Epidemiological investigation of poultry infectious in Kazakhstan (2021–2024) by Karlygash B. Zikibayeva, Asset A. Svanbayev, Nurlan N. Akhmetsadykov, Kamshat N. Kudaibergenova, Shynar N. Akhmetsadykova, Ernur N. Nurolda, Aidyn I. Kydyrmanov

“…Data sources included official reports, laboratory diagnostics (RT-PCR, ELISA, and sequencing), and a survey of veterinary specialists. …”
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KLF5 silencing restrains proliferation, invasion, migration and angiogenesis of gallbladder carcinoma cells by transcriptional regulation of PDGFA by Xiaowei Lu, Kui Hu, Dandan Zhang, Xuefeng Yin, Jifeng Nie, Kai Zhao

“…Angiogenesis was assessed by ELISA, tube formation assay and western blot. The interaction between KLF5 and PDGFA was confirmed by ChIP assay, as well as luciferase reporter assay. …”
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Staphylococcus aureus induces mitophagy via the HDAC11/IL10 pathway to sustain intracellular survival by Yaji Yang, Haotian Zhou, Feilong Li, Yanhao Zhang, Jianye Yang, Yidong Shen, Ning Hu, Quanming Zou, Leilei Qin, Hao Zeng, Wei Huang

“…Methods This study employed confocal microscopy, flow cytometry, ELISA, and siRNA technology to assess the survival capacity of S. aureus within macrophages and the impact of inflammatory cytokines on its persistence. …”
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Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways by Liang Zhu, Yanchi Bi, Ting Liang, Po Zhang, Xiao Xiao, Tengbo Yu

“…GK's anti-inflammatory and antioxidative effects were comprehensively assessed via MTT assay, western blot, cell immunofluorescence, ELISA, and transcriptome sequencing. Potential underlying mechanisms were also explored. …”
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Diagnostics and treatment of Clostridium difficile-associated disease: Guidelines of the Russian gastroenterological association by V. T. Ivashkin, N. D. Yushchuk, I. V. Mayev, Tatyana L Lapina, Ye. A. Poluektova, O. S. Shifrin, A. S. Tertychny, A. S. Trukhmanov, A. A. Sheptulin, Ye. K. Baranskaya, O. S. Lyashenko, K. V. Ivashkin

“…At the present time several laboratory methods for diagnosis of Clostridial infection exist: fecal ELISA test for toxins A and B, PCR, test for C. difficile glutamate dehydrogenase. …”
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Selecting the Best Music for the Moment in a Music and Imagery Session by Petra Jerling, Carmen Angulo Sánchez-Prieto, Isabel Solana Rubio

“… Music and Imagery (MI) is a receptive music therapy method within the Continuum Model of Guided Imagery and Music developed by Lisa Summer. In receptive music therapy, clients listen to music for therapeutic purposes. …”
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Developing a risk score using liquid biopsy biomarkers for selecting Immunotherapy responders and stratifying disease progression risk in metastatic melanoma patients by Amalia Azzariti, Simona De Summa, Tommaso M. Marvulli, Ivana De Risi, Giuseppe De Palma, Roberta Di Fonte, Rossella Fasano, Simona Serratì, Sabino Strippoli, Letizia Porcelli, Michele Guida

“…Methods Soluble forms of PD1, PD-L1, LAG-3, CTLA-4, CD4, CD73, and CD74 were quantified using ELISA assay in plasma of a cohort of 110 MM patients, at baseline, to investigate possible correlations with clinical outcomes. …”
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Revealing viral hepatitis epidemiology in the Democratic Republic of Congo: insights from yellow fever surveillance reanalysis by Patrick Mukadi-Kakoni, Yannick Munyeku-Bazitama, Gracia Kashitu-Mujinga, Marguerite Manwana-Pemba, Niclette Zenga-Bibi, Patient Okitale-Talunda, Christelle Mbelu-Kabongo, Fleurette Domai-Mbuyakala, Elisabeth Pukuta-Simbu, Pierre Mutantu-Nsele, Yoshinao Kubo, Sheila Makiala-Mandanda, Steve Ahuka-Mundeke, Koya Ariyoshi, Jean-Jacques Muyembe-Tamfum

