Genome-wide association study of rubella virus vaccine strain BRD-Ⅱ by XU Yingmei, ZHU Yongqiang, ZHOU Xin, LIU Zhaoyang, MA Leijun, CHEN Zhewen, ZHAO Yueye, ZHU Tiaoxia

“…ObjectiveTo perform a genome-wide association study of rubella virus vaccine strain BRD-Ⅱ, so as to fully grasp the sequence characteristics of this genome.MethodsSecond-generation sequencing method was used to conduct the whole-genome sequencing on the vaccine strain BRD-Ⅱ, and the affinity tree of this genome with some vaccine strains and wild-type rubella virus strains was analyzed using the maximum likelihood method. …”
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Production of recombinant norovirus VP1 protein and its antigenic and immunogenic properties by Vladislav A. Lapin, Dmitry V. Novikov, Ekaterina V. Mokhonova, Dmitry A. Melentyev, Maria I. Tsyganova, Dmitry E. Zaitsev, Viktor V. Novikov

“…Computer analysis of nucleotide and amino acid sequences, molecular cloning, polymerase chain reaction, electrophoresis of nucleic acids in agarose gel and proteins in polacrylamide gel, affinity chromatography, enzyme immunoassay. Results and discussion. …”
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Preparation of recombinant myoglobin and investigation of the liquid antigen stability for quality control materials by Yu-Hui Wang, Xi-Feng Sun, Chun-Xin Xu, Feng-Qiang Sun, Rong-Rong Wang, Xiao-Kun Bian, Zhan-Zhao Wang, Qiang Wu

“…It was a promising alternative as the quality control material for Mb detection.…”
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Genetic diversity of sarbecoviruses isolated from microbats in Southern Japan by Kosuke Okuya, Sho Sata, Mana Esaki, Isshu Kojima, Masahiro Kajihara, Shin Murakami, Takuji Sakata, Kimitake Funakoshi, Makoto Ozawa

“…These deletions attenuate the viral binding affinity to human angiotensin-converting enzyme 2 (ACE2) receptors. …”
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High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application by Niu LIU, Jun CHE, Duo LAI, Jin-jun WEN, Han-hong XU

“…The results suggested that it has a potential application in the detection of pesticide residues.…”
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RNA guanine content and G-quadruplex structure tune the phase behavior and material properties of biomolecular condensates by Alfredo Vidal Ceballos, Anna Geissmann, Denize C. Favaro, Priyasha Deshpande, Shana Elbaum-Garfinkle

“…Additionally, we found that while methylation of FMRP decreased RNA binding affinity, this modification did not impact condensate material properties suggesting that RNA sequence/structure can play a greater role than binding affinity in determining the emergent properties of condensates. …”
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Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. by Aziza P Manceur, Wei Zou, Anne Marcil, Eric Paquet, Christine Gadoury, Bozena Jaentschke, Xuguang Li, Emma Petiot, Yves Durocher, Jason Baardsnes, Manuel Rosa-Calatrava, Sven Ansorge, Amine A Kamen

“…Finally, a simulation model was created to predict the binding affinity of the two anti-HA antibodies to the amino acids composing the highly conserved epitope; different probabilities of interaction between a given amino acid and the antibodies might explain the affinity of each antibody against different influenza strains.…”
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RETRACTED ARTICLE: Structure based innovative approach to analyze aptaprobe–GPC3 complexes in hepatocellular carcinoma by Woo-Ri Shin, Dae-Young Park, Ji Hun Kim, Jin-Pyo Lee, Nguyen Quang Thai, In-Hwan Oh, Simranjeet Singh Sekhon, Wooil Choi, Sung Yeon Kim, Byung-Kwan Cho, Sun Chang Kim, Jiho Min, Ji-Young Ahn, Yang-Hoon Kim

“…Conclusion Aptaprobe has the potential to be used as an affinity reagent to detect the target in vivo and in vitro diagnosing system. …”
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Deciphering UBE4B phosphorylation dynamics: a key mechanism in p53 accumulation and cancer cell response to DNA damage by Yasser Abuetabh, H. Helena Wu, Habib Al Yousef, Sujata Persad, Mary-Pat Schlosser, David D. Eisenstat, Consolato M. Sergi, Roger Leng

“…Abstract The p53 tumor suppressor protein plays a crucial role in detecting and eliminating various oncogenic threats by promoting processes such as cell cycle arrest, DNA repair, senescence, and apoptosis. …”
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Combined treatment with anti-PSMA antibody and human peripheral blood-derived NK cells for castration-resistant prostate cancer by Fangming Wang, Nianzeng Xing, Jianxing Li

“…The expression of CD107a and CD16 (the Ab Fc-receptor) in NK cells and the Ab affinity were detected using flow cytometry. Antitumor effects were evaluated in patient-derived organoid (PDO) models and in 22RV1 tumor-bearing mice in vivo.ResultsWe constructed an anti-PSMA Ab and validated its high affinity toward the PSMA antigen. …”
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Syphilitic infection: a study on the factors of the innate humoral immune response by N. K. Levchik, N. V. Zilberberg, M. V. Ponomareva, O. A. Belykh

