Germline variants in patients from the Iranian hereditary colorectal cancer registry by Lena Goshayeshi, Saeed Hoorang, Benyamin Hoseini, Mohammad Reza Abbaszadegan, Maryam Afrazeh, Maliheh Alimardani, Fatemeh Maghool, Milad Shademan, Morteza Zahedi, Mehrdad Zeinalian, Foroogh Alborzi, Mohammad Reza Keramati, Ashkan Torshizian, Hassan Vosoghinia, Farnood Rajabzadeh, Alireza Bary, Massih Bahar, Ali Javadmanesh, Jamshid Sorouri-Khorashad, Mohammad Hassan Emami, Nasser Ebrahimi Daryani, Hans F.A. Vasen, Ladan Goshayeshi, Hesam Dehghani

“…Gene ontology and protein-protein interaction (PPI) analyses were conducted to elucidate gene roles on protein function. Results Pathogenic/likely pathogenic (P/LP) variants were identified in Lynch-related genes in 36.51% of patients. …”
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The efficacy, safety and clinical feasibility of a percutaneous atrial septal shunt device for pulmonary arterial hypertension: a single-center cohort study by Mingfei Li, Dandan Chen, Jianing Fan, Dan Tian, Lei Zhang, Xiaochun Zhang, Shasha Chen, Yuan Zhang, Wenzhi Pan, Lihua Guan, Daxin Zhou, Junbo Ge

“…Seven patients’ New York Heart Association (NYHA) functional classification improved from grade III to grade II. …”
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Measure selection for an electronic patient-reported outcome (ePRO) system for CAR T-cell therapy patients: a modified Delphi consensus studyResearch in context by Sarah E. Hughes, Foram Khatsuria, Christel McMullan, Karen L. Shaw, Anita Walker, Francesca Kinsella, David Burns, Olalekan L. Aiyegbusi, Elin Haf Davies, John Ansell, Evelyn Chakera, Charles Craddock, Alastair Denniston, Rebecca Lloyd, Paul Ferguson, Ronjon Chakraverty, Melanie Calvert

“…These measures, EQ5D-5L, measuring HRQoL, and Functional Assessment of Chronic Illness Therapy–Item GP5 (FACT-GP5), single-item global indicator of cancer treatment tolerability, will be included in the ePRO system. …”
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Regulatory roles of an sRNA derived from the 5´ UTR and sequence internal to lapA in Pseudomonas aeruginosa PAO1 by Xiaojuan Tan, Jingjing Xiao, Qianqian Liu, Ting Yang, Dandan Feng, Ruyi Zheng, Liping Luo, Xi Cheng, Dongsheng Du, Minghui Li, Jinwei Zhou, Guoping Zhu

“…However, some of these novel sRNAs have been functionally characterized. In this study, a previously uncharacterized sRNA, LapS, in P. aeruginosa PAO1 was identified as a novel sRNA. …”
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Novel trispecific killer engager targeting B7-H3 enhances natural killer cell antitumor activity against head and neck cancer by Jeffrey S Miller, Martin Felices, Peter Hinderlie, James Lim, Naomi Fujioka, Shee Kwan Phung, Yvette Soignier, Joshua T Walker, Melissa A Geller, Madison Shackelford, Carly Selleck, Philippa R Kennedy, Mihir Shetty, Melissa J Khaw, Paolo P Provenzano, Nicholas A Zorko, Laura E Bendzick, Riley C Lyons, Elise L Femino, Terran Stenger, Tumpa Dasgupta, Laura E Kotz, Eng Hock Lee, Quynhanh Lu, Zachary B Davis, John E Wagner, Margaret L MacMillan

“…This TriKE is comprised of a camelid nanobody that binds to CD16 on NK cells, an interleukin (IL)-15 moiety, and another novel camelid nanobody that binds to the B7-H3 antigen, which is highly and specifically expressed on the tumor cell surface.Methods The B7H3 TriKE was generated using a mammalian expression system. Its functionality was evaluated using flow cytometry-based NK cell degranulation, cytokine production, proliferation and live cell imaging cytotoxicity assays. …”
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Targeted hematopoietic stem cell depletion through SCF-blockade by Yan Yi Chan, Pui Yan Ho, Carla Dib, Leah Swartzrock, Maire Rayburn, Hana Willner, Ethan Ko, Katie Ho, Julian D. Down, Adam C. Wilkinson, Hiro Nakauchi, Morgane Denis, Taylor Cool, Agnieszka Czechowicz

