Use of PB-Cre4 mice for mosaic gene deletion. by Andreas Birbach

“…This aberrant Cre activity due to Cre expression in the oocytes leads to different outcomes for maternally or paternally transmitted loxP-flanked alleles: Maternally inherited loxP-flanked alleles undergo recombination very efficiently, making female PB-Cre4 mice an efficient monoallelic "Cre deleter line". …”
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Detailed Study of Stars and Gas in a z = 8.3 Massive Merger with Extreme Dust Conditions by Anishya Harshan, Roberta Tripodi, Nicholas S. Martis, Gregor Rihtaršič, Maruša Bradač, Yoshihisa Asada, Gabe Brammer, Guillaume Desprez, Vince Estrada-Carpenter, Jasleen Matharu, Vladan Markov, Adam Muzzin, Lamiya Mowla, Gaël Noirot, Ghassan T. E. Sarrouh, Marcin Sawicki, Victoria Strait, Chris Willott

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Comparative evaluation of sequencing platforms: Pacific Biosciences, Oxford Nanopore Technologies, and Illumina for 16S rRNA-based soil microbiome profiling by Vladimir Veselovsky, Mikhail Romanov, Polina Zoruk, Andrey Larin, Vladislav Babenko, Maxim Morozov, Aleksandra Strokach, Natalia Zakharevich, Sadokatkhon Khamidova, Albina Danilova, Aleksey Vatlin, Vsevolod Pavshintsev, Feng Chenguang, Denis Tsybizov, Nikita Mitkin, Olesya Galanova, Alexander Zakharenko, Kirill Golokhvast, Kirill Golokhvast, Ksenia Klimina

“…Our results demonstrated that ONT and PacBio provided comparable bacterial diversity assessments, with PacBio showing slightly higher efficiency in detecting low-abundance taxa. Despite differences in sequencing accuracy, ONT produced results that closely matched those of PacBio, suggesting that ONT’s inherent sequencing errors do not significantly affect the interpretation of well-represented taxa. …”
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Isolation and Characterization of L-Asparaginase-Producing Bacteria from the Arabian–Persian Gulf Region: First Report on <i>Bacillus xiamenensis</i> ASP-J1-4 as a Producer and Its... by Ghofran M. Al-Harbi, Essam Kotb, Abeer A. Almiman, Mahmoud M. Berekaa, Salwa Alhamad, Nada F. Alahmady, Meneerah A. Aljafary, Nadiyah M. Alqazlan, Reem I. Alyami, Joud M. Alqarni, Ebtesam Abdullah Al-Suhaimi

“…The enzyme purified through DEAE-Sepharose displayed a molecular weight of 37 kDa based on the SDS-PAGE profile and lacked detectable L-glutaminase (L-GTNase) activity. Optimal enzyme activity was at 40 °C and pH 9.0, with stability at pH 7–9. …”
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The Correlation of Serum Adropin with Cardiovascular Risk Factors in the Experimental Rat Model of Chronic Kidney Disease and Its Implication in the Ameliorative Effect of Angioten... by Samaa Salah Abd-El-Fatah, Maha A. Fathy, Mohamed Ali Alabiad, Raja Aljafil, Mai Ahmed Gobran, Enssaf A. Ahmad, Ashwag S. Alsharidah, Mohammed Alorini, Sulaiman Mohammed Alnasser, Sara A. Awadh, Enas N. Morgan

“…Adropin in GII and GIII was negatively correlated with atherogenic index (P=0.019 and P=0.001, respectively), atherogenic co-efficient (P=0.012 and P=0.013, respectively), troponin I (P=0.021 and P=0.043, respectively), and nitric oxide (P=0.025 and P=0.038, respectively). …”
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Genome‐wide association study and BSR‐seq identify nitrate reductase–related genes in rice landraces (Oryza sativa L.) by Shuangshuang Luo, Zuyu Liao, Shilv Huang, Xiuzhong Xia, Zongqiong Zhang, Baoxuan Nong, Tongping Luo, Chenli Zhu, Can Chen, Hui Guo, Rui Feng, Yinghua Pan, Shuhui Liang, Yongcheng Li, Jianhui Liu, Yongfu Qiu, Danting Li, Xinghai Yang

“…However, the lower nitrogen use efficiency (NUE) results in an N fertilizer surplus, which causes many environmental problems. …”
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Inhibition of LPS-induced inflammation and signaling pathways in fallopian tube epithelial cells by estrogen and progesterone by Qianqian Li, Jianqi Liu, Bayaer Nashun, Sarina Bai, Xiaozhen Guo, Ling Wu, Tuya Bao

“…Western blotting was used to detect the protein expression of NF-κB p65, IκBα, p38, P-p38, Akt, and P-Akt in cells. …”
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Whole-genome sequencing identifies novel loci for keratoconus and facilitates risk stratification in a Han Chinese population by Yinghao Yao, Xingyong Li, Lan Wu, Jia Zhang, Yuanyuan Gui, Xiangyi Yu, Yang Zhou, Xuefei Li, Xinyu Liu, Shilai Xing, Gang An, Zhenlin Du, Hui Liu, Shasha Li, Xiaoguang Yu, Myopia Associated Genetics Intervention and Consortiums, Hua Chen, Jianzhong Su, Shihao Chen

“…For rare protein-truncating variants (PTVs) with minor allele frequency (MAF) < 5%, ZC3H11B emerged as the top prioritized gene, albeit failing to reach the significance threshold. We detected three common variants reaching genome-wide significance (P ≤ 5 × 10−8), all of which are novel to KC. …”
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A recombinant oncolytic Newcastle virus expressing MIP-3α promotes systemic antitumor immunity by Yan Sun, Liming Zhang, Feng-Ying Huang, Jin-Yan Wang, Shu-Zhen Dai, Ying-Ying Lin, Zhuoxuan Lu, Rong Cao, Guang-Hong Tan

