Sources of uncertainty in DNA metabarcoding of whole communities: Implications for its use in biomonitoring by J. Iwan Jones, Amanda Arnold, Dominik Buchner, John F. Murphy, Jasmine N. Childress, Pedro Beja, Florian Altermatt, Kristian Meissner, Ian Adams, Meri Anderson, Yaisel J. Borrell, Andrew G. Briscoe, Kat Bruce, Cátia Chaves, Chris Conyers, Kristy Deiner, Vasco Elbrecht, Álvaro Fueyo, Tim Goodall, Javier Granero‐Castro, Cristina Hartmann‐Fatu, Eleanor Jones, Elvira Mächler, Filipa M. S. Martins, Sam McGreig, Lyndall Pereira‐da‐Conceicoa, James L. Pretty, Daniel S. Read, Ben Price, Florian Leese, Rosetta C. Blackman

“…However, the routine application of DNA‐based methods to monitor whole communities using a metabarcoding approach and derive ecosystem status continues to be limited. We undertook a structured experiment to assess factors influencing the precision and accuracy of molecular methods for freshwater macroinvertebrate community assessment. …”
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Observational Comparison between Confined and Eruptive Flares: Magnetohydrodynamics Instability Parameters in a Similar Magnetic Configuration by Kouhei Teraoka, Daiki Yamasaki, Yusuke Kawabata, Shinsuke Imada, Toshifumi Shimizu

“…Unstable states of the solar coronal magnetic field structure result in various flare behaviors. In this study, we compared the confined and eruptive flares that occurred under similar magnetic circumstances in the active region 12673, on 2017 September 6, using the twist number, decay index, and height of magnetic field lines to identify observational behaviors of the flare eruption. …”
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Cluster-Based Flight Path Construction for Drone-Assisted Pear Pollination Using RGB-D Image Processing by Arata Kuwahara, Tomotaka Kimura, Sota Okubo, Rion Yoshioka, Keita Endo, Hiroyuki Shimizu, Tomohito Shimada, Chisa Suzuki, Yoshihiro Takemura, Takefumi Hiraguri

“…Flower detection is performed using a YOLO (You Only Look Once)-based object detection algorithm, and three-dimensional flower positions are estimated by integrating depth information with the drone’s positional and orientation data in the east-north-up coordinate system. To enhance pollination efficiency, the method applies the OPTICS (Ordering Points To Identify the Clustering Structure) algorithm to group detected flowers based on spatial proximity that correspond to branch-level distributions. …”
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Ante-Mortem Clinical Characterization with Post-Mortem Family Interview and Medical Record Abstraction in a Traumatic Brain Injury Brain Donor Program by Amelia J. Hicks, Enna Selmanovic, Ariel Pruyser, Carley R. Trentman, Joshua C. Klein, Kaitlyn Wilkey, Miguel X. Escalon, Natalia Bernal-Fernández, Belinda Yew, Kristen Dams-O’Connor

“…We describe the content development and structure of the LETBI PFI; interviewer training and qualifications; and considerations regarding interview duration, informant selection, and interview timing, as well as PFI inter-rater reliability. …”
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Molecular epidemiology of Ascaris lumbricoides following multiple rounds of community-wide treatment by Toby Landeryou, Rosie Maddren, Jack Hearn, Mahlet Belachew, Santiago Rayment Gomez, Ewnetu Firdawek Liyew, Kathryn Forbes, Birhan Mengistu, Scott P. Lawton, Jude Eze, Geremew Tasew, Ufaysa Angulo, Roy Anderson

“…We found that fine-scale population structure exists in spatially distinct clusters of infected individuals with reinfection occurring within or between geographically close households. …”
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Aberrant fragmentomic features of circulating cell-free mitochondrial DNA as novel biomarkers for multi-cancer detection by Yang Liu, Fan Peng, Siyuan Wang, Huanmin Jiao, Miao Dang, Kaixiang Zhou, Wenjie Guo, Shanshan Guo, Huanqin Zhang, Wenjie Song, Jinliang Xing

“…Furthermore, region-specific fragmentomic features of ccf-mtDNA were observed, which was associated with protein binding, base composition and special structure of mitochondrial DNA. When comparing to non-cancer controls, six types of cancer patients exhibited aberrant fragmentomic features. …”
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Impact of Anthropomorphic Shape and Skin Stratification on Absorbed Power Density in mmWaves Exposure Scenarios by Silvia Gallucci, Martina Benini, Marta Bonato, Valentina Galletta, Emma Chiaramello, Serena Fiocchi, Gabriella Tognola, Marta Parazzini

