Chitosan-based multimodal polymeric nanoparticles targeting pancreatic β-cells by Lorenzo Rossi, Cataldo Pignatelli, Krisztina Kerekes, Francesca Cadamuro, András Dinnyés, Felix Lindheimer, Jochen Seissler, Magdalena Lindner, Sibylle Ziegler, Peter Bartenstein, Yi Qiu, Judit Kovács-Kocsi, Zoltán Körhegyi, Magdolna Bodnár, Erika Fazekas, Eszter Prépost, Francesco Nicotra, Laura Russo

“…The detectability of the NPs labeled with IRDye®800CW and Ex4 in MSOT experiments was demonstrated. …”
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Viral sequence determines HLA-E-restricted T cell recognition of hepatitis B surface antigen by Gavuthami Murugesan, Rachel L. Paterson, Rakesh Kulkarni, Veronica Ilkow, Richard J. Suckling, Mary M. Connolly, Vijaykumar Karuppiah, Robert Pengelly, Archana Jadhav, Jose Donoso, Tiaan Heunis, Wilawan Bunjobpol, Gwilym Philips, Kafayat Ololade, Daniel Kay, Anshuk Sarkar, Claire Barber, Ritu Raj, Carole Perot, Tressan Grant, Agatha Treveil, Andrew Walker, Marcin Dembek, Dawn Gibbs-Howe, Miriam Hock, Ricardo J. Carreira, Kate E. Atkin, Lucy Dorrell, Andrew Knox, Sarah Leonard, Mariolina Salio, Luis F. Godinho

“…Furthermore, HLA-E-Env371-379 L6I-specific CD8+ T cells are detectable in HBV-naïve donors and people with chronic HBV after in vitro priming. …”
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Immunogenicity Evaluation of Epitope-Based Vaccine on Target of RNAIII-Activating Protein (TRAP) of <i>Staphylococcus Aureus</i> by Simiao Yu, Di Yao, Xintong Wang, Wei Yu, Yuhua Wei, Wei Liu, Liquan Yu, Jinzhu Ma, Chunyu Tong, Jing Chen, Yongzhong Yu, Baifen Song, Yudong Cui

“…In this study, in silico MHC affinity measurement methods were used to predict possible binding regions, and five 20-mer synthetic TRAP peptides (TRAPP) were synthesized. …”
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Development of a high-throughput TR-FRET assay to identify inhibitors of the FAK-paxillin protein-protein interaction by Rukayat Aromokeye, Martha Ackerman-Berrier, Rosa del Carmen Araujo, Maria Lambousis, Savio Cardoza, L. Charlie Chen, Matthew E. Kaplan, Haining Zhu, Christopher Penton, Celina Zerbinatti, Gregory R.J. Thatcher, Timothy Marlowe

“…The 384-well low volume assay is based on time-resolved fluorescence resonance energy transfer (TR-FRET) technology and uses the high affinity biotin-PEG-1907 stapled peptide to mimic paxillin. …”
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Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System by Nadezhda Shilova, Alexey Nokel, Alexander Lipatnikov, Nailya Khasbiullina, Yuri Knirel, Ludmila Baidakova, Alexander Tuzikov, Sergei Khaidukov, Polina Obukhova, Stephen Henry, Batozhab Shoibonov, Emin Salimov, Robert Rieben, Nicolai Bovin

“…</b> It was found that about 30% of anti-glycan antibodies in human serum detected by the PGA did not activate the complement. …”
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Galangin ameliorates PTU-induced vitiligo in zebrafish and B16F10 cells by increasing melanogenesis through activation of the p38/JNK MAPK pathway by Zulipikaer Wusiman, Zulipikaer Wusiman, Ai-Mei Zhang, Shu-Shu Zhang, Ping-Ping Zhao, Ping-Ping Zhao, Yu-Tong Kang, Yu-Tong Kang, Yun Zhang, Zhi-Jian Li, Zhi-Jian Li, Shi-Xia Huo, Shi-Xia Huo

“…Finally, Western blot was performed to detect the proteins of the relevant pathways.ResultsThe results showed that GA significantly increased melanin production and tyrosinase activity in depigmented zebrafish. …”
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In-silico analysis of deleterious non-synonymous SNPs in the human AVPR1a gene linked to autism by Md. Delowar Kobir Jibon, Md. Asadul Islam, Md. Eram Hosen, Md. Omar Faruqe, Rashed Zaman, Uzzal Kumar Acharjee, Biswanath Sikdar, Yewulsew Kebede Tiruneh, Md. Khalekuzzaman, Motasim Jawi, Magdi E. A. Zaki

“…We employed several bioinformatics tools, such as SNPnexus, PROVEAN, PANTHER, PhD-SNP, SNP & GO, and I-Mutant2.0, to detect the 23 most detrimental mutants (R85H, D202N, E54G, H92P, D148Y, C203G, V297M, D148V, S182N, Q108L, R149C, G212V, M145T, G212S, Y140S, F207V, Q108H, W219G, R284W, L93F, P156R, F136C, P107L). …”
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Assessment of changes in synaptic density in the zQ175DN mouse model of Huntington’s disease: a [18F]SynVesT-1 study by Liesbeth Everix, Franziska Zajicek, Annemie Van Eetveldt, Longbin Liu, Jonathan Bard, Steven Staelens, Daniele Bertoglio

