Harmonization trial on ESR1 testing strategies in ER+/HER2- breast cancer patients: an Italian experience by Francesco Pepe, Gianluca Russo, Konstantinos Venetis, Claudia Scimone, Lucia Palumbo, Mariantonia Nacchio, Domenica di Giovanni, Claudia Sarracino, Ilaria Tomaiuolo, Elisabetta Zulato, Matteo Fassan, Daniela Righi, Giuseppe Perrone, Dario de Biase, Gabriele Casati, Fabio Pagni, Lucia Gullotti, Antonio Rizzo, Alessandro Perez, Antonio Russo, Katia Zavaglia, Gianluca Gragnano, Domenico Cozzolino, Sabrina Alfano, Cristian Scatena, Sara D’andrea, Monica Schiappacassi, Giuseppina Roscigno, Alessia Paganotti, Renzo Boldorini, Francesco Esposito, Pierlorenzo Pallante, Daniela Furlan, Marta Sbaraglia, Angelo Paolo Dei Tos, Giancarlo Troncone, Nicola Fusco, Umberto Malapelle

“…Here, we sought to harmonize technical procedures comparing technical performance of diagnostically available testing strategies on a series of three reference specimens (sample A, B, C) harboring ESR1 p.D538G mutation at different mutant allele fraction (MAF) (5.0 %, 1.0 %, 0.5 %) shared with n = 10 Italian referral institutions. Methods: A total of 10 μl of gDNA from each reference sample built to mimic clinically detectable ESR1 molecular alteration (5.0 %, 1.0 %, 0.5 % VAF) was shipped by coordinator institution to each participating group to test p.D538G ESR1 alteration leveraging own routinely available testing strategy. …”
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Identifying subgroup of severe community-acquired pneumonia based on clinical metagenomics, a multicenter retrospective cohort study by Mingqiang Wang, Mingqiang Wang, Yue Jin, Yue Jin, Wenxiao Zhang, Wenxiao Zhang, Ling Ye, Ling Ye, Huanzhang Shao, Huanzhang Shao

“…We hypothesize that subtyping sCAP based on simple inflammatory markers, organ dysfunction, and clinical metagenomics results is feasible.MethodIn this study, we retrospectively enrolled immunocompetent sCAP patients requiring invasive mechanical ventilation, who underwent clinical metagenomics from 17 medical centers. …”
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Digital augmentation of aftercare for patients with anorexia nervosa: the TRIANGLE RCT and economic evaluation by Janet Treasure, Katie Rowlands, Valentina Cardi, Suman Ambwani, David McDaid, Jodie Lord, Danielle Clark Bryan, Pamela Macdonald, Eva Bonin, Ulrike Schmidt, Jon Arcelus, Amy Harrison, Sabine Landau

“…Feedback from patients and carers The process evaluation used a mixed-methods approach to obtain participant feedback about optimising transition support and to understand factors influencing engagement with ECHOMANTRA intervention. …”
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Phenotypic and Genotypic Characterization of ESBL-, AmpC-, and Carbapenemase-Producing <i>Klebsiella pneumoniae</i> and High-Risk <i>Escherichia coli</i> CC131, with the First Repo... by Hajer Ziadi, Fadela Chougrani, Abderrahim Cheriguene, Leticia Carballeira, Vanesa García, Azucena Mora

“…Fluoroquinolone resistance (FQR) was detected in 94.1% of <i>K. pneumoniae</i> isolates. …”
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Mechanism of electroacupuncture on rats with primary dysmenorrhea based on microRNA expression spectrum and PI3K/Akt/mTOR signaling pathway by Si-an PAN, Shao-hua WANG, Han-yu YUAN, Juan LI, Xiao XUE, Zeng-hui YUE, Yu LIU

