Accurate and rapid single nucleotide variation detection in PCSK9 gene using nanopore sequencing by Ilaria Massaiu, Vincenza Valerio, Valentina Rusconi, Valentina Rusconi, Francesca Bertolini, Donato De Giorgi, Veronika A. Myasoedova, Paolo Poggio, Paolo Poggio

“…We tested different nanopore sequencing pipelines aimed at accurately detecting single-nucleotide variants (SNV) in a gene locus spanning ⁓25 kb.MethodsAs a proof of concept, PCSK9 was selected for its relevance to cardiovascular disease and suitable sequence structure. Twelve subjects were analyzed using different sequencing flow cells, basecalling models, and SNV calling algorithms. …”
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Research Progress on Tomato Leaf Color Mutants by Yingxue HU, Weijie XU, Chao GONG, Baojuan SUN, Zhou HENG, Yuyang ZHANG, Tao LI

“…In order to gain a deeper understanding of the photosynthetic system structure, functional genomics, and genetic breeding of tomatoes, this article focuses on exploring the formation mechanism of tomato leaf color variation and the identification of key genes. …”
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Clinical Efficacy of Endocannabinoid-Mimetic Fatty Acid Amide as a Skin-Soothing Ingredient by Sungwoo Kim, Yeonjae Kim, Juyeon Jung, Hyun-Jung Kim, Sekyoo Jeong, Heesung Shin, Wuk-Sang Ryu, Jung-Hoon Sohn, Gaewon Nam

“…To develop an alternative to cannabidiol, fatty acid amide molecules mimicking the chemical structure of endocannabinoids were manufactured using a lipase-catalyzed process. …”
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Natural fish swim bladder-derived MPN-nanofibrous biomimetic system exhibit ECM-responsive signal regulation and promote robust tendon–bone healing by Lei Shi, Peng Zhou, Cong Ye, Jie Sun, Hongdong Ma, Ran Tao, Peng Zhang, Fei Han

“…Inspired by the composition and aligned nanofibrous structure of the natural fish bladder matrix (fish swim bladder, FSB), we employed a gallium (Ga)‒tannic acid (TA) metal‒polyphenol network (MPN)-modified decellularized fish bladder matrix (GaPP@FSB) as a novel biomaterial to address this problem. …”
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Xylooligosaccharides from Barley Malt Residue Produced by Microwave-Assisted Enzymatic Hydrolysis and Their Potential Uses as Prebiotics by Shah Zaib Fareed, Pipat Tangjaidee, Tabkrich Khumsap, Wannaporn Klangpetch, Suphat Phongthai, Apinun Kanpiengjai, Chartchai Khanongnuch, Kridsada Unban

“…Thin-Layer Chromatography (TLC) and high-performance liquid chromatography (HPLC) clearly elucidated the oligosaccharide compositions, and the result of Fourier Transform Infrared Spectroscopy (FTIR) confirms the molecular structure and sugar components of achieved BMR-XOS. …”
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Non-targeted screening and toxicity study of safety risk substances in facial skincare products: Molecular networking and computational toxicology strategy by Dong Guo, Yaxiong Liu, Jingwen Liang, Yayang Huang, Yangjie Li, Jihui Fang, Sheng Yin

“…Methods: Molecular networking was utilized for the non-targeted screening of risk substances in facial skincare products, and the toxicity of these risk substances was evaluated through molecular docking and quantitative structure-activity relationship (QSAR) models. Results: Through molecular networking, we identified seven known prohibited ingredients, six of which were confirmed using standard substances. …”
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Thermal analysis and experimental design of PVDF-trfe-based pyroelectric sensors for infrared applications by Mohamed Behloul, Essaid Bousbiat, Dalila Yamani, Riyadh Bouddou, Nour Eddine Derguini, Ayodeji Olalekan Salau, Djamel Kendil, Sarra Adiche

“…The sensor architecture comprises a multilayer structure consisting of a PVDF-TrFE active layer, a 2 μm air gap for thermal insulation, and a silicon substrate. …”
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Tyrosine phosphatase Shp2 accelerated the progression of dilated cardiomyopathy via upregulating NF-κB/Src/FAK signaling induced ROS and mitophagy by Jingxia Hao, Hua Wang, Bo Li, Jingshi Chen, Wenlu Wang, Yingxue Wang, Yingqian Zhang

“…Dilated cardiomyopathy was ameliorated in DCM mice with specific knockout of Shp2, which showed attenuation of heart structure, degree of fibrosis in myocardial tissue, and degree of cardiomyocyte dilatation. …”
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Autonomous slip control inspired by human physiology for improved shared control strategy by Joana Matos, Patricia Capsi-Morales, Cristina Piazza

“…The human hand is an intricate anatomical structure essential for daily activities, yet replicating its full functionality in upper-limb prostheses remains a significant challenge. …”
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Quantum Vulnerability Analysis to Guide Robust Quantum Computing System Design by Fang Qi, Kaitlin N. Smith, Travis LeCompte, Nian-feng Tzeng, Xu Yuan, Frederic T. Chong, Lu Peng

“…The ESP suffers poor prediction since it fails to account for the unique combination of circuit structure, quantum state, and quantum computer properties specific to each program execution. …”
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Effect of WSSV infection on immunity, intestinal microbial composition and its derived metabolites synthesis in Procambarus clarkii by Xiaochuan Zheng, Jie Yang, Feifan Xu, Qunlan Zhou, Cunxin Sun, Aimin Wang, Jinjuan Wan, Jiajia Li, Bo liu

