Assessment of diagnostic potency of exosomal microRNA in circulating blood of patient with thyroid cancer by R. B. Samsonov, V. S. Burdakov, D. A. Rakitina, R. A. Nazhmudinov, Z. A. Radzhabova, D. A. Vasilyev, M. V. Filatov, A. V. Malek

“…Correct and timely differential diagnosis is the basis for correctly choosing a treatment policy and hence determines treatment results. Methods for molecular genetic analysis are being recently developed and introduced into clinical practice, enabling the diagnostic process to be optimized. …”
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Comparative Study of Free Amino Acid Profiles in Underground Organs of Several Species of the Genus Rumex During Different Phases of the Vegetation Cycle by A. M. Poluyanov, U. A. Matvienko, A. Yu. Sokolova, A. E. Savelyeva, N. A. Durnova, N. V. Bobkova

“…Therefore, understanding the dynamics of bioactive compound accumulation in plants throughout their phenological phases of development is critical to optimizing their potential health benefits.Aim. To compare qualitative composition and dynamics of AA accumulation in the underground organs of four representatives of the Rumex genus: R. confertus, R. crispus L., R. obtusifolius L., R. aquaticus L. of three different vegetative phases.Materials and methods. …”
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PD-L1 and p53 expression in squamous cell carcinoma of the oropharynx depending on human papilloma virus status by D. Sh. Polatova, A. Yu. Madaminov, A. V. Savkin, A. I. Nurzhabov, N. K. Asamedinov, D. A. Ibragimova, R. R. Davletov, S. K. Nasirov

“…Easy testing, low cost, reliability, and high sensitivity of immunohistochemical analysis for p16INk4a allowed to widely use this method for Hpv status determination.Aim. To determine the association between programmed death-ligand 1 (pD-L1) and p53 expression and presence of indirect Hpv marker – p16INk4a – in patients with OSCC.Materials and methods. …”
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Prevalence and molecular characterisation of multi-drug resistant ST11 hypervirulent Klebsiella pneumoniae in a teaching hospital by Xue Li, Kai Ma, Xin Liu, Ping Cheng, Lailai Li, Yihui Chai, Mingle Cao, Yuqi Yang

“…Methods We conducted a retrospective analysis of 102 carbapenem-resistant CRKP strains. …”
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Suaeda Salsa Hyperspectral Index (SSHI) for mapping S. salsa in coastal wetlands using hyperspectral satellite imagery: case studies in northern coastal China by Mengyao Zhang, Yinghai Ke, Kun Shang, Zhaojun Zhuo, Han Liu, Peng Li, Nana Zhao, Jinghan Sha, Jinyuan Li

“…To evaluate the performance of SSHI, a simple thresholding method and a random forest (RF) model were used to map S. salsa, respectively. …”
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Portable Impedance Analyzer for FET-Based Biosensors with Embedded Analysis of Randles Circuits’ Spectra by Norman Pfeiffer, Martin Bach, Alice Steiner, Anna-Elisabeth Gerhardt, Joan Bausells, Abdelhamid Errachid, Albert Heuberger

“…The electrochemical impedance spectroscopy (EIS) is a measurement method for characterizing bio-recognition events of a sensor, such as field-effect transistor-based biosensors (BioFETs). …”
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Designing the alignment model of petrochemical projects with the country's industrial strategies in the Ministry of Petroleum by Abdolrasoul Shojaeian, ghanbar abbaspour esfadan, Changiz Valmohammadi, Aboutorab Alirezaei

“…Abstract The purpose of this research is to design a model for the alignment of petroleum refinery projects with the country's industrial strategies in the Ministry of Oil. The research method is applicable in terms of purpose, qualitative in terms of data collection, and data-based type. …”
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A Comprehensive Description of the Roadmap to Identify and Validate a Myelin Biomarker by Giovanna Capodivento, Davide Visigalli, Andrea Armirotti, Chiara Demichelis, Marinella Carpo, Roberto Fancellu, Erika Schirinzi, Daniele Severi, Diego Franciotta, Fiore Manganelli, Gabriele Siciliano, Alessandro Beronio, Elisabetta Capello, Paola Lanteri, Eduardo Nobile-Orazio, Angelo Schenone, Luana Benedetti, Lucilla Nobbio

“…Design: This study adheres to STROBE guidelines for observational cross-sectional studies on human patients and to ARRIVE guidelines for animal models. Method: Following the recommendations of the Society for CSF Analysis and Clinical Neurochemistry, we describe the stepwise process to validate SM as a myelin biomarker, starting from the optimization of the fluorescence-based assay and analytical validation in experimental models until clinical and pathological validation in biological fluids of neurological patients. …”
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STUDY OF BIOCHEMICAL MARKERS’ CONTENT OF BONE TISSUE METABOLISM IN THE ORAL LIQUID OF PATIENTS WITH GENERALIZED PERIODONTITIS by I.V. Vozna, A.V. Samoilenko, S.V. Pavlov

“… The study aims to determine the concentration of vitamin D in the oral fluid, markers of bone destruction as well as to assess the diagnostic value of these indicators to optimize special programs for diagnostics and prevention of major dental diseases in the industrial region’s residents. …”
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Incidence and determinants of excessive weight gain in people living with HIV initiating tenofovir, lamivudine, and dolutegravir-based therapy: a multicenter retrospective study in... by Gashaw Sisay Chanie, Wagaye Atalay, Tirsit Ketsela Zeleke, Zemenu Wube Bayleyegn, Yonas Sisay Aragie, Gizachew Kassahun Bizuneh, Mihret Melese, Rahel Belete Abebe

