Detection of chimeric alpha-defensin transcripts and peptides in mouse Paneth cells by Steven Timmermans, Steven Timmermans, Charlotte Wallaeys, Charlotte Wallaeys, Somara De Beul, Somara De Beul, Natalia Garcia-Gonzales, Natalia Garcia-Gonzales, Claude Libert, Claude Libert

“…IntroductionIn mammals, Paneth cells, located in the crypts of the small intestine, produceantimicrobial peptides that serve to keep the intestinal microbiome under control. a-Defensins are the primary antimicrobial peptides produced by these cells.MethodsWe used 148 publicly available bulk RNA-seq samples on purified PCs, proteomics on enriched purified PC proteins and Defa peptide activity assays to detect all Defa transcrips, including potential chimeric transcrips.ResultsWe identified 28 expressed Defa genes in mice, with up to 85% of Paneth cell RNA reads mapping to these genes. …”
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Design of ELISA-based diagnostic system for detection of enterohaemorrhagic Escherichia coli by Mohammad Javad Rezaei, Maryam Eidi, Seyed Ali Mirhosseini, Rouhollah Kazemi, Mohammad Javad Motamedi, Soghra Khani, Jafar Amani

“…These antigens can be very useful in detecting these bacteria. The aim of this study was to produce recombinant EspA, Intimin and Tir proteins (rEIT) to detect pathogenic E. coli O157:H7 by means of ELISA method. …”
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First Detection of <i>Lactococcus petauri</i> in Domestic Dogs in Italy by Simona Sciuto, Giuseppe Esposito, Paolo Pastorino, Khalid Shahin, Katia Varello, Eliana Trabunella, Giulia Milanese, Sonia Scala, Marino Prearo, Pier Luigi Acutis, Angelo Salerno, Simona Zoppi, Silvia Colussi

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CONCEPTUAL APPROACHES TO THE RAPID DETECTION OF CAMPYLOBACTER SPP. IN MEAT OF SLAUGHTER ANIMALS by D. S. Bataeva, M. Yu. Minaev, Yu. K. Yushina, O. V. Sokolova, E. V. Zajko

“…It was found that the selected primers have 100% convergence to the genome of Campylobacter genus bacteria, the PCR efficiency is not less than 95%, and the detection limit is not more than 1× 104 CFU/g. When estimating the specificity of the primers, it was taken into account that the bacteria of Campylobacter genus may be incorporated in a consortium with intestine microbiome, mainly with Enterobacteriaceae and lactic acid bacteria. …”
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Portulaca oleracea L. (purslane) extract ameliorates intestinal inflammation in diet-induced obese mice by inhibiting the TLR4/NF-κB signaling pathway by Lingchao Miao, Meng Sam Cheong, Haolin Zhang, Haroon Khan, Haroon Khan, Hongxun Tao, Yuxiao Wang, Wai San Cheang

“…Then, intestinal tissues were detached from the mice for detecting protein expressions through Western blot and immunohistochemical analyses. …”
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Observation of intestinal flora diversity with the parasites infection process in a nonlethal malaria model of BALB/c mice induced by Plasmodium yoelii 17XNL strain by Wei Guan, Daoxiu Xu, Shuguo Yang, Yanqing Zhao, Yiting Xie, Min Lin, Yaqun Liu, Yuzhong Zheng, Jian Li

“…In this study, the alteration of intestinal flora composition in BALB/c mice infected with Py 17XNL was detected. …”
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Prevalence of intestinal parasites and associated factors among food handlers in food establishments in the Lideta subcity of Addis Ababa, Ethiopia: an institution-based, cross-sec... by Zemichael Gizaw, Woinishet Abera, Binyam Gintamo, Tewoderos Shitemaw, Zelalem Negash Mekuria

“…Multivariable binary logistic regression was used to identify factors associated with the prevalence of intestinal parasites on the basis of adjusted OR (AOR) and 95% CI and p values &lt;0.05.Setting Food establishments in the Lideta subcity of Addis Ababa, Ethiopia.Participants 411 food handlers participated in the study.Outcome measures The primary outcome was the prevalence of intestinal parasites, defined as the presence of one or more intestinal parasitic species in stool samples.Results One or more intestinal parasites were detected in 171 (41.6%; 95% CI 36.6% to 46.4%) stool samples. …”
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Long-term outcomes of endoscopic resection and tailored adjuvant radiotherapy for sinonasal intestinal-type adenocarcinoma: a historical single-center cohort study in 200 patients by Quentin-Alexandre Parys, Matthias De Witte, Esther Hauben, Paul M. Clement, Robert Hermans, Thomas Decramer, Thomas Decramer, Johannes van Loon, Johannes van Loon, Sandra Nuyts, Sandra Nuyts, Mark Jorissen, Vincent Vander Poorten, Vincent Vander Poorten, Laura Van Gerven, Laura Van Gerven, Laura Van Gerven

“…BackgroundSinonasal intestinal-type adenocarcinoma (ITAC) is a rare disease entity. …”
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Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota by Shulin Wei, Mingxing Li, Long Zhao, Tiangang Wang, Ke Wu, Jiayue Yang, Yubin Liu, Yueshui Zhao, Fukuan Du, Yu Chen, Shuai Deng, Jing Shen, Zhangang Xiao, Wanping Li, Xiaobing Li, Yuhong Sun, Li Gu, Mei Wei, Zhi Li, Xu Wu

