Implementation of genomic surveillance of SARS-CoV-2 in the Caribbean: Lessons learned for sustainability in resource-limited settings. by Nikita S D Sahadeo, Soren Nicholls, Filipe R R Moreira, Áine O'Toole, Vernie Ramkissoon, Charles Whittaker, Verity Hill, John T McCrone, Nicholas Mohammed, Anushka Ramjag, Arianne Brown Jordan, Sarah C Hill, Risha Singh, Sue-Min Nathaniel-Girdharrie, Avery Hinds, Nuala Ramkissoon, Kris V Parag, Naresh Nandram, Roshan Parasram, Zobida Khan-Mohammed, Lisa Edghill, Lisa Indar, Aisha Andrewin, Rhonda Sealey-Thomas, Pearl McMillan, Ayoola Oyinloye, Kenneth George, Irad Potter, John Lee, David Johnson, Shawn Charles, Narine Singh, Jacquiline Bisesor-McKenzie, Hazel Laws, Sharon Belmar-George, Simone Keizer-Beache, Sharra Greenaway-Duberry, Nadia Ashwood, Jerome E Foster, Karla Georges, Rahul Naidu, Marsha Ivey, Stanley Giddings, Rajini Haraksingh, Adesh Ramsubhag, Jayaraj Jayaraman, Chinnaraja Chinnadurai, Christopher Oura, Oliver G Pybus, Joy St John, Gabriel Gonzalez-Escobar, Nuno R Faria, Christine V F Carrington

“…To overcome this constraint, the COVID-19: Infectious disease Molecular epidemiology for PAthogen Control & Tracking (COVID-19 IMPACT) project was implemented to establish rapid SARS-CoV-2 whole genome nanopore sequencing at The University of the West Indies (UWI) in Trinidad and Tobago (T&T) and provide needed SARS-CoV-2 sequencing services for T&T and other Caribbean Public Health Agency Member States (CMS). …”
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Machine learning-based prediction of antimicrobial resistance and identification of AMR-related SNPs in Mycobacterium tuberculosis by Yi Xu, Ying Mao, Xiaoting Hua, Yan Jiang, Yi Zou, Zhichao Wang, Zubi Liu, Hongrui Zhang, Lingling Lu, Yunsong Yu

“…Abstract Background Mycobacterium tuberculosis (MTB) is a human-specific pathogen that primarily infects humans, causing tuberculosis (TB). …”
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Reduction of microRNA-221 in BVDV infection enhances viral replication by targeting the ATG7-mediated autophagy pathway by Zihan Chen, Jingyu Wang, Baochun Lu, Wenxin Meng, Yufan Zhu, Qifeng Jiang, Duo Gao, Zihang Ma, Huijuan Zeng, Jinping Chen, Shizhe Liu, Zhen Wang, Kun Jia

“…MicroRNAs, a class of small noncoding RNAs, play pivotal roles in regulating a myriad of biological processes.The ATG7-LC3 pathway, a canonical autophagy mechanism, is integral in defending against pathogenic invasion and maintaining cellular homeostasis. …”
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Gene expression profiling of human umbilical vein endothelial cells overexpressing CELF2 as diagnostic targets in diabetes-induced erectile dysfunction by Daniyaer Nuerdebieke, Lizhong Yao, Lange Guo, Jiuzhi Li, Hongliang Jia, Yukui Nan

“…BackgroundErectile dysfunction (ED) is a common complication of diabetes mellitus (DM), and because of its complex neurovascular etiology, the associated molecular pathogenic mechanisms are not fully understood. This study investigated the important functions and potential molecular regulatory roles of CELF2 in DMED.MethodsAn in vitro HUVEC model with CELF2 overexpression was successfully established via transfection with a CELF2-overexpressing lentiviral vector. …”
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The influence and lag-effect of temperature and precipitation on the incidence and mortality of tuberculosis, 2000–2021: an observational study by Qiao Liu, Qiao Liu, Yaping Wang, Min Liu, Min Liu, Yanlin Zhao, Jue Liu, Jue Liu, Jue Liu, Jue Liu

