CRISPR/Cas9-mediated PD-1 attenuation enhances tumor infiltrating lymphocyte-based adoptive cellular therapy in humanized-PDX model of hepatocellular carcinoma by Wu Ge, Huaping Liu, Di Wu, Yang Hu, Qi Liang, Muqi Liu, Hao Liu, Jianmin Wu, Juan Zhang, Yao Deng, Kedar Ghimire, Pengfei Rong, Wei Wang, Xiaoqian Ma

“…The phenotypic and functional changes were analyzed by flow cytometry. …”
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Polyphasic taxonomy and genome mining of Desertivibrio insolitus gen. nov., sp. nov., a novel psychrotolerant pectin-degrading actinobacterium isolated from desert soil by Shuai Li, Jun Liu, Huan-Huan He, Jia-Rui Han, Jie Huang, Wen-Hui Lian, Yong-Hong Liu, Lei Dong, Wen-Jun Li

“…The strain exhibited psychrotolerance and pectin-degrading activity, indicating ecological adaptation to cold periods and involvement in organic matter turnover in desert soils. Functional annotation identified genes linked to oxidative and osmotic stress responses, carbon starvation, cold and heat shock, DNA repair, and ribosome hibernation, reflecting resilience to extreme conditions. …”
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Extracellular thiol isomerase ERp5 regulates integrin αIIbβ3 activation by inhibition of fibrinogen binding by Kaifei Sun, Yaqiong Zhang, Aizhen Yang, Yuxin Zhang, Zhenzhen Zhao, Xiaofeng Yan, Yi Lu, Yue Han, Depei Wu, Freda Passam, Jingyu Zhang, Yi Wu

“…Our results provide important evidence that platelet ERp5 negatively regulates platelet activation and thrombus formation, via steric hindrance interfering with integrin αIIbβ3 ligation.…”
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Transcriptome and Metabolome Insights into Key Genes Regulating Fat Deposition and Meat Quality in Pig Breeds by Suthar Teerath Kumar, Yunlong Zheng, Jing Xu, Ziyi Zhao, Qi Zhang, Yunpeng Zhang, Min Li, Hong Zou, Riaz Muhammad Azeem, Wu-Sheng Sun, Yuan Zhao, Shu-Min Zhang

“…In contrast, NR4A3 and PPARGC1A showed a strong negative correlation with most upregulated metabolites. …”
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Tumor suppressor SLC9A2 inhibits colorectal cancer metastasis and reverses immunotherapy resistance by suppressing angiogenesis by Zizhen Zhang, Shengde Liu, Ting Xu, Nan Chen, Cheng Liu, Hong Yang, Yuanmeng Shi, Zhiwei Li, Xujiao Feng, Yanhong Yao, Xiaorui Duan, Gehan Xu, Cheng Zhang, Zhenghang Wang, Jian Li, Lin Shen

“…Additionally, we conducted assays for cell migration, invasion, angiogenesis, and ELISA to evaluate VEGFA production, all to confirm the functional role of SLC9A2. Our findings were further validated in human CRC tissue samples and publicly available datasets to assess the clinical relevance of the identified targets. …”
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ZEB1 stratifies the response to Sorafenib and Mdivi-1 combination therapy in hepatocellular carcinoma by H. Freudenstein, M. Strecker, S. Gylstorff, W. Shi, M. Boettcher, S. Medunjanin, C. Catapano, C. Siba, C. Wex, T. Wartmann, A. Y. Sanin, M. Franz, J. Arend, D. Mougiakakos, M. Pech, R. S. Croner, U. D. Kahlert, F. Stelter

“…Zinc finger E-box binding homeobox 1 (ZEB1) is negative prognostic factor in liver cancer by promoting therapy resistance and tumorigenesis. …”
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TssI2-TsiI2 of Vibrio fluvialis VflT6SS2 delivers pesticin domain-containing periplasmic toxin and cognate immunity that modulates bacterial competitiveness by Yuanming Huang, Yu Han, Zhenpeng Li, Xiaorui Li, Zhe Li, Ping Liu, Xiaoshu Liu, Qian Cheng, Fenxia Fan, Biao Kan, Weili Liang

“…Vibrio fluvialis is a halophilic Gram-negative bacterium regarded as an emerging unusual enteric pathogen of increasing public health concern. …”
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Modeling and regulation of water exchange between the oxbow lake and the middle Yangtze River by Xiaoguang Liu, Shiming Yao, Zhongwu Jin, Bing Ding, Lican Ge, Shuo Guan, Weijie Wang