“…Intestinal epithelial apoptosis was detected by TUNEL staining. …”
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Biomarkers to predict the severity of acute pancreatitis by Xinwei Hao, Zimeng Wang, Xiaotong Niu, Longsong Li, Yawei Bi, Ningli Chai

“…In this review, we aim to summarize newly discovered biomarkers for the prediction of the severity of acute pancreatitis in the recent years, and provide an overview of serum markers, intestinal flora, and genetic markers. At the same time, the development of emerging detection technologies [e.g., Robust AP Identification and Diagnosis (RAPIDx) and droplet digital PCR (ddPCR)] also provides new possibilities for early prediction of SAP, allowing these biomarkers to be applied clinically.…”
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Performance validation of deep-learning-based approach in stool examination by Kristal Dale Felimon Corpuz, Teera Kusolsuk, Benjamaporn Wongphan, Putza Chonsawat, Kaung Myat Naing, Siridech Boonsang, Veerayuth Kittichai, Chia-Kwung Fan, Santhad Chuwongin, Dorn Watthanakulpanich

“…Abstract Background Human intestinal parasitic infections (IPI) pose a significant global health issue caused by parasitic helminths and protozoa, affecting around 3.5 billion people worldwide, with more than 200,000 deaths annually. …”
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Concomitant Infection of Helicobacter pylori and Intestinal Parasites: Burden, Sociodemographic and Clinical Characteristics in Hospitalized Children and Adolescents in Northern Le... by Ghalia Khoder, Nour Mina, Sara Daher, Sara MINA

“…Conclusion Our data highlighted the association between H. pylori and intestinal parasitic infections. Thus, H. pylori detection could be taken into consideration while screening for parasitic infections in children and adolescents.…”
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Association of Helicobacter pylori vacA polymorphisms with the risk of gastric precancerous lesions in a Moroccan population by Mohamed Reda Jouimyi, Ghizlane Bounder, Imane Essaidi, Hasna Boura, Wafaa Badre, Hakima Benomar, Khalid Zerouali, Halima Lebrazi, Anass Kettani, Fatima Maachi

“…The most common vacA combination was s2/m2/i2/d2 (52%), and it was more detected in chronic gastritis. The moderate virulent vacA combination (s1/m2/i1/d1) increases the risk of atrophic gastritis, while the most virulent vacA combination (s1/m1/i1/d1) increases the risk of intestinal metaplasia. …”
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Exploring the multifaceted challenges of gastrointestinal metastases in lung adenocarcinoma: a case report highlighting diagnostic dilemmas and therapeutic innovations by Xinxin Xu, Qian Zhou, Qian Zhou, Peng Chen, Chengzhou Du, Yonghua Huang, Xiaoxin Gao, Shumei Xu, Jinling Wu, Tianxiao He, Hongtao Li

“…The patient underwent chemotherapy, after which he developed intestinal obstruction. Further imaging and histopathological analysis indicated GI metastasis. …”
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Fecal microbiota transplantation from postmenopausal osteoporosis human donors accelerated bone mass loss in mice by Tinglong Chen, Tinglong Chen, Ning Wang, Ning Wang, Yongqiang Hao, Yongqiang Hao, Lingjie Fu, Lingjie Fu

“…H&amp;E staining of intestinal tissues showed obvious intestinal mucosal injury in mice. …”
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Detecting early gastrointestinal polyps in histology and endoscopy images using deep learning by Intissar Dhrari Hajsalem, Yassine Ben Ayed

“…IntroductionThe GastroIntestinal Cancer (GIC) is one of the most common tumors in terms of deaths and diseases. …”
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High-throughput, pan-leukocyte biomarkers for the detection of inflammation in human breastmilk and stool by M. J. Dunnet, M. J. Dunnet, I. M. Morison, D. M. Bond, T. A. Hore

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Influence of differential source patterns in the detection of signals of disproportionate reporting for PARP inhibitors by Jordi Mestres, Jordi Mestres

“…In contrast, rucaparib and niraparib are most frequently reported by American consumers, and American consumers are the main reporters of events such as insomnia and intestinal obstruction, facilitating their detection as SDR for those two drugs. …”
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Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? by Motlatso T. Hlokwe, Nomawethu S. Masina, Boitumelo Letsoko, Sewellyn C. Davey, Anita L. Michel

“…Overall, the VersaTREK automated liquid culture in combination with IS900 PCR showed a comparable level of detection in tissues (12.6%) as histopathology (13.5%), but the detection rate for faecal samples was lower than for tissues (10.8%). …”
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Magnets ingestion as a rare cause of ileus in adults: A case report by Doklestić Krstina, Lončar Zlatibor, Jovanović Bojan, Veličković Jelena

“…We re-ported a case of a man, age 21, who swallowed 2 very small magnets, presented with clinical and radiographic signs of acute bowel obstruction and intestinal fistula. The cause of obstruction was detected during laparotomy. …”
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