“…We constructed an artificial infection model of WSSV in crayfish and conducted a comprehensive investigation into the alterations in non-specific immunity, intestinal microbial structure, and functional metabolites of crayfish following WSSV infection by integrating 16S rDNA and targeted metabolomics techniques. …”
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A comparative analysis of thermal comfort, energy consumption, pollutant emissions, and visual comfort in a school building utilizing electrochromic and thermochromic windows by Sina Lashgari, Mohammad Hossein Jahangir

“…This study investigates a school building, a structure particularly sensitive to thermal and visual comfort, simulated across three cities in Iran, each characterized by distinct climatic conditions. …”
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Acceleration and Transport of the Unstable Cosmic-Ray Isotope 60Fe in Supernova-enriched Environments by Xin-Yue Shi, Martin Pohl, Michael M. Schulreich

“…The main uncertainty in our prediction of the local ^60 Fe flux at about pc = 1 GeV nuc ^−1 is the magnetic-field structure in the Local Bubble and the cosmic-ray diffusion beyond the approximately 100 kyr of evolution covered by our study. …”
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FsDAOD: Few-shot domain adaptation object detection for heterogeneous SAR image by Siyuan Zhao, Yong Kang, Hang Yuan, Guan Wang, Hui Wang, Shichao Xiong, Ying Luo

“…Firstly, employing the foundational structure of the existing baseline method, a novel mutual information loss function is introduced that prompts the neural network to extract domain-specific knowledge. …”
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Targeted dual-receptor phage cocktail against Cronobacter sakazakii: insights into phage-host interactions and resistance mechanisms by Seongok Kim, Bokyung Son, Yeran Kim, Yeran Kim, Hyeongsoon Kim, Gahyeon Nam, Hakdong Shin, Sangryeol Ryu, Sangryeol Ryu, Sangryeol Ryu

“…Phenotypic analysis of the phage cocktail resistant strain, designated as CSR strain, revealed that the resistance resulted from the impaired receptor proteins for phage, such as defects in motility and alteration in LPS structure. CSR strain exhibited significant attenuation in invading human intestinal epithelial Caco-2 cells compared to WT cells.ConclusionThis study demonstrates that the development of the phage cocktail targeting distinct host receptors can serve as a promising antimicrobial strategy to effectively control C. sakazakii.…”
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Polarity‐Reversal of Exchange Bias in van der Waals FePS3/Fe3GaTe2 Heterostructures by Han Xiao, Bingbing Lyu, Mengjuan Mi, Jian Yuan, Xiandong Zhang, Lixuan Yu, Qihui Cui, Chaofan Wang, Jun Song, Mingyuan Huang, Yufeng Tian, Liang Liu, Takashi Taniguchi, Kenji Watanabe, Min Liu, Yanfeng Guo, Shanpeng Wang, Yilin Wang

“…., μ0H ≥ 0.5 T), a negative EB field (HEB) is observed at low temperatures, and with increasing temperature, the HEB crosses zero at ≈20 K, subsequently becomes positive and later approaches zero again at Tb. A model composed of a top FePS3/interfacial FePS3/Fe3GaTe2 sandwich structure is proposed. …”
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Microfluidics-enabled polydopamine-coated selenium nanoparticles in hyaluronic hydrogel microspheres for targeted antioxidant and immunomodulatory therapy of ulcerative colitis by Ying Fang, Hongwei Yu, Xiaochen Feng, Haike Xia, Yunze Xu, Ranjith Kumar Kankala, Aizheng Chen, Chaoping Fu

“…The uniform microspheres featured a microfluidics-enabled structure that enabled stable CS-Se@PDA nanoparticles anchoring via electrostatic and covalent interactions. …”
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Psychometric properties of the Turkish version of the respectful maternity care scale https://nmcjournal.zums.ac.ir/article-1-935-en.html by Sule Gokyildiz Surucu, Gunes Arslan, Burcu Avcibay Vurgec, Ebru Gozuyesil, Ephrem Daniel Sheferaw

“…An analysis of the new results indicated a three-factor structure with a Kaiser-Meyer-Olkin (KMO) value of .84 explaining 70.74% of the total variance. …”
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An integrated empirical and computational study to decipher help-seeking behaviors and vocal stigma by Aaron R. Glick, Colin Jones, Lisa Martignetti, Lisa Blanchette, Theresa Tova, Allen Henderson, Marc D. Pell, Nicole Y. K. Li-Jessen

“…Network analysis was performed to evaluate the effect of social network structure on the flow of IMB among virtual agents. …”
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Hypoxia induces robust ATP release from erythrocytes in ApoE-LDLR double-deficient mice by Fatih Celal Alcicek, Fatih Celal Alcicek, Jakub Dybas, Katarzyna Bulat, Tasnim Mohaissen, Tasnim Mohaissen, Ewa Szczesny-Malysiak, Magdalena Franczyk-Zarow, Katarzyna M. Marzec

“…This supports our previous reports on the role of the protein structure alterations of RBC cytosol in hypoxia-induced ATP release from RBCs in ApoE/LDLR−/− mice. …”
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