“…Subsequent efforts have built upon this work to develop various combination approaches, though none are optimal and how any of these mAbs fully function is unknown. Methods To improve efficacy of mAb-based conditioning as a stand-alone conditioning approach for all HSCT settings, it is critical to understand the mechanistic action of αCD117 mAbs on HSCs. …”
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Geographic variation in secondary metabolites contents and their relationship with soil mineral elements in Pleuropterus multiflorum Thunb. from different regions by Yaling Yang, Siman Wang, Ruibin Bai, Feng Xiong, Yan Jin, Hanwei Liu, Ziyi Wang, Chengyuan Yang, Yi Yu, Apu Chowdhury, Chuanzhi Kang, Jian Yang, Lanping Guo

“…Among these, 2 important functional compounds including physcion and 2,3,5,4′-tetrahydroxystilbene-2-O-(6″-O-acetyl)-glucoside (THSG-5) are the most abundant in HSW samples from Deqing, a geographical indicative production region. …”
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A highly conserved sRNA downregulates multiple genes, including a σ54 transcriptional activator, in the virulence mode of Bordetella pertussis by Minji Sim, Jeffers Nguyen, Karolína Škopová, Kyungyoon Yoo, Chin-Hsien Tai, Leslie Knipling, Qing Chen, David Kim, Summer Nolan, Rawan Elaksher, Nadim Majdalani, Hernan Lorenzi, Scott Stibitz, Kyung Moon, Deborah M. Hinton

“…In the human pathogen Bordetella pertussis, the causative agent of whooping cough, hundreds of sRNA have been identified, but their functions remain largely unknown. We have characterized an sRNA that is abundant in the virulence mode of B. pertussis and serves to downregulate multiple genes, including transcriptional regulators and various transporters. …”
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Dynamic conditioning of porcine kidney grafts with extracellular vesicles derived from urine progenitor cells: A proof‐of‐concept study by Perrine Burdeyron, Sébastien Giraud, Maryne Lepoittevin, Nina Jordan, Sonia Brishoual, Maïté Jacquard, Virginie Ameteau, Nadège Boildieu, Estelle Lemarie, Jonathan Daniel, Frédéric Martins, Nicolas Mélis, Marine Coué, Raphaël Thuillier, Henri Leuvenink, Luc Pellerin, Thierry Hauet, Clara Steichen

“…However, cell damage markers (LDH, ASAT) were decreased in G3 compared with G1, and G3 kidneys displayed a preserved tissue integrity with reduced tubular dilatation and inflammation notably. However, renal function indicators such as creatinine clearance measured for 5 h of normothermic perfusion or NGAL perfusate's level were not modified by EVs injection. …”
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Low blood levels of selenium, selenoprotein P and GPx3 are associated with accelerated biological aging: results from the Berlin Aging Study II (BASE-II) by Valentin Max Vetter, Kamil Demircan, Jan Homann, Thilo Samson Chillon, Michael Mülleder, Orr Shomroni, Elisabeth Steinhagen-Thiessen, Markus Ralser, Christina M. Lill, Lars Bertram, Lutz Schomburg, Ilja Demuth

“…Abstract Background Biological age reflects inter-individual differences in biological function and capacity beyond chronological age. DNA methylation age (DNAmA) and its deviation from chronological age, DNAmA acceleration (DNAmAA), which was calculated as residuals of leukocyte cell count adjusted linear regression of DNAmA on chronological age, were used to estimate biological age in this study. …”
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To reveal biomarkers related to macrophage and lactic acid metabolism in renal fibrosis and explore their mechanisms by Chen Yong, Chen Yong, Yongfei Yu, Yongfei Yu, Yuan Wei, Yuan Wei, Guoshun Huang, Guoshun Huang, Lianghui Shu, Lianghui Shu, Kun Gao, Kun Gao, Kun Gao, Enchao Zhou, Enchao Zhou, Enchao Zhou, Enchao Zhou

