Development of a procedure for isolation, identification and quality assessment of bovine spermatids and evaluation of their fertilizing ability in vitro by Rolando Pasquariello, Francesca Di Filippo, Sai Kamal Nag Bonumallu, Fernanda Fagali Franchi, Ramona Pistucci, Federica Franciosi, Valentina Lodde, Alessandra Iannuzzi, Alberto Maria Luciano, Tiziana A. L. Brevini, Fulvio Gandolfi

“…First, we optimized a protocol for spermatid isolation from bull testis using a discontinuous Percoll gradient and 10 μm mesh cell strainers. …”
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The clinical impact of 16S ribosomal RNA PCR and sequencing in the identification of bacterial infections: a 7-year report from a Lebanese tertiary care center by Nour Youssef, Nour Youssef, Celina F. Boutros, Fatima Dakroub, Fata Akl, Lina Reslan, Marc Finianos, Mohammad Bahij M. Moumneh, Tarek Bou Dargham, Zeinab El Zein, Zeinab El Zein, Zeinab El Zein, Amani Haddara, Rawan Korman, Sarah Khafaja, Sarah Khafaja, Sarah Khafaja, Ghassan Matar, Ghassan Matar, George F. Araj, George F. Araj, Ghassan S. Dbaibo, Ghassan S. Dbaibo, Ghassan S. Dbaibo

“…IntroductionThe identification of bacterial pathogens in the clinical setting is essential for providing optimal care and improving outcomes. The primary objective of this study was to assess the performance of the 16S test in bacterial identification from different samples and determine its impact on clinical outcomes.MethodsThis was a retrospective study of patient samples collected from all age-groups at the American University of Beirut Medical Center (AUBMC), from May 2016 to December 2022. …”
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Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer by Anne Meunier, Javier Alejandro Hernández-Castro, Nicholas Chahley, Laudine Communal, Sara Kheireddine, Newsha Koushki, Nadia Davoudvandi, Sara Al Habyan, Benjamin Péant, Anthoula Lazaris, Andy Ng, Teodor Veres, Luke McCaffrey, Diane Provencher, Peter Metrakos, Anne-Marie Mes-Masson, David Juncker

“…We detect cCTCs in every patient, with between 2–100+ cells. …”
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Extracellular vesicle digital scoring assay for assessment of treatment responses in hepatocellular carcinoma patients by Chen Zhao, Yi-Te Lee, Andrew Melehy, Minhyung Kim, Jacqueline Ziqian Yang, Ceng Zhang, Jina Kim, Ryan Y. Zhang, Junseok Lee, Hyoyong Kim, Yong Ju, Yuan-Jen Tsai, Xianghong Jasmine Zhou, Steven-Huy B. Han, Saeed Sadeghi, Richard S. Finn, Sammy Saab, David S. Lu, Jason Chiang, Jae-Ho Park, Todd V. Brennan, Steven A. Wisel, Manaf Alsudaney, Alexander Kuo, Walid S. Ayoub, Hyunseok Kim, Hirsh D. Trivedi, Yun Wang, Aarshi Vipani, Irene K. Kim, Tsuyoshi Todo, Justin A. Steggerda, Georgios Voidonikolas, Kambiz Kosari, Nicholas N. Nissen, Rola Saouaf, Amit G. Singal, Myung Shin Sim, David A. Elashoff, Sungyong You, Vatche G. Agopian, Ju Dong Yang, Hsian-Rong Tseng, Yazhen Zhu

“…Results HCC EV TR Scores, calculated from pre- and post-treatment plasma samples in the phase-2 case–control study, accurately differentiated post-treatment viable from nonviable HCC in the training (area under the ROC curve [AUROC] of 0.90, n = 49) and validation set (AUROC of 0.88, n = 51). At an optimal cutoff of 0.76 identified in the training set, HCC EV TR Score had high accuracy in detecting viable tumors (sensitivity: 76.5%, specificity: 88.2%) and found residual disease not initially observed on MRI in six patients with a median lead time of 63 days. …”
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Determination of Migration of Six Metal Ions in Food Contact Plastic by Guang-yao LING, Xiao-ting XIONG, Chun-sheng LIU, Yi-guang CHEN, Fen-wan JIANG, Xu-qian QIU, Hui-yong LI, Xiao-gang HUANG, Guo-shan HE

