Systematic approach for dissecting promoters and designing transform systems in microalgae by Qinhua Gan, Xinjun Yu, Xiang Jiang, Xingwei Huang, Yi Xin, Yandu Lu

“…Assessment of the in vivo activity revealed the maximum transformation efficiency (414 ± 102 transformants/µg DNA) in the plasmid pHN5-2 containing lhp promoter and α-tub terminator. …”
Get full text

A YOLO11-Based Method for Segmenting Secondary Phases in Cu-Fe Alloy Microstructures by Qingxiu Jing, Ruiyang Wu, Zhicong Zhang, Yong Li, Qiqi Chang, Weihui Liu, Xiaodong Huang

“…To address the issues of subjectivity, low efficiency, and limited quantitative capability in traditional metallographic analysis methods, this paper proposes a deep learning-based approach for segmenting the second phase in Cu-Fe alloys. …”
Get full text

Hyperspectral Image Mixed Denoising via Robust Representation Coefficient Image Guidance and Nonlocal Low-Rank Approximation by Jiawei Song, Baolong Guo, Zhe Yuan, Chao Wang, Fangliang He, Cheng Li

“…Extensive experiments conducted on both simulated and real HSI datasets demonstrate that the proposed robust RCIs guidance and low-rank approximation method, denoted as RRGNLA, exhibits competitive performance in terms of mixed denoising accuracy and computational efficiency. For instance, on the Washington DC Mall (WDC) dataset in Case 3, the denoising quantitative metrics of the mean peak signal-to-noise ratio (MPSNR), mean structural similarity index (MSSIM), and spectral angle mean (SAM) are 36.06 dB, 0.963, and 3.449, respectively, with a running time of 35.24 s. …”
Get full text

A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection. by Martin A M Reijns, Louise Thompson, Juan Carlos Acosta, Holly A Black, Francisco J Sanchez-Luque, Austin Diamond, David A Parry, Alison Daniels, Marie O'Shea, Carolina Uggenti, Maria C Sanchez, Alan O'Callaghan, Michelle L L McNab, Martyna Adamowicz, Elias T Friman, Toby Hurd, Edward J Jarman, Frederic Li Mow Chee, Jacqueline K Rainger, Marion Walker, Camilla Drake, Dasa Longman, Christine Mordstein, Sophie J Warlow, Stewart McKay, Louise Slater, Morad Ansari, Ian P M Tomlinson, David Moore, Nadine Wilkinson, Jill Shepherd, Kate Templeton, Ingolfur Johannessen, Christine Tait-Burkard, Jürgen G Haas, Nick Gilbert, Ian R Adams, Andrew P Jackson

“…The most sensitive test involves the detection of viral RNA using RT-qPCR (quantitative reverse transcription PCR), with many commercial kits now available for this purpose. …”
Get full text

Enhancing Cloud Detection in Polar Regions Using Combined Spectral and Textural Features for Landsat 8/9 OLI Imagery by Xinran Shen, Teng Li, Chong Liu, Shaoyin Wang, Lei Zheng, Qi Liang, Xiao Cheng, Jiaqi Yao

“…Remote sensing is a cost-effective and efficient method for studying polar regions. However, cloud cover in optical remote sensing images can diminish the data integrity for certain snow/ice applications. …”
Get full text

Design and psychometric evaluation of the Emergency Medical Services Safe Care Scale (EMSSCS): a mixed-method study by Fateme Mohammadi, Rasoul Salimi, Mohammadreza Shokouhi, Salman Khazaei, Mostafa Bijani

“…Results Safe care provided by EMS personnel was defined as the management of the incident scene, efficient clinical skills, and effective interaction aimed at delivering principled and safe patient care. …”
Get full text

Influence of diffusion siliconizing and boron–siliconizing in chlorine-containing environments on the structure, composition, and protective properties of TZM molybdenum alloy by Tetiana Loskutova, Michael Scheffler, Vitalii Ivanov, Manja Krueger, Nadiia Kharchenko, Volodymyr Taran, Michal Hatala, Myroslav Karpets, Yaroslav Stelmakh, Georg Hasemann

Get full text

Effectiveness of Irrigation Protocols in Endodontic Therapy: An Umbrella Review by Manuel J. Orozco-Gallego, Eliana L. Pineda-Vélez, Wilder J. Rojas-Gutiérrez, Martha L. Rincón-Rodríguez, Andrés A. Agudelo-Suárez

“…Enhanced activation methods, such as PUI and lasers, improve irrigant efficiency, while alternatives like chlorhexidine (CHX) offer better biocompatibility. …”
Get full text

High performance thin layer chromatography with eluents containing SDS aided by UV- and Raman spectroscopy by Beata Polak, Adrianna Kryska, Kamila Jaglińska, Adam Traczuk, Barbara Barwinek