“…Climate change may influence TB burden through effects on human health, living conditions, and pathogen transmission, yet its long-term impact remains underexplored.MethodsThis observational study integrated data on temperature and precipitation obtained from NCEI/NOAA, TB burden from the Global Burden of Disease Study 2021, and socio-economic covariates from the World Bank open data platform. …”
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Eco-friendly silver nanoparticles from garlic: a novel therapeutic approach for treating Escherichia fergusonii wound infections by Sozan M. Abdelkhalig, Arwa Gamal Ali, Mohamed Farouk Ghaly, Nada K. Alharbi, Maha Alharbi, Mahmoud M. Bendary, Amira I. Abousaty

“…IntroductionComplicated wound infections pose a significant challenge to patient recovery and healthcare systems, particularly due to the emergence of less common but highly resistant multidrug-resistant (MDR) pathogens that undermine the efficacy of conventional antibiotic therapies. …”
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Fructose activates a stress response shared by methylglyoxal and hydrogen peroxide in Streptococcus mutans by Alejandro R. Walker, Danniel N. Pham, Payam Noeparvar, Alexandra M. Peterson, Marissa K. Lipp, José A. Lemos, Lin Zeng

“…Here, we performed a series of analyses on the gene regulation of a dental pathogen Streptococcus mutans by exposing it to fructose and other important stress agents. …”
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Effects of the sulfated polysaccharides dextran sulfate and heparin on shrimp immunity and infection by white spot syndrome virus and Vibrio parahaemolyticus by Soraat Amphan, Pasunee Laohawutthichai, Thapanan Jatuyosporn, Premruethai Supungul, Anchalee Tassanakajon, David G. Fernig, Kuakarun Krusong

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Is there a dependence between expression of integrin receptors by peripheral blood immune cells and duration of tuberculous granuloma existence in the patients? by О. V. Berdyugina

“…This is due to high resistance of the pathogen and changing mechanisms of bacterial perception by the human immune system thus requiring closer examination of the issue. …”
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Lipoprotein N-terminal modification in Bacillus: a new paradigm for extracellular acetylation and species-dependent Toll-like receptor 2 immunomodulation by Gloria Komazin, Rachel M. Wigmore, Aditi M. Ranade, Amena A. Rizk, John H. Gardiner, Timothy C. Meredith

“…Lipoprotein chemotypes are thus critical determinants governing environmental and pathogen differentiation among Bacillus spp.…”
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Respiratory tract antimicrobial peptides more effectively killed multiple methicillin-resistant Staphylococcus aureus and nontypeable Haemophilus influenzae isolates after disrupti... by Nikola Kurbatfinski, Joseph A. Jurscisek, Kathryn Q. Wilbanks, Steven D. Goodman, Lauren O. Bakaletz

“…If we treat biofilms formed by diverse human pathogens with a targeted monoclonal antibody directed at protective domains of bacterial DNA-binding proteins integral to the structural stability of the eDNA-rich biofilm matrix, they are rapidly disrupted with concomitant release of the resident bacteria. …”
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Interleukin-27 as a Novel Biomarker for Early Cardiopulmonary Failure in Enterovirus 71-Infected Children with Central Nervous System Involvement by Mingyuan Huang, Wenjing Du, Jun Liu, Haiyang Zhang, Longbin Cao, Weiqing Yang, Hui Zhang, Zhiyong Wang, Pei Wei, Weiquan Wu, Zhulin Huang, Ying Fang, Qiling Lin, Xingwen Qin, Zhizhong Zhang, Keyuan Zhou, Jincheng Zeng

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Novel type II toxin-antitoxin systems with VapD-like proteins by Konstantin Gilep, Dmitry Bikmetov, Aleksandr Popov, Anastasiia Rusanova, Shunsuke Tagami, Svetlana Dubiley, Konstantin Severinov