“…Migration solutions from 24 batches of food contact plastic were tested, while 4 of them contain detectable Cu2+, Mn2+ and Zn2+. The proposed method is easily appliable to determine metal ions in high proportion ethanol aqueous solution without needing to remove the ethanol. …”
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Estimation of Wind Turbine Blade Icing Volume Based on Binocular Vision by Fangzheng Wei, Zhiyong Guo, Qiaoli Han, Wenkai Qi

“…Icing on wind turbine blades in cold and humid weather has become a detrimental factor limiting their efficient operation, and traditional methods for detecting blade icing have various limitations. …”
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Running Parameter Analysis in 400 m Sprint Using Real-Time Kinematic Global Navigation Satellite Systems by Keisuke Onodera, Naoto Miyamoto, Kiyoshi Hirose, Akiko Kondo, Wako Kajiwara, Hiroshi Nakano, Shunya Uda, Masaki Takeda

“…Method 2 also exhibited improved performance in curved sections and detected stride asymmetries with higher consistency than Method 1. …”
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Classification of Bitter gourd leaf disease using deep learning architecture ResNet50 by Artika Artika, Wikky Fawwaz Al Maki

“…This research contributes to the development of methods for detecting bitter melon leaf disease using computer vision and machine learning, achieving high accuracy and supporting automatic disease diagnosis. …”
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Enhancement effects of seaweed iodine fertilizer application on the iodine contents of rice, vegetables and fruits in the field by HU Chunqin, LI Rui, HONG Chunlai, CAO Wenting, LIU Jiawei, ZHOU Jun, WENG Huanxin

“…The treatments of exogenous iodine were as follows：0, 0.375, 0.750, 1.500, 3.000, and 4.500 kg/hm². Spectrophotometric method was used to detect the iodine contents of crops and soil samples, and one-way analysis of variance was applied to analyze the difference of iodine contents among all treatments.The results suggested that the iodine content in the edible parts of all the tested crops were significantly enhanced (P<0.05) after biofortification by the seaweed iodine fertilizer, including rice, loofah, cucumber, tomato, eggplant, watermelon, edamame, cowpea, etc. …”
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Orchard: Building large cancer phylogenies using stochastic combinatorial search. by Ethan Kulman, Rui Kuang, Quaid Morris

“…., cancer phylogenies, offer valuable insights about cancer development and guide treatment strategies. Many methods exist that reconstruct cancer phylogenies using point mutations detected with bulk DNA sequencing. …”
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Early cerebrospinal fluid elevations of pTau-217 in severe traumatic brain injury subjects by Hamad Yadikar, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Claudia Robertson, Spyridoula Tsetsou, John B. Williamson, John B. Williamson, Damon G. Lamb, Damon G. Lamb, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Todd Kibaugh, Shih-Han Kao, Zhifeng Kou, Zhifeng Kou, Robert D. Welch, Jose-Miguel Yamal, Luis Leon-Novelo, Richard Rubenstein, Richard Rubenstein, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang

“…This study investigated whether cerebrospinal fluid (CSF) phosphorylated Tau at threonine-217 (pTau-217) could serve as an early biomarker for severe TBI (sTBI).MethodsCSF samples from 26 sTBI patients, collected between 6 and 240 h post-injury, and 19 healthy controls were analyzed using an optimized direct enzyme-linked immunosorbent assay (ELISA; sensitivity <4.7 pg/mL) for pTau-217 detection, complemented by Western blot validation. …”
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Fe-Cu-loaded γ-Al2O3 as an efficient heterogeneous Fenton catalyst for the advanced treatment of petrochemical wastewater by Shiqi Jiang, Changyong Wu, Qi Huang, Yin Yu, Peijian Zhang, Chenjie Wang, Xiyan Ou, Min Xu

“…The catalyst was synthesized via impregnation and calcination, with preparation parameters optimized using a one-factor-at-a-time method. Under optimal conditions (Fe: 0.28 mol/L; Cu: 0.20 mol/L; 500 °C; 7 h), 50.15% TOC removal was achieved using 20 g catalyst and 13.73 mmol/L H2O2. …”
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LPS2336, a New TREK-1 Channel Activator Identified by High Throughput Screening by Romane Boyer, Romane Bony, Maxence Maugis, Julien Schopp, Marion Leroux, Clément Michelin, Laurie Gonthier, Quentin Grzeskiewicz, Alexandre Jouannet, Youssef Aissouni, Bruno Didier, Mihaela Gulea, Nicolas Girard, Jean-Christophe Cintrat, Antoine Dumeige, Jérôme Busserolles, Sylvie Ducki, Stéphane Lolignier

