Simultaneous Quantification of Methotrexate and Its Metabolite 7-Hydroxy-Methotrexate in Human Plasma for Therapeutic Drug Monitoring by Xinxin Ren, Zhipeng Wang, Yunlei Yun, Guangyi Meng, Xialan Zhang, Huamin Ding, Ying Xu, Hansheng Bai, Jing Liu, Xia Li, Shouhong Gao, Lifeng Huang, Wansheng Chen

“…The pretreatment procedure was optimized with dilution after one-step protein precipitation. …”
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The Determination of Eight Biogenic Amines Using MSPE-UHPLC-MS/MS and Their Application in Regard to Changes in These Biogenic Amines in Traditional Chinese Dish-Pickled Swimming C... by Peipei Li, Yu Chen, Junlu Bai, Huicheng Yang, Pengfei He, Junjie Zeng

“…The experimental variables involved in the MSPE, including the solution pH, adsorption and desorption time, adsorbent usage, and type and volume of the eluent, were investigated and optimized. Method validation indicated that the developed method showed good linearity (R² > 0.995); the average recovery rates were 84.7% to 115%, with the intra-day and inter-day relative standard deviations (RSD, <i>n</i> = 6) ranging from 3.7% to 7.5% and 4.2% to 7.7%, respectively. …”
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Comparison of enzymatic and bisulfite conversion of circulating cell-free tumor DNA for DNA methylation analyses by Stine H. Kresse, Evy Marie Thorkildsen, Sara Brandt-Winge, Heidi Pharo, Hege Marie Vedeld, Guro E. Lind

“…Abstract Background Detection of DNA methylation biomarkers in circulating cell-free DNA (cfDNA) has great clinical potential for cancer management, but there is a high need for method optimization and standardization. …”
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Measurement Techniques for Highly Dynamic and Weak Space Targets Using Event Cameras by Haonan Liu, Ting Sun, Ye Tian, Siyao Wu, Fei Xing, Haijun Wang, Xi Wang, Zongyu Zhang, Kang Yang, Guoteng Ren

“…In contrast, event cameras—drawing inspiration from biological vision—can capture brightness changes at ultrahigh speeds and output a series of asynchronous events, thereby demonstrating enormous potential for space detection applications. Based on this, this paper proposes an event data extraction method for weak, high-dynamic space targets to enhance the performance of event cameras in detecting space targets under high-dynamic maneuvers. …”
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Enhancing wheat flour origin traceability by using laser-induced breakdown spectroscopy and Raman spectroscopy by Minghui Gu, Chao Liu, Hansong Huang, Xin Zhang, Jiguo Li, Qingbin Jiao, Liang Xu, Mingyu Yang, Xin Tan

“…The results showed that the proposed method can enhance the ability of origin traceability, and high-level fusion strategies perform the best, achieving an average accuracy of 98%. …”
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Enhancing Consumer Insights Through Multimodal Artificial Intelligence and Affective Computing by Ines Cesar, Ivo Pereira, Fatima Rodrigues, Vera L. Migueis, Susana Nicola, Ana Madureira, Jose Luis Reis, Jose Paulo Marques Dos Santos, Duarte Coelho, Daniel Alves De Oliveira

“…Multimodality and Affective Computing combined a series of robust optimizations for this challenge, implying the complexity of each application. …”
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Rapid differentiation of patients with lung cancers from benign lung nodule based on dried serum Fourier-transform infrared spectroscopy combined with machine learning algorithms by Huanyu Li, Lixue Dai, Shaomei Guo, Hongluan Wang, Lei Lei, Jie Yu, Xiaoyun Li, Jun Wang

“…While low-dose computed tomography (LDCT) screening enables earlier detection, its high false-positive rate, primarily due to benign lung nodules (BLN), necessitates more accurate diagnostic tools. …”
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Role of High Serum Tenascin C Levels as Potential Biomarker of Persistent Inflammation in Patients with Ankylosing Spondylitis Despite Treatment with cs-DMARDS or Anti-TNF Agents by Alejandro Martelli-Garcia, Yussef Esparza-Guerrero, Heriberto Jacobo-Cuevas, Ana Miriam Saldaña-Cruz, Norma Guadalupe Gonzalez-Montoya, Cesar Arturo Nava-Valdivia, Eli Efrain Gomez-Ramirez, Maria Luisa Vazquez-Villegas, Juan Manuel Ponce-Guarneros, Melissa Ramirez-Villafaña, Norma Alejandra Rodriguez-Jimenez, Alberto Daniel Rocha-Muñoz, Ernesto German Cardona-Muñoz, Jaime Morales-Romero, Laura Gonzalez-Lopez, Jorge Ivan Gamez-Nava

