Papain-like cysteine proteases in Nicotiana benthamiana: gene family members and their potential implications in recombinant protein expression by Yalun Yang, Yalun Yang, Yalun Yang, Zhichao Deng, Zhichao Deng, Zhongqi Zhang, Yong Yang, Xiaolu Pan, Xiaolu Pan, Rongrong Wu, Rongrong Wu, Tao Liu, Tao Liu, Xiaoming Gao, Xiaoming Gao, Lingyan Li, Yongfeng Guo, Yongfeng Guo

“…This study aims to identify NbPLCP genes in N. benthamiana and elucidate their roles in facilitating heterologous protein expression.MethodsA comprehensive bioinformatics approach was employed to identify NbPLCP genes using N. benthamiana genomic data and homology with Arabidopsis thaliana. The analysis included examination of physicochemical properties, phylogenetic relationships, gene structures, conserved motifs, expression profiles, gene duplication events, and chromosomal distribution. …”
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The OsZHD1 and OsZHD2, Two Zinc Finger Homeobox Transcription Factor, Redundantly Control Grain Size by Influencing Cell Proliferation in Rice by Mingliang Guo, Chun Zheng, Chao Shi, Xiaozhuan Lu, Zeyuan She, Shuyu Jiang, Dagang Tian, Yuan Qin

“…Abstract Grain size is vital determinant for grain yield and quality, which specified by its three-dimensional structure of seeds (length, width and thickness). The ZINC FINGER-HOMEODOMAIN (ZHD) proteins play critical roles in plant growth and development. …”
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Shifts in the rhizosphere microbiome and exudation profile of avocado (Persea americana Mill.) during infection by Phytophthora cinnamomi and in presence of a biocontrol bacterial... by Frédérique Reverchon, Mariel García-Meléndez, Edgar Guevara-Avendaño, Oscar Mora-Chávez, Itzel A. Solís-García, Wesley Dáttilo, José A. Guererro-Analco, Alfonso Méndez-Bravo, Juan L. Monribot-Villanueva, Violeta Patiño-Conde, Fernando Pineda-García, Eria A. Rebollar

“…Conclusions Collectively, our data elucidate the shifts in the avocado rhizosphere microbiome after infection by P. cinnamomi and when inoculated with a BCA, and help understand how a pathogen or a beneficial bacterium can alter plant-microbiome crosstalk. …”
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Effector Pt31812 from Puccinia triticina acts as avirulence factor for Lr42-mediated resistance in wheat by Jianyuan Li, Jianyuan Li, Jiali Li, Jie Wei, Jie Wei, Lin Li, Yue Qi, Yue Qi, Yue Zhang, Yue Zhang, Wenxiang Yang, Qian-Hua Shen

“…In the study, Pt31812 was cloned by PCR, and the expression pattern and structure were analyzed by qRT-PCR and online softwares. …”
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Monoterpene Hydroxy Lactones Isolated from <i>Thalassiosira</i> sp. Microalga and Their Antibacterial and Antioxidant Activities by Alcina M. M. B. Morais, Decha Kumla, Valter F. R. Martins, Ana Alves, Luis Gales, Artur M. S. Silva, Paulo M. Costa, Sharad Mistry, Anake Kijjoa, Rui M. S. C. Morais

“…Two monoterpenoid lactones, loliolide (<b>1</b>) and <i>epi</i>-loliolide (<b>2</b>), were isolated from the crude dichloromethane extract of a microalga, <i>Thalassiosira</i> sp.). The structures of loliolide (<b>1</b>) and <i>epi</i>-loliolide (<b>2</b>) were elucidated by 1D and 2D NMR analysis, as well as a comparison of their ¹H or/and ¹³C NMR data with those reported in the literature. …”
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Serological evaluation of Crimean-Congo Hemorrhagic fever in humans with High-Risk professional exposure and in residual sera collected in 2022-2023 across Corsica (France) by Paloma Kiwan, Morena Gasparine, Dorine Decarreaux, Lisandru Capai, Shirley Masse, Miša Korva, Tatjana Avšič-Županc, Jean Canarelli, Marie-Helene Simeoni, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi

“…Sociodemographic and epidemiological data were collected using a structured questionnaire in the high-risk group. …”
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Risk Factors and Prognostic Implications of Tumor‐Related Epilepsy in Diffuse Glioma Patients: A Real‐World Multicenter Study by Yao Xiao, Zhuang Nie, Jinsha Huang, Jie Zhao, Chengjun Dong, Yan Zou, Zikai Li, Bingqing Yan, Yue Hu, Fan Yang, Jong Woo Lee, Alexander P. Lin, Steven Tobochnik, Min Zhou, Ziqiao Lei