“…The development of new pharmacological tools to study these channels and enrich our knowledge of structure–activity relationships is therefore important. We optimized a high throughput screening method based on thallium flux monitoring for the detection of TREK-1 activators in chemical libraries. …”
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Inspection of railway catenary systems using machine learning with domain knowledge integration by Kacper Marciniak, Paweł Majewski, Jacek Reiner

“…Proposed methods substantially improve metrics such as AP50, precision, recall, and F1-score, particularly in detecting small and hard-to-spot catenary components such as insulators. …”
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Facial Expression Recognition and Digital Images Infosecurity for Prevention Care System Application in Sudden Infant Death Syndrome Monitoring by Chih-Te Tsai, Chia-Hung Lin, Hsiang-Yueh Lai, Yu-En Cheng, Ping-Tzan Huang, Neng-Sheng Pai, Chien-Ming Li

“…For FER testing, the ten-fold cross-validation method was applied to evaluate the classifier’s performances, and the feasibility of the proposed method could be evaluated, achieving average Precision (%) of 91.40%, average Recall (%) of 91.15%, and average Accuracy (%) of 95.10%, respectively. …”
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Therapeutic and Diagnostic Tactics for Metastases to the Lymph Nodes of the Neck without an Identified Primary Focus by M. I. Sokolova, V. I. Pavlova

“…Of the special treatment methods, complex and combined treatment prevailed — 55 % (abs. 26). …”
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Recirculation in Veno-Venous Extracorporeal Membrane Oxygenation by Veronica Gagliardi, Giuseppe Gagliardi

“…As recirculation may significantly reduce extracorporeal oxygen delivery, leading to refractory hypoxemia, detecting and quantifying the recirculation fraction is fundamental in order to optimize VV-ECMO lung support. …”
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Cloning, expression of carboxylesterase BioH and improvement of its hydrolysis activity by directed evolution by XU Hongna, YU Hongwei

“…It shows great significance to get the protein and optimize its functions.In this study, BioH gene from E. coli K12 was cloned into pET30a (+) using double digestion method by Bam HI and Hind Ⅲ and was overexpressed in E. coli BL21 (DE3) induced by isopropyl-β-D-thiogalactoside (IPTG). …”
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Improving consistency in methane emission quantification from the natural gas distribution systems across measurement devices by J. Tettenborn, D. Zavala-Araiza, D. Zavala-Araiza, D. Stroeken, H. Maazallahi, H. Maazallahi, C. van der Veen, A. Hensen, I. Velzeboer, P. van den Bulk, F. Vogel, L. Gillespie, L. Gillespie, S. Ars, J. France, J. France, D. Lowry, R. Fisher, T. Röckmann

“…Currently, the most common method for emission quantification uses the maximum methane enhancement detected while crossing a methane plume. …”
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The concentration of single-stranded DNA-binding proteins is a critical factor in recombinase polymerase amplification (RPA), as revealed by insights from an open-source system by Francisco Cordoba-Andrade, Antolin Peralta-Castro, Paola L. García-Medel, Eduardo Castro-Torres, Rogelio Gonzalez-Gonzalez, Atzimba Y. Castro-Lara, Josue D. Mora Garduño, Claudia D. Raygoza, Noe Baruch-Torres, Alejandro Peñafiel-Ayala, Corina Diaz-Quezada, Cesar S. Cardona-Felix, Fernando Guzman Chavez, Carlos H. Trasviña-Arenas, Rogerio R. Sotelo-Mundo, Beatriz Xoconostle-Cazares, Agustino Martínez-Antonio, Luis Gabriel Brieba de Castro

“…Notably, gp32 concentration and buffer composition emerged as critical factors in optimizing RPA performance. Using this tailored system, we demonstrated successful detection of the SARS-CoV-2 N gene on lateral flow devices (LFDs) with cDNA from eight clinical samples, achieving results consistent with RT-PCR. …”
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