Exploring the role of hyperreflective walls as a biomarker for the management of cystoid macular edema by Zhang-Rong Yang, Chang Li, Dong-Jun Xing, Gao-Xu Wei, Ce-Ce Zhao, Miao Zhou, Xiao-Han Ma, Yi Zhao, Shu Yang, Rong-Guo Yu, Zhi-Qing Li

“…This study suggests that early detection of HRW and optimization of treatment strategies may improve patient prognosis.…”
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Preclinical evaluation of AAV9-coSMN1 gene therapy for spinal muscular atrophy: efficacy and safety in mouse models and non-human primates by Wenhao Ma, Zhijie Wu, Tianyi Zhao, Yan Xia, Jing Qin, Xue Tian, Xin Li, Jun He, Yan Zhang, Lina Zhang, Li Li, Zheyue Dong, Zhichun Feng, Xiaoyan Dong, Wang Sheng, Xiaobing Wu

“…Here, we present the preclinical research that laid the groundwork for these trials, offering comprehensive data on the efficacy and safety of AAV9-coSMN1 in both murine models and non-human primates. Material and method We developed a codon-optimized h SMN1 expression cassette and analyzed SMN protein levels using Western blot and immunofluorescence. …”
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Statistics and behavior of clinically significant extra-pulmonary vein atrial fibrillation sources: machine-learning-enhanced electrographic flow mapping in persistent atrial fibri... by Peter Ruppersberg, Steven Castellano, Philip Haeusser, Kostiantyn Ahapov, Melissa H. Kong, Stefan G. Spitzer, Stefan G. Spitzer, Georg Nölker, Andreas Rillig, Tamas Szili-Torok

“…Notably, the majority of significant sources were not continuously active; however, when these sources switched “ON,” the spatial variability of AF cycle lengths in the respective atrium decreased by more than 50%, suggesting an entraining effect.ConclusionsBy systematically optimizing the EGF Model's hyperparameters based on clinical outcomes, we reliably detect and target key AF sources that, when ablated, improve procedural success. …”
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In-House Validation of Four Duplex Droplet Digital PCR Assays to Quantify GM Soybean Events by Daniela Verginelli, Sara Ciuffa, Katia Spinella, Davide La Rocca, Marisa Misto, Cinzia Quarchioni, Pamela Bonini, Cristiana Fusco, Lorella Peroni, Stefania Peddis, Ugo Marchesi

“…Since the ddPCR methods provide accurate quantification, show less sensitivity to PCR inhibitors and are more suitable for multiplexing compared to the real-time PCR, the optimization of the existing assays was performed with the exception of MON87701, according to the JRC Guidance documents and technical reports. …”
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Early cerebrospinal fluid elevations of pTau-217 in severe traumatic brain injury subjects by Hamad Yadikar, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Firas H. Kobeissy, Claudia Robertson, Spyridoula Tsetsou, John B. Williamson, John B. Williamson, Damon G. Lamb, Damon G. Lamb, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Amy K. Wagner, Todd Kibaugh, Shih-Han Kao, Zhifeng Kou, Zhifeng Kou, Robert D. Welch, Jose-Miguel Yamal, Luis Leon-Novelo, Richard Rubenstein, Richard Rubenstein, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang, Kevin K. W. Wang

“…This study investigated whether cerebrospinal fluid (CSF) phosphorylated Tau at threonine-217 (pTau-217) could serve as an early biomarker for severe TBI (sTBI).MethodsCSF samples from 26 sTBI patients, collected between 6 and 240 h post-injury, and 19 healthy controls were analyzed using an optimized direct enzyme-linked immunosorbent assay (ELISA; sensitivity <4.7 pg/mL) for pTau-217 detection, complemented by Western blot validation. …”
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Study on the Structural Changes of Boneless Chicken Claw Collagen and Its Effect on Water Retention Performance by Zheng Tang, Yiguo He, Jing Zhang, Zhifeng Zhao, Yiming Nie, Xingxiu Zhao

“…The purpose of this study was to explore the water retention mechanism of chicken claws by detecting the structural changes in collagen in boneless chicken claws under different expansion rates. …”
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SARS-CoV-2-specific humoral and cellular immunity assessment in Peruvian vaccinated population: a cross-sectional study by Marlon Yuri Garcia-Paitan, Salyoc Tapia-Rojas, Hector Santiago Alvarez Vega, Javier Enciso-Benavides, Maria J. Pons, Ana Mayanga-Herrera

“…Background Evaluating both humoral and cellular immunity is essential for optimizing vaccination strategies and preventing post-pandemic SARS-CoV-2 outbreaks. …”
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Tumor Microenvironment Responsive Key Nanomicelles for Effective Against Invasion and Metastasis in Ovarian Cancer Using Mice Model by Liu Y, Kong L, Yu Y, Zang J, Zhang L, Guo RB, Li ST, Cheng L, Li XT, Chen YQ

“…Nano-particles, as a novel drug delivery system, have significant potential for improving therapeutic efficacy and overcoming these challenges.Methods: According to the high expression level of matrix metalloproteinase-2 (MMP-2) in the tumor microenvironment, MMP-2 responsive nano-particles (PVGLIG-MTX-D/T-NMs) containing docetaxel and triptolide were prepared by the thin-film dispersion method. …”
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Study on the growth characteristics of Pinus yunnanensis seedlings based on hormonal regulation and chlorophyll metabolism by Ruilian Li, Qibo Wang, Sunling Li, Lin Chen, Nianhui Cai, Yulan Xu