“…IntroductionLactate can influence the fibrotic process by regulating cellular metabolism, inflammatory responses, and cell proliferation, which may be closely related to macrophage function in diseases. Therefore, this research sought to identify biomarkers linked to lactate metabolism and macrophages in renal fibrosis (RF).MethodsFirstly, key modular genes associated with macrophage score and lactate metabolism score were identified by combining single-sample gene set enrichment analysis (ssGSEA) and weighted gene co-expression network analysis. …”
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LKB1 dictates sensitivity to immunotherapy through Skp2-mediated ubiquitination of PD-L1 protein in non-small cell lung cancer by Wei Liu, Dong Wang, Qing Miao, Peilin Chen, Hongbing Liu, Jiawen Lv, Jie Yin, Jian Feng, Yong Song, Tangfeng Lv, Mingxiang Ye, Wenjie Yan, Liting Lv, Sutong Zhan

“…Background Loss-of-function mutations of liver kinase B (LKB1, also termed as STK11 (serine/threonine kinase 11)) are frequently detected in patients with non-small cell lung cancer (NSCLC). …”
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Repertoire-based mapping and time-tracking of T helper cell subsets in scRNA-Seq by Daniil K. Lukyanov, Daniil K. Lukyanov, Valeriia V. Kriukova, Kristin Ladell, Irina A. Shagina, Irina A. Shagina, Dmitry B. Staroverov, Dmitry B. Staroverov, Bella E. Minasian, Anna S. Fedosova, Pavel Shelyakin, Oleg N. Suchalko, Alexander Y. Komkov, Konstantin A. Blagodatskikh, Kelly L. Miners, Olga V. Britanova, Olga V. Britanova, Olga V. Britanova, Andre Franke, David A. Price, David A. Price, Dmitry M. Chudakov, Dmitry M. Chudakov, Dmitry M. Chudakov, Dmitry M. Chudakov, Dmitry M. Chudakov

“…IntroductionThe functional programs of CD4+ T helper (Th) cell clones play a central role in shaping immune responses to different challenges. …”
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Dynamic regulation of Rgs16 and its correlation with Neuregulin1 expression in acute and chronic nerve injury by Marina García-Bejarano, Marina García-Bejarano, Marina García-Bejarano, Riccardo Aucello, Riccardo Aucello, Federica Zen, Federica Zen, Marwa El Soury, Marwa El Soury, Francesca Cordero, Jesús M. de la Fuente, Isabelle Perroteau, Giulia Ronchi, Giulia Ronchi, Giovanna Gambarotta, Giovanna Gambarotta

“…Further investigations are needed to clarify RGS16 function, which could pave the way for novel therapeutic strategies to enhance nerve regeneration and alleviate neuropathic pain.…”
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Catheter ablation of atrial fibrillation in women with heart failure with preserved ejection fraction by Maura M. Zylla, Maura M. Zylla, Maura M. Zylla, Johannes Leiner, Johannes Leiner, Johannes Leiner, Ann-Kathrin Rahm, Ann-Kathrin Rahm, Ann-Kathrin Rahm, Tobias Hoffmann, Tobias Hoffmann, Tobias Hoffmann, Patrick Lugenbiel, Patrick Lugenbiel, Patrick Lugenbiel, Patrick Schweizer, Patrick Schweizer, Patrick Schweizer, Christine Mages, Christine Mages, Christine Mages, Derliz Mereles, Derliz Mereles, Derliz Mereles, Meinhard Kieser, Eberhard Scholz, Eberhard Scholz, Eberhard Scholz, Hugo A. Katus, Hugo A. Katus, Hugo A. Katus, Norbert Frey, Norbert Frey, Norbert Frey, Dierk Thomas, Dierk Thomas, Dierk Thomas

“…Women with HFpEF still exhibited a lower SF-36 Physical Component Summary Score vs. women without HFpEF (median: 41.2 vs. 52.1, P < 0.001).ConclusionWomen with HFpEF constitute a distinct subgroup with high rates of AF-related events after AF-ablation, and persistence of both symptoms and functional hallmarks of HFpEF. Consideration of sex-specific cardiac co-morbidities is crucial for personalization and optimization of AF-therapy.Clinical Trial RegistrationClinicalTrials.gov Identifier NCT05603611.…”
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Molecular determinants of skeletal muscle force loss in response to 5 days of dry immersion in human by Mathias Velarde, Michel‐Yves Sempore, Valentine Allibert, Valérie Montel, Josiane Castells, Loïc Treffel, Angèle Chopard, Thomas Brioche, Laetitia Cochon, Jérome Morel, Bruno Bastide, Anne‐Cécile Durieux, Laurence Stevens, Damien Freyssenet