“…These findings highlight the critical role of hydrological regulation in maintaining the ecological health and functions of oxbow lakes.…”
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Calcium-dependent adhesion protein CDH18, a potential biomarker for prognosis in uterine corpus endometrial carcinoma by Xiaoxuan Tang, Shanxing Dang, Jie Qiu, Ruilan Zhou, Jing Ling, Limei Zhang, Xiaopeng Peng, Qingyun Li, Jin Liu, Wei Liao, Qingxiu Mei, Miao Xie, Yehong Sun, Jianmei Huang, Xuelian Du, Wencong Song

“…Additional analyses included functional enrichment, tumor mutational burden, tumor microenvironment (TME) estimates via ESTIMATE, and immune infiltration assessment to clarify CDH18’s potential mechanisms in UCEC. …”
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Climate resilience differs across dairy sheep populations in Europe by M.J. Carabaño, V. Tsartsianidou, D. Hazard, H. Larroque, D. Buisson, C. Pineda-Quiroga, E. Ugarte, M. Serrano, M.A. Jiménez, A. Triantafyllidis, G. Arsenos, C. Díaz, M. Ramón

“…Reaction norm models with polynomial functions in mixed model settings were fitted. Substantial variability in the population response to changes in TL was observed across breeds. …”
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MiR-216a-3p inhibits the cytotoxicity of primary natural killer cells by Rowan Abdelbary, Rowan Abdelbary, Manon Ragheb, Manon Ragheb, Shereen A. El Sobky, Nagwa El-Badri, Nourhan Aboud, Ahmed Tawheed, Asmaa Gomaa, Mona Zidan, Ramy K. Aziz, Abd Elrahman Abouzid, Radwa Ayman Salah, Mohamed El-Kassas, Imam Waked, Ahmed Moustafa, Ahmed Moustafa, Injie Omar Fawzy, Nada El-Ekiaby, Ahmed Ihab Abdelaziz

“…IntroductionThe role of miRNAs in regulating variable molecular functions has been sought by scientists for its promising utility in regulating the immune response and, hence, in treating various diseases. …”
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Identification of novel molecular subtypes and construction of a prognostic signature via multi-omics analysis and machine learning in lung adenocarcinoma by Ke Ma, Jie Xu, Jie Xu, Congyue Wang, Congyue Wang, Xu Cao, Xu Cao, Wenjie Yu, Jingjing Xi, Xuan Zhang, Jiamin Zhan, Yang Liu, Aoyang Yu, Aoyang Yu, Shuhan Liu, Yanhua Liu, Yanhua Liu, Chong Chen, Chong Chen, Xiaoli Mai, Xiaoli Mai

“…We further confirmed the effects of ANLN knockdown and the expression of a domain-negative anillin protein (dnANLN) via western blotting, cell proliferation assays, flow cytometry, and transwell migration assays in vitro.ResultsOur analysis revealed that the novel molecular subtypes exhibited differences in prognoses, biological functions, and immune infiltration profiles in LUAD. …”
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Targeting adipocyte differentiation with CRT0066101: activation of AMPK signaling in 3T3-L1 cells by Jinque Luo, Jinque Luo, Ling Wang, Ling Wang, Li Zhang, Li Zhang, Wenyu Tang, Meijing Deng, Meijing Deng, Yuxin Shi, Kun Xu, Qingjun Wu, Jieyang Zhao, Jinghuan Zhang, Xin Li, Xin Li

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Correlation analysis of circulating PCDH17 DNA methylation changes with rheumatoid arthritis patients by Fuyu Zhao, Mi Zhou, Jia Liu, Cen Chang, Ping Jiang, Kai Wei, Jianan Zhao, Yu Shan, Yixin Zheng, Yiming Shi, Yunshen Li, Yuejuan Zheng, Qianqian Li, Lei Wang, Huanru Qu, Liangjing Lv, Shicheng Guo, Xinliang Lv, Qi Zhu, Dongyi He

