Development and validation of novel biomarker assays for osteoarthritis. by Khadija Ourradi, Yunhe Xu, Dominique de Seny, John Kirwan, Ashley Blom, Mohammed Sharif

“…<h4>Method</h4>Monoclonal and polyclonal antibodies were generated by immunising mouse and rabbits respectively with peptide-carrier conjugates of C3f and V65. Affinity purified antibodies were used for immunoassays development and assays validated using serum from OA patients and controls.…”
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A Study of CO Emission Sources and Their Impacts on the  Respiratory Systems of  Patients in Al-Muthanna Governorate by Farah Ali, Ali Abdulhamza Al -Fanharawi

“…Control areas had no detectable CO (0.00 ppm). Health assessments included blood (WBC, Lym, Neu, Eos), lung function (FVC, FEV, PEF), and immunological markers (IgE).  …”
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Activity cliff-aware reinforcement learning for de novo drug design by Xiuyuan Hu, Guoqing Liu, Yang Zhao, Hao Zhang

“…Experimental evaluations across multiple protein targets demonstrate ACARL’s superior performance in generating high-affinity molecules compared to existing state-of-the-art algorithms. …”
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Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins. by Chenyao Ruan, Chen Wang, Jiawen Gu, Zhihui Zhu

“…MST results showed that there was a strong affinity between isoscopoletin and GPD2, GPI, Hsp90α and PGK2. …”
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mAb Das-1 recognizes 3'-Sulfated Lewis A/C, which is aberrantly expressed during metaplastic and oncogenic transformation of several gastrointestinal Epithelia. by Jeffrey W Brown, Koushik K Das, Vasilios Kalas, Kiron M Das, Jason C Mills

“…The epitope recognized by mAb Das-1 was determined by affinity to a comprehensive glycan array and validated by inhibition of a direct ELISA.…”
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Frizzled-7-targeting antibody-derived bifunctional protein retargets NK cells against triple-negative breast cancer cells via MICA-NKG2D axis by Lin Yang, Tong Wang, Wei Xie, Jin Wang, Jing Xu, Hao Yang, Gang Huang, Haiyan Xie, Xiaofei Zhang, Danfang Li, Xingxing Li, Qingqing Huang, Chenyue Wang, Lisha Wu, Shuyang Mao, Zitong Wang, Yuxia Liu

“…To reestablish MICA/NKG2D-mediated immunosurveillance, we proposed the design of a fusion protein (SHH002-hu1-MICA) which consists of Frizzled-7 (Fzd7)-targeting antibody and MICA, serving as an engager retargeting NK cells against TNBCs, especially TNBCSCs.Methods Opal multicolor immunohistochemistry staining was used to validate the expression of membrane MICA (mMICA) and existence of NK cells in TNBC tumors; flow cytometry (FCM) assay was used to detect the expression of Fzd7/mMICA on TNBCs. Biolayer interferometry (BLI) and surface plasmon resonance (SPR) assays were executed to assess the affinity of SHH002-hu1-MICA towards rhFzd7/rhNKG2D; near-infrared imaging assay was used to evaluate the targeting capability. …”
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Characterization, effect of metal ions and organophosphates on the brain acetylcholinesterase of Nile Tilapia (Oreochromis niloticus) in vitro by ALLYSSON K. DE CARVALHO SILVA, FERNANDA JENIFFER L. LIMA, SUZANE MERIELY DA S. DUARTE, MARLLEN S. DA SILVA, CAROLINA B. CORDEIRO, ULISSES M. NASCIMENTO, WENER M.T. DOS SANTOS, MARIA DO D.S.B. NASCIMENTO, TALITA DA S. ESPÓSITO, ANTÔNIO CARLOS R. BORGES

“…AChE exhibited high substrate affinity, with a Vmax of 0.651 ± 0.26 mU/mg and a Michaelis-Menten constant (Km) of 399 mU/mg. …”
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PLATELET-MEDIATED PLASMINOGEN PROCESSING PRODUCES ANGIOSTATINS: AN IMMUNOCHEMICAL STUDY by L.G. Kapustianenko, O.I. Yusova, V.V. Korsa, T.V. Grinenko

“…Plasminogen and its fragments were detected by immunoblot with the use of previously obtained polyclonal antibodies to plasminogen kringles (K1-3 and K5). …”
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Somatic mutations in intracranial arteriovenous malformations. by Jeremy A Goss, August Y Huang, Edward Smith, Dennis J Konczyk, Patrick J Smits, Christopher L Sudduth, Christopher Stapleton, Aman Patel, Sanda Alexandrescu, Matthew L Warman, Arin K Greene

“…Patient and AVM characteristics were recorded.<h4>Results</h4>We detected somatic mutations in 10 of 16 specimens (63%). …”
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Clinico-genomic study reveals association of dengue virus genome high frequency mutations with dengue disease severity by Varsha Ravi, Md Imran, Kriti Khare, Pallavi Mishra, Ramakant Mohite, Kanika, Md Abuzar Khan, Aparna Swaminathan, Aanchal Yadav, Sristi Sinha, Richa Shukla, Partha Chattopadhyay, Jyoti Soni, Ranjeet Maurya, Tavpritesh Sethi, Bansidhar Tarai, Sandeep Budhiraja, Rajesh Pandey