“…This study aimed to assess the risk factors and prognostic impact of TRE in adult patients with diffuse gliomas by integrating clinical, radiological, and molecular data. Methods: This multicenter retrospective study included 1036 adult patients with diffuse gliomas from local hospitals and the POLA Network. …”
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Do neonatal intensive care unit (NICU) health workers know about retinopathy of prematurity (ROP)? A qualitative study at a Regional Referral Hospital in Uganda by Henry Muwonge, Innocent Ayesiga, Primrose Magala, Tom Didimus Ediamu, Hana El Diwany, Ian LY Yeung, Alex Wasomoka, Phillip Musoke, Elias Musiime, Abraham Tumwesigye, Pius Atwau, Noreen Atwijukire, Iddi Ndyabawe, Anne Mpaire Musika, Himanshu I Patel, Aeesha NJ Malik, Ibrahim Bwaga, Sheba Gitta Nakacubo

“…We recommend developing a formal training structure and syllabus for ROP screening. The training can enhance task shifting among health workers in areas without specialised health workers. …”
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A 1.8 m Class Pathfinder Raman LIDAR for the Northern Site of the Cherenkov Telescope Array Observatory—Performance by Pedro José Bauzá-Ruiz, Oscar Blanch, Paolo G. Calisse, Anna Campoy-Ordaz, Sidika Merve Çolak, Michele Doro, Lluis Font, Markus Gaug, Roger Grau, Darko Kolar, Camilla Maggio, Manel Martinez, Samo Stanič, Santiago Ubach, Marko Zavrtanik, Miha Živec

“…Several moderate hardware improvements are planned for its final upgraded version, such as gated PMTs for the elastic channels and a reduced-power laser with a higher repetition rate, to ensure that the data acquisition system is not saturated and therefore not affected by residual ringing.…”
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Butyrate alleviates food allergy by improving intestinal barrier integrity through suppressing oxidative stress‐mediated Notch signaling by Jialu Shi, Wenjun Mao, Yuqing Song, Yuxin Wang, Lili Zhang, Yan Xu, Huiwen Gu, Siyu Yao, Yuanhang Yao, Zhifeng Liu, Vijaya Raghavan, Jin Wang

“…However, butyrate counteracted this loop by restoring microbiota structure and suppressing reactive oxygen species (ROS)/Notch cascades. …”
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Identification, Characterization, Expression Profiling and Functional Analysis of Tobacco <i>CalS</i> Gene Family by Hong Wang, He Meng, Xiaohan Qi, Yi Pan, Bailu Ji, Liuying Wen, Yanjun Zan, Huan Si, Yuanying Wang, Dan Liu, Aiguo Yang, Zhengwen Liu, Lirui Cheng

“…A comprehensive analysis of their physicochemical properties, gene structure, and evolutionary history highlighted their evolutionary conservation. …”
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Lupus nephritis and U1-RNP-antibodies are associated with low bone mineral density and osteoporosis in patients with systemic lupus erythematosus: baseline findings in a sub-cohort... by Edgar Wiebe, Elisa Celine Schilling, Dörte Huscher, Andriko Palmowski, Zhivana Boyadzhieva, Sandra Hermann, Burkhard Muche, Mirella Lopez Picazo, Gerhard Krönke, Falk Hiepe, Tobias Alexander, Frank Buttgereit

“…Methods We analyzed baseline data of a consecutive subcohort of patients with SLE with current or past GC treatment, fulfilling the EULAR/ACR 2019 SLE classification criteria. …”
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Perceived effectiveness and recommendations from a childbirth quality assurance and improvement programme in India’s private sector: a qualitative evaluation using the RE-AIM frame... by Katherine Semrau, Meghna Desai, Lauren Spigel, Somesh Kumar, Megan Marx Delaney, Lauren Bobanski, Suranjeen Pallipamula, Natalie Henrich, Tapas Sadasivan Nair, Hema Divakar, Rajat Chabba, Shweta Jindal, Gulnoza Usmanova, Sukanya Dutta, Aarushi Gupta, Sucheta Kinjawadekar, Priti Kumar, Priyanka Kumari, Prerna Mukharya, Hrishikesh Pai, Ameya Purandare, Pompy Sridhar

“…Participants included facility owners, nurses, FOGSI quality assessors, programme implementers and Manyata leaders. Data were coded and analysed using a deductive and inductive process. …”
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COVID-19 vaccine uptake, barriers and associated factors among healthcare workers in Malawi by Clara Sambani, Tonny Muwonge, Liness Chinyamunyamu, Amon Dembo, Mosoka P. Fallah, Victor Chikwapulo, Mavuto Thomas, Charity Gondwe, Regina Mankhamba, Leah Mbabazi, Suzan Nakasendwa, Rodgers R. Ayebare, Collins Mitambo, Matthew Kagoli, Dzinkambani Kambalame, Clement Seven, Tadala Mwale, Edna Mandala, Abigail Kazembe, McWilliam Kalua, Senga Sembuche, Elizabeth Gonese, Tamrat Shaweno, Nebiyu Dereje, Evelyn C. Banda, Tajudeen Raji, Mitch Matoga

