Functionality of bacterial communities in constructed wetlands used for water purification: influence of root components and seasonality by Yao Huang, Yao Huang, Weili An, Weili An, Tianzhu Ning, Zhiguang Ma, Yuelin Li, Ke Liu, Lingbo Ji, Lingbo Ji, Hongxiao Liu, Dafeng Hui, Hai Ren

“…., rhizosphere, rhizoplane, and endosphere) and their impact on water purification is still limited.MethodsTo address this knowledge gap, we employed high-resolution 16S rRNA deep amplicon sequencing to explore the bacterial community structure and assembly within the root components of three plant species (i.e. …”
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Longitudinal maternal glycemia during pregnancy and placental epigenetic age acceleration by Tesfa Dejenie Habtewold, Prabhavi Wijesiriwardhana, Richard J. Biedrzycki, Cuilin Zhang, Katherine L. Grantz, Jagteshwar Grewal, Fasil Tekola-Ayele

“…This study investigated the association between maternal glycemic levels and placental epigenetic age acceleration (PAA) in a multiethnic cohort. Methods In a sample of 301 pregnant women (102 Hispanic, 77 White, 72 Black, and 50 Asian/Pacific Islander), the association of glycemic markers cumulative exposure with PAA was tested using linear regression adjusting for fetal sex, maternal age, educational status, and health insurance status. …”
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Altered thermal preference by preoptic estrogen receptor alpha neurons in postpartum females by Nan Zhang, Meng Yu, Qianru Zhao, Bing Feng, Yue Deng, Jonathan C. Bean, Qingzhuo Liu, Benjamin P. Eappen, Yang He, Kristine M. Conde, Hailan Liu, Yongjie Yang, Longlong Tu, Mengjie Wang, Yongxiang Li, Na Yin, Hesong Liu, Junying Han, Darah Ave Threat, Nathan Xu, Taylor Smiley, Pingwen Xu, Lulu Chen, Tianshu Zeng, Yanlin He, Chunmei Wang

“…Objective: This study aims to investigate how reproductive experience (RE) alters thermal preference and thermoregulation in female mice, with a focus on estrogen receptor alpha (ERα)-expressing neurons in the preoptic area (POA). Methods: Thermal preference and body temperature were measured in female mice with and without RE, and virgin female mice with selective deletion of ERα from the POA (ERαPOA-KO). …”
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Efficacy of Adjunctive Antiseptic Lavage Solution in Managing Acute Hip/Knee Prosthetic Joint Infection: A Comparative Study in a Tertiary Revision Center by Jonathan Quinn, FRACS, Bernard H. van Duren, DPhil, FRCS, Reshid Berber, PhD, FRCS, Mark Higgins, FRCS, Hosam E. Matar, FRCS, Andrew R. Manktelow, FRCS, Benjamin V. Bloch, FRCS

“…We investigated the infection eradication rates of DAIR procedures at our center using Bactisure wound lavage. Methods: A retrospective consecutive review of DAIR procedures for hip and knee PJI was conducted between 2018 and 2023 with a minimum 12-month follow-up at our tertiary revision arthroplasty center. …”
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Instruments for assessing patient-reported experience measures among patients with diabetes mellitus: a scoping review by Soe Sandi Tint, Myo Zin Oo, Nida Buawangpong, Wichuda Jiraporncharoen, Nutchar Wiwatkunupakarn, Kittipan Rerkasem, Kanokwan Kulprachakarn, Hataichanok Chuljerm, Timothy E. O’Brien, Rohini Mathur, Chaisiri Angkurawaranon

“…This review aimed to identify available instruments for assessing patient-reported experiences in individuals with diabetes and examine the different domains, items, and the validity and reliability of these instruments. Methods Following PRISMA-ScR guidelines, databases including PubMed, Embase, CINAHL, Cochrane, and Scopus were searched for English-language articles without year limitations. …”
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Preservation of cfRNA in cytological supernatants for cfDNA & cfRNA double detection in non‐small cell lung cancer patients by Yidan Ma, Yifei Wang, Lei He, Jun Du, Lin Li, Zhixin Bie, Yuanming Li, Xiaomao Xu, Wei Zhou, Xiaonan Wu, Li Yang, Jing Di, Chenyang Li, Xiaoguang Li, Dongge Liu, Zheng Wang

“…The protection of cell‐free RNA (cfRNA) is critical for resolving the issue. Methods A protective solution (PS) was applied for preserving cfRNA in cytological supernatant (CS), and the quality of protected cfRNA was assessed by cycle threshold (CT) values from reverse transcription quantitative polymerase chain reaction (RT‐qPCR). …”
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Malignant phyllodes tumors with sarcomatous components: A histopathologic and molecular study by Ting Lei, Yunjie Song, Zhiyi Shen, Yongqiang Shi, Cunyan Xia, Xu Deng, Wenyue Da, Yan Peng, Qing Li

