o8G-modified circKIAA1797 promotes lung cancer development by inhibiting cuproptosis by Haotian Xu, Qingyun Zhao, Dunyu Cai, Xingcai Chen, Xiaodong Zhou, Yihong Gao, Jiaxi Wu, Shengyi Yuan, Deqing Li, Ruirui Zhang, Wenyi Peng, Gang Li, Aruo Nan

“…Methods circRNA differential expression profiles in lung cancer were revealed via RNA high-throughput sequencing, and circKIAA1797 expression in lung cancer cell lines and tissues was detected using qPCR. Experiments such as o8G RNA immunoprecipitation (o8G RIP) and crosslinking immunoprecipitation (CLIP) were performed to explore the presence of o8G on circKIAA1797. …”
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Screening and Molecular Dynamic Simulations of Colicin Produced by Commensals Escherichia coli Isolated from Animals by Amal F. Ghanim, Basil Abbas, Ali B. Al-Deewan, Hanan Hasan Ali

“…E. coli strains isolated from animal feces were subjected to detection of colicin production. The produced colicin was prepared as a disc to serve as an inhibitor for other types of bacteria on Mueller-Hinton agar. …”
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AduCPI2 alleviates MSU-induced acute gouty arthritis in mice by inhibiting cathepsin S and the C5a-C5aR1 axis by Sijing Liu, Sijing Liu, Yongli Situ, Yongli Situ, Li Deng, Li Deng, Meng Liang, Meng Liang, Haoyuan He, Zheng Shao, Zheng Shao, Lifei Peng, Lifei Peng

“…An enzyme activity inhibition assay was conducted to detect the inhibitory effect of CPI2 on cathepsin S. …”
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Capsule network based analysis of histopathological images of oral squamous cell carcinoma by Santisudha Panigrahi, Jayshankar Das, Tripti Swarnkar

“…A major challenge lies in the detection of oral cancer from histopathological images. …”
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Naringenin as a phytogenic adjuvant systematically enhances the protective efficacy of H9N2 inactivated vaccine through coordinated innate-adaptive immune priming in chickens by Lina Jiao, Zuchen Song, Yantong Zhou, Tianyu Zhu, Ruihong Yu, Zheng Wang, Yawei Qiu, Jinfeng Miao, Shun Zhang, Zhenguang Liu, Deyun Wang

“…In this study, we evaluated the adjuvant activity of naringenin (Nar) for H9N2 inactivated vaccine by detecting humoral immunity, cellular immunity, and viral challenge. …”
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Agonist efficacy at the β2AR is driven by the faster association rate of the Gs protein by Clare R. Harwood, Clare R. Harwood, David A. Sykes, David A. Sykes, David A. Sykes, Theo Redfern-Nichols, Owen Underwood, Colin Nicholson, Colin Nicholson, Armin N. Khoshgrudi, Armin N. Khoshgrudi, Eline J. Koers, Eline J. Koers, Graham Ladds, Graham Ladds, Stephen J. Briddon, Stephen J. Briddon, Dmitry B. Veprintsev, Dmitry B. Veprintsev, Dmitry B. Veprintsev

“…In addition, we examined the ability of these β2AR agonists to activate the heterotrimeric Gs protein, measured using the Gs-CASE protein biosensor in living cells. This assay detects a reduction in NanoBRET between the nano-luciferase (nLuc) donor on the Gα subunit and Venus acceptor on the Gγ upon Gs protein activation.ResultsThe 12 β2AR agonists under study revealed a broad range of ligand potency and efficacy values in the cellular Gs-CASE assays. …”
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Crosstalk between the serine/threonine kinase StkP and the response regulator ComE controls the stress response and intracellular survival of Streptococcus pneumoniae. by Germán E Piñas, Nicolás M Reinoso-Vizcaino, Nubia Y Yandar Barahona, Paulo R Cortes, Rosario Duran, Chandan Badapanda, Ankita Rathore, Dario R Bichara, Melina B Cian, Nadia B Olivero, Daniel R Perez, José Echenique

“…Using nonphosphorylatable (ComET128A) and phosphomimetic (ComET128E) proteins, we confirmed that Thr128-phosphorylation increased the DNA-binding affinity of ComE. The non-phosphorylated form of ComE interacted more strongly with StkP than the phosphomimetic form at acidic pH, suggesting that pH facilitated crosstalk. …”
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Protective role of self-assembled nanoparticle vaccine of Pseudomonas aeruginosa in a mouse model of bronchiectasis with acute infection by WU Ziyu, WU Ziyu, ZHANG Yueyue, ZHANG Yiwen

“…The target protein was purified via affinity chromatography and renatured to obtain the desired protein. …”
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MEMBRANE LEc EXPRESSION IN BREAST CANCER CELLS by Ya. A. Udalova, N. V. Bovin, P. S. Obukhova, N. V. Shilova, A. M. Kovrigina, A. A. Subbotina, V. V. Timoshenko, O. E. Galanina, Z. G. Kadagidze, V. P. Letyagin, N. N. Tupitsyn

