Comparative Analysis of Bacterial Conjunctivitis in the Adult and Pediatric Inpatient vs. Outpatient Population by Adela Voinescu, Corina Musuroi, Monica Licker, Delia Muntean, Silvia-Ioana Musuroi, Luminita Mirela Baditoiu, Dorina Dugaesescu, Romanita Jumanca, Mihnea Munteanu, Andrei Cosnita

“…In the case of hospitalized children, conjunctivitis was an integral part of the age-related pathology.…”
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Modeling fire‐related smoke inhalation injury using the human lung‐on‐a‐chip and organoid platform: Pathogenesis insights and therapeutic evaluation by Junmin Li, Dezhong Zhang, Yan Meng, Yongqing Chang, Wenbo Wei, Peng Wu, Lin Peng, Wei Chang, Wei Wang, Jie Huang, Jingjing Fang, Keming Zhu, Xiaojian Wan

“…Injury responses of endothelial and epithelial cells to smoke exposure were thoroughly assessed at the organ level. Integrating proteomics and molecular biology techniques, along with comparisons to animal models, identified disease‐specific pathways related to the spliceosome and carbon metabolism, as well as pathogenic molecules such as catechol‐O‐methyltransferase (COMT) and nitrilase 1 (NIT1). …”
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Preparation and Characterization of Novel Nanofibrous Composites Prepared by Electrospinning as Multifunctional Platforms for Guided Bone Regeneration Procedures by Aleksandra Sierakowska-Byczek, Julia Radwan-Pragłowska, Łukasz Janus, Tomasz Galek, Natalia Radwan-Pragłowska, Karol Łysiak, Piotr Radomski, Mirosław Tupaj

“…Guided bone regeneration (GBR) and guided tissue regeneration (GTR) techniques utilize membranes that act as scaffolds for bone and tissue growth while serving as barriers against rapidly proliferating cells and pathogens. Commonly used membranes, such as poly(tetrafluoroethylene) (PTFE) and collagen, have significant limitations—PTFE is non-bioresorbable and requires secondary removal, while collagen lacks adequate mechanical strength and exhibits unpredictable degradation rates. …”
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Transplantation of Umbilical Cord-Derived Mesenchymal Stem Cells Attenuates Surgical Wound-Induced Blood-Brain Barrier Dysfunction in Mice by Jie Yang, Hongyu Li, Mingzi Ran, Shuxu Yang, Kui Ma, Cuiping Zhang, Minglu Xiao, Yuguang Yang, Xiaobing Fu, Siming Yang

“…These findings suggest that UC-MSCs transplantation is a highly efficient and promising approach on protecting the integrity of BBB which caused by peripheral traumatic injuries.…”
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Homoplasmic m.591C >T variant in the mitochondrial MT-TF exhibits phenotypic heterogeneity: a family report by Shanshan Fan, Aiwen Wu, Feilong Wang, Jinliang Li

“…The m.591C > T variants were classified as pathogenic. Conclusions This case report highlights the significant clinical heterogeneity associated with the m.591C>T variant in the MT-TF even within the same family. …”
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Development of a semicomprehensive detection method for paramyxoviruses and its validation using Indonesian bats by Dela Ria Nesti, Kyoko Hayashida, Tatsuki Sugi, Wayan T. Artama, Hery Wijayanto, Naoko Kawai, Junya Yamagishi

“…Here, we developed a semicomprehensive detection method integrating group-wide RT-PCR for paramyxoviruses and multiplex next-generation sequencing. …”
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Laminar fluid ejection device enables high yield and preservation of mRNA and SaRNA LNP formulations by Chia Hao Ho, Irafasha C. Casmil, Manu Sharma, Tim Rees, Kenza Enright, Nick Allan, Anna K. Blakney