“…The ROC curve for serum tenascin C in active AS patients had an area under the curve = 0.78 (CI 95%: 0.65–0.91) with optimal serum tenascin C cutoff (>13.85 ng/mL). Sensitivity for detecting active AS was higher with TNC compared to ESR and CRP. …”
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Tailoring Photophysical Processes of Er3+‐Doped Gd3Ga5O12 Garnets for Enhanced Photoluminescence via Al3+ Ion Preference Substitution by Zhengjie Tian, Haiyan Wang, Yunfeng Lou, Qihang Deng, Zhi Yang, Xiyong Chen, Bingsuo Zou, Mingjian Yuan, Ruosheng Zeng

“…ABSTRACT Rare‐earth ion‐doped garnets with excellent luminescent properties show great potential for temperature sensing, displays, and nondestructive detection. However, their limited luminescent modes and low photoluminescence quantum yields (PLQY) restrict further applications. …”
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A humanized anti-MSLN×4-1BB bispecific antibody exhibits potent antitumour activity through 4-1BB signaling activation and fc function without systemic toxicity by Da-yan Zhang, Zhi-hua Zhang, Wen-ting Liu, Wei-ming Zhou, Peng-fei Zhou, Juan-juan Wei, Xue-jing Dai, Xiao-li Zeng, Yu-qiong Zhou, Han-wang Li, Heng Zhang, Ao-lin Shen, Lian-sheng Cheng, Guo-dong Shen, Yi-fu He

“…Therefore, a safe and efficient immunostimulatory molecule is urgently needed for cancer immunotherapy. Methods A novel anti-MSLN×4-1BB bispecific antibody (bsAb) was generated via antibody engineering, and its affinity and activity were detected via enzyme-linked immunosorbent assay (ELISA), flow cytometry, and T-cell activation and luciferase reporter assays. …”
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Balancing the benefits and risks of colchicine use among patients with atherosclerotic cardiovascular disease: an umbrella review of meta-analyses of randomised controlled trialsRe... by Rui-Han Bao, Na Zhao, Xiao-Feng Jiang, Zi-Han Cong, Jun Liu, Long-Dan Kang, Yu-Han Chen, Jia-Nan Sun, Wei-Yi Xing, Jia-Xin Liu, Xue-Li Bai, Hai-Chen Lv, Jing-Ying Zhao, Qi-Peng Ma, Ting-Ting Gong, Qi-Jun Wu

“…Publication bias was detected via funnel plot asymmetry or Egger’s test. …”
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Electronic Sensor-Based Automated Irrigation System for Rice Cultivated Under Alternate Wetting and Drying Technique by Mukesh Kumar, Ramesh Kumar Sahni, Abhishek M. Waghaye, Manoj Kumar, Ravindra D. Randhe

“…In this method, the rice field is initially flooded to a maximum depth of 5 cm. …”
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Long and short term fault prediction using the VToMe-BiGRU algorithm for electric drive systems by Lihui Zheng, Xu Fan, Zongshan Kang, Xinjun Jin, Wenchao Zheng, Xiaofen Fang

“…Specifically, the VToMe algorithm achieves stable detection of medium to long term system faults, while the BiGRU network achieves rapid fault prediction in the short term. …”
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Research progress and thinking on specialized drilling technology in underground coal mine by Jianlin LIU, Fei LIU, Quanxin LI, Jun FANG, Guiqin LIU, Siyi WANG, Zhiwei CHU, Jianguo ZHAO, Weifeng YANG