“…B1 cells produce antibodies that bind with low affinity to microbial structures common to various pathogens. …”
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HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats. by Osiris Marroquin Belaunzaran, Sascha Kleber, Stefan Schauer, Martin Hausmann, Flora Nicholls, Maries Van den Broek, Sravan Payeli, Adrian Ciurea, Simon Milling, Frank Stenner, Jackie Shaw, Simon Kollnberger, Paul Bowness, Ulf Petrausch, Christoph Renner

“…The presence of B272 can be detected on leukocytes of HLA-B27+ Ankylosing spondylitis (AS) patients and HLA-B27 transgenic rats. …”
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Ergotamine Targets KIF5A to Facilitate Anoikis in Lung Adenocarcinoma by Bin Bao, Xiaojun Yu, Wujun Zheng, Jiewei Sun

“…The small molecular drug with the highest affinity for KIF5A was screened out through molecular docking experiments, which was validated through cellular thermal shift assay (CETSA). …”
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Metal‐Free Raman Sensing Platforms of Organic Nanowire Arrays Produced by High Energy Charged Particles by Masaki Nobuoka, Shugo Sakaguchi, Yusuke Tsutsui, Akie Taguchi, Makito Takagi, Tomomi Shimazaki, Masanori Tachikawa, Akira Idesaki, Tetsuya Yamaki, Devesh Kumar Avasthi, Merry Gupta, Ramcharan Meena, Ambuj Tripathi, Shu Seki

“…The designed electron transfer reactions between the analytes and nanowires provide Raman signal enhancement factors of up to 108 with the detection limit of the analytes as 10−9 M for the rhodamine, indicating the viability of these ONAs as a novel class of metal‐free Raman sensing probes.…”
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Unlocking the potential of beta sitosterol: Augmenting the suppression of oral cancer cells through extrinsic and intrinsic signalling mechanisms by Selvaraj Jayaraman, Sathan Raj Natarajan, Bhuvaneswari Ponnusamy, Vishnu Priya Veeraraghavan, Sharmila Jasmine

“…Additionally, docking results were employed to validate the findings demonstrating high binding affinity of β-sitosterol with apoptotic-mediated signalling targets. …”
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Network pharmacology and experimental verification to investigate the mechanism of isoliquiritigenin for the treatment of Alzheimer’s disease by Waimao Gao, Guang Yang, Xinjuan Liu, Kaifan Hu, Jie Pan, Xingyu Wang, Yan Zhao, Ying Xu

“…The protein-protein interaction (PPI) network was constructed and clusters were obtained using the Molecular Complex Detection (MCODE) and the Cytohubba plugins. Further, molecular docking study was performed for these core targets. …”
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A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma by Xiang Guo, Xiang Guo, Xiang Guo, Congfang Guo, Dongxiao Li, Yuting Bai, Mureed Abbas, Mureed Abbas, Ruiwen Fan, Yiyan Zhao, Yiyan Zhao

“…This NKG2A-targeting nanobody demonstrates the potential for application in both the detection and treatment of MM.…”
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Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies against Coronavirus SARS-CоV-2 by G. V. Kuklina, S. S. Ipatov, A. S. Gorshkov, D. V. Pechenkin, A. V. Eremkin, A. V. Kuznetsovsky, A. S. Tumanov, I. V. Darmov

“…Monoclonal antibodies were purified by affinity chromatography on protein A sepharose sorbent, conjugated with horseradish peroxidase, and tested for the possibility to be used in sandwich ELISA for detection of inactivated SARS‑CoV‑2 coronavirus strain “Isolate B”. …”
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Anti-citrullinated histone monoclonal antibody CIT-013, a dual action therapeutic for neutrophil extracellular trap-associated autoimmune diseases by Maarten van der Linden, Sangeeta Kumari, Daphne Montizaan, Stephanie van Dalen, Annemarie Kip, Martyn Foster, Inge Reinieren-Beeren, Elsa Neubert, Luise Erpenbeck, Kelsy Waaijenberg, Tirza Bruurmijn, Rezie te Poele, Peter van Zandvoort, Paul Vink, Eric Meldrum, Helmuth van Es, Renato G.S. Chirivi

“…Here, we describe a first-in-class monoclonal antibody (CIT-013) with high affinity for citrullinated histones H2A and H4, which inhibits NETosis and reduces tissue NET burden in vivo with significant anti-inflammatory consequences. …”
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LPA₃: Pharmacodynamic Differences Between Lysophosphatidic Acid and Oleoyl-Methoxy Glycerophosphothionate: Biased Agonism, Two Sites by K. Helivier Solís, M. Teresa Romero-Ávila, Ruth Rincón-Heredia, Juan Carlos Martínez-Morales, J. Adolfo García-Sáinz

“…These findings suggest that two binding sites for these agonists might exist in the LPA₃ receptor, one showing a very high affinity for OMPT and another likely shared by LPA and OMPT (structural analogs) with lower affinity.…”
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