“…Intranasal administration offers a promising approach for respiratory vaccines, providing mucosal immunity at the primary entry site of pathogens. However, the impact of different aerosolization delivery systems on RNA-LNP stability, recovery volume and functionality is not well understood. …”
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Blue Light Therapy in Dermatological Practice: A Review by Giuseppe Lodi, Fortunato Cassalia, Mario Sannino, Giovanni Cannarozzo, Adone Baroni, Simone Amato, Elena Zappia, Giovanni Pellacani, Steven Paul Nisticò

“…We delve into the antimicrobial properties of blue light, highlighting its ability to generate reactive oxygen species that target and destroy pathogenic microorganisms such as Cutibacterium acnes. …”
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Comparative evaluation of DNA synthesis for qPCR analysis from oral squamous cell carcinoma tissues - A rapid and robust isolation technique for gene expression studies by Ramya Mahalingam, Vivek Narayanan, Magesh Karuppur Thiagarajan, T. Jayaprakash, K.V. Leela

“…Therefore, before interpreting the results of PCR-based diagnostic assays, it is imperative to confirm the DNA preparation and integrity, particularly if no pathogenic DNA is identified. …”
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Antifungal Activity of Rice Husk-Derived Liquid Smoke: Growth Suppression of Rhizoctonia solani and Bioactive Compound Profiling by Ankardiansyah Pandu Pradana, Deril Indana Damayanti, Muh Adiwena, Ahmed Ibrahim Alrashid Yousif, Diana Putri

“…R. solani was isolated from infected rice plants, and its pathogenicity was confirmed on rice seeds and seedlings. …”
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Natural products alleviate viral pneumonia by modulating inflammatory and oxidative-stress pathways by Yifu Tie, Yifu Tie, Han Liu, Tong Zhang, Tianwei Meng, Qun Liang

“…Viral pneumonia, primarily caused by influenza viruses, coronaviruses, and other respiratory pathogens, is characterized by direct alveolar epithelial injury and an excessive immune response, leading to severe inflammation, oxidative stress, and, in critical cases, acute respiratory distress syndrome and multi-organ failure. …”
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Genomic Analysis of Antibiotic Resistance and Virulence Profiles in <i>Escherichia coli</i> Linked to Sternal Bursitis in Chickens: A One Health Perspective by Jessica Ribeiro, Vanessa Silva, Catarina Freitas, Pedro Pinto, Madalena Vieira-Pinto, Rita Batista, Alexandra Nunes, João Paulo Gomes, José Eduardo Pereira, Gilberto Igrejas, Lillian Barros, Sandrina A. Heleno, Filipa S. Reis, Patrícia Poeta

“…This highlights the need for including such lesions in genomic surveillance programs and reinforces the importance of integrated One Health approaches.…”
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Diarrheal microbiota-derived extracellular vesicles drive intestinal homeostasis dysfunction via miR-125b/NF-κB-mediated macrophage polarization by Mengzhen Song, Wenjun Zhou, Jinping Fan, Chenhao Jia, Wen Xiong, Hong Wei, Shiyu Tao

“…Here, we revealed that EVs isolated from diarrheal donors’ intestinal microbiota trigger pro-inflammatory macrophage polarization and compromise intestinal epithelial barrier integrity in both conventional and pseudo-germ-free mice, demonstrating their intrinsic pathogenicity independent of viable microbes. miRNAs sequencing analysis identified miR-125b as a highly enriched miRNA in diarrheal microbiota-derived EVs, which directly targets the 3’ untranslated region of NF-κBIA, leading to its degradation and subsequent activation of the NF-κB signaling pathway. …”
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Identification of host genetic factors modulating β-lactam resistance in Escherichia coli harbouring plasmid-borne β-lactamase through transposon-sequencing by Hyunhee Kim, Travis Bell, Kihyun Lee, Jeongyun Jeong, James C. A. Bardwell, Changhan Lee