“…These findings can provide valuable references for cultivating high-quality seedlings, optimizing seed source selection, and improving afforestation techniques.…”
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Receptor-Mediated SPION Labeling of CD4⁺ T Cells for Longitudinal MRI Tracking of Distribution Following Systemic Injection in Mouse by Yu Ping, Songyue Han, Brock Howerton, Francesc Marti, Jake Weeks, Roberto Gedaly, Reuben Adatorwovor, Fanny Chapelin

“…We optimized the labeling protocol for maximal CD4⁺ cell labeling and viability. …”
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Global prevalence and contributing factors of nurse burnout: an umbrella review of systematic review and meta-analysis by Addisu Getie, Temesgen Ayenew, Baye Tsegaye Amlak, Mihretie Gedfew, Afework Edmealem, Worku Misganaw Kebede

“…Publication bias was assessed using funnel plots and Egger’s regression test, with the former providing a visual assessment of bias and the latter offering a statistical method to detect asymmetry. Results The global prevalence of nurse burnout was evaluated in three areas: emotional exhaustion (33.45%, 95% CI 27.31–39.59), depersonalization (25.0%, 95% CI 17.17-33.00), and low personal accomplishment (33.49%, 95% CI 28.43–38.55). …”
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CRISPR‐Based Environmental Biosurveillance Assisted via Artificial Intelligence Design of Guide‐RNAs by Benjamín Durán‐Vinet, Jo‐Ann L. Stanton, Gert‐Jan Jeunen, Ulla vonAmmon, Jackson Treece, Xavier Pochon, Anastasija Zaiko, Neil J. Gemmell

“…Environmental DNA (eDNA) has transformed traditional biosurveillance by detecting trace DNA fragments left by organisms in their surroundings, primarily by applying quantitative polymerase chain reaction (qPCR) methods. …”
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Phenotypic screen of early-developing larvae of the blood fluke, schistosoma mansoni, using RNA interference. by M M Mourão, Nathalie Dinguirard, Glória R Franco, Timothy P Yoshino

“…RNA interference (RNAi) represents the only method currently available for manipulating gene-specific expression in Schistosoma spp., although application of this technology as a functional genomic profiling tool has yet to be explored. …”
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Catastrophic anthropogenic vegetation changes in the Kiliyan arm of the Danube Delta (Ukraine) by Dmytro Dubyna, Tetiana Dziuba, Liubov Borsukevych

“…The investigation of the anthropogenic dynamics of vegetation were carried out by direct methods – on stationary and semi-stationary sites. …”
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Assimilation of snow water equivalent from AMSR2 and IMS satellite data utilizing the local ensemble transform Kalman filter by J. Lee, M.-I. Lee, S. Tak, E. Seo, E. Seo, Y.-K. Lee

“…This approach constitutes an objective method that optimally combines two previously unattempted incomplete data sources: the satellite SWE retrieval from the Advanced Microwave Scanning Radiometer 2 (AMSR2) and dynamically balanced SWE from the JULES land surface model. …”
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Multi-step pathway engineering in probiotic Saccharomyces boulardii for abscisic acid production in the gut by Femke Van Gaever, Paul Vandecruys, Yasmine Driege, Seo Woo Kim, Johan M. Thevelein, Rudi Beyaert, Jens Staal

“…In this study, we propose an alternative ABA administration method to overcome these challenges. We genetically engineered a strain of the probiotic Saccharomyces boulardii to produce and deliver ABA directly to the gut of mice. …”
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Proactive dynamic flooding regulations for river basins in China’s arid and semi-arid region of Xinjiang by Xintong Gong, Qiang Zhang, Senlin Tang, Yungang Bai, Vijay P. Singh, Zhenlin Lu

“…We used an improved pre-release constraint algorithm, such as the long-short-series mean correction method, and evaluated the flood stage potential during the aforementioned three intervals. …”
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Radiomic study on preoperative multi‐modal magnetic resonance images identifies IDH‐mutant TERT promoter‐mutant gliomas by Haoyu Wang, Shuxin Zhang, Xiang Xing, Qiang Yue, Wentao Feng, Siliang Chen, Jun Zhang, Dan Xie, Ni Chen, Yanhui Liu

“…A diagnostic model (multilayer perceptron classifier) for detecting the IDHmut pTERTmut gliomas was trained using an automatic machine‐learning algorithm named tree‐based pipeline optimization tool (TPOT). …”
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DNA Barcoding Using 18S rRNA Gene Fragments for Identification of Tick-Borne Protists in Ticks in the Republic of Korea by Badriah Alkathiri, Subin Lee, KyuSung Ahn, So Youn Youn, Mi-Sun Yoo, Hyang-Sim Lee, Yun Sang Cho, Jaeyun Jung, Kwangwon Seo, Soochong Kim, Rika Umemiya-Shirafuji, Xuenan Xuan, Dongmi Kwak, SungShik Shin, Seung-Hun Lee

“…Between 2021 and 2022, questing ticks were collected using the flagging method, with a total of 13,375 ticks collected and pooled into 1003 samples. …”
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The role of diffuse correlation spectroscopy and frequency-domain near-infrared spectroscopy in monitoring cerebral hemodynamics during hypothermic circulatory arrestsCentral Messa... by Alexander I. Zavriyev, BS, Kutlu Kaya, MS, Parisa Farzam, PhD, Parya Y. Farzam, BS, John Sunwoo, PhD, Arminder S. Jassar, MBBS, Thoralf M. Sundt, MD, Stefan A. Carp, PhD, Maria Angela Franceschini, PhD, Jason Z. Qu, MD

“…Conclusions: FDNIRS-DCS is able to detect changes in CBFi, SO2, and CMRO2i with intervention and can become a valuable tool for optimizing cerebral protection during HCA.…”
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