A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae by Juan Zhou, Zirong Wu, Lei Yu, Xiaolan Huang, Fei Xiao, Yu Zhang, Linglong Wan, Jin Fu, Nan Jia, Min Chen, Chunrong Sun, Zhaomin Feng, Yi Wang

“…Klebsiella pneumoniae (K. pneumoniae) was a prevalent nosocomial pathogen and a major cause of neonatal sepsis, highlighting the critical need for simple, rapid, sensitive and reliable diagnosis for K. pneumoniae infection. …”
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First molecular detection of Mycoplasma agassizii in captive tortoises in Portugal by Manuel Louro, Rui Patrício, Rui Patrício, Rui Patrício, Rui Patrício, André Pereira, André Pereira, André Pereira, André Pereira, André Pereira, Andreia Valença, Andreia Valença, Andreia Valença, Margarida Alves, Margarida Alves, Margarida Alves

“…DNA extraction success and sample integrity were confirmed by partial amplification of the tortoise mitochondrial 12S rRNA gene in 92.9% of cases (78/84), which were then screened for M. agassizii using a species-specific PCR targeting the 16S rRNA gene.Results and discussionThe pathogen DNA was detected in 66.7% (52/78) of individuals. …”
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Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions by Solène N. Lefebvre, Mark Nijland, Ivan Maslov, Dirk J. Slotboom

“…They have been linked to pathogen virulence and are potential targets for antimicrobials. …”
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Campylobacter jejuni permeabilizes the host cell membrane by short chain lysophosphatidylethanolamines by Xuefeng Cao, Chris H.A. van de Lest, Liane Z.X. Huang, Jos P.M. van Putten, Marc M.S.M. Wösten

“…Lysophospholipids (LPLs) are crucial for regulating epithelial integrity and homeostasis in eukaryotes, however the effects of LPLs produced by bacteria on host cells is largely unknown. …”
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Comparison of Raman spectroscopy with mass spectrometry for sequence typing of Acinetobacter baumannii strains: a single-center study by Suling Liu, Ni Zhang, Jiawei Tang, Chong Chen, Weisha Wang, Jingfang Zhou, Long Ye, Xiaoli Chen, ZhengKang Li, Liang Wang

“…The objective was to assess the feasibility of this integrated method for efficient sequence typing of A. baumannii strains. …”
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Dominance of recombinant DWV genomes with changing viral landscapes as revealed in national US honey bee and varroa mite survey by Poppy J. Hesketh-Best, Dean A. Mckeown, Krisztina Christmon, Steven Cook, Anne Marie Fauvel, Nathalie A. Steinhauer, Declan C. Schroeder

“…The viromes of US commercial apiaries and their ectoparasitic mites are poorly characterized at a strain level and there is a need to integrate genomics into pathogen surveillance. We sequenced RNA viromes from 383 adult bees and 173 mites pooled samples from 11 major US beekeeping hubs in 2021, assembling 45 complete and 1702 partial genomes. …”
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Whole-genome sequencing and phylogenetic analysis of Salmonella isolated from pullets through final raw product in the processing plant of a conventional broiler complex: a longitu... by Yagya Adhikari, Matthew A. Bailey, Steven Kitchens, Pankaj Gaonkar, Luis R. Munoz, Stuart B. Price, Dianna V. Bourassa, Laura Huber, Richard J. Buhr, Kenneth S. Macklin

“…Furthermore, improper cooking or handling of contaminated raw chicken meat and meat products with Salmonella and other zoonotic pathogens can potentially cause foodborne illness in humans.…”
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The Interplay of the Gut Microbiome, Bile Acids, and Volatile Organic Compounds by Nidhi M. Sagar, Ian A. Cree, James A. Covington, Ramesh P. Arasaradnam

“…BA metabolites as a result of microbial transformations act as signaling molecules and have demonstrated regulation of intestinal homeostasis through the TGR5 and FXR receptors by inhibiting inflammation, preventing pathogen invasion, and maintaining cell integrity. …”
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NLRP3 inflammasome and pyroptosis: implications in inflammation and multisystem disorders by Xiaodi Li, Zhiyuan Zhang, Yang Han, Mianzhi Zhang