“…SV2A can readily be quantified via positron emission tomography (PET) using the selective and high affinity SV2A radiotracer [18F]SynVesT-1 that we previously characterized in C57BL/6J mice. …”
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Identificación Varietal de 41 Plantas Seleccionadas de Cacao (Theobroma cacao L.) Provenientes de Cuatro Cultivares Distintos de la Región Amazónica Ecuatoriana, Mediante el Uso de... by Christian Abraham Romero Bonifaz, Julio Andrés Bonilla, Efrén Germán Santos Ordóñez, Esther Lilia Peralta García

“…The average genetic diversity detected among the individuals within each of the four populations was He = 0.80. …”
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Epitope mapping targeting the K205R protein of African swine fever virus using nanobody as a novel tool by Xuedan Wei, Fengxia Zhang, Qiming Pei, Aijuan Shen, Duoxing Niu, Yaci Zhang, Ziheng Zhang, Yunshuo Lu, Angke Zhang, Gaiping Zhang, Hong Duan

“…Nanobodies exhibit superior capability in detecting concealed epitopes of antigens compared with traditional antibodies. …”
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Identificación Varietal de 41 Plantas Seleccionadas de Cacao (Theobroma cacao L.) Provenientes de Cuatro Cultivares Distintos de la Región Amazónica Ecuatoriana, Mediante el Uso de... by Christian Abraham Romero Bonifaz, Julio Andrés Bonilla, Efrén Germán Santos Ordóñez, Esther Lilia Peralta García

“…The average genetic diversity detected among the individuals within each of the four populations was He = 0.80. …”
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Identificación Varietal de 41 Plantas Seleccionadas de Cacao (Theobroma cacao L.) Provenientes de Cuatro Cultivares Distintos de la Región Amazónica Ecuatoriana, Mediante el Uso de... by Christian Abraham Romero Bonifaz, Julio Andrés Bonilla, Efrén Germán Santos Ordóñez, Esther Lilia Peralta García

“…The average genetic diversity detected among the individuals within each of the four populations was He = 0.80. …”
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Quercetin inhibits ferroptosis to alleviate intestinal ischemia-reperfusion injury by MA Xiaojie, LENG Yufang, LENG Yufang, MU Jialu

“…Western blotting was utilized to detect the protein expression of glutathione peroxidase 4 （GPX4）， acyl-CoA synthetase long-chain family member 4 （ACSL4）， and tumor protein 53 （p53）. …”
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Application of bioinformatics analysis and molecular docking to study the mechanism of Qingying decoction in treating psoriasis by Cuicui Shen, Xuewei Liu, Huangchao Jia, Wenhe Wang, Xiaomeng Wang, Haiyan Wang, Dan Wang, Jianwei Li

“…Molecular docking was used to detect the binding ability between key components and hub targets. …”
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Effect and mechanism of modified Yougui power on Simmental bulls with oligoasthenozoospermia based on targeted amino acid metabolism by Baoxia Chen, Qiang Ma, Huifang Ma, Wenfei Zhang, Runmin Wu, Chun Niu, Rongxia Guo, Zhiyuan Ma, Peng Ji, Yanming Wei, Yongli Hua

“…The levels of amino acid metabolites and related metabolic pathways in semen of the OA and control groups were detected by UHPLC-MRM-MS/MS. Molecular docking was used to assess the affinity between the primary active ingredients associated with OA and their core targets.ResultsThe main components of MYP include trehalose, morroniside, hypaconitine, loganin, quercetin, kaempferol, and other compounds. …”
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Keratinocyte-derived VEGF-A is an essential pro-migratory autocrine mediator, acting through the KDR/GEF-H1/RhoA pathway by Vida Maksimoska, Vida Maksimoska, Qinghong Dan, Neetu Rambharack, Neetu Rambharack, Katalin Szászi, Katalin Szászi, Katalin Szászi, Katalin Szászi

“…RhoA and GEF-H1 activation were detected using affinity precipitation assays with GST-RBD or GST-RhoA (G17A). …”
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Low Antibody-Dependent Enhancement of Viral Entry Activity Supports the Safety of Inactivated SARS-CoV-2 Vaccines by Xiaofang Peng, Yuru Han, Song Xue, Yunjiao Zhou, Weiyu Jiang, Anqi Xia, Wei Wu, Yidan Gao, Fan Wu, Qiao Wang

“…Results: Our analysis revealed no significant difference in binding affinity or neutralizing capacity between the vaccinated and convalescent mAbs. …”
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The overview and role of heat shock proteins (HSP) especially HSP 60 and 70 in reproduction and other pathologies (a literature review) by V.O. Berestoviy, Ahmad Mahmood, I.B. Venckivska, V.G. Ginzburg, I.V. Sokol, O.O. Berestoviy, D.O. Govsieiev

“…ELISA, western blotting, immunofluorescence, and affinity chromatography were the most common methods researchers used to determine and separate HSP 60 and antibodies related to it. …”
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Therapeutic effect of dihydroartemisinin on Alzheimer’s disease model mice with senile macular degeneration by Gao Han, Gong Xuewu, Zhang Meng, Wang Yuejing, Wang Yuchun, Zhang Keshuang, Yao Hongbo

“…Morris water maze was used to detect the spatial learning and memory ability of AD mice. …”
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Phenotypic and genotypic characterization of antimicrobial resistance and virulence profiles of Salmonella enterica serotypes isolated from necropsied horses in Kentucky by Ajran Kabir, William G. Kelley, Cheyenne Glover, Erdal Erol, Yosra A. Helmy

“…The genotypic antimicrobial resistance (AMR) and virulence profiles were detected using polymerase chain reaction (PCR). The overall prevalence of Salmonella was 1.19% (26/2182), with 11 different serotypes identified. …”
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