“…For four of the six investigated compounds, these factors have been close to 1.0. The separation efficiency measured by the height of the theoretical plate varies from 39 to 73 μm depending on the solute investigated. …”
Get full text

Evaluation methods of artificial demineralization protocols for coronal dentin: a systematic review of laboratory studies by Alaa Esmat Abdel Salam, Hoda Saleh Ismail, Hamdi Hamama

“…Furthermore, this evidence-based study was conducted to identify the most reliable evaluation methods in assessing the efficiency of the reviewed protocols. Materials and methods An electronic search was conducted on three databases: MEDLINE/PubMed, Scopus, and ScienceDirect, following PRISMA guidelines. …”
Get full text

Productive and reproductive losses associated with abortion in specialized dairy cattle from Costa Rica by Emilia Vindas-van der Wielen, Juan José Romero-Zúñiga, Gustavo Monti

Get full text

A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy by Andreas Schulte, Janik Brockmann, Nina Müller, Tibor Anderlei, Jochen Büchs

“…Abstract Background Shake flasks are essential tools in biotechnological development due to their cost efficiency and ease of use. However, a significant challenge is the miniaturization of process analytical tools to maximize information output from each cultivation. …”
Get full text

A method for determining fracturing radius of blasting holes based on SF6 gas tracing by Dongxu JIA

“…The fracturing radius, as a key indicator characterizing the energy transfer of mine blasting and the fragmentation effect of rock mass, its precise determination is directly related to the safety and energy efficiency of blasting engineering. However, due to the heterogeneity of complex geological structures, the irreversibility of the dynamic crack evolution mechanism and the limitations of in-situ monitoring technology, there are still significant technical bottlenecks in the in-situ detection of the fracturing radius at present. …”
Get full text

Modern Technologies to Detect Fungi That Cause Diseases to Agricultural Crops: A review by Teeba H. Mohammad, Zahraa A. Abed, Zahraa R. Taha

“…The traditional detection of pathological fungi yields dubious and inaccurate results, which do not facilitate effective treatment against this pathogenic fungus. It has proven its efficiency in accurately and rapidly diagnosing fungal species and diseases that do not exhibit symptoms. …”
Get full text

BEHAVIOUR OF INDIVIDUAL CONSUMERS AS AN OBJECT MANAGEMENT INTERESTS by Yurii Y. Petrunia, Vira Y. Petrunia, Olexander I. Guba

“…By contrast, the health condition affects the level of “social happiness” (social welfare), efficiency and productivity of social work (ability to perform a certain amount of work and provide a certain level of intensity), the corresponding costs of the state and local budgets connected with the prevention and treatment of diseases, the use of various compensation payments related to the health problems. …”
Get full text

A critical analysis of public private partnership model in energy from waste projects by Junaid Tahir, Mark Atkinson, Zhigang Tian, Mohamad Kassem, Rafiq Ahmad, Pablo Martinez

“…Governments and investors are increasingly questioning their fiscal efficiency and overall benefit to taxpayers. Despite these concerns, studies investigating whether PFI provides value of money of PFI in EfW are lacking. …”
Get full text

Digital Transformation for Sustainability in Industry 4.0: Alleviating the Corporate Digital Divide and Enhancing Supply Chain Collaboration by Qi Li, Weijian Tian, Hua Zhang

“…These advancements hold the potential to improve efficiency, reduce waste, and foster sustainable development; however, they also create challenges due to the uneven adoption of digital technologies across enterprises. (1) Background: The adoption of digital technologies across supply chains is uneven, resulting in a digital divide between enterprises. …”
Get full text

A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products by Wen Cui, Liang Zhao, Yuwen Shi, Wei Feng, Xiao Chen, Hui Liu

“…In conclusion, we have developed a novel and effective disposable product for estimating efficiency of the culture medium.…”
Get full text

A New Parameter-Free Slope Unit Division Method That Integrates Terrain Factors by Ping Li, Junfu Fan, Yujie Du, Kuan Li, Yuke Zhou

“…Despite the significant progress in slope unit division techniques, most existing methods still have certain limitations, such as a strong dependence on manually set thresholds during the division process, resulting in low levels of automation and efficiency. To address this issue, a new parameter-free slope unit extraction algorithm that integrates terrain factors, called Terrain Factor Parameter-Free Slope Unit Division (TFPF-SU), is introduced. …”
Get full text

Resilience Assessment in a Seawater Pumping Station Using Ordinal Patterns and Permutation Entropy Approach by Orlando Durán, Christian Salas, Alejandro Peña

“…Resilience is framed as the system’s capacity to recover from disruptions and maintain operational efficiency under varying conditions. Quantitative assessment of resilience is essential to facilitate the development and implementation of optimal strategies that ensure operational continuity. …”
Get full text