Identification and predictive machine learning models construction of gut microbiota associated with lymph node metastasis in colorectal cancer by Yongzhi Wu, Chengen Deng, Zigui Huang, Yongqi Huang, Chuanbin Chen, Mingjian Qin, Zhen Wang, Fuhai He, Shenghai Liu, Rumao Zhong, Jun Liu, Chenyan Long, Jungang Liu, Weizhong Tang, Xiaoliang Huang

“…By conducting 16S rRNA sequencing on fecal samples from 147 CRC patients and combining it with the linear discriminant analysis effect size algorithm, we successfully identified significant differences in the gut microbiota between patients with LNM and those with no lymph node metastasis (NLNM). …”
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Feasibility of the Implementation of Tools for Heart Failure Risk Prediction by Michael C. Wang, MD, Bridget Dolan, PharmD, Xiaoning Huang, PhD, Priya M. Freaney, MD, Benjamin H. Freed, MD, Lourdes Vega, PharmD, Nikola Markoski, PharmD, MBA, Amy Wainright, PharmD, Bonnie Kane, BS, RDCS, Laura E. Seegmiller, MPH, Sanjiv J. Shah, MD, Clyde W. Yancy, MD, MSc, Ian J. Neeland, MD, Hongyan Ning, MD, MS, Donald M. Lloyd-Jones, MD, ScM, Sadiya S. Khan, MD, MSc

“…The primary outcome, change in BNP at 1 year, was examined using linear mixed models. Secondary outcomes included change in hs-cTn and traditional risk factor levels. …”
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Evaluation of aggrephagy markers in myofibrillar myopathies by Eliana Iannibelli, Alessandra Ruggieri, Antonello Maruotti, Franco Salerno, Marta Cheli, Alessandra Carnazzi, Lucia Nicolini De Gaetano, Giorgia Riolo, Sara Bortolani, Pietro Riguzzi, Sara Vianello, Gioia Merlonghi, Luca Bello, Matteo Garibaldi, Massimiliano Filosto, Stefano Carlo Previtali, Giorgio Tasca, Gaetano Vattemi, Paola Tonin, Elena Pegoraro, Sara Gibertini, Lorenzo Maggi

“…We analyzed the association between positivity levels of these markers, measured as percentage of positive areas, and selected clinical features utilizing generalized linear mixed models with gamma distribution as the probability model and center-specific random effects to better capture possible heterogeneity across participating centers. …”
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A diffusion tensor imaging-based multidimensional study of brain structural changes after long-term high-altitude exposure and their relationships with cognitive function by Ning Liu, Li Feng, Shuangwei Chai, Hailong Li, Yuanyuan He, Yongyue Guo, Xin Hu, Hengyan Li, Xiangwei Li, Zan Zhou, Xiaomei Li, Yonghong Huang, Wanlin He, Xiaoqi Huang, Xiaoqi Huang, Yunhong Wu, Jinli Meng

“…Moreover, the partial correlation method (age and sex as covariates) was used to analyze the correlations between the intergroup differences in the DTI parameters and a series of clinical indicators of emotional state and cognitive function. Two-sample t tests, Mann-Whitney U test, generalized linear model, or chi-square tests were used for statistical analysis.ResultsCompared with those individuals in the plain group, the scores on the PSQI, SDS, SAS, PHQ-9, and GAD-7 of individuals in the high-altitude group were higher, while the scores on the DST-Backwards, MoCA, and MMSE in the high-altitude group were lower. …”
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Exposure to high temperature and sleep in preadolescents from two European birth cohorts by Esmée Essers, Laura Granés, Núria Botella, Sami Petricola, Llúcia González-Safont, Ane Arregi, Oscar Vegas, Martine Vrijheid, Carmen Iñiguez, Hanan El Marroun, Henning Tiemeier, Mònica Guxens

“…Self-reported daytime sleepiness was assessed using the Paediatric Daytime Sleepiness Scale or the Sleep Disturbances Scale for Children. We ran linear mixed models for each physiological sleep characteristic and distributed lag models for daytime sleepiness. …”
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Prenatal exposure to polyfluoroalkyl substances and visual acuity in preschool children: Findings from the Guangxi Zhuang birth cohort in China by Lidi Lei, Fangfang Lv, Peini Lu, Caimei Mo, Jinxiu Li, Xuemei Xu, Gangjie Wei, Xuanqian Huang, Xiaoqiang Qiu, Dongping Huang, Xiaomei Zhou, Hui Wei, Xiaoyun Zeng, Shun Liu

