Confocal Microscopy for Detection of Intracellular Bacteria in Urine Samples of patients Suspected with Urinary Tract Infection: A Cross-sectional Observational Study by Bhagyashri Patil-Takbhate, Nilam Memane, Srikanth Tripathy, Nageswari Gandham, Shahzad Mirza

“…While the culture report showed 23 (25.0%) were E. coli, 29 (31.5%) were polymicrobial flora and 29 (31.05%) were negative for culture. …”
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Multi-omics and machine learning-driven CD8+ T cell heterogeneity score for head and neck squamous cell carcinoma by Di He, Zhan Yang, Tian Zhang, Yaxian Luo, Lianjie Peng, Jiatao Yan, Tao Qiu, Jingyu Zhang, Luying Qin, Zhichao Liu, Mouyuan Sun

“…Single-cell analysis of HNSCC tissues and peripheral blood, along with spatial transcriptome analysis, revealed that OLR1 predominantly functions in macrophages, modulating the immune microenvironment of HNSCC. …”
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Dihydroquercetin attenuated Prevotella copri-caused intestinal injury by modulating gut microbiota and bile acids in weaned piglets by Long Wang, Ruizhi Hu, Siqi Ma, Xizi Yang, Jiatai Gong, Hongkun Xiang, Mingkun Shi, Xupeng Yuan, Liang Chen, Hongfu Zhang, Bie Tan, Xi He, Jianhua He, Shusong Wu

“…Moreover, DHQ increased the expression of tight junction proteins (claudin-2, occludin and tight junction protein zonula occludens protein-1 (ZO-1) and the mRNA expression of glutathione peroxidase 4 (GPX-4) in ileum (P < 0.001). Intestinal flora analysis showed that P. copri increased the relative abundance of Prevotella (P = 0.026) and Eubacterium coprostanoligenes group (P < 0.001), but decreased the relative abundance of Lachnospiraceae NK4A136 group (P < 0.001), while supplementation of DHQ reduced the relative abundance of Prevotella (P = 0.026). …”
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Multi-trace elements-enriched functional drink accelerates gastric ulcer repair via the HGF/c-Met/STAT3 pathway by Yongnan Piao, Nuoya Wang, Mingji Jin, Jianyu Piao, Mingfeng Han, Zifei Wang, Chunhua Quan, Jishan Yin, Zhonggao Gao, Wenxiang Cui, Shuangqing Wang, Xiuquan Quan

“…It also effectively regulate the abundance and diversity of intestinal flora. Finally, FD reduced the severity of gastric mucosal injury caused by invasive factors, improved the morphological structure of gastric mucosal cells, enhanced the ability of oxidative stress, and inhibited excessive inflammatory response. …”
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Dynamics of Somatic and Comorbid Mental Disorders (Distress, Anxiety, Somatisation and Depression) in Patients with Irritable Bowel Syndrome during Therapy with Alimemazine: Result... by V. T. Ivashkin, O. Z. Okhlobystina, M. V. Mayevskaya, O. S. Shifrin, Z. A. Mamieva, Yu. E. Azimova

“…Both questionnaires were offered to the patients three times: before the start of the treatment and following 2 and 4 weeks of the therapy.Results. Teraligen therapy along with the standard symptomatic treatment has shown a significant positive dynamics of the patients’ state due to the reduction of such symptoms, as pain and burning sensation in the epigastrium, postprandial fullness, early satiety, abdominal pain before defecation, abdominal distension, impaired frequency and quality of defecation. …”
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EDF1 accelerates ganglioside GD3 accumulation to boost CD52-mediated CD8+ T cell dysfunction in neuroblastoma by Di Li, Meng Li, Zhenjian Zhuo, Huiqin Guo, Weixin Zhang, Yile Xu, Hai-Yun Wang, Jiabin Liu, Huimin Xia, Huiran Lin, Jue Tang, Jing He, Lei Miao

