‘High hopes for treatment’: Australian stakeholder perspectives of the clinical translation of advanced neurotherapeutics for rare neurological diseases by Christina Q. Nguyen, Didu S. T. Kariyawasam, Tsz Shun Jason Ngai, James Nguyen, Kristine Alba‐Concepcion, Sarah E. Grattan, Elizabeth E. Palmer, Kate Hetherington, Claire E. Wakefield, Russell C. Dale, Sue Woolfenden, Shekeeb Mohammad, Michelle A. Farrar

“…However, health literacy, perceptions and understanding of novel therapies need elucidation across the RND community. This study explored healthcare professionals' and carers' perspectives of advanced therapies in childhood‐onset RNDs. …”
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Machine learning identifies genes linked to neurological disorders induced by equine encephalitis viruses, traumatic brain injuries, and organophosphorus nerve agents by Liduo Yin, Morgen VanderGiessen, Morgen VanderGiessen, Vinoth Kumar, Benjamin Conacher, Po-Chien Haku Chao, Michelle Theus, Erik Johnson, Kylene Kehn-Hall, Kylene Kehn-Hall, Xiaowei Wu, Hehuang Xie

“…Gene ontology and pathway analyses further identified neuropathologic features with specific gene product attributes and functions, shedding light on the fundamental biology of these neurological disorders. …”
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Over 100 years of longitudinal connectivity changes from the perspective of a migratory fish species by Johannes Luca Kowal, Gertrud Haidvogl, Andrea Funk, Johannes Schützenhofer, Paulo Branco, Maria-Teresa Ferreira, Paul Meulenbroek, Rafaela Schinegger, Thomas Hein

“…For this purpose, a graph theoretic approach is deployed that integrates structural and functional aspects, illustrating connectivity changes from the perspective of a characteristic migratory fish species Chondrostoma nasus (Linnaeus 1758). …”
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The Symbiotic Bacterial Profile of Laboratory-Reared and Field-Caught <i>Aedes albopictus</i> Mosquitoes from Greece by Elias Asimakis, Ioannis Galiatsatos, Georgia Apostolopoulou, Eleni C. Savvidou, Georgios Balatsos, Vasileios Karras, Vasiliki Evangelou, Eva Dionyssopoulou, Antonios Augustinos, Nikos T. Papadopoulos, Antonios Michaelakis, Panagiota Stathopoulou, George Tsiamis

“…However, they retain core elements shared across populations that may exhibit valuable nutritional or functional roles and could be exploited to improve SIT processes.…”
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PCI-DB: a novel primary tissue immunopeptidome database to guide next-generation peptide-based immunotherapy development by Sven Nahnsen, Hans-Georg Rammensee, Cécile Gouttefangeas, Jonas S Heitmann, Juliane S Walz, Jens Bauer, Marcel Wacker, Annika Nelde, Jonas Scheid, Steffen Lemke, Marissa L Dubbelaar, Patrick Zimmermann, Naomi Hoenisch Gravel, Susanne Jung, Anna Dengler, Yacine Maringer, Sven Fillinger, Tatjana Bilich

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Unveiling migraine subtype heterogeneity and risk loci: integrated genome-wide association study and single-cell transcriptomics discovery by Shuxu Wei, Yan Quan, Xinyi Li, Suiqin Zhong, Ling Xiao, Chao Yang, Ronghuai Shen, Xiaojia Lu, Lingbin He, Youti Zhang, Xianxi Huang

“…But they showed divergent functional architectures in functional genomic annotations, with MA enriched in conserved regulatory elements (e.g., Backgrd_Selection_StatL2_0, enrichment = 1.38, P = 5.47 × 10−6) and MO in vascular pathways (e.g., GERP.NSL2_0, enrichment = 2.12, P = 1.04 × 10−6). …”
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Milk and cheesemaking whey as a sustainable source of extracellular vesicles: Exploring large-scale isolation methods with industrial and potential therapeutic applications by Andrea del Saz-Lara, María-Carmen López de las Hazas, João Tomé-Carneiro, Carmen Mazarío-Gárgoles, Judit Gil-Zamorano, Livia Balaguer, Lorena del Pozo-Acebo, Antonio González-Sarrías, María Ángeles Ávila-Gálvez, Juan Carlos Espín, Diego Gómez-Coronado, Francesco Visioli, Alberto Dávalos

“…We systematically compared isolation techniques including UC, tangential flow filtration (TFF), and polyethylene glycol (PEG) co-precipitation across milk and whey from bovine, caprine, and ovine sources. …”
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Machine learning–guided single-cell multiomics uncovers GDF15-driven immunosuppressive niches in NSCLC: A translational framework for overcoming anti-PD-1 resistance by Xianfei Zhang, Zhengxin Yin, Xueyu Chen, Nengchong Zhang, Shengjia Yu, Congcong Zhu, Lianggang Zhu, Liulan Shao, Bin Li, Runsen Jin, Hecheng Li