“…A more comprehensive characterization of their clinicopathological features and molecular profiles may offer significant potential innovative therapeutic strategies. Methods: A total of ten cases were collected with eight cases undergoing DNA next-generation sequencing (NGS). …”
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Individualized resting-state functional connectivity abnormalities unveil two major depressive disorder subtypes with contrasting abnormal patterns of abnormality by Keke Fang, Lianjie Niu, Baohong Wen, Liang Liu, Ya Tian, Huiting Yang, Ying Hou, Shaoqiang Han, Xianfu Sun, Wenzhou Zhang

“…However, the clinical heterogeneity, likely stemming from diverse neurobiological disturbances, complicates findings from standard group comparison methods. This variability has driven the search for MDD subtypes using objective neuroimaging markers. …”
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Hooks, Lines, and Sinkers: How Active Galactic Nucleus Feedback and Cosmic-Ray Transport Shape the Far-infrared–Radio Correlation of Galaxies by Sam B. Ponnada, Rachel K. Cochrane, Philip F. Hopkins, Iryna S. Butsky, Sarah Wellons, N. Nicole Sanchez, Cameron Hummels, Yue Samuel Lu, Dušan Kereš, Christopher C. Hayward

“…We present the first reproduction of the z  ∼ 0 FRC using detailed synthetic observations of state-of-the-art cosmological zoom-in simulations from the Feedback in Realistic Environments (FIRE-3) suite with explicitly evolved CR proton and electron (CRe) spectra, for three models for CR transport and multichannel active galactic nucleus (AGN) feedback. …”
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Expert Consensus on the Management of Acute Otitis Media in India: A Delphi Study by Tanu Singhal, Shyam Kukreja, Jaydeep Choudhury, Vijay Yewale, Balasubramanian Sundaram, Jagdish Chinnappa, Suhas Prabhu, Balaji Veeraraghavan, Camilla Rodrigues, Vidya Krishna, Winsley Rose, Samir Bhargava

“…This expert consensus using the Delphi methodology was undertaken to deliberate and consolidate statements to guide the management of AOM and OME in India. Methods: A panel of 12 experts comprising pediatric infectious disease specialists, clinical microbiologists, and ear, nose, and throat (ENT) specialists participated in a three-step modified Delphi study conducted between April 2023 and January 2024. …”
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Youth experiencing homelessness at risk for suicide: psychosocial risk factors and service use patterns by Laura J. Chavez, Kelly J. Kelleher, Alicia Bunger, Xin Feng, Margaret Fitzpatrick, Jodi L. Ford, Allen B. Mallory, Arielle H. Sheftall, Tansel Yilmazer, Alexis Pizzulo, Jesse L. Hawke, Mattina A. Davenport, Natasha Slesnick

“…The objective of this study is to examine service use and psychosocial risk factors for suicide, in relation to suicide risk assessment, to inform strategies for engaging youth in suicide prevention services. Methods A cross-sectional analysis of youth enrolled in a supportive housing randomized clinical trial. …”
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Pulmonary hypertension in Finland 2008-2020: A descriptive real-world cohort study (FINPAH) by Markku Pentikäinen, Piia Simonen, Helena Tuunanen, Pauliina Leskelä, Terttu Harju, Pertti Jääskeläinen, Christian Asseburg, Minna Oksanen, Erkki Soini, Christina Wennerström, Airi Puhakka, Terttu Harju, Elina Heliövaara, Pertti Jääskeläinen, Katriina Kahlos, Pentti Korhonen, Tiina Kyllönen, Pauliina Leskelä, Kirsi Majamaa-Voltti, Piia Simonen, Anu Turpeinen, Helena Tuunanen, Ville Vepsäläinen, Tapani Vihinen

“…Background: To assess characteristics, risk group distribution, and prognosis of patients with pulmonary arterial hypertension (PAH) or chronic thromboembolic pulmonary hypertension (CTEPH) in Finland. Methods: Clinical chart review of patients with PAH or CTEPH recorded between 2008 and 2019 and linkage to official mortality data. …”
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Duodenal Organoids From Metabolic Dysfunction-Associated Steatohepatitis Patients Exhibit Absorptive and Barrier Alterations by Alia Hadefi, Morgane Leprovots, Gilles Dinsart, Maryam Marefati, Marjorie Vermeersch, Daniel Monteyne, David Pérez-Morga, Anne Lefort, Frédérick Libert, Laurine Verset, Claire Liefferinckx, Christophe Moreno, Jacques Devière, Eric Trépo, Marie-Isabelle Garcia