“…Samples from individuals testing negative for yellow fever were tested for IgM antibodies against HAV, HBc, HCV, and HEV and HBs antigen using validated ELISA kits. Acute HBV infection was defined by both HBc IgM and HBs antigen positivity. …”
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The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind,... by Mehran Noori, Zainab Shateri, Siavash Babajafari, Mohammad Hadi Eskandari, Karim Parastouei, Mohammad Ghasemi, Hoseein Afshari, Mohammad Samadi

“…Oxidative stress parameters were assessed using the standard calorimetric method, and inflammatory parameters were measured via the enzyme-linked immunosorbent assay method (ELISA). The differences between the two groups were compared using the independent samples T-test. …”
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The diagnostic performance evaluation of the SD BIOLINE HIV/syphilis Duo rapid test in southern Ethiopia: a cross-sectional study by Techalew Shimelis, Endale Tadesse

“…Where the result of the STAT-PAK is discordant with KHB, Unigold HIV is used as a tiebreaker to determine the result. We also used ELISA to resolve discordant HIV results. Syphilis serostatus was determined using the Treponema pallidum haemagglutination assay (TPHA).Results The respective sensitivity, specificity, positive predictive value and negative predictive value of the SD BIOLINE HIV/syphilis Duo test were 100, 99.5, 99.5 and 100% for HIV and 97.6, 96, 95.4 and 98% for syphilis testing, respectively. …”
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Ficus deltoidea standardized methanolic extract improves kidney structure and function against streptozotocin-induced diabetic nephropathy in rats by Nurdiana Samsulrizal, Noor Syaffinaz Noor Mohamad Zin, Nur Syimal`ain Azmi, Mohd Daud Bakar, Hafandi Ahmad, Yong-Meng Goh

“…Enzyme-linked immunosorbent assays (ELISA) assessed the levels of oxidative stress, antioxidants, and apoptosis-related proteins in the kidney tissue. …”
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Effect of rhGH treatment on lipidome and brown fat activity in prepuberal small for gestational age children: a pilot study by Lorena González, Carolina Gonzalez-Riano, Pablo Fernández-García, Rubén Cereijo, Aina Valls, Andrea Soria-Gondek, Nativitat Real, Belén Requena, Joan Bel-Comos, Patricia Corrales, David Jiménez-Pavón, Coral Barbas, Francesc Villarroya, David Sánchez-Infantes, Marta Murillo

“…Untargeted lipidomics analysis was performed using liquid chromatography-mass spectrometry (LC-MS), and serum biomarker levels were measured using ELISA assays. Serum lipidomic analysis revealed that free fatty acids (FFAs) increased to levels close to those of the AGA group after three months of rhGH administration, including polyunsaturated fatty acids, correlating with reduced leptin levels. …”
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Effect of remote ischemic preconditioning on perioperative neurocognitive disorder in elderly patients undergoing major surgery and associated genetic variant analysis: a randomize... by Feifei Xu, Tingting Liu, Huiqing Liu, Jiao Deng, Shan He, Zhihong Lu, Haopeng Zhang, Hailong Dong

“…Serum levels of myeloid differentiation factor 2 (MD2) and Cystatin C and PND-associated single nucleotide polymorphisms were analyzed by ELISA and whole genome sequencing, respectively. …”
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The status of serum 25(OH)D levels is related to breast cancer by Mohammad Momivand, Mahta Razaghi, Farshid Mohammadi, Edris Hoseinzadeh, Roya Najafi-Vosough