“…Thus, we constructed a recombinant NDV expressing MIP-3α (NDV-MIP3α) as an in vivo DC vaccine for amplifying antitumor immunities.Methods The recombinant NDV-MIP3α was constructed by the insertion of MIP-3α cDNA between the P and M genes. Western blotting assay and ELISA were used to detect MIP-3α, HMGB1, IgG, and ATP in the supernatant and sera. …”
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Research advances on reisistance to kiwifruit bacterial canker by ZHANG Min, SUN Leiming, FU Rong, LIN Miaomiao, WANG Ran, QI Xiujuan

“…Since the first KBC was detected in Japan in 1984, it has been discovered in various countries around the world. …”
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Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication by Shahla Shahsavandi, Mohammad Majid Ebrahimi, Shahin Masoudi, Hasan Izadi

“…Growth kinetics of the virus on BHK-21/FX, BHK-21, and MDCK cells were evaluated by titrations of virus particles in each culture supernatant. Efficient multicycle viral replication was markedly detected in the cell at subsequent passages. …”
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Comparison of Paraquat Herbicide Removal from Aqueous Solutions using Nanoscale Zero-Valent Iron-Pumice/Diatomite Composites by Abbas Khodabakhshi, Fazel Mohammadi-Moghadam, Mohammad Mehdi Amin, Sara Hamati, Shakila Hayarian

“…The results showed that the optimal pH for both processes was 3.74. In optimal conditions, the efficiencies of D-nZVI and P-nZVI were 92.76% and 85.28%, respectively. …”
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Una red neuronal binaria para la identificación de mecanismos isomorfos. // A binary Neural network for identifying isomorphic mechanisms. by G. Galán Marín, J. M. del Castillo Granados

“…Some authors have<br />concluded that, in the general case, the traditional methods of detecting kinematic chain isomorphism have<br />been not found to be an efficient solution of the kinematic chain isomorphism problem, classified as NP-hard.…”
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Role of rehabilitation in patients with chronic obstructive pulmonary disease by Zeinb M.M. El Dardery, Abdelhfeez Moshrif, Ibrahim M. Shalan, Yasin A. Gala

“…Results Regarding post-treatment pulmonary function tests, there were statistically insignificant distinctions regarding pulmonary function tests P value greater than 0.05. Regarding baseline and follow-up arterial blood gases, a statistically significant difference was detected among PaO2 post, PaO2 pre, and regarding group 1A, 1B, 2A, and 2B P less than 0.05. …”
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Anti-B-cell therapy in patients with neuromyelitis optica spectrum disorders by S. V. Kotov, E. S. Novikova, A. S. Kotov

“…The annualized relapse rate at baseline and 18 months after the start of treatment was: all patients (n=27) — 0.6±0.3 and 0.07±0.27(p&lt;0.0001); AQP4-IgG+ patients (n=6) — 1.1±0.9 and 0.17±0.41 (p=0.028); MOG-IgG+ patients (n=14) — 0.4±0.3 and 0.07±0.28(p=0.001); AQP4-IgG-, MOG-IgG-patients (n=7) — 0.8±0.4 and 0.0±0.0 (p=0.018). …”
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Coexpression network analysis in abdominal and gluteal adipose tissue reveals regulatory genetic loci for metabolic syndrome and related phenotypes. by Josine L Min, George Nicholson, Ingileif Halgrimsdottir, Kristian Almstrup, Andreas Petri, Amy Barrett, Mary Travers, Nigel W Rayner, Reedik Mägi, Fredrik H Pettersson, John Broxholme, Matt J Neville, Quin F Wills, Jane Cheeseman, GIANT Consortium, MolPAGE Consortium, Maxine Allen, Chris C Holmes, Tim D Spector, Jan Fleckner, Mark I McCarthy, Fredrik Karpe, Cecilia M Lindgren, Krina T Zondervan

“…Differential eigengene network analysis of 8,256 shared probesets detected 22 shared modules with high preservation across adipose depots (D(ABD-GLU) = 0.89), seven of which were associated with MetS (FDR P<0.01). …”
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A Model for Automated Business Writing Assessment by Daniil Dmitrievich Zafievsky, Nadezhda Stanislavona Lagutina, Oksana Andreyevna Melnikova, Anatoliy Yurievich Poletaev

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Formation of the toxic furan metabolite 2-butene-1,4-dial through hemin-induced degradation of 2,4-alkadienals in fried foods by Hiroshi Kasai, Kazuaki Kawai, Koichi Fujisawa

“…Conclusions 1) BDA is efficiently generated from ADE in the presence of hemin under gastric conditions, and 2) BDA-derived CPL can also form under physiological conditions (pH 7.4) through the interaction of ADE, hemin, Cys, and Lys. …”
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A promising Ag2S/poly-2-amino-1-mercaptobenzene open-top spherical core–shell nanocomposite for optoelectronic devices: A one-pot technique by Alkallas Fatemah H., Rabia Mohamed, Trabelsi Amira Ben Gouider, Alrebdi Tahani A., Elsayed Asmaa M.

“…Herein, we have introduced a novel and promising optoelectronic device, the Ag2S/poly-2-amino-1-mercaptobenzene open-top spherical core–shell nanocomposite (Ag2S/P2AMB CS-nanocomposite), fabricated using cost-effective and scalable production techniques. …”
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Innovative Approaches to Forest Fire Prevention: Integrating Technology and Ecological Strategies. A Comprehensive Review by Syed Shaheer Hassan, Guang Yang, Sana Tahir, Haseeb Ahmad, Muhammad Sabir, Laila Noor, Khuzin Dinislam

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