“…The findings from the associated clinical data with the variants of the DENV are critical for early detection of the disease and understanding the disease progression. …”
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Dual-labeled anti-GD2 targeted probe for intraoperative molecular imaging of neuroblastoma by Lauren Taylor Rosenblum, ReidAnn E. Sever, Ryan Gilbert, David Guerrero, Sarah R. Vincze, Dominic M. Menendez, Peggy A. Birikorang, Mikayla R. Rodgers, Ambika Parmar Jaswal, Alexander C. Vanover, Joseph D. Latoche, Angel G. Cortez, Kathryn E. Day, Lesley M. Foley, Chaim T. Sneiderman, Itay Raphael, T. Kevin Hitchens, Jessie R. Nedrow, Gary Kohanbash, W. Barry Edwards, Marcus M. Malek

“…Probe accumulation in xenografts was detected and visualized using widely available operative tools (Neoprobe® and SPY-PHI camera) and facilitated detection ofputative residual disease in the resection cavity following unguided resection. …”
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Occurrence, Distribution, and Ecological Risk Assessment of Antibiotics in Selected Urban Lakes of Hanoi, Vietnam by Hong Anh Duong, Thi Vi Phung, Thuy Ngoc Nguyen, Lan-Anh Phan Thi, Hung Viet Pham

“…While the highest concentration of SMX was detected at 806.5 ng/L in surface water, those obtained in sediment were only at 1.35 ng/g because of their high solubility in water. …”
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Seroepidemiology for <i>Orthorubulavirus suis</i> in Mexican Pigs by Development of an Indirect ELISA Based on a Recombinant NP Protein by Rocío Lara-Romero, José Luis Cerriteño-Sánchez, María Azucena Castañeda-Montes, Humberto Ramírez-Mendoza, Julieta Sandra Cuevas-Romero

“…In this study, the recombinant protein NP of LPMV was produced in the <i>E. coli</i> BL21 system and then purified using affinity chromatography. The purified protein was used to coat plates for an indirect ELISA assay (iELISA). …”
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Resistance of <i>Alternaria</i> spp. Causing Strawberry Black Spot to Boscalid in China by Tao Li, Wenbin Yu, Ji Feng, Chengxin Mao, Hong Yu, Aichun Liu, Chuanqing Zhang

“…Molecular docking analysis revealed that the SDHC-H134R mutation reduced the binding affinity between boscalid and mitochondrial complex II. …”
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Understanding the impacts of dual methionine oxidations in complementarity-determining regions on the structure of monoclonal antibodies by Bo Zhao, Joy Yoon, Bojie Zhang, Youmi Moon, Yue Fu, Yinyin Li, Yunlong Zhao, Hui Xiao, Ning Li

“…It is imperative to fully understand the effects of methionine oxidation because these modifications can affect the binding affinity, stability, and immunogenicity of mAbs. Moreover, the presence of multiple methionines in close proximity within the amino acid sequence increases the complexity of accurate characterization, and sophisticated analytical methods are required to detect these modifications. …”
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PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model by Volkan Tekin, Yujun Zhang, Clayton Yates, Jesse Jaynes, Henry Lopez, Charles Garvin, Benjamin M. Larimer, Suzanne E. Lapi

“…In contrast, the healthy mice exhibited no detectable CD206 staining, lower PET signals, and reduced radiopharmaceutical accumulation in lung tissues at the same timepoint. …”
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Insight into molecular and mutational scrutiny of epilepsy associated gene Gabrg2 leading to novel computer-aided drug designing by Muhammad Naveed, Nimra Hanif, Tariq Aziz, Muhammad Waseem, Metab Alharbi, Abdulrahman Alshammari, Abdullah F. Alasmari

“…Epilepsy-causing gene GABRG2 was selected, and the gene was then examined to detect the presence of polymorphic markers with the help of molecular (PCR) and computational analysis. 50 blood samples of epilepsy patients were collected from Children hospital Lahore, out of which 2 samples were shown positive response for GABRG2 gene, indicating that this gene is becoming the cause of seizures in epilepsy patients. …”
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The FMRF-NH2 gated sodium channel of Biomphalaria glabrata: Localization and expression following infection by Schistosoma mansoni. by Laura C Vicente-Rodríguez, Amanda C Torres-Arroyo, Anthony Hernández-Vázquez, Mariela Rosa-Casillas, Dina P Bracho-Rincón, Paola Méndez de Jesús, Martine L Behra, Mohamed R Habib, Xiao-Nong Zhou, Joshua J C Rosenthal, Mark W Miller

“…In common with the P. trivolvis FaNaC, the B. glabrata channel exhibited a low affinity (EC50: 3 x 10-4 M) and high specificity for the FMRF-NH2 agonist. …”
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Biochemical characterization and activity profiling of recombinant phospholipase A2 from Hemiscorpius lepturus expressed in E. coli with in vivo antibody response by Moslem Najafi, Delavar Shahbazzadeh, Parichehreh Yaghmaie, Hasan Mirzahoseini

“…The PLA2 gene was cloned into pET-26b (+), expressed in E. coli BL21 (DE3) pLysS, and purified by affinity chromatography. The secondary structure of the recombinant protein was analyzed using CD spectroscopy. …”
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Integrating computational approaches to uncover β-lactamase-associated resistance in diarrheagenic Escherichia coli from pediatric patients by Taru Singh, Chandra Shekhar, Saurabh Singh, Shukla Das

“…Molecular docking revealed that while amoxicillin had stronger binding affinity for certain enzymes, ceftriaxone demonstrated greater inhibitory potency across key targets. …”
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