“…Data were managed in REDCap and analysed using STATA version 14. …”
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Clinical effectiveness of mindfulness-based music therapy on improving emotional regulation in blind older women: A randomized controlled trial by Sunny H.W. Chan, Meryl Y.C. Cheung, Armstrong T.S. Chiu, Mimi H.T. Leung, Michael C.C. Kuo, Donald Y.C. Yip, Carole C.Y. Hui, Sally W.I. Kam, King Yeung, Doreen S.P. Mui, Shu-Mei Wang, Calvin C.K. Yip

“…Outcome assessors were blinded to group assignment. Results: Data was analyzed based on intention-to-treat basis. …”
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Identifying the Components of Iranian Adolescent Compassionate Mind Training Program by Somayeh Mohebi, Iraj Shakerinia, Mahnaz Khosrojavid, Abbas Abolghasemi, Abbas Ali Hossein Khanzadeh:

“…Participants included university professors and school counsellors with extensive experience in relevant fields. Data was collected through semi-structured interviews, focusing on the essential components of compassion training for adolescents, distinguishing features of Iranian adolescents, and additional elements needed for cultural adaptation. …”
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Lessons from the PROTECT-CH COVID-19 platform trial in care homes by Philip M Bath, Jonathan Ball, Matthew Boyd, Heather Gage, Matthew Glover, Maureen Godfrey, Bruce Guthrie, Jonathan Hewitt, Robert Howard, Thomas Jaki, Edmund Juszczak, Daniel Lasserson, Paul Leighton, Val Leyland, Wei Shen Lim, Pip Logan, Garry Meakin, Alan Montgomery, Reuben Ogollah, Peter Passmore, Philip Quinlan, Caroline Rick, Simon Royal, Susan D Shenkin, Clare Upton, Adam L Gordon

“…Generic challenges included working across the four home nations with their different structures and regulations. Limitations The feasibility of contracting between the sponsor and the principal investigators, general practitioners and care homes; screening, consent and treatment of care home residents; data acquisition and the potential benefit of postexposure prophylaxis were never tested. …”
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Three-Dimensional Curved Workflow-Based Optical Coherence Tomography Angiography for Enhancing Atopic Dermatitis Theranostics by Junwei Li, Yunrui Zhang, Ying Huang, Ronghui Li, Kun Wang, Dongbei Guo, Zicheng Huang, Youliang Yao, Yunxin Xue, Guibo Sun, Cheng Jiang, Leyun Wang, Chenzhong Li, Qingliang Zhao

“…Optical coherence tomography angiography (OCTA) is a major advancement in imaging, offering high-resolution microvascular volumetric images crucial for diagnosing and studying dermatological diseases. However, current data analysis and clinical evaluation criteria primarily rely on 2-dimensional (2D) imaging results, resulting in imprecise diagnoses due to the substantial loss of 3D curved structures and microvascular details. …”
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Genetic Diversity and Construction of Salt-Tolerant Core Germplasm in Maize (<i>Zea mays</i> L.) Based on Phenotypic Traits and SNP Markers by Yongfeng Song, Jiahao Wang, Yingwen Ma, Jiaxin Wang, Liangliang Bao, Dequan Sun, Hong Lin, Jinsheng Fan, Yu Zhou, Xing Zeng, Zhenhua Wang, Lin Zhang, Chunxiang Li, Hong Di

“…A core collection of 172 accessions was selected based on both phenotypic and genotypic data. These were further evaluated for salt–alkali tolerance during germination, and cluster analysis classified them into five groups. …”
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Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China by Yongqiang Yang, Yanxian Yang, Guanping Chen, Minmin Lin, Yuan Chen, Ruowen He, Klibs N. Galvão, Mohamed Abd El-Gawad El-Sayed Ahmed, Adam P. Roberts, Yiping Wu, Lan-Lan Zhong, Xiaoxue Liang, Mingyang Qin, Xin Ding, Wenbin Deng, Songyin Huang, Hong-Yu Li, Min Dai, Ding-Qiang Chen, Liyan Zhang, Kang Liao, Yong Xia, Guo-Bao Tian

“…Integrated epidemiological and genomic data showed that ST11 and ST307 Kp isolates were associated with nosocomial outbreak and transmission. …”
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