“…This study indicates that PCDH17 cg03865667 methylation may function as a potential biomarker for RA diagnosis. …”
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Increasing the diversity of dietary fibers in a daily-consumed bread modifies gut microbiota and metabolic profile in subjects at cardiometabolic risk by Harimalala Ranaivo, Florence Thirion, Christel Béra-Maillet, Susie Guilly, Chantal Simon, Monique Sothier, Laurie Van Den Berghe, Nathalie Feugier-Favier, Stéphanie Lambert-Porcheron, Isabelle Dussous, Loïc Roger, Hugo Roume, Nathalie Galleron, Nicolas Pons, Emmanuelle Le Chatelier, Stanislav Dusko Ehrlich, Martine Laville, Joël Doré, Julie-Anne Nazare

“…We introduced a larger diversity of consumed DF by using a daily consumed bread in subjects at cardiometabolic risk and assessed the impacts on the composition and functions of gut microbiota as well as on cardiometabolic profile. …”
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The combined effects of extended feeding with a high level of Arthrospira platensis and a commercial enzyme mix or porcine pancreatin on broilers’ blood cells, plasma metabolites a... by Paula A. Lopes, Cristina M. Alfaia, Maria P. Spínola, Rui M. A. Pinto, José M. Pestana, Mónica M. Costa, J. C. Tavares, Miguel P. Mourato, Beatriz Tavares, Daniela F. P. Carvalho, Cátia F. Martins, Madalena Lordelo, José A. M. Prates

“…Furthermore, including A. platensis augmented the concentration of beneficial pigments with antioxidant functions, irrespective of enzyme addition. Most mineral levels remained unaffected (p > 0.05) by A. platensis. …”
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Assessment of Biodegradation Mechanisms of Ceftiofur Sodium by <i>Escherichia</i> sp. CS-1 and Insights from Transcriptomic Analysis by Meng-Yang Yan, Cai-Hong Zhao, Jie Wu, Adil Mohammad, Yi-Tao Li, Liang-Bo Liu, Yi-Bo Cao, Xing-Mei Deng, Jia Guo, Hui Zhang, Hong-Su He, Zhi-Hua Sun

“…Furthermore, the differentially expressed genes (DEGs) of <i>E. coli</i> CS-1 were functionally annotated using a combination of genomics and bioinformatics approaches. …”
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Autoinducer-2 enhances the defense of Vibrio furnissii against oxidative stress and DNA damage by modulation of c-di-GMP signaling via a two-component system by Heng Zhang, Wenjin Zhao, Wenguang Yang, Huimin Zhang, Xinyu Qian, Kai Sun, Qiao Yang, Xihui Shen, Lei Zhang

“…However, unknown AI-2 receptors in bacteria may be more than what has been discovered so far, and there are still many unknown functions for this signal waiting to be explored. Here, we have identified a membrane-bound histidine kinase of the pathogenic bacterium Vibrio furnissii, AsrK, as a receptor that specifically detects AI-2 under low boron conditions. …”
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Metabolome and Transcriptome Analysis Reveals the Regulatory Effect of Magnesium Treatment on EGCG Biosynthesis in Tea Shoots (<i>Camellia sinensis</i>) by Zixuan Feng, Zhuan Li, Rui Yan, Nan Yang, Meichen Liu, Yueting Bai, Yuyuan Mao, Chengzhe Zhou, Yuqiong Guo, Yulin Zeng, Yuhang Ji, Yangshun Lin, Jiayong Chen, Shuilian Gao

“…Epigallocatechin-3-O-gallate (EGCG) is an important ingredient that indicates tea quality and has healthcare functions. Magnesium nutrition can improve the quality and yield of tea plants, but its regulatory role in the biosynthesis of EGCG in tea plants has not been clarified. …”
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Effects of a novel synbiotics-enzyme complex as a replacement for antibiotics on growth performance, slaughter and meat characteristics, immune organ index, and intestinal morpholo... by Zihao Zhao, Zihao Zhao, Simushi Liswaniso, Simushi Liswaniso, Ning Qin, Ning Qin, Shengxiao Cao, Shengxiao Cao, Xin Wu, Xin Wu, Chang Ma, Chang Ma, Chunchi Yan, Chunchi Yan, Rifu Xu, Rifu Xu, Xue Sun, Xue Sun

“…SEC inclusion at 0.1% significantly (P &lt; 0.05) increased the spleen, bursal, and thymus indexes, respectively.ConclusionSupplementation of broilers with 0.1% SEC can be used as an antibiotic alternative because it increases the F/G, improves the carcass and meat quality, increases the body conformation, improves the small intestines' functions, and immune organ size.…”
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