Large-scale computational modelling of H5 influenza variants against HA1-neutralising antibodiesResearch in context by Colby T. Ford, Shirish Yasa, Khaled Obeid, Rafael Jaimes, III, Phillip J. Tomezsko, Sayal Guirales-Medrano, Richard Allen White, III, Daniel Janies

“…We analysed binding affinity changes over time and across various antibodies using multiple biochemical and biophysical binding metrics. …”
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The Influence of the Divergent Substrate on Physicochemical Properties and Metabolite Profiling of <i>Agrocybe cylindracea</i> Cultivation by Hatungimana Mediatrice, Nsanzinshuti Aimable, Irambona Claude, Nyummah Fallah, Menna-Allah E. Abdelkader, Jules Biregeya, Yingping Hu, Lili Zhang, Hengyou Zhou, Jing Li, Penghu Liu, Zhanxi Lin, Dongmei Lin

“…<i>Agrocybe cylindracea</i> is an important mushroom highly valued as a functional food for its nutritional and medicinal benefits. …”
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A Comprehensive Quality Evaluation System for Medicinal Leeches by Integrating Macromolecular Protein Analysis and Small-Molecule Marker Detection as Well as Quantitative Bioassays by Wenduan Wang, Yufei Liu, Wenjiao Lou, Liangmian Chen, Tianze Xie, Zhimin Wang, Yue Ma, Huimin Gao

“…This strategy combines chemical characterization of small and macromolecular components with bioassays relevant to its clinical functions. A total of 28 batches of samples were analyzed, comprising 23 genuine and 5 counterfeit batches. …”
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Ferro-aluminosilicate composite generated from coal gasification fine slag for the preconcentration and adsorptive removal of selected artificial sweeteners from water by Xoliswa Cingo, Azile Nqombolo, Buhle Xakalashe, Xolisa Goso, Luthando Nyaba, Odwa Mapazi, Philiswa Nosizo Nomngongo

“…Furthermore, converting CGFS into functional adsorbents aligns seamlessly with the principles of a circular economy, emphasising resource reutilization and sustainability. …”
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FlyPhoneDB2: A computational framework for analyzing cell-cell communication in Drosophila scRNA-seq data integrating AlphaFold-multimer predictions by Mujeeb Qadiri, Ying Liu, Ah-Ram Kim, Myeonghoon Han, Eric Zhou, Austin Veal, Tzu-Chiao Lu, Hongjie Li, Yanhui Hu, Norbert Perrimon

“…In addition, the algorithm has been optimized for improved performance and more effective noise filtering. New functionalities have also been introduced, such as the addition of downstream reporter genes to evaluate pathway activity, multi-sample CCC comparison, and enhanced visualizations summarizing communication at a network level.We demonstrate the utility of FlyPhoneDB2 by analyzing whole-body single-nuclei RNA-seq datasets from flies with gut tumors induced by the Yorkie oncogene. …”
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Targeted temperature management at 33 versus 36 degrees after out-of-hospital cardiac arrest: A follow-up study by Robert Doerning, Kyle R. Danielson, Jane Hall, Catherine R. Counts, Michael R. Sayre, Sarah Wahlster, James A. Town, Sue Scruggs, David J. Carlbom, Nicholas J. Johnson

“…Aim: Targeted temperature management (TTM) is commonly used in the setting of out-of-hospital cardiac arrest (OHCA) to improve survival and functional outcomes. However, real-world evidence of effects and optimal temperature are limited. …”
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Genome-Wide Characterization of the <i>ANN</i> Gene Family in <i>Corydalis saxicola</i> Bunting and the Role of CsANN1 in Dehydrocavidine Biosynthesis by Han Liu, Jing Wang, Zhaodi Wen, Mei Qin, Ying Lu, Lirong Huang, Xialian Ou, Liang Kang, Cui Li, Ming Lei, Zhanjiang Zhang