“…The NLRP3 inflammasome is a multiprotein complex that senses diverse pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs), activating the pyroptosis pathway. …”
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Persistent salmonellosis causes pancreatitis in a murine model of infection. by Kathleen E DelGiorno, Jason W Tam, Jason C Hall, Gangadaar Thotakura, Howard C Crawford, Adrianus W M van der Velden

“…Increased knowledge of how pathogenic bacteria can cause pancreatitis will provide a more integrated picture of the etiology of the disease and could lead to the development of new therapeutic approaches for treatment and prevention of pancreatitis and pancreatic ductal adenocarcinoma.…”
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Climate Change Impacts on Agriculture: A Review of Plant Diseases and Insect Pests in Ethiopia and East Africa, With Adaptation and Mitigation Strategies by Alemu Nega

“…Climate change alters the dynamics of plant diseases by affecting pathogen life cycles, enhancing survival rates, and increasing host vulnerability. …”
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Major Genes for Powdery Mildew Resistance in Research and Breeding of Barley: A Few Brief Narratives and Recommendations by Antonín Dreiseitl

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Ultrasensitive Bead-Based Immunoassay for Real-Time Continuous Sample Flow Analysis by Yuri M. Shlyapnikov, Elena A. Shlyapnikova

“…Its performance is demonstrated by simultaneous detection of model pathogen biomarkers, cholera toxin (CT) and staphylococcal enterotoxin B (SEB), with a detection limit of 0.1 fM and response time of under 10 min. …”
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Placental Inflammation in Preterm Premature Rupture of Membranes and Risk of Neurodevelopmental Disorders by Elizabeth Marie Cervantes, Sylvie Girard

“…The disruption of fetal membrane integrity introduces potential infection and inflammation into the intrauterine environment, triggering immune responses that may affect fetal development. …”
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Immuno-metabolic diseases and therapeutics: molecular mechanisms via inflammasome signaling by Joo-Hui Han

“…While inflammasome-induced pyroptosis, a form of lytic cell death, can play protective roles in pathogen clearance, excessive or dysregulated activation is more commonly associated with chronic inflammation and tissue damage. …”
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Inhibitory Effects of Garlic Extract on Hop Stunt Viroid in Micropropagated Grapevine Plantlets by Chae-Min Kang, Rae-Dong Jeong

“…Hop stunt viroid (HSVd) is a major pathogen that affects grapevine health and causes substantial economic losses in grape cultivation. …”
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Next-generation sequencing applications in food science: fundamentals and recent advances by Joel Tigrero-Vaca, Byron Díaz, Ganyu Gu, Juan Manuel Cevallos-Cevallos

“…NGS techniques, including metagenetics, metagenomics, and metatranscriptomics, enable culture-independent pathogen detection, antimicrobial resistance surveillance, and detailed microbial profiling, significantly improving food safety monitoring and outbreak prevention. …”
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Preserving Darwin’s fox: genomic tools for the conservation of South America’s most endangered canid by Cristóbal Valenzuela-Turner, José Horacio Grau, Jörns Fickel, Jörns Fickel, Daniel W. Förster

“…Here we highlight the potential of HTS to address these challenges, such as clarifying its taxonomy, demographic history, geographic distribution, population structure, genetic diversity, and pathogen exposure. Integrating molecular data into conservation planning will be pivotal in ensuring the long-term survival of Darwin’s fox by identifying priorities for targeted management interventions, highlighting areas of critical habitat for conservation, and guiding genetic rescue efforts to enhance genetic diversity and resilience.…”
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Analysis of engineered T7 bacteriophages containing genetic sequences encoding antimicrobial peptides by Tobias Ludwig, Daniela Volke, Andor Krizsan

“…Because of the global spread of multi- and pan-resistant bacteria, there is a need to identify, research, and develop new strategies to combat these pathogens. In a previous proof-of-concept study, we presented an innovative strategy by genetically modifying lytic T7 bacteriophages. …”
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Unraveling <i>Helicobacter pylori</i>: Insights into Pathogenesis, Immune Evasion, and Progress Toward Effective Vaccination by Ayman Elbehiry, Eman Marzouk, Adil Abalkhail

“…By elucidating the molecular and cellular mechanisms underlying host–pathogen interactions, we explore how these insights inform antigen selection, adjuvant optimization, and delivery strategies. …”
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