“…Affine chromatography was used to isolate Lec antibodies from the sera of a healthy female donor with the high titers of these anti- bodies, which were labeled with biotin. …”
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Artificial intelligence-based multimodal model for the identification of ulcerative colitis with concomitant cytomegalovirus colitis by Haozheng Liang, Yuxuan Tian, Gechong Ruan, Xiaoyin Bai, Wei Han, Xiangling Fu, Yuhang Wang, Jialin Shi, Yinghao Sun, Ji Wu, Chenyi Guo, Hong Yang

“…A multimodal dynamic affine transformation (DAFT) model integrating clinical biomarkers and endoscopic images was constructed, along with ResNet and SeNet models. …”
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Shengui Sansheng San alleviates the worsening of blood–brain barrier integrity resulted from delayed tPA administration through VIP/VIPR1 pathway by Jiacheng Hu, Yiyang Li, Xingping Quan, Yan Han, Jinfen Chen, Mengchen Yuan, Ying Chen, Manfei Zhou, Enze Yu, Jiahao Zhou, Dawei Wang, Ruibing Wang, Yonghua Zhao

“…The expressions of VIP, VIPR1, ZO-1, Occludin, Lectin, GFAP, NeuN were detected by immunofluorescence staining or western blotting. …”
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SegAN for Recognition of Caries From 2D-Panoramic X-Ray Images by Parvathaneni Naga Srinivasu, Gorli L. Aruna Kumari, D. Jaya Kumari, Paolo Barsocchi, Akash Kumar Bhoi

“…Data augmentation, like histogram equalization and affine transforms, is performed on the input images for precise segmentation of the samples. …”
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Unraveling mitotic protein networks by 3D multiplexed epitope drug screening by Lorenz J Maier, Stefan M Kallenberger, Katharina Jechow, Marcel Waschow, Roland Eils, Christian Conrad

“…This workflow comprises iterative antibody staining, high‐content 3D imaging, and machine learning for detection of mitoses. This is followed by mapping of spatial protein localization into a spherical, cellular coordinate system, a basis for model‐based prediction of spatially resolved affinities of proteins. …”
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LV-FeatEx: Large Viewpoint-Image Feature Extraction by Yukai Wang, Yinghui Wang, Wenzhuo Li, Yanxing Liang, Liangyi Huang, Xiaojuan Ning

“…Such conditions can result in unreliable feature detection. Consequently, this hinders the performance of vision-based systems. …”
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Robust Optical and SAR Image Matching via Attention-Guided Structural Encoding and Confidence-Aware Filtering by Qi Kang, Jixian Zhang, Guoman Huang, Fei Liu

“…Additionally, a match-aware multi-task loss function jointly enforces spatial consistency, affine invariance, and structural coherence for end-to-end optimization. …”
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Sniffing out chemosensory genes from the Mediterranean fruit fly, Ceratitis capitata. by Paolo Siciliano, Francesca Scolari, Ludvik M Gomulski, Marco Falchetto, Mosè Manni, Paolo Gabrieli, Linda M Field, Jing-Jiang Zhou, Giuliano Gasperi, Anna R Malacrida

“…Chemosensory behaviour plays an important role in many crucial stages in the life of this insect, such as the detection of pheromone cues during mate pursuit and odorants during host plant localisation. …”
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Structure-Based Identification of SARS-CoV-2 nsp10-16 Methyltransferase Inhibitors Using Molecular Dynamics Insights by Ahmad M. Alharbi

“…SARS-CoV-2 evades immune detection via nsp10-16 methyltransferase-mediated 2′-O-methylation of viral mRNA, making it a key antiviral target. …”
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Two decades of Lloviu virus in Europe: knowns and unknowns about the European filovirus by Dr Gábor Kemenesi, Dr Zsófia Lanszki, Ágota Ábrahám, Dr Sándor A Boldogh, Dr Simon Scott, professor Nigel Temperton, Dr Edward Wright, Dr Pierre Nouvellet, Dr Stefania Leopardi, Dr Paola Di Benedictis, Dr Adam Hume, Dr Elke Mühlberger, Levente Sipos-Szabó, Dr Dávid Bajusz, Dr Heliana Dundarova, Dr Szilárd L Bücs, Dr Branka Bajić, Dr Ivana Budinski, Dr Štefan Matis, Dr Tamás Görföl

“…In parallel we analysed the ectoparasite samples with sequencing and histology to get a better understanding about their potential vector role. Results: We detected the viral RNA in all sample types, except oral swabs. …”
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RARE CASE! SECOND PRIMARY MALIGNANCY IN LANGERHANS CELL HISTIOCYTOSIS, A JAK2+ CASE by Engin Yola, Aslı Odabaşı Giden, Caner Çulha, Düzgün Özatlı

“…While it can appear in any organ system, LCH has a particular affinity for bones, skin, lungs, and the pituitary gland. …”
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Network pharmacology and in silico analysis reveal Kochiae Fructus as a potential therapeutic against atopic dermatitis through immunomodulatory pathway interactions. by Shakeel Ahmad Khan

“…Molecular docking demonstrated robust binding affinities of key phytochemicals, particularly ecdysterone and 11,14-eicosadienoic acid, to the anti-AD core targets. …”
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