“…This study sheds light on the profiles and functions of the <i>CsANN</i> gene family and paves the way for unraveling the molecular mechanism of BIA accumulation, which is regulated by Ca²⁺ through CsANNs.…”
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Dry cacao pulp in chocolate bars: A sustainable, nutrient-rich sweetener with enhanced sensory quality through refractance windows drying by Idania Quintero, Amandine Ceccaldi, Hugo Martínez, Margareth Santander, Jader Rodríguez, Sebastián Escobar

“…The drying process was studied under three operational temperatures (70, 80, and 90°C) and pulp thicknesses (1, 2, and 3 mm) until reaching an equilibrium moisture content of 0,12 g water/g solid across all conditions. Preservation of the nutritional and functional composition of the dried cacao pulp was assessed through measurements of phenolic compounds, vitamin C, colour, and sugar content. …”
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A Multicenter Retrospective Study Evaluating Distal Radial Access vs. Conventional Transradial or Transvenous Access for Endovascular Treatment of Malfunctioning Dialysis Fistulas by Roberto Minici, Massimo Venturini, Giuseppe Guzzardi, Federico Fontana, Andrea Coppola, Filippo Piacentino, Marco Spinetta, Davide Costa, Maria Chiara Brunese, Pasquale Guerriero, Biagio Apollonio, MGJR Research Team, Nicola De Rosi, Raffaele Serra, Domenico Laganà

“…Methods: A retrospective multicenter study was conducted across four hospitals, including 292 patients treated between January 2019 and June 2024. …”
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Transcriptomic and Metabolomic Analysis Reveals Multifaceted Impact of Gcn5 Knockdown in <i>Drosophila</i> Development by Youfeng Li, Yue Xu, Ruike Li, Sirui Huang, Qiong Wu, Jing Yan, Zhigang Jiang, Xiushan Wu, Fang Li, Yuequn Wang, Yongqing Li, Xiongwei Fan, Wuzhou Yuan

“…<b>Background:</b> General control nonderepressible 5 (Gcn5) is a lysine acetyltransferase (KAT) that is evolutionarily conserved across eukaryotes, with two homologs (Kat2a and Kat2b) identified in humans and one (Gcn5) in <i>Drosophila</i>. …”
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Comparative Analysis of Fully Guided and Free-Hand Orthognathic Surgery: Advancements, Precision, and Clinical Outcomes by Sophia Tsokkou, Ioannis Konstantinidis, Antonios Keramas, Georgios Kiosis, Kanellos Skourtsidis, Danai Alexiou, Georgia-Nektaria Keskesiadou, Sofia Karachrysafi, Theodora Papamitsou, Ioannis Chatzistefanou

“…<b>Methods</b>: A scoping review was conducted across PubMed, MEDLINE, Scopus, Cochrane Library, and Embase databases, focusing on clinical trials and cohort studies. …”
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Paddy soil fertility shifts microbial metabolic quotient by regulating the selective enrichment of specific bacterial groups by Yali Kong, Jie Wang, Chunquan Zhu, Wenhao Tian, Lianfeng Zhu, Xiaochuang Cao, Yupei Chen, Xiaoxia Liu, Haimin Kong, Yijun Yu, Gengmiao Zhang, Junhua Zhang

“…The heterogeneous distribution of specific microbial taxa with diverse physiological traits, caused by changes of soil fertility level, controls community-level functions. However, how specific microbial taxa mediate community-level carbon (C) metabolic efficiency across various fertility conditions in paddy soils is limited. …”
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Privacy-preserving record linkage by a federated trusted third party (fTTP) – unlocking medical research potential in Germany by Hampf, Christopher, Bialke, Martin, Hund, Hauke, Fegeler, Christian, Lang, Stefan, Penndorf, Peter, Wöller, Nico, Moser, Frank-Michael, Blumentritt, Arne, Schuldt, Ronny, Seidel, Florian, Brunecker, Peter, Wettstein, Reto, Arnecke, Lukas, Hoffmann, Wolfgang

“…Existing tools such as identity and pseudonym management systems were expanded regarding their respective functionalities. Results: The fTTP enables PPRL and pseudonymization of patient records. …”
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The m6A modification of LINC01133 suppresses ER+ breast cancer progression by modulating IGF2BP2 protein stability via a ubiquitination-dependent mechanism by Mai-dong Li, Mai-dong Li, Ben-jie Shan, Lei Wang, Shuang Gao, Li Hao, Hai-yang Yu, Yue-yin Pan, Yue-yin Pan