“…Proactive surveillance in these patient groups is crucial to optimize therapeutic outcomes and address potential health concerns associated with weight changes.…”
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Economic assessment of energy consumption and greenhouse gas emissions from wheat production in Ardabil provience by Jabraeil Taghinazhad, َAdel Vahedi, Fayaz Ranjbar

“…The being Independent on agricultural productions lead to take more attention to modern methods and the objective of all these methods is increasing the performance with the efficient use of inputs or optimizing energy consumptions in agricultural systems. …”
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Letter from editor by Carlos A. Vargas

“…From Morocco, a study aimed to evaluate geostatistical methods for delimitation of anomalies detected in the phosphatic series of Sidi Chenane found that the exponential model is the best one to represent the spatial variability of their geoelectric data; models of the geology were successfully obtained from geostatistical method, which help mapping the phosphate deposit inclusions and the estimations of phosphate reserves were improved and better constrained. …”
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Drop out prediction in sport centres. Definition of models and reproducibility (Predicción de bajas de clients en centros deportivos. Definición de modelos y reproducibilidad) by Iván Clavel San Emeterio, Jorge García-Unanue, Eliseo Iglesias-Soler, Leonor Gallardo, José Luis Felipe

“…Purpose: One of the main problems detected in the sport centre field is abandonment of physical activity by members. …”
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Physicochemical and functional assessment of messenger RNA 5′Cap-end impurities under forced degradation conditions by J. Hutchinson, F. Schweikart, R. Shannon, A. Murthy, S.M. Bates, S. Leone, T. Nissan, D. Sideris, C. Lal, J. Button, R. Rani, G. Thom, N. Bond, E. Örnskov, S. Trabulo

“…Method-induced artifactual degradation occurred during LC-MS data acquisition, emphasizing the importance of using reference standards and optimizing methods to reduce such artifacts. …”
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An AI-driven machine learning approach identifies risk factors associated with 30-day mortality following total aortic arch replacement combined with stent elephant implantation by Shuai Zhang, Lulu Li, Jingyu Wang, Yuan Li, Yongkang Zhou, Yuqing Tao, Cuntao Yu, Xiaogang Sun, Hongwei Guo, Dong Zhao, Yi Chang, Jing Sun, Xiangyang Qian

“…By implementing the new method, the AUC value significantly improved from 0.6981 using the XGBoost model to 0.8687 with the PSO-ELM-FLXGBoost model.Conclusion In this study, machine learning methods were successfully established to predict ATAAD perioperative mortality, enabling the optimization of postoperative treatment strategies to minimize the postoperative complications following cardiac surgeries.…”
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Identifying nontuberculous mycobacteria from a mycobacteria growth indicator tube with nuclear magnetic resonance spectroscopy: A laboratory metabolomics analysis case report by Dr Christoffel Opperman, Dr Marwaan Rylands, Dr Sarishna Singh, Ms Aysha Amien, Mr Yonas Ghebrekristos, Prof Rob Warren, Dr Wynand Goosen

“…However, the complexity of MGIT broth media presents challenges for NMR-based NTM identification, necessitating optimization of sample preparation methods. Therefore, in light of the growing demand for improved NTM diagnostic tools, this laboratory case accentuates the need to contemplate innovative approaches with cutting-edge technology for NTM identification.…”
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Classifying Dry Eye Disease Patients from Healthy Controls Using Machine Learning and Metabolomics Data by Sajad Amouei Sheshkal, Morten Gundersen, Michael Alexander Riegler, Øygunn Aass Utheim, Kjell Gunnar Gundersen, Helge Rootwelt, Katja Benedikte Prestø Elgstøen, Hugo Lewi Hammer

“…Clinical signs and symptoms are currently used to diagnose dry eye disease. Metabolomics, a method for analyzing biological systems, has been found helpful in identifying distinct metabolites in patients and in detecting metabolic profiles that may indicate dry eye disease at early stages. …”
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SEVERE LIVER IRON CONCENTRATIONS (LIC) IN 24 PATIENTS WITH Β-THALASSEMIA MAJOR: CORRELATIONS WITH SERUM FERRITIN, LIVER ENZYMES AND ENDOCRINE COMPLICATIONS by Vincenzo De Sanctis

“…Therefore, physicians’ strategies shall optimize early identification of those patients in order to optimise their chelation therapy and to avoid iron-induced organ damage. …”
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Modular and efficient assembly of CRISPR vectors and construction of an apple gene editing efficiency evaluation system by HOU Feifan, HUANG Chuanmi, HAO Pengbo, CONG Liu, BAI Tuanhui, SHI Jiangli, WANG Miaomiao, LIU Yu, ZHAO Yujie, WAN Ran, SHEN Yawen, ZHANG Hengtao, ZHANG Kunxi, ZHENG Xianbo, JIAO Jian

“…We aim to develop a modular and efficient CRISPR vector system, optimize the Agrobacterium-mediated transient delivery system in apple plantlets, and establish a corresponding evaluation system for assessing the efficiency of gene editing in apple. …”
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Evaluation of Immunogenicity of <i>Mycobacterium tuberculosis ag85ab</i> DNA Vaccine Delivered by Pulmonary Administration by Haimei Zhao, Zhen Zhang, Yong Xue, Nan Wang, Yinping Liu, Xihui Ma, Lan Wang, Xiaoou Wang, Danyang Zhang, Junxian Zhang, Xueqiong Wu, Yan Liang

“…The levels of Th1, Th2, and Th17 cytokines in the culture supernatants of lung and spleen lymphocytes were detected with the Luminex method. The proportion of FoxP3 regulatory T cells in splenocytes was determined by flow cytometry. …”
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