“…Here, we investigated whether the duodenal epithelium of MASH patients could exhibit intrinsic dysfunctions. Methods: Duodenal epithelial organoids were generated from 16 MASH patients and 14 healthy controls. …”
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Classification of risk for transmission of vaccine-preventable diseases in Brazilian municipalities: comparative analysis before and after the national movement for vaccination and... by Ana Catarina de Melo Araújo, Thales Philipe Rodrigues da Silva, Janaina Fonseca Almeida Souza, Krishna Mara Rodrigues Freire, Fernanda Penido Matozinhos, Eder Gatti Fernandes

“…Abstract Objective To analyze the classification of the risk of transmission of vaccine-preventable diseases in Brazilian municipalities before and after the National Movement for Vaccination and Multivaccination proposed by the Ministry of Health. Methods This is an epidemiological, ecological study using secondary data on vaccination coverage, dropout rates, and homogeneity of vaccination coverage (HCV) for ten immunobiologicals recommended for children under 2 years of age. …”
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Study protocol for an observational cohort study of heat stress impacts in pregnancy in The Gambia, West Africa [version 2; peer review: 1 approved, 2 approved with reservations] by Jane E Hirst, Ana Bonell, Abdul Sesay, Leonidas G. Ioannou, Bubacarr Bah, Kris A. Murray, Andy Haines, Sophie E. Moore, David Jeffries, Neil S Maxwell, Ana Vicero-Cabrera, Apolline Saucy, Cally Tan, Emmanuel Okoh, Dorothy Watters, Bakary Sonko, Williams Oluwatosin Adefila, Yahaya Idris, Mam Leigh-Nabou, Jarra Manneh, Andreas Flouris, Sainabou Bojang, Amanda N Sferruzzi-Perri, Andrew Prentice

“…Microclimate mapping of the area of recruitment will give detailed exposure measurements. Participants will be asked to wear a watch-style device at 28- and 35-weeks gestational age to evaluate maternal heart rate, activity and sleep. …”
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MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair by Jiandong Zhang, Lei Ma, Limin He, Quanxiao Xu, Yan Ding, Lidong Wang

“…Objective: This study was performed to explore the regulatory effects of microRNA-541-3p on TNFRSF10B and TGFβ1 signaling. Methods: Mouse alveolar epithelial cells were transfected with a vector expressing microRNA-541-3p to regulate expression of target genes. …”
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Early therapeutic drug monitoring of methotrexate and its association with acute kidney injury: A retrospective cohort study by Nicolás Tentoni, Miriam Hwang, Gabriela Villanueva, Ryan Combs, Jennifer Lowe, Laura B. Ramsey, Zachary L. Taylor, Thais Murciano Carrillo, María Dolores Aumente, Teresa López‐Viñau López, Carmelo Rizzari, Scott C. Howard

“…This study sought to determine whether early elimination patterns of MTX would be useful as a biomarker of AKI in HDMTX treatment. Methods This retrospective cohort study included two sites that collected ≥2 MTX levels within 16 h from completion of MTX infusion. …”
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Impact of empiric antibiotics on risk of Clostridioides difficile—a causal inference observational analysis by Matthew A. Pappas, Shoshana J. Herzig, Andrew D. Auerbach, Abhishek Deshpande, Eunice Blanchard, Michael B. Rothberg

“…We estimated the increased risk of hospital-onset CDI with one daily dose-equivalent (DDE) of various empiric antibiotics compared to management without that daily dose-equivalent. Methods: Using a multicenter retrospective cohort of adults admitted between March 2, 2020 and February 11, 2021 for the treatment of SARS-CoV-2, we used a series of three-level logistic regression models to estimate the probability of receiving each of several antibiotics of interest. …”
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Unsteady ground: understanding the problem of BPPV in the world of sports by Wojciech Nowak, Mirosław Sawicki, Jagoda Mikołajczyk, Paulina Krzemińska, Maciej Gołębski, Zuzanna Kukla, Sebastian Musialik, Stella Mieruszyńska, Jakub Włosiański, Izabela Sadowska

“…It is widely believed though that certain types of movement may play a role in triggering BPPV. Material and methods: This article is based on a comprehensive review of the literature, conducted using PubMed and Google Scholar. …”
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Oridonin: A natural terpenoid having the potential to modulate apoptosis and survival signaling in cancer by Abhishek Chauhan, Hemant Joshi, Divya Kandari, Diwakar Aggarwal, Ritu Chauhan, Hardeep Singh Tuli, Arpit Mehrotra, Abhilasha Sood, Ujjawal Sharma, Darin Mansor Mathkor, Shafiul Haque, Naveen Chandra Joshi, Laurent Dufossé

“…Purpose: This article investigates the apoptotic mechanisms and anticancer potential of oridonin, including its modulatory effects on signaling pathways and its derivatives' enhanced efficacy against drug-resistant cancer cells. Methods: A systematic literature search was conducted using Scopus, PubMed, SpringerLink, ScienceDirect, Wiley Online, and Web of Science databases, covering the period from 2007 to 2024. …”
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