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DNA aptamers targeting glycoprotein D enable specific detection of pseudorabies virus (PRV)
Published 2025-04-01“…After 15 selection cycles, Apt-gD-2 demonstrated nanomolar affinity (Kd = 6.107 ± 0.476 nM) and high specificity for gD, as validated by an enzyme-linked aptamer-sorbent assay (ELASA) and fluorescence microscopy. …”
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102
Recent Advances in Aptamer-Based Microfluidic Biosensors for the Isolation, Signal Amplification and Detection of Exosomes
Published 2025-01-01“…Aptamers, which are single-stranded oligonucleotides with high affinity and specificity for target molecules, have been frequently employed in the development of aptamer-based microfluidics for the isolation, signal amplification, and quantitative detection of exosomes. …”
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103
Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions
Published 2025-01-01“…Recently, it was shown that lead ions can also be detected using affinity-based biosensors, namely, using aptamers as recognition elements. …”
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104
Electrochemical Aptamer-Based Biosensor for Detecting Pap31, a Biomarker for Carrion’s Disease
Published 2024-11-01“…Electrochemical aptamer-based (E-AB) biosensors provide a potential solution for this unmet need, as these biosensors are portable, sensitive, and can rapidly report the detection of small molecule targets. Here, we developed an E-AB biosensor to detect Pap31, a biomarker of Carrion’s disease and an outer membrane protein in <i>B. bacilliformis</i>. …”
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105
Dry-printed carbon nanotube film based electrochemical immunosensor for total testosterone detection
Published 2025-10-01“…Limit of detection (LOD) and limit of quantification (LOQ) of 12.7 and 82 ng/dL were achieved respectively. …”
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106
Synergistic Adsorption and Fluorescence in Porous Aromatic Frameworks for Highly Sensitive Detection of Radioactive Uranium
Published 2025-04-01“…At the same time, the fluorescence quenching effect of MIPAF-15 was observed upon its adsorption of uranyl ions, which allowed the selective detection of uranyl ions with a detection limit of 5.04 × 10<sup>−8</sup> M, lower than the maximum concentration of uranyl ions in drinking water specified by the World Health Organization (6.30 × 10<sup>−8</sup> M) and United States Environmental Protection Agency (1.11 × 10<sup>−7</sup> M). …”
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107
Impedimetric Sensor for SARS-CoV-2 Spike Protein Detection: Performance Assessment with an ACE2 Peptide-Mimic/Graphite Interface
Published 2024-12-01“…The COVID-19 pandemic has prompted the need for the development of new biosensors for SARS-CoV-2 detection. Particularly, systems with qualities such as sensitivity, fast detection, appropriate to large-scale analysis, and applicable in situ, avoiding using specific materials or personnel to undergo the test, are highly desirable. …”
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108
A Self-Supervised Feature Point Detection Method for ISAR Images of Space Targets
Published 2025-01-01“…Firstly, this paper simulates an ISAR primitive dataset and uses it to train the proposed basic feature point detection model. Subsequently, the basic feature point detection model and affine transformation are utilized to label pseudo-ground truth for ISAR images of space targets. …”
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109
An innovative biosensing approach for Aflatoxin B1 detection via electrical impedance measurement
Published 2025-06-01“…Aflatoxin B1 (AFB1) contamination poses a significant threat to food safety and public health, creating an urgent need for reliable and effective detection methods. This study presents a fast and sensitive biosensor for detecting AFB1 in complex food samples. …”
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110
Improving Mountainous DSM Accuracy Through an Innovative Opposite-Side Radargrammetry Algorithm
Published 2025-01-01“…The generated DSM is compared against open-source light detection and ranging data from the U.S. Geological Survey. …”
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111
Phylogenetic and Autecology Characteristics of Five Potentially Harmful Dinoflagellate <i>Alexandrium</i> Species (Dinophyceae, Gonyaulacales, Pyrocystaceae) in Tropical Waters: <i...
Published 2025-02-01“…No Paralytic Shellfish Toxins (PSTs) were found in the two <i>Alexandrium affine</i> strains, VINVN01-1 and VINVN01-2. The detection limit for PSTs ranged from 0.45 to 15.5 fg cell<sup>−1</sup>, depending on the molecular response and available biomass.…”
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112
Detection of VOCs and Biogenic Amines Through Luminescent Zn–Salen Complex-Tethered Pyrenyl Arms
Published 2024-12-01“…Fluorescence titrations and density functional theory (DFT) calculations reveal and explain the high binding affinity of this receptor toward selected amines, demonstrating its potential as an effective tool for amine detection.…”
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113
In-House Immunoglobulin Y-Based Immunoassay for Detecting Benzo[a]pyrene in Grilled Pork Samples
Published 2024-12-01“…The objective of this study is to create an immunoassay for the detection of benzo[a]pyrene utilizing immunoglobulin Y antibodies. …”
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114
Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot)
Published 2013-01-01“…Bound thrombin was detected immunoenzymatically using a peroxidase-linked antithrombin antibody and a chromogenic substrate. …”
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Rigid DNA Frameworks Anchored Transistor Enabled Ultrasensitive Detection of Aβ-42 in Serum
Published 2025-05-01“…Here, we develop a graphene field-effect transistor (G-FET) sensor modified with a type of rigid DNA framework aptamer—tetrahedral DNA nanostructure (TDN) for Aβ-42 detection in serum. The Aβ-42 specific aptamer combined with the rigid tetrahedral nanostructure achieves higher binding affinity and better specificity and anti-fouling ability. …”
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Assessing the Effectiveness of Immunoelectric Method in Detecting Mycobacterium avium Subspecies paratuberculosis in Cow Faeces With Paratuberculosis
Published 2025-05-01“…This study aimed to optimize and evaluate an immunoelectric (IE) method for detecting MAP in faecal samples from infected cows. …”
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119
SERS-based detection of DNA methylation for cancer diagnosis: Cation-mediated adsorption to silver nanoparticles.
Published 2025-01-01“…Additionally, the use of SYBR Green fluorescence assays revealed that hypermethylated genomic DNA exhibits greater affinity for silver surfaces compared to lower methylated DNA. …”
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120
A novel method of BiFormer with temporal-spatial characteristics for ECG-based PVC detection
Published 2025-05-01“…IntroductionPremature Ventricular Contractions (PVCs) can be warning signs for serious cardiac conditions, and early detection is essential for preventing complications. …”
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