“…After the 10-day intervention, samples were collected and transmission electron microscopy was used to observe the ultrastructural changes of the cells in uterine tissue in each group. With RNA-seq method, the changes of miRNA expression spectrum in rat uterine tissue were detected. …”
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Cervical ripening at home or in hospital during induction of labour: the CHOICE prospective cohort study, process evaluation and economic analysis by Mairead Black, Cassandra Yuill, Mairi Harkness, Sayem Ahmed, Linda Williams, Kathleen A Boyd, Maggie Reid, Amar Bhide, Neelam Heera, Jane Huddleston, Neena Modi, John Norrie, Dharmintra Pasupathy, Julia Sanders, Gordon C S Smith, Rosemary Townsend, Helen Cheyne, Christine McCourt, Sarah Stock

“…Impact of COVID-19 Of responding sites, 23% reported a change in the method used for cervical ripening and 28% reported a change in criteria for offering home cervical ripening. …”
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Investigating the relationship between the Khami reservoir horizon in the Chilingar and Garangan oilfields in the Southern Dezful Embayment using surface geochemistry by Bahram Alizadeh, Arman Salehipour Bavarsad

“…The findings aim to guide efficient production management, reduce drilling risks, and advance field development strategies at lower exploration costs.Materials & MethodsThis study utilized direct geochemical techniques, including acid extraction and fluorescence analysis, to detect subsurface hydrocarbon reservoirs. …”
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Expert consensus on the evaluation and management of high-risk indeterminate pulmonary nodules by Yang Dawei, Stephan Lam, Kai Wang, Zhou Jian, Zhang Xiaoju, Wang Qi, Zhou Chengzhi, Zhang Lichuan, Bai Li, Wang Yuehong, Li Ming, Sun Jiayuan, Li Yang, Fengming Kong, Haiquan Chen, Ming Fan, Xuan Jianwei, Fred R. Hirsch, Charles A. Powell, Bai Chunxue

“…Background: The most effective method for improving the prognosis of lung cancer is the application of low-dose computed tomography (LDCT) for pulmonary nodule screening in populations at high risk. …”
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Application of a high-resolution melt assay for monitoring SARS-CoV-2 variants in Burkina Faso and Kenya by Caitlin Greenland-Bews, Sonal Shah, Morine Achieng, Emilie S. Badoum, Yaya Bah, Hellen C. Barsosio, Helena Brazal-Monzó, Jennifer Canizales, Anna Drabko, Alice J. Fraser, Luke Hannan, Sheikh Jarju, Jean-Moise Kaboré, Mariama A. Kujabi, Cristina Leggio, Maia Lesosky, Jarra Manneh, Tegwen Marlais, Julian Matthewman, Issa Nebié, Eric Onyango, Alphonse Ouedraogo, Kephas Otieno, Samuel S. Serme, Sodiomon Sirima, Ben Soulama, Brian Tangara, Alfred Tiono, William Wu, Emily R. Adams, Abdul Karim Sesay, Chris Drakeley, Feiko O. ter Kuile, Issiaka Soulama, Simon Kariuki, David J. Allen, Thomas Edwards

“…Extracted RNA from SARS-CoV-2-positive samples collected from 205 participants (112 in Burkina Faso, 93 in Kenya) enrolled in the MALCOV study (Malaria as a Risk Factor for COVID-19) between February 2021 and February 2022 were analyzed using our optimized HRM assays. With next-generation sequencing on Oxford Nanopore MinION as a reference, two HRM assays, HRM-VOC-1 and HRM-VOC-2, demonstrated sensitivity/specificity of 100%/99.29% and 92.86%/99.39%, respectively, for detecting Alpha, 90.08%/100% and 92.31%/100% for Delta, and 93.75%/100% and 100%/99.38% for Omicron BA.1. …”
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Groundwater exploration, management strategies and sustainability: Geophysical approaches by Joseph Omeiza Alao, Fahad Abubakar

“…This review article provides an in-depth analysis of the various roles of geophysics in groundwater development and sustainability, highlighting the importance of geophysical methods in mapping subsurface structures, characterizing aquifer properties, monitoring groundwater levels, detecting contamination, optimizing well siting, and assessing groundwater recharge processes. …”
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ANALYSIS AND MINIMIZATION OF THE RISKS OF HARMFUL DEPENDENCIES IN THE PROCESS OF CONTINUOUS INTEGRATION AND IMPLEMENTATION OF SOFTWARE CODE by Danil N. Alekseev, Renat M. Khamitov

