The Diacylglycerol Acyltransferase 3 of <i>Chlamydomonas reinhardtii</i> Is a Disordered Protein Capable of Binding to Lipids Derived from Chloroplasts by Natalia Pavia, Alberto Potenza, Felipe Hornos, José A. Poveda, Gabriela Gonorazky, José L. Neira, Ana M. Giudici, María Verónica Beligni

“…Thermal denaturation, followed by fluorescence or CD, as well as calorimetric denaturation, followed by DSC, did not yield any transition in the pH range where DGAT3 acquired a “native-like” conformation. Furthermore, we used two approaches to demonstrate the interaction of DGAT3 with lipid membranes at the pH at which it had acquired a “native-like” conformation. …”
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Fabrication of 3D-printed coiled PCL microfibrous bundles using alginate-based biocomposites for bone tissue engineering applications by Mohan Pei, Hanjun Hwangbo, GeunHyung Kim

“…Biomedical scaffold fabrication has seen advancements in mimicking the native extracellular matrix through intricate three-dimensional (3D) structures conducive to tissue regeneration. …”
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Converging Electrospinning and 3D-Printing Technologies: From Innovative Design for Tissue Engineering to Global Patent Trends and Technology Transfer by Karen J. Juarez-Navarro, Vincenzo Guarino, Marco A. Alvarez-Perez

“…Electrospinning is a technique that enables the production of nano- and microfibrillar patterns that mimic the native extracellular matrix. However, these nanofibrous structures often lack mechanical properties suitable for reproducing the behavior of structurally complex tissues. …”
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G-Quadruplex Conformational Switching for miR-155-3p Detection Using a Ligand-Based Fluorescence Approach by Pedro Lourenço, Carla Cruz

“…MicroRNA-155-3p (miR-155-3p) is an important biomarker in various pathological conditions, including cancer, making the development of sensitive and specific detection methods crucial. …”
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Alpha-helical destabilization of the Bcl-2-BH4-domain peptide abolishes its ability to inhibit the IP3 receptor. by Giovanni Monaco, Elke Decrock, Koen Nuyts, Larry E Wagner, Tomas Luyten, Sergei V Strelkov, Ludwig Missiaen, Wim M De Borggraeve, Luc Leybaert, David I Yule, Humbert De Smedt, Jan B Parys, Geert Bultynck

“…By comparing the structural and functional properties of the Bcl-2-BH4-IV/GG peptide with its native counterpart, we found that the variant contained reduced α-helicity, neither bound nor inhibited the IP 3R1 channel, and in turn lost its anti-apoptotic effect. …”
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3D bio-printed proteinaceous bioactive scaffold loaded with dual growth factor enhanced chondrogenesis and in situ cartilage regeneration by Prayas Chakma Shanto, Seongsu Park, Md Abdullah Al Fahad, Myeongki Park, Byong-Taek Lee

“…Importantly, the in vitro, in vivo, and in silico simulation showed that the scaffolds promote stem cell recruitment, migration, proliferation, and ECM deposition, and synergistic effects of TGF-β1/FGF-18 with the bioactive scaffolds significantly regulate stem cell chondrogenesis by activating the PI3K/AKT and TGFβ1/Smad4 signaling pathways. After implantation, the proteinaceous bioactive scaffold led to the regeneration of mechanically robust, full-thickness cartilage tissue that closely resembles native cartilage. …”
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Emerging role of the calcium-activated, small conductance, SK3 K+ channel in distal tubule function: regulation by TRPV4. by Jonathan Berrout, Mykola Mamenko, Oleg L Zaika, Lihe Chen, Wenzheng Zhang, Oleh Pochynyuk, Roger G O'Neil

“…Fluorescent assessment of cell membrane potential in native, split-opened CCD, demonstrated that selective activation of the Ca2+-permeable TRPV4 channel, thereby inducing Ca2+ influx and elevating intracellular Ca2+ levels, activated both the SK3 channel and the BK channel leading to hyperpolarization of the cell membrane. …”
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TIM-3 plays a more important role than PD-1 in the functional impairments of cytotoxic T cells of malignant Schwannomas by Zhao Li, Xiaobing Liu, Rongbin Guo, Pengfei Wang

“…Here, we investigated the possibility of using cytotoxic T cells to treat malignant Schwannoma, a rare but aggressive nerve sheath tumor, by examining the native T-cell immunity in the host. We found that compared to CD8 + T cells from healthy controls or benign Schwannoma patients, the CD8 + T cells from malignant Schwannoma patients were present at normal frequencies but were substantially enriched with PD-1 − TIM-3 + and PD-1 + TIM-3 + cells. …”
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A workflow for extracting ungulate trails in wetlands using 3D point clouds obtained from airborne laser scanning by Jinhu Wang, Perry Cornelissen, W. Daniel Kissling

“…Airborne laser scanning (ALS) provides an alternative method because Light Detection and Ranging (LiDAR) can record returns from both the canopy and the ground, as some laser pulses pass through gaps in the vegetation, resulting in highly accurate and dense three-dimensional (3D) point clouds. Here, we present a workflow for extracting ungulate trails using 3D point clouds obtained from country-wide ALS surveys, illustrated by red deer trampling in reedbeds within a 36 km2 marsh area of a Dutch nature reserve. …”
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Lactobacillus johnsonii alleviates colitis by TLR1/2-STAT3 mediated CD206+ macrophagesIL-10 activation by Ding-Jia-Cheng Jia, Qi-Wen Wang, Ying-Ying Hu, Jia-Min He, Qi-Wei Ge, Ya-Dong Qi, Lu-Yi Chen, Ying Zhang, Li-Na Fan, Yi-Feng Lin, Yong Sun, Yao Jiang, Lan Wang, Yan-Fei Fang, Hui-Qin He, Xiong-E Pi, Wei Liu, Shu-Jie Chen, Liang-Jing Wang