“…As data exchange demands increase also in widespread wearable technologies, transitioning to higher bandwidths and mmWave frequencies (30–300 GHz) is essential. …”
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First Assembly of a Draft Genome of the Critically Endangered Northern Muriqui (Brachyteles hypoxanthus, Primates, Atelidae) Including Non‐Invasive Genotyping Strategies for the Sp... by Amanda Alves de Melo‐Ximenes, Romina Batista, Leonardo Carlos Jeronimo Corvalán, Tomas Marques‐Bonet, Lukas Kuderna, Kyle Farh, Jeffrey Rogers, Mariane da Cruz Kaizer, Jean Philippe Boubli, Fabiano Rodrigues de Melo, Rhewter Nunes, Mariana Pires de Campos Telles

“…The complete 16,635 bp mitochondrial genome retains the conserved mammalian structure with 22 tRNAs, 2 rRNAs, 13 CDS, and an origin of replication. …”
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EVALUATION OF RATIONALITY OF EXPENSES ON TREATMENT OF SEVERE COMMUNITY-ACQUIRES PNEUMONIA IN MULTI-PROFILE HOSPITAL by I. V. Demko, N. V. Gordeeva, E. N. Bochanova, N. I. Golovina, A. Yu. Kraposhina, I. A. Soloveva, E. A. Sobko, T. V. Gaygolnik, S. V. Chubarova

“…The frequency analysis was applied to studying the structure of the carried-out drug treatment and identification of frequency of the separate medicinal preparations. …”
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Analysis of the Influence of Different Turbulence Models on the Prediction of Vehicle Aerodynamic Performance by Luwei Wang, Xingjun Hu, Peng Guo, Zirui Wang, Jingyu Wang, Yuqi Wang, Yan Ma, Ying Li, Jing Zhao, Xu Yang, Ruixing Ma, Yinan Zhu, Jianjiao Deng

“…These models predict vehicle aerodynamic resistance, lift, and wake flow structure. The k-ε model best predicts the steady-state drag coefficient (Cd) (error 0.0009). …”
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Physiologically based pharmacokinetic model of sodium-glucose cotransporter 2 inhibitors predicted pharmacokinetics and pharmacodynamics to explore dosage regimen for patients with... by Guimu Guo, Guimu Guo, Meng Ke, Meng Ke, Jianwen Xu, Jianwen Xu, Wanhong Wu, Wanhong Wu, Jiarui Chen, Jiarui Chen, Chengjie Ke, Chengjie Ke, Pinfang Huang, Pinfang Huang, Cuihong Lin, Cuihong Lin

“…ObjectiveThis study aimed to compare the hypoglycemic effects of four SGLT2 inhibitors (dapagliflozin, canagliflozin, empagliflozin, and ipragliflozin), simulate the 24-h urinary glucose excretion (UGE) of these inhibitors in T2DM patients with renal insufficiency, and investigate optimal dosage regimen for the SGLT2 inhibitor in these patients.MethodWe established a physiologically based pharmacokinetic (PBPK) model of SGLT2 inhibitors using the PK-Sim software, and the renal physiological tissue structure was expanded to include renal tubules using the MoBi software. …”
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Evaluating analytic strategies to obtain high-resolution, vertex-level measures of cortical neuroanatomy in children in low- and middle-income countries by Charlotte M. Pretzsch, František Váša, Michael Brammer, Lucy Brink, Mandy Potter, Wendy Mackay, Petrusa Smit, Carolina Du Plessis, Marcelle Wagner, Deborah Jonker, Kirsten A. Donald, Freda Scheffler, Cindy Pham, Viola Hollestein, Eva Loth, Declan GM Murphy, Hein J. Odendaal, Elizabeth R. Sowell, Priscilla E. Springer, Dan J. Stein, Christine Ecker

“…Abstract High-field magnetic resonance imaging to explore brain structure and function remains limited to high-resource settings. …”
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Optical Coherence Tomography Angiography, Elastography, and Attenuation Imaging for Evaluation of Liver Regeneration by Svetlana Rodimova, Ekaterina Gubarkova, Nikolai Bobrov, Ilya Shchechkin, Vera Kozlova, Natalia Zolotova, Arseniy Potapov, Elena Kiseleva, Grigory Gelikonov, Natalia Gladkova, Vladimir Zagainov, Elena Zagaynova, Daria Kuznetsova

