Subtelomeric repeat expansion in Hydractinia symbiolongicarpus chromosomes by Tetsuo Kon, Koto Kon-Nanjo, Oleg Simakov

“…Based on the sequence divergence, its partial affinity with the Helitron group can be detected. This sequence is associated with a repeated minisatellite unit of about 150 bp. …”
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Parallel Mutations Result in a Wide Range of Cooperation and Community Consequences in a Two-Species Bacterial Consortium. by Sarah M Douglas, Lon M Chubiz, William R Harcombe, F Marty Ytreberg, Christopher J Marx

“…Non-synonymous mutations in metA, encoding homoserine trans-succinylase (HTS), were detected in each evolved S. enterica methionine cooperator and were shown to be necessary for cooperative consortia growth. …”
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Solubilization and Display of G Protein-Coupled Receptors on Beads for Real-Time Fluorescence and Flow Cytometric Analysis by L.A. Sklar, J. Vilven, E. Lynam, D. Neldon, T.A. Bennett, E. Prossnitz

“…However, the G protein reconstitution was not detected on the beads, apparently for steric reasons. …”
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Colorectal cancer vaccines: in silico identification of tumor-specific antigens associated with frequent HLA-I alleles in the costa rican Central Valley population by Diego Morazán-Fernández, José Arturo Molina-Mora

“…A variant calling analysis was implemented to detect tumorspecific SNVs, in comparison to healthy tissue. …”
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Morphological and genetic polymorphism of the introduced population of <i>Prunus pumila</i> L. in Chelyabinsk Province by M. S. Lezin, S. V. Asbaganov

“…In individual cases, a weak connection of separate markers with leaf blade edge serration, stone surface structure, plant habitus, and geographic origin of the genotype was detected. Concurrent inclusion of all primers and genotypes in the cluster analysis failed to group the studied P. pumila samples into clades according to the presence/absence of any traits, which may attest to their close genetic affinity. …”
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Biochemical and biophysical investigations of the interaction between human glucokinase and pro-apoptotic BAD. by Alix Rexford, Diego A R Zorio, Brian G Miller

“…Furthermore, fluorescence polarization assays and isothermal titration calorimetry detect no direct interaction between GCK and BAD BH3 peptides. …”
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Flow cytometry for the analysis of α-dystroglycan glycosylation in fibroblasts from patients with dystroglycanopathies. by Elizabeth Stevens, Silvia Torelli, Lucy Feng, Rahul Phadke, Maggie C Walter, Peter Schneiderat, Ayad Eddaoudi, Caroline A Sewry, Francesco Muntoni

“…Immunocytochemistry and immunoblotting are two of the most widely used methods to detect the amount of α-DG glycosylation in muscle. …”
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PINK1-Interacting Proteins: Proteomic Analysis of Overexpressed PINK1 by Aleksandar Rakovic, Anne Grünewald, Lisa Voges, Sarah Hofmann, Slobodanka Orolicki, Katja Lohmann, Christine Klein

“…Recent publications suggest that the Parkinson's disease- (PD-) related PINK1/Parkin pathway promotes elimination of dysfunctional mitochondria by autophagy. We used tandem affinity purification (TAP), SDS-PAGE, and mass spectrometry as a first step towards identification of possible substrates for PINK1. …”
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The Distinct Biological Effects of 6-Hydroxy-L-Nicotine in Representative Cancer Cell Lines by Paula Alexandra Postu, Razvan Stefan Boiangiu, Marius Mihasan, Alexandru Bogdan Stache, Adrian Tiron, Lucian Hritcu

“…In silico analyses were conducted to evaluate the binding affinity of 6HLN to nicotinic receptors (nAChRs) containing α9 and α5 subunits. …”
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Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies by Pavana Suresh, Sun Zhen-Yu Jim, Ishani Sahay, Allison Y. Tang, Charlotte Wijne, Jin Gan, Novalia Pishesha, Jose L. Avalos, Hidde Ploegh

“…VHH1-4SMT3 bind to Smt3p and Smt3p-tagged proteins with high affinity (Kd: low nM). NMR analysis shows that the four nanobodies all bind near the C-terminus of Smt3p, partially overlapping with the binding site for the SUMO protease Ulp1p. …”
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Impact of holder pasteurization on protein and eNAMPT/Visfatin content in human breast milk by Annalisa Givonetti, Chiara Galantin, Irene Fiorilla, Alberto Maria Todesco, Michela Braghin, Elena Uga, Gianluca Cosi, Valentina Audrito, Maria Cavaletto