“…Comparative evaluation of 22 survival algorithms across four NSCLC cohorts (n=156) led to the development of an Accelerated Oblique Random Survival Forest model, which outperformed conventional Cox regression and deep learning methods in predictive accuracy (training C-index=0.864; test C-index=0.748). …”
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Multi-omics identification of a polyamine metabolism related signature for hepatocellular carcinoma and revealing tumor microenvironment characteristics by Yuexi Yu, Huiru Liu, Kaipeng Liu, Meiqi Zhao, Yiyan Zhang, Runci Jiang, Fengmei Wang

“…Utilizing 101 machine learning algorithms, we constructed a polyamine metabolism-related signature (PMRS) and validated its predictive power across training, testing, and external validation cohorts. …”
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Genome-Wide Identification and Characterization of the mTERF Gene Family in Spinach and the Role of <i>SomTERF5</i> in Response to Heat Stress by Ziyue Sun, Li Li, Yaqi Liu, Yanshuang Liu, Gaojian Li, Yueyue Li, Qingbo Yu, Meihong Sun, Xiaofeng Xu

“…In this study, we systematically identified 31 <i>SomTERF</i> genes from the spinach genome, which are distributed across five chromosomes and nine unassembled genomic scaffolds. …”
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Orchard variable-rate spraying method integrating GNSS and wind-excited audio-conducted leaf area density by Hangxing Zhao, Hangxing Zhao, Hangxing Zhao, Shenghui Yang, Shenghui Yang, Shenghui Yang, Wenwei Li, Wenwei Li, Han Feng, Han Feng, Shijie Jiang, Shijie Jiang, Weihong Liu, Weihong Liu, Jingbin Li, Yongjun Zheng, Yongjun Zheng, Songchao Zhang

“…As a result, few fully functional variable-rate spraying systems have been developed based on this parameter.MethodsThis study presents a variable-rate spraying method that integrates global navigation satellite system (GNSS) positioning with wind-excited audio-conducted estimation of canopy leaf area density. …”
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Distinct mRNA expression profiles and miRNA regulators of the PI3K/AKT/mTOR pathway in breast cancer: insights into tumor progression and therapeutic targets by Tomasz Sirek, Tomasz Sirek, Katarzyna Król-Jatręga, Katarzyna Król-Jatręga, Przemysław Borawski, Nikola Zmarzły, Dariusz Boroń, Dariusz Boroń, Dariusz Boroń, Dariusz Boroń, Piotr Ossowski, Olga Nowotny-Czupryna, Kacper Boroń, Dominika Janiszewska-Bil, Elżbieta Mitka-Krysiak, Beniamin Oskar Grabarek, Beniamin Oskar Grabarek

“…Statistical analysis was performed with a statistical significance threshold (p) &lt; 0.05.ResultsWe identified several upregulated genes across all subtypes, with TNBC and HER2-positive cancers showing the most significant changes. …”
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Validation of the European Oncology toolkit for the self-assessment of Quality of Life (EUonQoL-Kit) in cancer patients and survivors: study protocol of a pan European survey by G. Apolone, M. Costantini, L. Caselli, N. Bos, A. Caraceni, G. Ciliberto, N. Couespel, M. Ferrer, M. Groenvold, S. Kaasa, C. Lombardo, R. Pietrobon, G. Pravettoni, A. Sirven, H. Vachon, G. Velikova, O. Garin, A. Gilbert, A. Machiavelli, C. Marzorati, R. Miceli, M. Pe, MA. Petersen, A. Tanzilli, F. van Schelven, C. Dantas, I Minnée-van Braak, L. Pinnavaia, C. Brunelli

“…Discussion The EUonQoL-Kit will serve to systematically incorporate patient perspectives into European cancer policies and to address HRQoL inequalities across Europe. Trial registration ClinicalTrials.gov Identifier: NCT05947903, 2023–06-28.…”
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Impact of methyl Jasmonate on blueberry ripening fruits: assessment of cell wall thermal stability, nutritional parameters and antioxidant enzymatic activity by Carlos Vasquez-Rojas, Carlos Vasquez-Rojas, Marcelo Muñoz-Vera, Sebastián Flores, Mauricio Betancourt, Mauricio Betancourt, Ricardo I. Castro, Patricio Ramos, Patricio Ramos, Daniel Laporte, Carolina Parra-Palma, Luis Morales-Quintana