“…Their relationship still needs to be elucidated in future studies, using larger samples and accounting for both prenatal and childhood periods.…”
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Fecal microbiota transplantation alters the proteomic landscape of inflammation in HIV: identifying bacterial drivers by Claudio Díaz-García, Elena Moreno, Alba Talavera-Rodríguez, Lucía Martín-Fernández, Sara González-Bodí, Laura Martín-Pedraza, José A. Pérez-Molina, Fernando Dronda, María José Gosalbes, Laura Luna, María Jesús Vivancos, Jaime Huerta-Cepas, Santiago Moreno, Sergio Serrano-Villar

“…Proteomic changes in 345 inflammatory proteins in plasma were quantified using the proximity extension assay, with samples collected at baseline and at weeks 1, 8, and 24. …”
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Establishment and Application of MCRV Detection Method for Larvae of Mud Crab, Scylla paramamosain by Xiyu JIN, Shouhu LI, Yue WU, Xu CHU, Wei WANG, Wenhong FANG, Xincang LI

“…When MCRV, MCDV, WSSV, DIV1, EHP, and Vibrio parahaemolyticus nucleic acid samples were used as templates for MCRV detection, no specific amplification was observed except for MCRV samples, indicating that this detection method is highly specific for MCRV. …”
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Gut microbiome and metabolome profiling in coal workers’ pneumoconiosis: potential links to pulmonary function by Xiao Yu, Tao Xiong, Lu Yu, Gaisheng Liu, Fan Yang, Xueqin Li, Yangyang Wei, Xiaojing Wang, Shuting Wei, Yi Jiang, Xiaomei Kong, Shouan Ren, Yiwei Shi

“…After adjusting for age using multifactorial linear regression analysis (MaAsLin2), the distinct gut microbiome profile in CWP patients revealed an increased presence of pro-inflammatory microorganisms such as Klebsiella and Haemophilus. …”
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Assessing the impact of the iPeer2Peer program for adolescents with juvenile idiopathic arthritis: a mixed-methods randomized controlled trial by Fareha Nishat, Lauren Kelenc, Roberta Berard, Ciaran Duffy, Brian Feldman, Paula Forgeron, Adam M. Huber, Nadia Luca, Heinrike Schmeling, Lynn Spiegel, Lori Tucker, Karen Watanabe-Duffy, Tieghan Killackey, Chitra Lalloo, Brittany Wiles, Anya Nair, Sofia Olaizola, Brenna McDermott, Farideh Tavangar, Sara Ahola Kohut, Jennifer N. Stinson

“…These were assessed using linear mixed effects models. Content analysis of semi-structured interviews and focus groups was used to assess satisfaction with the program with mentors and mentees upon study completion. …”
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Music therapy for the treatment of anxiety: a systematic review with multilevel meta-analysesResearch in context by Martina de Witte, Sonja Aalbers, Annemieke Vink, Stijn Friederichs, Anne Knapen, Thomas Pelgrim, Amit Lampit, Felicity A. Baker, Susan van Hooren

“…The secondary measures were outcome, study, sample, or intervention design factors that moderate the effects of music therapy interventions. …”
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Effect of communicating genetic risk of type 2 diabetes and wearable technologies on wearable device-measured behavioural outcomes in East Asians: protocol of a randomised controll... by Youngwon Kim, Shan Lou, Brian Chung, Simon Griffin, Ziyuan Chen, Job G Godino, Flora Lai Tung Cheung, Michael Multhaup, Derwin King Chung K C Chan, Harrison Hin Sheung Ho, Tsz Him Timothy Tse, Shiu Lun Ryan Au Yeung, Joni H Zhang, Mengyao Wang

“…Between-group differences in the continuous and categorical variables collected at baseline will be examined using Analysis of Variance (ANOVA) and χ2 tests, respectively. A series of linear mixed effects models with fixed effects of time, group and interaction between time and group will be performed, with adjustment for potential confounders.Ethics and dissemination The study protocol has undergone review and received approval from the ethics committee of the University of Hong Kong. …”
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PD-1/PD-L1 Axis Downregulation in Guillain-Barré Syndrome: A Potential Mechanism Driving the Autoimmune Pathogenesis by Mr Rasel Ahmed, Mrs Israt Jahan, Mrs Pritha Biswash, Miss Mehjabeen Haque, Miss Sarah Khurshid, Mr Jigishu Ahmed, Mr Sayed Akib Hossain, Dr Zhahirul Islam