“…LINC01133 exhibits opposing expressing patterns across different breast cancer subtypes, yet its roles and mechanisms in ER+ breast cancer remain a loaded question.MethodsThe expression of LINC01133 was initially assessed utilizing a public dataset TCGA and subsequently validated within clinical samples through RT-qPCR and in situ hybridization (ISH). …”
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Characterization of Change in Cognition Before and After COVID-19 Infection in Essential Workers at Midlife by Zennur Sekendiz, Olga Morozova, Melissa A. Carr, Ashley Fontana, Nikhil Mehta, Alina Ali, Eugene Jiang, Tesleem Babalola, Sean A.P. Clouston, Benjamin J. Luft

“…Cognitive performance across four domains was measured before and after the pandemic. …”
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Age-related differences in the weighting of kinematic and contextual information during action prediction by Elisa Ravizzotti, Alessandra Finisguerra, Gaia Bonassi, Carola Cosentino, Susanna Mezzarobba, Susanna Mezzarobba, Alessandro Botta, Martina Putzolu, Sara Terranova, Valentina Bianco, Valentina Bianco, Laura Avanzino, Laura Avanzino, Elisa Pelosin, Elisa Pelosin, Cosimo Urgesi, Cosimo Urgesi

“…While it is well known that motor and cognitive functions face age-related changes, research examining how action prediction abilities evolve across the lifespan remains limited.MethodsHere, we compared the action prediction performance of 30 young and 30 older adults in a temporal occlusion paradigm displaying everyday actions embedded in breakfast scenarios. …”
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Subthalamic nucleus deep brain stimulation for cranial-cervical dystonia: optimal stimulation sites and networks by Hutao Xie, Jiansong Huang, Ningfei Li, Houyou Fan, Shihang Yang, Zixiao Yin, Zhaoting Zheng, Zehua Zhao, Yin Jiang, Lin Shi, Anchao Yang, Fangang Meng, Guanyu Zhu, Quan Zhang, Jianguo Zhang

“…However, clinical responses vary substantially across individuals, likely due to differences in electrode placement and modulation of target neural circuits. …”
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Oxytocin enhances oligodendrocyte development and improves social deficits in autistic rats by Min Wen, Min Wen, Min Wen, Min Wen, Shuang Zheng, Shuang Zheng, Shuang Zheng, Hongbo Luo, Yi Zhang, Yi Zhang, Yi Zhang, Bo Zhou, Bo Zhou, Bo Zhou

“…Pathway analysis indicated that this functional inhibition was associated with the PI3K/AKT signaling pathway. …”
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Behavioral and neural effects of temporoparietal high-definition transcranial direct current stimulation in logopenic variant primary progressive aphasia: a preliminary study by Elias D. Granadillo, Elias D. Granadillo, Mason Fellmeth, Vahab Youssofzadeh, Joseph Heffernan, Priyanka P. Shah-Basak, Sara B. Pillay, Candida Ustine, Peter Kraegel, Shelby Schold, Kimberly D. Mueller, Kimberly D. Mueller, Chrysanthy Ikonomidou, Ozioma Okonkwo, Manoj Raghavan, Jeffrey R. Binder

“…Changes in resting-state functional connectivity within the phonological network were analyzed using fMRI. …”
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Metabolic capabilities are highly conserved among human nasal-associated Corynebacterium species in pangenomic analyses by Tommy H. Tran, Isabel F. Escapa, Ari Q. Roberts, Wei Gao, Abiola C. Obawemimo, Julia A. Segre, Heidi H. Kong, Sean Conlan, Matthew S. Kelly, Katherine P. Lemon

“…ABSTRACT Corynebacterium species are globally ubiquitous in human nasal microbiota across the lifespan. Moreover, nasal microbiota profiles typified by higher relative abundances of Corynebacterium are often positively associated with health. …”
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