“…To prevent these threats, various protection methods are proposed, such as controlling access to private packages, using automated tools for monitoring and checking dependencies, and implementing machine learning systems to detect suspicious packages. …”
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Evaluation of Applied Destructive and Non-destructive Tests in the Technical Inspection Process of High-Frequency Electric Resistance Weld Pipes by Meysam Rasooly, Masoud Shahi

“…The final result of this essay is the optimization of high-frequency electric resistance welding pipe production. …”
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Application of artificial intelligence medical imaging aided diagnosis system in the diagnosis of pulmonary nodules by Ya Yang, Pan Wang, Chengzhou Yu, Jing Zhu, Jinping Sheng

“…Drawing on the principles and rules of image segmentation methods, the construction and optimization of a medical image-aided diagnosis system is carried out to realize the precision of the diagnosis system in the diagnosis of pulmonary nodules. …”
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Pilot Study on Visual Function and Fundus Autofluorescence Assessment in Diabetic Patients by Ana M. Calvo-Maroto, José J. Esteve-Taboada, Rafael J. Pérez-Cambrodí, David Madrid-Costa, Alejandro Cerviño

“…FAF and optimized-FAF images could be a useful complementary tool for detecting early alterations associated with the development and progression of DR.…”
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Whole genome sequencing completes the molecular genetic testing workflow of patients with Lynch syndrome by Klaudia Horti-Oravecz, Anikó Bozsik, Tímea Pócza, Ildikó Vereczkey, Tamás Strausz, Erika Tóth, Tatiana Sedlackova, Diana Rusnakova, Tomas Szemes, István Likó, Edit Oláh, Henriett Butz, Attila Patócs, János Papp, Vince Kornél Grolmusz

“…Abstract Multigene panel tests (MGPTs) revolutionized the diagnosis of Lynch syndrome (LS), however noncoding pathogenic variants (PVs) can only be detected by complementary methods including whole genome sequencing (WGS). …”
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Enteroviruses in the etiology of acute respiratory viral infections in children, Novosibirsk, Russia by O. G. Kurskaya, T. A. Saroyan, A. R. Nokhova, N. A. Dubovitskiy, A. A. Derko, P. S. Basova, T. A. Gutova, M. V. Solomatina, T. A. Murashkina, K. A. Sharshov

“…Influenza virus was detected in 14.2 % of the examined children. Enteroviruses were detected in 9.2 % of cases and rhinovirus was detected in 10.8 % of the examined children.   …”
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An Updated Model Does Not Reveal Sex Differences in Patellofemoral Joint Stress during Running by Laura Jacobson, C. Nathan Vannatta, Catherine Schuman, Thomas W. Kernozek

“… # Conclusion The inclusion of static optimization to estimate quadriceps muscle force and sex-specific contact area of the patellofemoral joint did not reveal sex differences in patellofemoral joint stress, but differences in non-sagittal plane hip motion were detected. …”
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Transductive Radar Clutter Segmentation Network for Ionospheric Inhomogeneity Clutter Suppression by Yuanbiao Li, Lei Yu, Yinsheng Wei

“…A deep unfolding approach maps the iterative optimization procedure onto a neural network, jointly optimizing across multiple traditional clutter suppression methods. …”
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CVT-HNet: a fusion model for recognizing perianal fistulizing Crohn’s disease based on CNN and ViT by Lanlan Li, Ziyue Wang, Chongyang Wang, Tao Chen, Ke Deng, Hong’an Wei, Dabiao Wang, Juan Li, Heng Zhang

“…In addition, the MobileNetV2 with Coordinate Attention mechanism and encoder modules are optimized to improve the precision of detecting anal fistulas. …”
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Simultaneous Fault Estimation and Fault-Tolerant Consensus Control for Multi-Agent Systems Against Multiple Time Delays by Yimin Liu, Jianliang Chen, Yongli Yang, Hao Fu

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