“…We applied the genes identified from the screening into Salmonella Typhimurium and Pseudomonas aeruginosa strains, both critical human pathogens with antibiotic resistance, and observed their significant impact on β-lactam resistance. …”
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Genetic Testing Enables the Diagnosis of Familial Hypercholesterolemia Underdiagnosed by Clinical Criteria: Analysis of Japanese Early-Onset Coronary Artery Disease Patients by Hiroshi Miyama, Yoshinori Katsumata, Mizuki Momoi, Genki Ichihara, Taishi Fujisawa, Jin Endo, Takashi Kawakami, Masaharu Kataoka, Shinsuke Yuasa, Motoaki Sano, Kazuki Sato, Keiichi Fukuda

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Regulation and function of alternative polyadenylation in development and differentiation by Lorenzo Gallicchio, Gonzalo H. Olivares, Cameron W. Berry, Margaret T. Fuller

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A Phase 1 randomized trial of homologous and heterologous filovirus vaccines with a late booster dose by Christina A. Rostad, Inci Yildirim, Carol Kao, Jumi Yi, Satoshi Kamidani, Etza Peters, Kathleen Stephens, Theda Gibson, Hui-Mien Hsiao, Karnail Singh, Paul Spearman, Courtney McCracken, Vivien Agbakoba, Kay M. Tomashek, Johannes B. Goll, Casey E. Gelber, Robert A. Johnson, Sujin Lee, Kristal Maner-Smith, Steven Bosinger, Eric A. Ortlund, Xuemin Chen, Larry J. Anderson, Jens Wrammert, Mehul Suthar, Nadine Rouphael, Evan J. Anderson

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CLINICAL AND LABORATORY EFFICIENCY OF TREATMENT OF WOMEN WITH PERIODONTAL DISEASES ON THE BACKGROUND OF BACTERIAL VAGINOSIS by A.D. Shulzhenko, T.O. Petrushanko, M.V. Mykytiuk

“…The frequency of detection of BV in modern women aged 18-45 is 67-89%. The main pathogens of BV are Gardnerella vaginalis and Atopobium vaginae. …”
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Enhancing Food Safety: Adapting to Microbial Responses Under Diverse Environmental Stressors by Imran Mohammad, Mohammed Sarosh Khan, Mohammad Rizwan Ansari, Mohammad Azhar Kamal, Mohammad Nadeem Bari, Mohammad Anwar

“…Results: Researchers have found that adaptive responses of pathogens to factors such as changes in temperature, disinfectants, and storage conditions can pose significant challenges to traditional food safety practices. …”
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Hub Genes PRPF19 and PPIB: Molecular Pathways and Potential Biomarkers in COPD by Zhao J, Ge X, Li H, Jing G, Ma W, Fan Y, Chen J, Zhao Z, Hou J

“…Jiale Zhao,1,2,&amp;ast; Xiahui Ge,3,&amp;ast; Hailong Li,4,&amp;ast; Genfei Jing,5,&amp;ast; Weirong Ma,2 Yuchun Fan,2 Juan Chen,2,6 Zhijun Zhao,7,8 Jia Hou2,6 1School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China; 2Department of Pulmonary and Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, Shanghai Ninth People’s Hospital, Shanghai, People’s Republic of China; 4Department of Respiratory Medicine, Ningxia Hospital of Integrated Traditional Chinese and Western Medicine, Yinchuan, Ningxia, People’s Republic of China; 5Department of Respiratory and Critical Care Medicine, Yongning County People’s Hospital, Yinchuan, Ningxia, People’s Republic of China; 6Department of Key Laboratory of Ningxia Stem Cell and Regenerative Medicine, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China; 7Clinical Laboratory Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China; 8Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, People’s Republic of China&amp;ast;These authors contributed equally to this workCorrespondence: Jia Hou, Email houj@live.com Zhijun Zhao, Email z15815z@163.comBackground: Chronic Obstructive Pulmonary Disease (COPD), a complex respiratory disorder, results from genetic and environmental factors. …”
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