“…The typical application scenarios of specialized drilling technology are summarized, such as precise gas control in soft-fragmented coal seam, replacing tunnel with borehole for relieved gas drainage in roof, mine water detection and release, and grouting reinforcement of water-resisting layer.It delves into the key technical challenges encountered during implementation.Focusing on drilling equipment upgrade, innovative drilling method, and drilling parameter optimization, the study comprehensively reviews the current development status, critical technological advancements, and practical outcomes.The specialized drilling technology suitable for soft-fragmented coal seam, hard rock strata, and fractured and water sensitive strata are outlined, and the existing technical bottlenecks and challenges are identified.Given the evolving trends of diversified demand, equipment intelligence, and region-specific adaptation in underground specialized drilling technology, future research priorities are proposed: ① To enhance air directional drilling technology for soft-fragmented coal seam to improve formation adaptability and process compatibility. ② To advance high-efficiency drilling acceleration technology and tool for hard rock strata to boost rock-breaking efficiency and service life of impact drilling tool. ③ To overcome theoretical and technical barrier in pressure-controlled drilling under near-horizontal condition to strengthen borehole formation capability in fractured and water-sensitive strata of roof or floor. ④ To accelerate the improvement of the reliability and stability of intelligent drilling technology and equipment in underground coal mine, and promote the realization of the transformation of specialized drilling technology from equipment intelligence to engineering intelligence. ⑤ To research and constructe specialized drilling technology system of regional, mining area and mine, and promote their wide application and in-depth development in the coal mining industry.The study can provide an important reference for the technological innovation and efficiency improvement of drilling process in underground coal mine.…”
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Enhanced Percolation Effect in Sub‐100 Nm Nanograting Structure for High‐Performance Bending Insensitive Flexible Pressure Sensor by Jae‐Soon Yang, Min‐Ho Seo, Min‐Seung Jo, Kwang‐Wook Choi, Jae‐Shin Lee, Myung‐Kun Chung, Bon‐Jae Koo, Jae‐Young Yoo, Jun‐Bo Yoon

“…By leveraging the percolation effect within a sub‐100 nm nanograting structure, the design of the pressure sensor is optimized through numerical analysis and finite element method (FEM) simulations. …”
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Metabolic profiles and prediction of failure to thrive of citrin deficiency with normal liver function based on metabolomics and machine learning by Peiyao Wang, Duo Zhou, Lingwei Hu, Pingping Ge, Ziyan Cen, Zhenzhen Hu, Qimin He, Kejun Zhou, Benqing Wu, Xinwen Huang

“…The predictive model based on kynurenine, arginine, alanine, and aspartate provides robust diagnostic performance for detecting FTT in post-NICCD patients.…”
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Use of uterine electromyography in the prediction of preterm birth after transvaginal cervical cerclage by Haitian Xie, Menglan Zhu, Kewen Deng, Jinling Yi, Liqiong Zhu, Jianping Tan, Xiaohui Ji, Phei Er Saw, Chunwei Cao, Nengyong Ouyang, Hui Chen

“…Based on multivariate logistic regression analysis, PTB after cerclage was significantly associated with previous PTB history or mid-trimester pregnancy loss (OR: 2.87, 95%CI: 1.49–5.54) and contraction frequency detected by uEMG (OR: 2.24, 95%CI: 1.44–3.49). The AUC of contraction frequency (0.766, P<0.001) was observed, and the optimal cut-off value suggested by Youden Index was 1.75 times per hour. …”
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The acceptability of blood spot screening and genome sequencing in newborn screening: a systematic review examining evidence and frameworks by Duncan Chambers, Susan Baxter, Anastasios Bastounis, Katherine Jones, Burak Kundakci, Anna Cantrell, Andrew Booth

“…Background Population-wide newborn blood spot screening programmes are a successful public health intervention used to detect whether the baby is at risk of certain rare conditions, with the aim of earlier diagnosis and provision of optimal care and treatment. …”
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Determining Planetary Boundary Layer Height by Micro-pulse Lidar with Validation by UAV Measurements by Yueh-Chen Wang, Sheng-Hsiang Wang, Jasper R. Lewis, Shuenn-Chin Chang, Stephen M. Griffith

“…Furthermore, the Haar wavelet and the Hybrid image processing can detect the PBL development comparably well, but both methods are dependent on their initial conditions and optimized algorithm settings. …”
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In-House Validation of Four Duplex Droplet Digital PCR Assays to Quantify GM Soybean Events by Daniela Verginelli, Sara Ciuffa, Katia Spinella, Davide La Rocca, Marisa Misto, Cinzia Quarchioni, Pamela Bonini, Cristiana Fusco, Lorella Peroni, Stefania Peddis, Ugo Marchesi

“…Since the ddPCR methods provide accurate quantification, show less sensitivity to PCR inhibitors and are more suitable for multiplexing compared to the real-time PCR, the optimization of the existing assays was performed with the exception of MON87701, according to the JRC Guidance documents and technical reports. …”
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