“…Abstract Background Astronauts in Earth's orbit experience microgravity, resulting in a decline of skeletal muscle mass and function. On Earth, models simulating microgravity have shown that the extent of the loss in muscle force is greater than the loss in muscle mass. …”
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Impaired response inhibition to negative emotional stimuli in depressed adolescents with non-suicidal self-injury: a neurophysiological perspective by Lin Zhao, Su Hong, Xinyu Peng, Xiaoqing He, Jinhui Hu, Lingli Ma, Xinyi Liu, Wanqing Tao, Ran Chen, Zhenghao Jiang, Chenyu Zhang, Jing Liao, Jiaojiao Xiang, Qi Zeng, Linqi Dai, Qi Zhang, Wo Wang, Li Kuang

“…Moreover, depressed adolescents with NSSI showed significant event-related synchronization (ERS) in the Delta and Theta bands of FCz electrode from 0 to 0.6 seconds, and event-related desynchronization (ERD) in the Theta and Alpha bands of Pz electrode from 0.2 to 1.2 seconds, collectively reflecting functional processes associated with response inhibition.ConclusionsDepressed adolescents with NSSI showed increased P3d amplitudes, enhanced Delta/Theta ERS, and heightened Theta/Alpha ERD when receiving negative emotional stimuli, suggesting that depressed adolescents with NSSI have impaired response inhibition, which may contribute to the development of NSSI.…”
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Metabolome and Transcriptome Analysis Reveals the Regulatory Effect of Magnesium Treatment on EGCG Biosynthesis in Tea Shoots (<i>Camellia sinensis</i>) by Zixuan Feng, Zhuan Li, Rui Yan, Nan Yang, Meichen Liu, Yueting Bai, Yuyuan Mao, Chengzhe Zhou, Yuqiong Guo, Yulin Zeng, Yuhang Ji, Yangshun Lin, Jiayong Chen, Shuilian Gao

“…Epigallocatechin-3-O-gallate (EGCG) is an important ingredient that indicates tea quality and has healthcare functions. Magnesium nutrition can improve the quality and yield of tea plants, but its regulatory role in the biosynthesis of EGCG in tea plants has not been clarified. …”
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In PD-1+ human colon cancer cells NIVOLUMAB promotes survival and could protect tumor cells from conventional therapies by Nicola Normanno, Fabiana Tatangelo, Piera Maiolino, Stefania Scala, Caterina Ieranò, Dario Righelli, Crescenzo D'Alterio, Maria Napolitano, Luigi Portella, Giuseppina Rea, Federica Auletta, Sara Santagata, Anna Maria Trotta, Giuseppe Guardascione, Federica Liotti, Nella Prevete, Antonio Luciano, Antonio Barbieri, Annabella Di Mauro, Cristin Roma, Riziero Esposito Abate, Roberto Pacelli, Rosa Marina Melillo

“…In melanoma, liver, and thyroid cancer cells, intrinsic PD-1 signaling activates oncogenic functions, while in lung cancer cells, it has a tumor suppressor effect. …”
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Minimally Invasive Transthoracic Intramyocardial Cellular Transplantation Under Echocardiographic Guidance for Myocardial Impairment by Sulei Li, Bo Tao, Junsong Liu, Dongyue Liu, Yujia Wang, Jibin Zhang, Lei Tian, Baixuan Xu, Yan Fang, Yabin Wang, Xin Lou, Jinming Zhang, Feng Lan, Feng Cao, Hanjia Gao, Xiaoxia Fu

“…The operation time, postoperative recovery time of animals and volume of blood loss were collected for comparison between MI-SIM group and MI-TTEPIM group. 9-(4-[18F] fluoro-3-(hydroxymethyl) butyl) guanine positron emission tomography/computed tomography imaging was performed to track the hiPS-CMTK+in vivo. Cardiac function and morphology were evaluated by echocardiography. …”
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