“…Mann-Whitney t-tests and simple linear regression were done to compare the PD-1/PD-L1 gene expression and their correlation with T-cell subsets. …”
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SARS-CoV-2 Antibodies in Response to COVID-19 Vaccination in Underserved Racial/Ethnic Minority People Living with HIV by Yongjun Huang, Haley R. Fonseca, Leonardo Acuna, Wensong Wu, Xuexia Wang, Samantha Gonzales, Manuel Barbieri, David R. Brown, Marianna K. Baum

“…Drug use was confirmed via urine toxicology. Multivariable linear regression was conducted. <b>Results</b>: Median age (<i>n</i> = 1317) was 57.8 years, 49.8% were male, 50% were Black non-Hispanic, 66.2% had received ≥1 dose of a COVID-19 vaccine, and 29.6% were PLWH (71.3% virally suppressed and median CD4⁺ T cell count > 500 cells/µL). …”
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Late-gestation heat stress alters placental structure and function in multiparous dairy cows by L.T. Casarotto, H.N. Jones, P. Chavatte-Palmer, J. Laporta, F. Peñagaricano, V. Ouellet, J. Bromfield, G.E. Dahl

“…A representative cotyledon sample was isolated for histological analysis. Tissues were also processed for RNA sequencing and DNA methylation analysis. …”
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SARS-CoV-2 Infections in a Triad of Primary School Learners (Grades 1-7), Their Parents, and Teachers in KwaZulu-Natal, South Africa: Protocol for a Cross-Sectional and Nested Case... by Reshmi Dassaye, Terusha Chetty, Brodie Daniels, Zakir Gaffoor, Elizabeth Spooner, Trisha Ramraj, Ncengani Mthethwa, Duduzile Faith Nsibande, Saresha Pillay, Arvin Bhana, Vuyolwethu Magasana, Tarylee Reddy, Khanya Mohlabi, Penelope Linda Moore, Wendy A Burgers, Tulio de Oliveira, Nokukhanya Msomi, Ameena Goga

“…Statistical methods will include generalized linear mixed models, intracluster correlation, descriptive analysis, and graphical techniques. …”
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Dominio físico de la alfabetización motriz y su relación con el disfrute en clases de Educación Física de estudiantes de 5to y 6to grado (Physical domain of physical literacy and i... by Gustavo Pavez-Adasme, Rubén Chambilla, Cristobal Grez, Jaime Cárcamo-Oyarzún, Felipe Poblete-Valderrama, Juan Párraga-Montilla, Nicolás Gómez-Álvarez

“…Material and Method: This study employed a non-experimental descriptive-correlational design. The sample consisted of 70 students from fifth (n=32) and sixth (n=38) grade. …”
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Metagenomic analysis reveals distinct patterns of gut microbiota features with diversified functions in C. difficile infection (CDI), asymptomatic carriage and non-CDI diarrhea by Lamei Wang, Xinhua Chen, Nira R. Pollock, Javier A. Villafuerte Gálvez, Carolyn D. Alonso, Dangdang Wang, Kaitlyn Daugherty, Hua Xu, Junhu Yao, Yulin Chen, Ciaran P. Kelly, Yangchun Cao

“…Beta diversity for species composition differed significantly between CDI vs Carrier/Control cohorts (p < 0.05). Microbial Linear discriminant analysis Effect Size and ANCOM analysis both identified 8 species (unclassified_f_Enterobacteriaceae, Veillonella_parvula, unclassified_g_Klebsiella and etc.) were enriched in CDI, Enterobacter_aerogenes was enriched in Diarrhea, Collinsella_aerofaciens, Collinsella_sp_4_8_47FAA, Collinsella_tanakaei and Collinsella_sp_CAG_166 were enriched in Control (LDA >3.0, adjusted p < 0.05). …”
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Motivos para practicar actividad física en estudiantes universitarios ecuatorianos (Reasons to practice physical activity in ecuadorian university students) by Borys León-Reyes, León Arguello Nuñez, Yosbanys Roque Herrera, Mauricio Cresp-Barria, Josivaldo Souza-Lima, Dilan Alberto Galeano Rojas

“…The Mann-Whitney U test, Spearman's correlation coefficient and a linear regression analysis were used to analyze the results. …”
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How reliable is the labeling of a commercial phytosteroid product? A 12-week randomized double-blind training study by Joshua Dissemond, Tim Havers, Steffen Held, Stephan Geisler, Tihomir Kostov, Patrick Diel, Svea Türschmann, Maria K. Parr, Eduard Isenmann

“…Data were analyzed using a linear mixed model (LMM).Results Twenty-four participants completed the study successfully. …”
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