of Nano-MnO2 and Its Potential Application for Metal Ions Detection in Water by Kai Sun, Qingzhu Liu, Rui Zhu, Qi Liu, Shunyao Li, Youbin Si, Qingguo Huang

“…MnO2 nanozyme-based detecting systems achieved high accuracy (relative errors: 2.2–26.1%) and recovery (93.0–124.0%) for detection of the four metal ions, respectively. …”
Get full text

Sensitive and rapid detection of bisphenol A using signal amplification nanoparticles loaded with anti-bisphenol A monoclonal antibody by Sumei Ling, Aidi Xu, Menghan Sun, Xiaoli Li, Yongming Huang, Yang Xu, Jianli Huang, Tingting Xie, Shihua Wang

“…The detection result in BPA spiked samples measured by the proposed methods was consistent with that detected by LC-MS method. …”
Get full text

Development of a β-lactamase-based aggregation-induced emission lateral flow strip for the detection of clavulanic acid in Milk by Xiaonan Wang, Tong He, Leina Dou, Licai Ma, Xuezhi Yu, Zhanhui Wang, Kai Wen

“…Lack of biorecognition elements significantly hinders the development of rapid detection methods for clavulanic acid (CA). To address this, we expressed Class A β-lactamases PC1 in vitro and demonstrated its high affinity for CA. …”
Get full text

Automated Detection of Tailing Impoundments in Multi-Sensor High-Resolution Satellite Images Through Advanced Deep Learning Architectures by Lin Qin, Wenyue Song

Get full text

Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL by Hao Gao, Yiling Tian, Min Zhang, Jianhui Liu, Yaowu Yuan, Jianxin Tan, Aijin Ma

“…Due to the shortcomings of the current detection methods, it is extremely urgent to establish a rapid and sensitive detection method for ABL in foods. …”
Get full text

Concurrent detection of circulating minor histocompatibility antigen-specific CD8+ T cells in SCT recipients by combinatorial encoding MHC multimers. by Kelly Broen, Annelies Greupink-Draaisma, Rob Woestenenk, Nicolaas Schaap, Anthony G Brickner, Harry Dolstra

“…Therefore, a set of 75 candidate MiHA peptides was predicted from polymorphic genes with a hematopoietic expression profile and further selected for high and intermediate binding affinity for HLA-A2. Screening of a large cohort of SCT recipients resulted in the detection of dual-color encoded CD8⁺ T cells following MHC multimer-based T cell enrichment and short ex vivo expansion. …”
Get full text

Di-Iron Trioxide Hydrate-Multi-Walled Carbon Nanotube Nanocomposite for Arsenite Detection Using Surface Plasmon Resonance Technique by Yasmin Mustapha Kamil, Sura Hmoud Al-Rekabi, Husam Abduldaem Mohamed, Muhammad Hafiz Abu Bakar, Samikannu Kanagesan, Yap Wing Fen, Mohd Adzir Mahdi

“…We present surface plasmon resonance-based sensor for arsenite [As(III)] detection using Di-iron trioxide hydrate - multi-walled carbon nanotube (Fe₂H₂O₄‐MWCNT) nanocomposite as a sensing layer. …”
Get full text

Development and validation of highly sensitive multiplex real-time RT-PCR assay for detection of classical swine fever virus genome by A. S. Sadchikova, A. S. Igolkin, R. S. Chernyshev, A. A. Kozlov, I. S. Kolbin, A. V. Sprygin, D. A. Biryuchenkov, I. A. Chvala, A. Mazloum

“…Modified primers (locked nucleic acids containing conformationally blocked nucleosides) providing a higher affinity to the DNA matrix and physico-chemical stability and a FAM-labeled TaqMan probe were selected for 5’-untranslatedregion of the genome. …”
Get full text

Highly Sensitive Suspension Immunoassay for Multiplex Detection, Differentiation, and Quantification of Eight <i>Staphylococcus aureus</i> Enterotoxins (SEA to SEI) by Paulin Dettmann, Martin Skiba, Daniel Stern, Jasmin Weisemann, Hans Werner Mages, Nadja Krez, Martin B. Dorner, Sara Schaarschmidt, Marc A. Avondet, Marcus Fulde, Andreas Rummel, Birgit Strommenger, Sven Maurischat, Brigitte G. Dorner

“…This study aimed to develop a highly sensitive and specific multiplex suspension immunoassay (SIA) for SEA to SEI. To this end, high-affinity monoclonal antibodies (mAbs) for the specific detection of the individual SEs were generated. …”
Get full text