Egyptian pediatric kidney transplantation: highlights on post-transplant follow-up and management of complications by the Egyptian Pediatric Clinical Practice Guidelines Committee... by Bahia Moustafa, Neveen A. Soliman, Ihab Shaheen, Ahmed Badr, Fatma M. Atia, Mohamed K. El Hatw, Engy Adel Mogahed, Mona EL-Ghamrawy, Khaled El Khashab, Noha Shaheen, Mohamad G. Shouman, Abeer Selim, Magdy El-Sharkawy, Tarek Abd El Azeem, Mohamad Esmat, Manal Salman, Bedeir Ali-El-Dein, Mona Abdel Rahim, Muhamed A. El-Hadedy, Moatasem Elsayed Ghoneim, Mai S. Korkor, Tarek E. I. Omar, Yasser Amer, Ashraf Abdel Baky, On Behalf of Egyptian Pediatric Clinical Practice Guideline Committee

“…Aim/objectives This national adapted guideline aims to frame evidence-based post-transplant recommendations adopted or adapted from EB source guidelines to optimize post-transplant patient care. Methods Our guideline is evidence based adapted from four national and international source guidelines with permissions [KDIGO 2020, RA/BTS 2022–2018, EAU 2018] that were appraised with AGREE II instrument tool. …”
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Prototype Mobile Vision System for Automatic Length Estimation of Olive Flounder (<i>Paralichthys olivaceus</i>) in Indoor Aquaculture by Inyeong Kwon, Hang Thi Phuong Nguyen, Paththige Waruni Prasadini Fernando, Hieyong Jeong, Sungju Jung, Taeho Kim

“…Real-time estimation of fish growth offers multiple benefits in indoor aquaculture, including reduced labor, lower operational costs, improved feeding efficiency, and optimized harvesting schedules. This study presents a low-cost, vision-based method for estimating the body length and weight of olive flounder (<i>Paralichthys olivaceus</i>) in tank environments. …”
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Research on APF-Dijkstra Path Planning Fusion Algorithm Based on Steering Model and Volume Constraints by Xizheng Wang, Gang Li, Zijian Bian

“…Again, the three-point arc method based on the steering model is used to determine the location of the predicted points and to smooth the paths in real time while constraining the steering angle. …”
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Newborn Intravenous Injection of Liposomal CRISPR/Cas9 Complex Has No Incidence of Off-Targets or Tumors in Mice by Vinícius Monteagudo, Larissa Cristina Barbosa Flores, Melaine Lopes, Flavia Nathiely Silveira Fachel, Giselle Martins, Marina Siebert, Willian da Silva Carniel, Tuane Nerissa Alves Garcez, Helder Ferreira Teixeira, Ursula Matte, Roberto Giugliani, Guilherme Baldo, Édina Poletto, Roselena Silvestri Schuh

“…<b>Background</b>: Genome editing at specific loci is an innovative therapeutic approach; however, it faces many challenges, so optimizing delivery vectors is essential to enhance the safety and efficacy of the CRISPR/Cas9 system. …”
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Protocol update to: High-throughput scNMT protocol for multiomics profiling of single cells from mouse brain and pancreatic organoids by Santiago Cerrizuela, Oguzhan Kaya, Lukas P.M. Kremer, Andrea Sarvari, Tobias Ellinger, Jannes Straub, Jan Brunken, Andrés Sanz-Morejón, Aylin Korkmaz, Ana Martín-Villalba

“…Summary: Single-cell nucleosome, methylome, and transcriptome (scNMT) sequencing is a recently developed method that allows multiomics profiling of single cells. …”
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Development of a Discrete Algorithm for Interpreting Ground-Penetrating Radar Data in Vertically Heterogeneous Media by Kazizat Iskakov, Almaz Tatin, Natalya Glazyrina, Ainur Kussainova, Nurgul Uzakkyzy, Kakim Sagindykov