“…L. johnsonii could activate native macrophages into CD206+ macrophages and release IL-10 through TLR1/2-STAT3 pathway to relieve experimental colitis. …”
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The Effect of 3′,4′-Methylenedioxychalcone Derivatives on Mycelial Growth and Conidial Germination of <i>Monilinia fructicola</i>: An In Silico and In Vitro Study by Catalina Ferreira, Valentina Silva, Evelyn Muñoz, Gissella Valle, Manuel Martínez-Lobos, Francisca Valdés, Katy Díaz, Iván Montenegro, Patricio Godoy, Nelson Caro, Alejandro Madrid

“…The aim of this study was to synthesize 3′,4′-methylenedioxychalcone derivatives and evaluate their in vitro inhibitory effect on mycelial growth and the conidial germination of <i>M. fructicola</i>. …”
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Feasibility of relaxation along a fictitious field in the 2nd rotating frame (TRAFF2) mapping in the human myocardium at 3 T by Joao Tourais, Joao Tourais, Joao Tourais, Maša Božić-Iven, Maša Božić-Iven, Maša Božić-Iven, Yidong Zhao, Qian Tao, Iain Pierce, Iain Pierce, Christian Nitsche, Christian Nitsche, George D. Thornton, George D. Thornton, Lothar R. Schad, Lothar R. Schad, Thomas A. Treibel, Thomas A. Treibel, Sebastian Weingärtner, Mehmet Akçakaya, Mehmet Akçakaya

“…PurposeEvaluate the feasibility of quantification of Relaxation Along a Fictitious Field in the 2nd rotating frame (RAFF2) relaxation times in the human myocardium at 3 T.MethodsTRAFF2 mapping was performed using a breath-held ECG-gated acquisition of five images: one without preparation, three preceded by RAFF2 trains of varying duration, and one preceded by a saturation prepulse. …”
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Comparison of sub-canopy topsoil properties between three woody plant species, a case study of the Baladeh Watershed, Mazandaran Province, Iran by Alireza Mohebali, Reza Erfanzadeh, Mohammad Jafari

“…Therefore, we studied the effects of three native woody plants with different structural canopies (evergreen needle-leaved vs. deciduous broad-leaved and cushion vs. free canopy) on the nutrient topsoil properties. …”
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BISMUTH MINERALS OF THE NAZAROVSKOE GOLD POLYMETALLIC DEPOSIT (WESTERN TRANSBAIKALIA) by Yu.A. Ryzhkova, K.A. Novoselov, M.A. Rassomakhin, E.A. Rozhkova

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Enhancing Vertical Application and Network Co-Design: A Solution for Multipath Channel Switching in ROS2 With 3GPP Integration by Geza Szabo

“…In this paper, we address the challenging issue of co-designing the Vertical Application Layer (VAL) with Third Generation Partnership Project (3GPP) and non-3GPP networks. This complex problem involves the cooperation of at least the above three distinct domains. …”
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Poly(lactic acid) and its composites as functional materials for 3-D scaffolds in biomedical applications: A mini review of recent trends by S. T. Sikhosana, T. P. Gumede, N. J. Malebo, A. O. Ogundeji

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1’- Acetoxychavicol acetate Suppresses Osteosarcoma Cell Proliferation through the PI3K Pathway: A Molecular Docking and Cytotoxicity Study by Putri Gita Ayu Safitri, Muhammad Da’i, Febri Wulandari

“…In the bioinformatics test, top five target genes identified were SRC, GNAI1, PIK3CD, PIK3CB, PIK3R3. Molecular docking analysis showed that, the native PI3KD ligand showed a strong binding affinity of -10.99 kcal/mol and interacted with more amino acid residues. …”
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Enhancing Gen3 for clinical trial time series analytics and data discovery: a data commons framework for NIH clinical trials by Meredith C. B. Adams, Meredith C. B. Adams, Meredith C. B. Adams, Colin Griffin, Hunter Adams, Stephen Bryant, Robert W. Hurley, Robert W. Hurley, Robert W. Hurley, Umit Topaloglu, Umit Topaloglu

“…This work presents a framework for enhancing Gen3, an open-source data commons platform, with temporal visualization capabilities for clinical trial research. …”
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A comparison of human skeletal muscle cell maturation in 2D versus 3D culture: A quantitative proteomic study by Benjamin R. Tollitt, Samantha W. Jones, Jessica Ohana, James R. Henstock, Malcolm J. Jackson, Anne McArdle

“…Abstract Compared with 2D monolayers, 3D models more closely mimic native muscle tissue and allow functional measurements. …”
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Integration of biomimetic organoid-on-chip and 2D models advances the mechanistic Understanding of STEAP3-mediated regulation in intestinal viral infection by Yi-Wen Chen, Huan-Jung Chiang, Kuan-Ting Liu, Chun-Wei Kao, Shan-Ren Xie, Chao-Ming Su, Yu-Yin Shih

“…To address these limitations, we established an integrative platform that combines the strengths of both 2D and three-dimensional (3D) models to investigate the role of six-transmembrane epithelial antigen of prostate 3 (STEAP3), a membrane ferrireductase, in regulating viral infection in human intestinal epithelium. …”
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