“…D levels were measured using the ELISA method. Statistical analysis was performed using SPSS version 26, with a significance threshold set at a 95% confidence level. …”
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Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway by Zhuming Chen, Jing Lu, Tianying Chang, Dongmei Zhang, Yibin Zhang, Miao Liu, Tong Wu, Peng Xv, Jian Wang

“…Flow cytometry was utilized to count Th17, Th1, Th2, and Treg cells in lymphocytes. ELISA and RT-qPCR were used to measure acetylcholine receptor antibody (AChR-Ab) and Th17-related cytokines, including IL-17, IL-21, IL-23, TNF-α, TGF-β, IL-1β, and IL-6. …”
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Targeting CXCL8 signaling sensitizes HNSCC to anlotinib by reducing tumor-associated macrophage-derived CLU by Xin Hu, Yikang Ji, Mi Zhang, Zhihui Li, Xinhua Pan, Zhen Zhang, Xu Wang

“…In vivo and in vitro CXCL8 and CLU expression levels in tumor cells were determined using qPCR, immunohistochemistry and ELISA. The ability of CLU from TAMs to respond to tumor-derived CXCL8 was assessed using RNA sequencing, siRNA silencing, immunofluorescence and CCK-8 assays. …”
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Identifying Priority Areas for Increasing the Supply of Medication-Assisted Treatments for Opioid Use Disorder: A Geospatial Approach by Michael Topmiller, Peter J. Mallow, Aaron T. Vissman, Jene Grandmont

“…Analyses included standard deviation outlier mapping, local indicators of spatial autocorrelation (LISA), and map overlays. **Results:** We identified twenty-nine opioid dependence priority areas, eleven unmet treatment need priority areas, and seven low MAT capacity priority areas, located across the US, including southeastern Ohio, western Indiana, the District of Columbia, New England, and northern and southern California. …”
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Targeting sphingosine 1-phosphate receptor 3 inhibits T-cell exhaustion and regulates recruitment of proinflammatory macrophages to improve antitumor efficacy of CAR-T cells agains... by Weiting Liao, Ge Gao, Xia He, Pei Shu, Qizhi Ma, Benxia Zhang, Diyuan Qin, Yongsheng Wang

“…We have applied RNA sequencing, flow cytometry, ELISA, cellular/molecular immunological technology, and mouse models of solid cancers.Results Our study provided evidence that S1PR3 high expression is positively associated with resistance to programmed cell death protein-1 (PD-1)-based immunotherapy and increased T-cell exhaustion. …”
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Ultrasensitive interferons quantification reveals different cytokine profile secretion in inflammatory myopathies and can serve as biomarkers of activity in dermatomyositis by Loïs Bolko, Loïs Bolko, Loïs Bolko, Céline Anquetil, Céline Anquetil, Alba Llibre, Solène Maillard, Damien Amelin, Karim Dorgham, Vincent Bondet, Océane Landon-Cardinal, Océane Landon-Cardinal, Océane Landon-Cardinal, Ségolène Toquet, Ségolène Toquet, Kuberaka Mariampillai, Samuel Malatre, Alexandrine Mahoudeau, Baptiste Hervier, Mathieu Rodero, Guy Gorochov, Darragh Duffy, Olivier Benveniste, Olivier Benveniste, Yves Allenbach, Yves Allenbach

“…Serum IFN-β level was measured by Elisa. Correlation between IFN levels and disease activity were performed.ResultsWe included 242 IIM patients and found a good correlation between type I Interferon (IFN) and dermatomyositis disease activity. …”
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The role of gut microbiota in disease management: Ayurvedic perspectives on metabolic diseases and health by Bhawana Jangra, Shreyanshi Kulshreshtha, Alok Goyal, Sanjay M. Jachak

“…Purpose: This review is aimed at collecting scientific insights on gut health, focusing on traditional medicinal plants like Babool, Turmeric, Ashwagandha, Ghrit kumari, Tulsi, Harad, Baheda, Amala, and the Triphala formulation. …”
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