“…Results Three subtypes were identified using nonnegative matrix factorization (NMF), among which we highlighted an apoptotic status of infiltrated CD8+ T cells, along with the highest CD52 and EDF1 expression in Cluster3 (C3) subtypes. …”
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Integrated microfluidics-based construction of anti-BTN2A2 gel droplet cell preparations for non-invasive tumor-infiltrating lymphocyte therapy by Yishen Tian, Jingxuan Li, Na Yang, Yang Zhao, Jiancao Zuo, Hang Xiong, Yiwen Pan, Li Xiao, Min Su, Feng Han, Zhixu He, Rong Hu

“…This preparation was surface modified with BTN2A2 antibodies and internally contained T cells isolated from the blood of tumor hosts, along with simulated collagen peptide CMP. Specifically, the cell preparation exerted its anti-tumor effects through multiple mechanisms: Firstly, the surface BTN2A2 antibodies effectively inhibited the proliferation of Tregs and MDSCs within the tumor microenvironment; Secondly, leveraging the T cell antigen receptors (TCRs) present in the blood T cells, which were similar to those of tumor-infiltrating lymphocytes, significantly enhanced their targeting and cytotoxic capabilities; Furthermore, the CMP component within the droplets effectively promoted the infiltration of T cells into tumor tissues. …”
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Life after prostate cancer diagnosis: protocol for a UK-wide patient-reported outcomes study by David H Brewster, Victoria Cairnduff, Eila Watson, Amy Downing, Adam W Glaser, Penny Wright, William Cross, Conan Donnelly, Anna Gavin, Carol Rivas, Richard Wagland, Peter Selby, Luke Hounsome, Therese Kearney, Rebecca Mottram, Majorie Allen, Oonagh McSorley, Hugh Butcher, Paul Kind, James W H Catto, Dyfed Huws, Emma McNair, Lauren Matheson, Johana Nayoan, Mike Horton, Jessica Corner, Julia Verne

“…To ensure detailed understanding of issues of importance, qualitative interviews will be undertaken with a sample of men who complete the survey across the UK (n=∼150) along with a small number of partners/spouses (n=∼30).Ethics and dissemination The study has received the following approvals: Newcastle and North Tyneside 1 Research Ethics Committee (15/NE/0036), Health Research Authority Confidentiality Advisory Group (15/CAG/0110), NHS Scotland Public Benefit and Privacy Panel (0516-0364), Office of Research Ethics Northern Ireland (16/NI/0073) and NHS R&amp;D approval from Wales, Scotland and Northern Ireland. …”
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Identification and Spatial Mapping of Locality of Streetscapes in Shanghai Hengfu Historic and Cultural Preservation Area by Yuhan SHAO, Sinan YIN, Dongbo MA, Yuting YIN

“…The visual diversity of streetscape at the intersection between Changshu Road and Huaihai Middle Road and along Jianguo West Road in the south is poor, and the permeability is also quite poor. …”
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Genetic characteristics and pathogenesis of clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus isolated from poultry in South Korea, 2022–2023 by Ra Mi Cha, Min-Ji Park, Yoon-Gi Baek, Yu-Na Lee, Yunyueng Jang, Yong-Myung Kang, Gyeong-Beom Heo, Se-Hee An, Kwang-Nyeong Lee, Jae-Kyeom Kim, Hye-Ryoung Kim, Youn-Jeong Lee, Eun-Kyoung Lee

“…This study aimed to investigate the genetic characteristics of H5N1 HPAIVs isolated during the 2022–2023, along with their pathogenicity and transmissibility in chickens and ducks. …”
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Diagnostics and treatment of gastroesophageal reflux disease: clinical guidelines of the Russian gastroenterological association by V. T. Ivashkin, I. V. Mayev, I. . A.s. Trukhmanov, Ye. K. Baranskaya, O. B. Dronova, O. V. Zayratyants, R. G. Sayfutdinov, A. A. Sheptulin, T. L. Lapina, S. S. Pirogov, Yu. A. Kucheryavy, O. A. Storonova, D. N. Andreyev

“…PPIs are applied for the long­term baseline treatment (no less than 4-8 wks) and maintenance therapy (6-12 months). …”
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Exploring the role of ESR1 mutations in metastatic hormone receptor-positive breast cancer T cell immune surveillance disruption by Morgane Lopez, Laurie Spehner, Fabrice André, Julien Viot, Evan Seffar, Amélie Marguier, Elsa Curtit, Guillaume Meynard, Erion Dobi, Sylvain Ladoire, Romain Boidot, Romain Loyon, Valentin Derangere, François-Clément Bidard, Christophe Borg, Laura Mansi, Marie Kroemer