“…This work provides a preliminary framework for integrating MeJA into sustainable horticultural practices to meet consumer demand for high-quality functional fruits.…”
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Reproductive resilience and trade-offs: Egg component allocation under nutritional constraints in Japanese Quail by Gebrehaweria K. Reda, Sawadi F. Ndunguru, Renáta Knop, James K. Lugata, Brigitta Csernus, Gabriella Gulyás, Csaba Szabó, Ádám Z. Lendvai, Levente Czeglédi

“…However, yolk mass, yolk color, egg shape index, and shell density remained unaffected across all dietary levels. Severe restrictions also increased the yolk ratio while reducing the albumen ratio, suggesting that birds may evolutionarily prioritize yolk deposition to sustain reproductive functions. …”
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Who gets referred for knee or hip replacement? A theoretical model of the potential impact of evidence-based referral thresholds using data from a retrospective review of clinic re... by Andrew J Price, Andrew Judge, Alastair Gray, Rachael Gooberman-Hill, David Beard, Adrian Sayers, Rob Middleton, Stephanie Smith, Karen L Barker, David Murray, Sujin Kang, Elena Benedetto, James Smith, Ray Fitzpatrick, Helen A Dakin, Peter Eibich, Nigel Arden, Andy Carr, Kristina Harris, Elizabeth Gibbons, Jill Dawson, Laura Miller, Elsa Marques, Ashley Blom

“…Those referred typically have low OHS/OKS, and introducing evidence-based OKS/OHS thresholds would prevent few inappropriate (high-functioning, low-pain) referrals. However, our findings suggest that some patients not currently referred could benefit from arthroplasty based on OKS/OHS. …”
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Ether lipids and sphingolipids drive sex-specific human aging dynamics by Joaquim Sol, Anna Fernàndez-Bernal, Natalia Mota-Martorell, Meritxell Martín-Garí, Èlia Obis, Alba Juanes, Victoria Ayala, Jordi Mayneris-Perxachs, Rafel Ramos, Víctor Pineda, Josep Garre-Olmo, Manuel Portero-Otín, José Manuel Fernández-Real, Josep Puig, Mariona Jové, Reinald Pamplona

“…Lipid enrichment analyses highlight acylcarnitines, sphingolipids and ether-linked phospholipids as key contributors, with functional indices indicating compositional shifts in lipid species.These findings suggest an impairment of lipid functional categories, including loss of dynamic properties, alterations in bioenergetics, antioxidant defense, cellular identity, and signaling platforms. …”
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Psychometric properties and meaningful change thresholds for the QOL-E instrument in patients with myelodysplastic neoplasms by Esther Natalie Oliva, Shien Guo, Jennifer Lord-Bessen, Aylin Yucel, Roberto Latagliata, Massimo Breccia, Giuseppe A. Palumbo, Grazia Sanpaolo, Marta Riva, Valeria Santini, Uwe Platzbecker, Guillermo Garcia-Manero, Pierre Fenaux, Christopher G. Pelligra

“…Test-retest reliability and internal consistency was confirmed with intraclass correlation coefficients and Cronbach alpha exceeding 0.70 across most QOL-E domains/summary scores. The QOL-E domains/summary scores, except for QOL-SEX, had an adequate ability to detect change from baseline to Week 24. …”
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TEIPP-vaccination in checkpoint-resistant non-small cell lung cancer: a first-in-human phase I/II dose-escalation study by Mitchell Emmers, Marij J. P. Welters, Michelle V. Dietz, Saskia J. Santegoets, Sanne Boekesteijn, Anouk Stolk, Nikki M. Loof, Daphne W. Dumoulin, Annemarie L. Geel, Lauri C. Steinbusch, A. Rob P. M. Valentijn, Danielle Cohen, Noel F. C. C. de Miranda, Egbert F. Smit, Hans Gelderblom, Thorbald van Hall, Joachim G. Aerts, Sjoerd H. van der Burg

“…Abstract Functional loss of the intracellular peptide Transporter associated with Antigen Processing (TAP) fosters resistance to T-cell based immunotherapy. …”
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Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction by Norma Cecilia Morales-Elias, Eleazar Martínez-Barajas, Lilia Angélica Bernal-Gracida, Monserrat Vázquez-Sánchez, Iris Grisel Galván-Escobedo, Jose Salud Rodriguez-Zavala, Amelia López-Herrera, Cecilia Beatriz Peña-Valdivia, Antonio García-Esteva, Carlos Alberto Cruz-Cruz, María Teresa González-Arnao, José Cruz Jiménez-Galindo, Daniel Padilla-Chacón

“…The fluorescent sucrose analog esculin indicated that transport across the plasma membrane might contribute to the increase in the pith cell diameter of pedicels. …”
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