“…Few investigations have been reported about detecting visfatin in skimmed milk and the hypothesis of its potential role in regulating infant adiposity through breast milk. …”
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Verteporfin Inhibits Severe Fever with Thrombocytopenia Syndrome Virus Infection via Inducing the Degradation of the Viral Gn Protein by Bingan Wu, Chenyang Yu, Yuxiang Lin, Ping Zhao, Zhongtian Qi, Xijing Qian

“…The potential targets of VP were detected by a drug affinity responsive target stability (DARTS) assay. …”
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Modeling an evolutionary conserved circadian cis-element. by Eric R Paquet, Guillaume Rey, Felix Naef

“…This fragment shows a signal involving the presence of two closely spaced E-box-like motifs, a configuration that we can also detect in the other four prominent CLK/CYC target genes in flies: timeless, vrille, Pdp1, and cwo. …”
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Investigation of biometabolites and novel antimicrobial peptides derived from promising source Cordyceps militaris and effect of non-small cell lung cancer genes computationally. by Muhammad Afzal, Mai Abdel Haleem A Abusalah, Neelum Shehzadi, Muhammad Absar, Naveed Ahmed, Sarmir Khan, Yalnaz Naseem, Noshaba Mehmood, Kirnpal Kaur Banga Singh

“…Indolizine, 2-(4-methylphenyl) has most binding affinity (micromolar) and optimized properties to be selected as the lead inhibitor. …”
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Differential expression and subcellular localization of BmHMGA in silkworm (Bombyx mori) by Zhang Haihua, Zhao Yan, Zhang Hanming, Li Si, Quan Yanping, Yu Wei, Zhang Yaozhou, Tong Fudan, Zhang Xiaojuan

“…New Zealand rabbits were immunized with fusion protein purified by N-i NTA affinity chromatography and the polyclonal antibody was obtained. …”
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A novel hybrid approach to overcome defects of CE-SELEX and cell-SELEX in developing aptamers against aspartate β-hydroxylase by Hadi Bakhtiari, Hamed Naghoosi, Sina Sattari, Mahmoud Vahidi, Mehdi Shakouri Khomartash, Ali Faridfar, Mohsen Rajaeinejad, Mohsen Nikandish

“…Here, we introduced a kind of hybrid CE-Cell-SELEX, named CEC hybrid-SELEX, for addressing these limitations in creating aptamer probes detecting human aspartate β-hydroxylase (ASPH), which is a well-established tumor biomarker, in cancer diagnostic investigations. …”
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Anticancer Effect of Pinostrobin on Human Breast Cancer Cells Through Regulation of Epithelial Mesenchymal Transition by Pimrapat Jongjang MSc, Sutharinee Likitnukul PhD, Somrudee Reabroi PhD, Supachoke Mangmool PhD, Bodee Nutho PhD, Darawan Pinthong PhD

“…Wound healing and Transwell chamber assays were conducted to examine the effect of pinostrobin on migration ability. RT-PCR was used to detect the expression of mRNA involved in NF-κB and EMT signaling pathways. …”
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Evaluation of the immunogenic property of NT H. influenzae protein D with Neisseria meningitidis OMV in BALB/c by Ava Behrouzi, Saeid Bouzari, Seyed Davar Siadat, Mana Oloomi, Mehdi Davari, Hoora Mazaheri

“…In order to purify the protein, Ni-NTA agarose was used to perform affinity chromatography. Purified PD and PD mixed with outer membrane vesicle (OMV) and alum adjuvant were used for subcutaneous immunization in BALB/c mice. …”
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Social network analysis in humanitarian logistics research by Zhasmina Tacheva, Natalie Simpson

“…Network motifs suggesting distinct affinity between particular agencies within humanitarian clusters are observed. …”
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STUDY OF THE PECULIARITIES OF THE EVOLUTION OF KARYOTYPE BASED ON THE ANALYSIS OF PHYLOGENETIC RELATIONS OF THE ACRIDIDAE FAMILY by Ольга Николаевна Гуляева, Людмила Васильевна Высоцкая

“…The mutual increase in the centromeric index of the first and the second chromosomes in these species was detected. Conclusions. The phylogenetic affinity of the Euchorthippus pulvinatus species from the Gomphocerini tribe with the characteristic representatives of the Chrysochraontini tribe was revealed which indicates that during the karyotypic evolution of the representatives of these tribes, Robertson’s confluences included different chromosomes. …”
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