“…Finally, we used Bulk RNA-Seq sequencing along with MCPcounter, a cellular deconvolution method, to investigate the immune contexture of ESR1-mutated BC. …”
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Compensatory synaptotagmin-11 expression conceals parkinson’s disease-like phenotypes in parkin knockout mice by Nan Dong, Zhenli Xie, Anqi Wei, Yuxin Yang, Yongning Deng, Xu Cheng, Bianbian Wang, Yang Chen, Yuhao Gu, Jingyu Yao, Yuhao Qin, Chaowen Zheng, Xi Zhang, Yuqing Zhang, Xinjiang Kang, Guoqing Chen, Qiumin Qu, Changhe Wang, Huadong Xu

“…Importantly, the overexpression of Syt11 alone is capable of inducing PD-like motor and non-motor impairments, as well as the impaired DA release and reuptake, which is essential for parkin-associated pathogenesis of PD. …”
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Efficacy of 5 µg and 10 µg Dexmedetomidine as Adjuvants to 3 mL 0.5% Hyperbaric Bupivacaine in Pelvic and Lower Limb Orthopaedic Surgeries: A Randomised Clinical Study by Jigisha Bharatbhai Mehta, Shreya Jatin Patel, Nilesh Vrajmohan Shah, Dinesh K Chauhan, Sara Mary Thomas

“…The onset and duration of sensory and motor blockade, as well as the duration of analgesia, were noted, along with the monitoring of haemodynamic parameters and any side effects. …”
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Influence of ethnicity on cyclin-dependent kinase inhibitor efficacy and toxicity: A systematic review and meta-analysis by Roberto Buonaiuto, Aldo Caltavituro, Margherita Tafuro, Alessandra Longobardi, Giuliana Pavone, Pierluigi De Santis, Roberta Caputo, Carmine De Angelis, Lucia Del Mastro, Fabio Puglisi, Mario Giuliano, Grazia Arpino, Martina Pagliuca, Michelino De Laurentiis

“…Conclusions: The combination of CDK4/6i and ET significantly improves PFS and OS compared to ET alone in both Asian and non-Asian patients with HR+/HER2-aBC. …”
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Influence of Exposure to Different Green Rooftop Spaces on Stress Restoration of College Students by Zhixiong ZHUO, Liang DONG

“…ObjectiveAlong with the continuous progress of urbanization, the number of people living in cities is increasing. …”
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Editorial by Lindsay B. Flynn

“…The discussion paper by Elena Lutz identifies a two-pronged strategy to address the affordability challenges that young cohorts face in the German housing market. …”
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Enterococcus and Eggerthella species are enriched in the gut microbiomes of COVID-19 cases in Uganda by Carolina Agudelo, David Patrick Kateete, Emmanuel Nasinghe, Rogers Kamulegeya, Christopher Lubega, Monica Mbabazi, Noah Baker, Kathryn Y. Lin, Chang C. Liu, Arthur Shem Kasambula, Edgar Kigozi, Kevin Komakech, John Mukisa, Kassim Mulumba, Patricia Mwachan, Brenda Sharon Nakalanda, Gloria Patricia Nalubega, Julius Nsubuga, Diana Sitenda, Henry Ssenfuka, Giana T. Cirolia, Jeshua T. Gustafson, Ruohong Wang, Moses Luutu Nsubuga, Fahim Yiga, Sarah A. Stanley, Bernard Ssentalo Bagaya, Alison Elliott, Moses Joloba, Ashley R. Wolf

“…We profiled the gut microbiomes of 83 symptomatic individuals who tested positive for SARS-CoV-2 along with 43 household contacts who did not present any symptoms of COVID-19. …”
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Editorial by Jörg Tremmel, Edward Page, Konrad Ott

“…The authors give three reasons why these nations should act alone: ability to act; responsibility to act; self-interest in acting. …”
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