“…In this study, we used multimodal optical coherence tomography (MM OCT), a combination of three optical coherence tomography modalities—OCT–angiography (OCTA), attenuation coefficient mapping, and OCT–elastography (OCE) to provide real-time three-dimensional and label-free assessment of changes in microcirculation, and in the structure and stiffness of the liver during regeneration. …”
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Quantifying of spatio-temporal variations in the regional gravity field and the effectiveness of earthquake prediction: A case study of MS ≥ 5.0 earthquakes in the Sichuan-Yunnan r... by Weimin Xu, Shi Chen, Yongbo Li, Jiangpei Huang, Bing Zheng, Yufei Han, Zhaohui Chen, Qiuyue Zheng, Hongyan Lu, Linhai Wang, Honglei Li, Dong Liu

“…Reportedly, crustal mass transfer, which is affected by stress state and structural environment, alters the characteristics of the regional gravity field, thus serving as an indicator for locations of moderate to strong earthquakes and a seismology-independent predictor for regions at risk for strong earthquakes. …”
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Rivastigmine interferes with the pharmacological activity of hydromethylthionine on presynaptic proteins in the line 66 model of frontotemporal dementia by Karima Schwab, Lianne Robinson, Anne Annschuetz, Eline Dreesen, Mandy Magbagbeolu, Valeria Melis, Franz Theuring, Charles R. Harrington, Claude M. Wischik, Gernot Riedel

“…We measured the abundance of tau and synaptic proteins (VAMP-2, SNAP-25, SNTX-1, SYNPY-1, SYN-1, A-SYN) immunohistochemically to reveal structural synaptic alterations in these mice. Tau and synaptic markers were also examined in L66 mice treated with hydromethylthionine mesylate (HMTM) (15 mg/kg) and rivastigmine (0.5 mg/kg) administered singly and in combination. …”
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Comparative analysis of corpus callosum lipidome and transcriptome in schizophrenia and healthy brain by Maria S. Osetrova, Olga I. Efimova, Marina D. Zavolskova, Elena A. Stekolschikova, Gleb N. Vladimirov, Dmitry A. Senko, Tatiana A. Zhuravleva, Anna Y. Morozova, Yana A. Zorkina, Denis S. Andreyuk, George P. Kostyuk, Evgeniy N. Nikolaev, Philipp E. Khaitovich

“…BACKGROUND: Functional and structural studies of the brain highlight the importance of white matter alterations in schizophrenia. …”
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Knowledge, attitudes and practices regarding pulmonary rehabilitation among patients with chronic respiratory diseases: a cross-sectional questionnaire-based study in a tertiary ho... by Xueli Bai, Yuan Chen, Xiansheng Liu, Lingling Wang, Xiaohong Zhang, Jie Ding, Lin Su, Ruiying Wang, Shuang Wei

“…The secondary outcome assessed the association between demographic factors (age, gender, income, education, smoking status) and KAP scores using structural equation modelling (SEM).Results The mean KAP scores were 1.26±2.85 for knowledge, 30.46±3.63 for attitudes and 34.58±5.55 for practices. …”
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Innovative strategies for bone organoid: Synergistic application and exploration of advanced technologies by Xu Lou, Qirong Zhou, Zhenglin Dong, Long Bai, Jiacan Su, Hua Yue

“…While 3D bioprinting enhances spatial precision and structural complexity, artificial intelligence accelerates organoid optimization through data-driven approaches. …”
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Unique epitope–antibody interactions in the intrinsically disordered proteoglycan-like domain of human carbonic anhydrase IX defined by high-resolution NMR combined with yeast surf... by Feng Ni, Cunle Wu, Ping Xu, Ping Wang, Yves Fortin, Melanie Arbour, Luke Masson, Denis L’Abbé, Andrea Acel, Mylene Gosselin, Anne E.G. Lenferink

“…Transverse relaxation optimized NMR spectroscopy of 11H9-F(Ab) in complex with the CA-IX IDR outlines structural rigidification of a linear epitope, while the rest of the IDR remains largely unstructured upon complex formation. …”
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A Multidata Approach to Open Clusters: Roslund 3 and Ruprecht 174 in CCD UBV and Gaia DR3 Context by Hülya Karagöz, Talar Yontan, Selçuk Bilir, Olcay Plevne, Tansel Ak, Serap Ak, Remziye Canbay, Timothy Banks

“…A detailed analysis of the structural, astrophysical, kinematic, and dynamical properties of the open clusters Roslund 3 and Ruprecht 174 is carried out using CCD UBV photometry in conjunction with astrometric and photometric data from Gaia DR3. …”
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