“…The VapD nuclease and the VapX antitoxin comprise a type II TA system that contributes to the virulence of the human pathogen Haemophilus influenzae. We analyzed the diversity and evolution of VapD-like proteins. …”
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Clinical and functional outcomes of masquelet technique for treating Fracture-related Infections(FRIs) in shoulder girdle by Jingshu Fu, Jie Shen, Shulin Wang, Dong Sun, Xiaohua Wang, Shengpeng Yu, Zhao Xie, Hongri Wu

“…Abstract Structural and functional integrity pose a challenge for the treatment of fracture-related infections (FRI) in the shoulder girdle. …”
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Bridging viral hepatitis and liver cancer: Emerging concepts in pathogenesis and therapeutic innovation by Keyin Zheng, Aimin Jiang, Zhengrui Li, Li Chen, Kailai Li, Junyi Shen, Hank Z. H. Wong, Quan Cheng, Jian Zhang, Anqi Lin, Peng Luo

“…Results This review systematically elucidates the pathogenic mechanisms of HBV‐ and HCV‐related HCC and provides comprehensive analysis of the common molecular mechanisms underlying viral hepatitis‐to‐HCC transformation. …”
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Challenges and Potential of Remote Sensing for Assessing <i>Salmonella</i> Risk in Water Sources: Evidence from Chile by Rayana Santos Araujo Palharini, Makarena Sofia Gonzalez Reyes, Felipe Ferreira Monteiro, Lourdes Milagros Mendoza Villavicencio, Aiko D. Adell, Magaly Toro, Andrea I. Moreno-Switt, Eduardo A. Undurraga

“…Satellite sensors capture environmental proxies (e.g., vegetation cover, surface moisture, and turbidity) but not pathogens. Our goal was to identify proxies that reliably correlate with <i>Salmonella</i>. …”
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Electrochemical Biosensors 3D Printed by Fused Deposition Modeling: Actualities, Trends, and Challenges by Luiz Ricardo Guterres Silva, Carlos Eduardo Costa Lopes, Auro Atsushi Tanaka, Luiza Maria Ferreira Dantas, Iranaldo Santos Silva, Jéssica Santos Stefano

“…This review explores the integration of FDM in fabricating biosensing platforms tailored for clinical diagnostics, emphasizing its role in detecting various biomarkers and viral pathogens. …”
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Computational Identification of Most Deleterious Missense Mutations in Human PD-1 Gene by Haitham Ahmed Al-Madhagi

“…In conclusion, the comprehensive screening process undertaken in this study has successfully identified R38C, D61V, R94C, and D117V as the primary mutations contributing to genetic cancer progression and immunotherapeutic resistance against PD-1 blockers. The integration of protein topology analysis, structural alignment, docking studies with PD-L1, and assessment of protein flexibility have collectively provided robust evidence to support the pathogenic significance of these mutations.…”
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A Look into the Melting Pot: The mecC-Harboring Region Is a Recombination Hot Spot in Staphylococcus stepanovicii. by Torsten Semmler, Ewan M Harrison, Antina Lübke-Becker, Rainer G Ulrich, Lothar H Wieler, Sebastian Guenther, Ivonne Stamm, Anne-Merethe Hanssen, Mark A Holmes, Szilvia Vincze, Birgit Walther

“…<h4>Introduction</h4>Horizontal gene transfer (HGT) is an important driver for resistance- and virulence factor accumulation in pathogenic bacteria such as Staphylococcus aureus.<h4>Methods</h4>Here, we have investigated the downstream region of the bacterial chromosomal attachment site (attB) for the staphylococcal cassette chromosome mec (SCCmec) element of a commensal mecC-positive Staphylococcus stepanovicii strain (IMT28705; ODD4) with respect to genetic composition and indications of HGT. …”
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The putative effector BgtE-20069a, secreted from Blumeria graminis f. sp. tritici, suppresses plant immunity to facilitate wheat infection by Tong Yan, Gui Cheng, Yamei Zhai, Lisha Wu, Shuangjun Gong, Dazhao Yu, Lijun Yang, Fansong Zeng

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