“…The gradient of the functional required for optimization is obtained via the numerical solution of an adjoint problem. …”
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Soil eDNA-based droplet digital PCR assay as a new monitoring tool for terrestrial mammals by Marko Đokić, Vida Jojić, Pavle Lukić, Lucija Markulin, Vanja Bugarski-Stanojević

“…This study demonstrates the effectiveness of droplet digital PCR (ddPCR) for identifying vertebrate taxa from soil-derived environmental DNA (eDNA) a methodological combination not previously documented in literature. The protocol is optimized for detecting cryptic species of the lesser blind mole rat (BMR). …”
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Mapping Polyclonal HIV-1 Antibody Responses via Next-Generation Neutralization Fingerprinting. by Nicole A Doria-Rose, Han R Altae-Tran, Ryan S Roark, Stephen D Schmidt, Matthew S Sutton, Mark K Louder, Gwo-Yu Chuang, Robert T Bailer, Valerie Cortez, Rui Kong, Krisha McKee, Sijy O'Dell, Felicia Wang, Salim S Abdool Karim, James M Binley, Mark Connors, Barton F Haynes, Malcolm A Martin, David C Montefiori, Lynn Morris, Julie Overbaugh, Peter D Kwong, John R Mascola, Ivelin S Georgiev

“…Computational neutralization fingerprinting, NFP, is an efficient and accurate method for predicting the epitope specificities of polyclonal antibody responses to HIV-1 infection. …”
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Influence of the thickness of Symbiotic Culture of Bacteria and Yeast on purification and final properties of bacterial cellulose by Federica Daus, Devis Montroni, Laura Pesavento, Martina Bruschi, Anna Liguori, Maria Letizia Focarete

“…This observation highlights the need to adapt the purification method based on SCOBY thickness.…”
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Single- vs double-layer uterine closure of the cesarean scar in niche development: the Nicest StudyAJOG Global Reports at a Glance by Ha Thi Thu Nguyen, PhD, Giang Thi Tra Duong, MD, Dat Tuan Do, PhD, Thuong Thi Huyen Phan, PhD, Duc Anh Tran, MD, Toan Khac Nguyen, MD, Anh Duy Nguyen, PhD

“…Transvaginal ultrasound was used to evaluate uterine ultrasound characteristics. If the niche was detected, 3-dimensional transvaginal ultrasound was applied to measure the niche volume via the Virtual Organ Computer-aided AnaLysis method. …”
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Tracing Iridium Dissolution Pathways in Proton Exchange Membrane Water Electrolyzers at Relevant Current Densities in Real Time by Nico C. Röttcher, Jiahua Zhou, Lukas Löttert, Karl J. J. Mayrhofer, Dominik Dworschak

“…Discrepancies with so far reported findings from full‐cell and half‐cell experiments highlight a lack of understanding of catalyst dissolution and the transportation of dissolved species in different systems. The presented method offers unique insights which will help to study and optimize catalyst dissolution by means of various manufacturing and operation parameters to ultimately improve the stability of catalyst layers for water electrolysis.…”
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Thyroid-stimulating hormone and thyroid hormone levels in association with occupational hazards in male coal miners by Yingshi DAI, Yingjun CHEN, Yingqi LUO, Yanhui LIU, Liuquan JIANG, Fan YANG, Gaisheng LIU, Qingsong CHEN

“…ObjectiveTo investigate the associations between occupational hazard exposure and thyroid-stimulating hormone (TSH) and thyroid hormone levels in male coal mine workers. MethodsA cross-sectional study design was adopted. …”
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Preclinical Study of a Dual-Target Molecular Probe Labeled with ⁶⁸Ga Targeting SSTR2 and FAP by Huanhuan Liu, Xiaojun Zhang, Yue Pan, Jingfeng Zhang, Hui Wen, Cong Zhang, Xiaodan Xu, Guangyu Ma, Ruimin Wang, Jinming Zhang

“…Methods: Using the somatostatin analog TATE and quinoline-based compound FAPI-46 as raw materials, we designed and synthesized ⁶⁸Ga-labeled TATE-46. …”
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Captura de movimiento y reconocimiento de actividades para múltiples personas mediante un enfoque bayesiano by A. Marcos, D. Pizarro, M. Marrón, M. Mazo

“…The resulting hypotheses, automatically selected from the particle distribution, are then refined using non-linear optimization methods with statistical priors. The whole framework is tested using a simple but standard method based on comparing articulated cylindrical models with SfS volumes, taken from several cameras. …”
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Case report: direct suture repair of inferior vena cava to rescue a stab patient with hepatic and caval injury through left hepatectomy and total vascular exclusion by Reina Hirooka, Kyoji Ito, Nobuyuki Takemura, Fuminori Mihara, Norihiro Kokudo

“…This report presents a method of vena caval repair in a case of penetrating retrohepatic IVC injury, requiring hepatic resection and total vascular exclusion (TVE). …”
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Refining Flow Cytometry-based Sorting of Plasma-derived Extracellular Vesicles by Daniele Reverberi, Maria Chiara Ciferri, Nicole Rosenwasser, Alessandro Catino, Giuseppina Poppa, Ilaria Giusti, Vincenza Dolo, Rodolfo Quarto, Sara Santamaria, Monica Colombo, Simona Coco, Roberta Tasso

“…Plasma EVs were isolated via sucrose cushion ultracentrifugation, optimizing purity and yield. Flow cytometry with fluorescence threshold triggering was fine-tuned to detect and sort CD9 + EVs, with instrument calibration and parameter adjustments mitigating swarming and improving sorting accuracy. …”
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Integrative study of pulmonary microbiome and clinical diagnosis in pulmonary tuberculosis patients by Hongli Sun, Qiuyue Chen, Dong Zhang, Long Hu, Song Li, Minya Lu, Yao Wang, Huiting Su, Yi Gao, Jiayu Guo, Ying Zhao, Juan Du, Cun Liu, Han Xia, Yingchun Xu, Xiaojun Ge, Qiwen Yang

“…By optimizing the methods and conducting microbial analyses, the accuracy of metagenomic NGS for detecting M. tuberculosis has been improved.…”
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Discovery and validation of frameshift-derived neopeptides in Lynch syndrome: paving the way for novel cancer prevention strategies by Marta Pineda, Sabela Carballal, María Pellisé, Liseth Rivero, Francesc Balaguer, Fátima Marín, Leticia Moreira, Teresa Ocaña, Manel Juan, Daniel Benitez-Ribas, Georgina Flórez-Grau, Joaquín Castillo-Iturra, Cristina Bayó, Giancarlo Castellano, Hardeep Kumari, Maria Daca-Alvarez, Oswaldo Ortiz, Rebeca Moreira, Julia Canet-Hermida, Capella Gabriel

“…CD8+CD137+ activation markers increased significantly (p=0.037) over time and peptide-specific cells were detected by pentamer staining.Conclusions Our predicted FSDN set has optimal coverage among LS carriers and can induce IFN-γ inflammatory responses in LS-derived TILs, offering an opportunity for vaccine development.…”
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Structural properties evaluation of extracted date fruit (Phoenix dactylifera L.) juice at different extraction temperatures by Afna Thaivalappil Abdul Asiz, Mohammed Tarique, Hassan Abdalla, Oni Yuliarti

“…The juice colour darkened with higher extraction temperatures, while no HMF was detected, suggesting the processing method is safe and preserves juice quality. …”
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Retrieval of Ocean Surface Currents by Synergistic Sentinel-1 and SWOT Data Using Deep Learning by Kai Sun, Jianjun Liang, Xiao-Ming Li, Jie Pan

“…Furthermore, a mesoscale eddy detected in the OSC map retrieved by OSCNet is consistent with the collocated sea surface chlorophyll-a observation, demonstrating the capability of the proposed method in capturing the variability of mesoscale eddies.…”
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